Radical Production and Induction of Apoptosis for Phenolic Antioxidants
| Project/Area Number |
17592104
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Meikai University |
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Principal Investigator |
OKADA Norihisa Meikai University, School of Dentistry, Diagnostic & Therapeutic Sciences, Assistant Professor, 歯学部, 講師 (40146220)
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| Co-Investigator(Kenkyū-buntansha) |
FUJISAWA Seiichiro Meikai University, School of Dentistry, Professor Emeritus, 歯学部, 名誉教授 (40014162)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Phenol / Antioxidant / Radical / Apoptosis / ユージノール / イソユージノール |
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| Research Abstract |
Eugenol (EUG) is used as ZOE cements in dentistry. EUG and its isomer (IsoEUG) generate reactive oxygen species (ROS) by photooxidation. We investigated cytotoxic effects of visible light (VL) irradiated EUG and IsoEUG on oral mucous membranes of mice. Tissue damage in IsoEUG samples was markedly greater than in EUG samples even without VL-irradiation and the potency of toxicity for both compounds was further enhanced by VL irradiation. Histopathological examination using H＆E stain and TUNEL method suggested that the biological event characterized was predominantly a necrosis, not an apoptosis.
Next, we investigated cytotoxic effects photoinitiators, camphorquinone (CQ) and 9 fluorenone (9F) on oral mucosa of mice. The buccal epithelium treated with CQ showed marked thickening of pricle cell layer due to cellular edema, indicating acute inflammation. Similar observations were found in 9F. VL-irradiated CQ and 9F showed much more severe inflammation than non-irradiated ones. The induction of apoptosis was not found in CQ and 9F with and without VL irradiation. To evaluate the protective effects of phenolic compounds such as vitamin E the radical-scavenging activity of alpha-tocopherol (TOC), beta-TOC, gamma-TOC and delta-TOC was investigated using the induction period method. Alkyl and peroxy radicals were scavenged by these tocopherols. Oxidative stress by photoinitiators and eugenol-related compounds with VL irradiation may be suppressed by co-antioxidants tocopherols, glutathione, ascorbates in vivo.
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Report
(3 results)
Research Products
(23 results)
