| Project/Area Number |
17592094
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Surgical dentistry
|
|---|
| Research Institution | Nara Medical University |
|---|
Principal Investigator |
FUJIMOTO Masaki Nara Medical University, Department of medicine, RESEARCH ASSOCIATE, 医学部, 助手 (10316078)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMAMOTO Kazuhiko Nara Medical University, Department of medicine, ASSISTANT PROFESSOR, 医学部, 助教授 (20243842)
KIRITA Tadaaki Nara Medical University, Department of medicine, PROFESSOR, 医学部, 教授 (70201465)
|
|---|
| Project Period (FY) |
2005 – 2006
|
| Project Status |
Completed (Fiscal Year 2006)
|
| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,200,000 (Direct Cost: ¥2,200,000)
|
|---|
| Keywords | regenerative medicine / bone regeneration / basic fibroblast growth factor / bone morphogenetic protein / β-tricalcium phosphate |
|---|
| Research Abstract |
In late years dental implantation becomes apply in recovery of an occlusion function, and. reconstruction of alveolar bone of that purpose holds the important role.
Purpose:
In this study, we examined the efficacy of bone morphogenetic protein ( rh-BMP ) and basic fibroblast growth factor ( bFGF ) incorporated β-tricalcium phosphate ( β-TCP ) 'which was an absorbable bone substitute material in vivo on ontogenesis in a diffusion chamber ( DC ) to develop a bone substitute having consistent osteogenic capability for clinical applications. Materials and methods:
We prepared six groups of DCs: a;β-TcP + isotonic sodium chloride solution, b;13-TCP +bFGF 1 μg, c;β-TCP +bFGF 10μg, d-;β-TCP +BMP 10μg, e;β-TCP +bFGF.1 pg +BMP 1Oμg, f;β-TCP +bFGF 10μg +BM.P 10μg. The prepared solutions were injected into DCs, which were subcutaneously implanted into the backs of F344 rats. DCs were harvested after 2,3,4,8,16 or 24 weeks and analyzed for bone forming capability by determining histological and biochemical markers.
Result:
We observed the formation of de novo bone in their groups to contain rh-BMP histologically since the second week, and the formation of active new bone was noted since three weeks. In the particularly bone neogenesis in the part which contacted with direct organism on the membrane outside of DCs was remarkable in the group "d". The alkaline phosphatase activity and osteocalcine content in the group of "d" and "e" were significantly higher than other groups at any time.
Conclusion:
It is suitable for an optimum condition in bone neogenesis in this study model, which added rh-BMP (0.5 μg/μ1) incorporated β-TCP and then bFGF of low concentration (0.05 μg/μ1).
|
