| Project/Area Number |
17592088
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokushima |
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Principal Investigator |
BANDO Takashi The University of Tokushima, Institute of Health Biosciences, Assistant Professor, 大学院ヘルスバイオサイエンス研究部, 助手 (80208727)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | maspin / salivary gland / myoepithelial cell |
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| Research Abstract |
1) Human salivary myoepithelial cells (NS-SV-MC). immortalized by the transfection of mutant SV-40 DNA, with high or low expression of maspin and were grown with human salivary gland cancer cells (HSG) in vitro or in vivo (nude mice). As a result maspin produced by NS-SV-MC inhibited the growth, invasion, and metastasis to cervical lymph nodes of HSG cells while it did not affect the differentiation of them.
2) It is reported that maspin binds to interferon regulatory factor-6 (IFR-6), one of the transcriptional factors (J. Biol. Chem.. 280. 34210. 2005). Then the expression and the localization of maspin and IRF-6 in NS-SV-MC and human salivary duct cells (NS-SV-DC). immortalized by the transfection of mutant SV-40 DNA. was examined. The results indicated that IFR-6 was expressed in the nuclei of NS-SV-MC with low expression of maspin while IFR-6 and maspin were co-localized in the cytoplasm of NS-SV-DC. Therefore. it was suggested that endogenous maspin was associated with the differentiation to myoepithelium via binding to IRF-6.
3) It was previously suggested that human malignant salivary gland tumors (adenoid cystic carcinoma) express less maspin while benign ones express maspin. Here the sequence of tumor suppressor gene p53, which regulates the transcription of maspin gene. and cytosine-methylation in the maspin promoter region were investigated in adenoid cystic carcinoma. As a result 9 methylated cytosines in 13 CpG islands of maspin promoter (-296〜 + 184) were detected and the mutation in exon 5〜8 of p53 were not. Theses results suggested that methylation in the maspin promoter region might regulate the gene expression of maspin in adenoid cystic carcinoma.
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