Development of novel therapy for TMJ arthritis by Gene transfer using with sonoporation combined with microbubble-based ultrasound contrast agent

• Project/Area Number: 17592097
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Kyushu Dental College
• Principal Investigator: TOMINAGA Kazuhiro Kyushu Dental College, Dept. of Oral and Maxillofacial Surgery, Associate Professor (40188793)
• Co-Investigator(Kenkyū-buntansha): NISHIBARA Tatsuji Kyushu Dental College, Dept of Health Promotion, Professor (80192251)
• Project Period (FY): 2005 – 2006
• Project Status: Completed (Fiscal Year 2006)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,500,000); Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000); Fiscal Year 2005: ¥2,600,000 (Direct Cost: ¥2,600,000)
• Keywords: temporomandibular disorder / ultrasonographic gene induction / interleukin-1 / IL-1 receptor antagonist / svnovial cells / microbubble / PGE2 / anti-inflammatory effect / アジュバント関節炎 / IL1-ra

Research Abstract

Administration of exogenous IL-1 receptor antagonist (IL-1ra) is effective in reducing the inflammatory reactions mediated by IL-1. In the present study, we transfected with a plasmid containing IL-1ra gene into synovial cells by the sonoporation method, and investigated the effect of IL-1ra gene transfection on inflammatory reactions induced by lipopolysaccharide (LPS) in vitro. IL-1ra- transfected synovial cells produced the extracellular IL-lra, and its level increased remarkably when the cells were cultured with LPS for 48 h. Interestingly, treatment of LPS enhanced the release of IL-1β in the culture supernatant of IL-1ra-transfected cells, however, a significant decrease of PGE2 release was observed when the cells were cultured with LPS for 60 h. These results indicate that IL-1ra could play a valuable regulatory role in inflammatory reactions of synovial cells, suggesting that the administration of IL-1ra gene with sonoporation is a useful method for the control of inflammation in arthritis.

Research Products

• [Journal Article] Relationship between cortical bone formation on mandibular condyles and alternation of the magnetic resonance signals characteristic during growth (2007)
  • Author(s): Morimoto, Y.
  • Journal Title: Am J Orthod Dentofacial Orthop 131 Pages: 473-480
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Local delivery system of cytotoxic agents to tumors by focued sonoporation (2007)
  • Author(s): Iwanaga, K.
  • Journal Title: Cancer Gene Therapy 14 Pages: 354-363
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Intraoperative surgical clearance confirmation of tongue carcinomas using ultrasonography (2007)
  • Author(s): Tominaga, K.
  • Journal Title: Dentomaxillofac Radiol 36 Pages: 409-411
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Changes of Temporomandibular Disk Position during growth in young Japanese (2007)
  • Author(s): Tominaga, K.
  • Journal Title: Dentomaxillofac Radiol 36 Pages: 397-401
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Fukuda, j.and Ohba, T. : Relationship between cortical bone formation on mandibular condyles and alternation of the magnetic resonance signals characteristic of growth. (2007)
  • Author(s): Morimoto, Y., Konoo, T., Tominaga, K., Tanaka, T., Yamaguchi, K., Fukuda, j. Ohba, T.
  • Journal Title: Am J Orthod Dentofacial Orthop 131(4) Pages: 473-480
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Local delivery system of cytotoxic agents by focued sonoporation. (2007)
  • Author(s): Iwanaga, K., Tominaga, K., Yamamoto, K., Habu, M., Maeda, H., Akifusa, S., Tsujusawa, T., Okinaga, T., Fukuda, J., Nishihara, ' T.
  • Journal Title: Cancer Gene Therapy 14 Pages: 351-363
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Intraoperative surgical clearance confirmation of tongue carcinomas using ultrasonography. (2007)
  • Author(s): Tominaga, K., Yamamoto, K., Khanal, A., Morimoto, Y., Tanaka, T., Kodama, M., Fukuda, J
  • Journal Title: Dentomaxillofac Radiol 36 Pages: 409-411
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Changes in temporomandibular disc position during growth in young Japanese. (2007)
  • Author(s): Tominaga, K., Konoo, T., Morimoto, Y., Tanaka, T., Habu, M. Fukuda, J.
  • Journal Title: Dentomaxillofac Radiol 36 Pages: 397-401
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A simple method for bone positioning of mandibular segments (2006)
  • Author(s): Tominaga, K.
  • Journal Title: Int J Oral Maxillofac Surg 35 Pages: 856-860
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] Biomechanical evaluation of different types of rigid internal fixation techniques for subcondylar fractures (2006)
  • Author(s): Tominaga, K.
  • Journal Title: J Oral Maxillofac Surg 41 Pages: 1510-1516
  • Description: 「研究成果報告書概要(和文)」より
• [Journal Article] A simple method for bone positioning of mandibular segments. (2006)
  • Author(s): Tominaga, K., Yoshioka, I., Khanal, A., Furuta, N., Habu, M. Fukuda, J
  • Journal Title: mt J Oral Maxillofac Surg 35 Pages: 856-860
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Biomechanical evaluation of different types of rigid internal fixation techniques for subcondylar fractures. (2006)
  • Author(s): Tominaga, K., Habu, M., Khanal, A., Mimori, Y., Yoshioka, I. Fukuda, J.
  • Journal Title: J Oral Maxillofac Surg 41 Pages: 1510-1516
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A simple method for bone positioning of mandibular segments. (2006)
  • Author(s): Tominaga, K.
  • Journal Title: Int J Oral Maxillofac Surg 36 Pages: 856-860
• [Journal Article] Biomechanical evaluation of different types of rigid internal fixation techniques for subcondylar fractures. (2006)
  • Author(s): Tominaga, K.
  • Journal Title: J Oral Maxillofac Surg 41 Pages: 1510-1516
• [Journal Article] Relationship between cortical bone formation on mandibular condyles and alternation of the magnetic resonance signals characteristic during growth. (2006)
  • Author(s): Morimoto, Y.
  • Journal Title: Am J Orthod Dentofacial Orthop (in press)
• [Journal Article] Local delivery system of cytotoxic agent by sonoporation. (2006)
  • Author(s): Iwanaga, K.
  • Journal Title: Cancer Gene Therapy (in press)
• [Journal Article] Intraoperative surgical clearance confirmation of tongue carcinomas using ultrasonography (2006)
  • Author(s): Tominaga, K.
  • Journal Title: Dentomaxillofac Radiol (in press)
• [Journal Article] Changes of Temporomandibular Disk Position during growth in young Japanese. (2006)
  • Author(s): Tominaga, K.
  • Journal Title: Dentomaxillofax Radiol (in press)
• [Journal Article] Utility of three dimension fast asymmetric spin-echo (3D-FASE) sequences in MR sialographic sequences : model and volunteer studies. (2005)
  • Author(s): Morimoto, Y.
  • Journal Title: Oral Disease 11(1) Pages: 35-43
• [Journal Article] The morphological features of the cartilage during distraction osteogenesis in rabbit mandible. (2005)
  • Author(s): Furuta, N.
  • Journal Title: Dent Jpn 41 Pages: 70-74
• [Journal Article] Three-dimensional identification of hemangiomas and feeding arteries in the head and neck region using combined phase-contrast MR angiography and fast asymmetric spin-echo sequences. (2005)
  • Author(s): Tanaka, T.
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod 100 Pages: 609-613
• [Journal Article] Relationship between TNF- α andTUNEL-positive chondrocytes in antigen-induced arthritis of the rabbit temporomandibular joint. (2005)
  • Author(s): Hirota, Y.
  • Journal Title: J Oral Pathol Med 35 Pages: 1-8