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Radical Production and Induction of Apoptosis for Phenolic Antioxidants

Research Project

Project/Area Number 17592104
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionMeikai University

Principal Investigator

OKADA Norihisa  Meikai University, School of Dentistry, Diagnostic & Therapeutic Sciences, Assistant Professor, 歯学部, 講師 (40146220)

Co-Investigator(Kenkyū-buntansha) FUJISAWA Seiichiro  Meikai University, School of Dentistry, Professor Emeritus, 歯学部, 名誉教授 (40014162)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥2,500,000 (Direct Cost: ¥2,500,000)
Fiscal Year 2006: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
KeywordsPhenol / Antioxidant / Radical / Apoptosis / ユージノール / イソユージノール
Research Abstract

Eugenol (EUG) is used as ZOE cements in dentistry. EUG and its isomer (IsoEUG) generate reactive oxygen species (ROS) by photooxidation. We investigated cytotoxic effects of visible light (VL) irradiated EUG and IsoEUG on oral mucous membranes of mice. Tissue damage in IsoEUG samples was markedly greater than in EUG samples even without VL-irradiation and the potency of toxicity for both compounds was further enhanced by VL irradiation. Histopathological examination using H&E stain and TUNEL method suggested that the biological event characterized was predominantly a necrosis, not an apoptosis.
Next, we investigated cytotoxic effects photoinitiators, camphorquinone (CQ) and 9 fluorenone (9F) on oral mucosa of mice. The buccal epithelium treated with CQ showed marked thickening of pricle cell layer due to cellular edema, indicating acute inflammation. Similar observations were found in 9F. VL-irradiated CQ and 9F showed much more severe inflammation than non-irradiated ones. The induction of apoptosis was not found in CQ and 9F with and without VL irradiation. To evaluate the protective effects of phenolic compounds such as vitamin E the radical-scavenging activity of alpha-tocopherol (TOC), beta-TOC, gamma-TOC and delta-TOC was investigated using the induction period method. Alkyl and peroxy radicals were scavenged by these tocopherols. Oxidative stress by photoinitiators and eugenol-related compounds with VL irradiation may be suppressed by co-antioxidants tocopherols, glutathione, ascorbates in vivo.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (23 results)

All 2007 2006 2005

All Journal Article (23 results)

  • [Journal Article] Radical-scavenging activity of natural methoxyphenols vs. synthetic ones using the induction period method.2007

    • Author(s)
      Y.KADOMA, N.OKADA
    • Journal Title

      Molecules 12

      Pages: 130-138

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Radical-scavenging activity of natural methoxyphenols vs. synthetic ones using the induction period method.2007

    • Author(s)
      Y.KADOMA, N.OKADA et al.
    • Journal Title

      Molecules 12

      Pages: 130-138

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Free radical interaction between vitamin E (alpha-, beta-, gamma-and delta-tocopherol), ascorbate and flavonoids.2006

    • Author(s)
      Y.KADOMA, N.OKADA
    • Journal Title

      In Vivo 20・6B

      Pages: 823-827

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparative study of the alkyl and peroxy radical scavenging activities of polyphenols.2006

    • Author(s)
      S.FUJISAWA
    • Journal Title

      Chemosphere 62・1

      Pages: 71-79

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Free radical interaction between vitamin E (alpha-, beta-, gamma-and delta-tocopherol), ascorbate and flavonoids.2006

    • Author(s)
      Y.KADOMA, N.OKADA
    • Journal Title

      In Vivo 20-6B

      Pages: 823-827

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Comparative study of the alkyl and peroxy radical scavenging activities of polyphenols.2006

    • Author(s)
      S.FUJISAWA
    • Journal Title

      Chemosphere 62-1

      Pages: 71-79

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Free radical interaction between vitamin E (alpha-, beta-, gamma- and delta-tocopherol), ascorbate and flavonoids.2006

    • Author(s)
      Y.KADOMA, N.OKADA et al.
    • Journal Title

      In vivo 20・5

      Pages: 823-827

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Kinetic radical-scavenging activity of platonin, a cyanine photosensitizing dye.2006

    • Author(s)
      M.ISHIHARA, S.FUJISAWA et al.
    • Journal Title

      In vivo 20・6B

      Pages: 845-848

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A Quantitative approach to the free radical interaction between alpha-tocopherol or ascorbate and flavonoids.2006

    • Author(s)
      S.FUJISAWA et al.
    • Journal Title

      Invivo 20・4

      Pages: 445-452

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Induction of early apoptosis and ROS-generation activity in human gingival fibroblasts (HGF) and human submandibular gland carcinoma (HSG) cells treated with curcumin.2006

    • Author(s)
      T.ATSUMI, S.FUJISAWA et al.
    • Journal Title

      Arch Oral Biol 51・10

      Pages: 913-921

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Kinetic radical-scavenging activity of melatonin.2006

    • Author(s)
      S.FUJISAWA et al.
    • Journal Title

      Invivo 20・2

      Pages: 215-220

    • NAID

      120005520716

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comparative study of the alkyl and peroxy radical scavenging activities of polyphenols2006

    • Author(s)
      Seiichiro FUJISAWA
    • Journal Title

      Chemosphere 62・1

      Pages: 71-79

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Induction of cytotoxicity and apoptosis and inhibition of cyclo-oxygenase-2 gene expression by eugenol-related compounds.2005

    • Author(s)
      N.OKADA, S.FUJISAWA
    • Journal Title

      Anticancer Research 25・5

      Pages: 3263-3269

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of visible light irradiation on eugenol-treated oral mucosa.2005

    • Author(s)
      S.FUJISAWA, N.OKADA
    • Journal Title

      Dental Materials Journal 24・2

      Pages: 202-206

    • NAID

      110002983757

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A comparative study of the antioxidant/prooxidant activities of eugenol and isoeugenol with various concentrations and oxidation conditions.2005

    • Author(s)
      T.ATSUMI, S.FUJISAWA
    • Journal Title

      Toxicology In Vitro 19・8

      Pages: 1025-1033

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Induction of cytotoxicity and apoptosis and inhibition of cyclooxygenase-2 gene expression by eugenol-related compounds.2005

    • Author(s)
      N.OKADA, S.FUJISAWA
    • Journal Title

      Anticancer Research 25-5

      Pages: 3263-3269

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Effects of visible light irradiation on eugenol-treated oral mucosa.2005

    • Author(s)
      S.FUJISAWA, N.OKADA
    • Journal Title

      Dental Materials Journal 24-2

      Pages: 202-206

    • NAID

      110002983757

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A comparative study of the antioxidant/prooxidant activities of eugenol and isoeugenol with various concentrations and oxidation conditions.2005

    • Author(s)
      T.ATSUMI, S.FUJISAWA
    • Journal Title

      Toxicology In Vitro 19-8

      Pages: 1025-1033

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Induction of cytotoxicity and apoptosis and inhibition of cyclooxygenase-2 gene expression by eugenol-related compounds2005

    • Author(s)
      Norihisa OKADA
    • Journal Title

      Anticancer Research 25・5

      Pages: 3263-3269

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Effects of visible light irradiation on eugenol-treated oral mucosa2005

    • Author(s)
      Seiichiro FUJISAWA
    • Journal Title

      Dental Materials Journal 24・2

      Pages: 202-206

    • NAID

      110002983757

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Cytotoxicity and apoptosis-inducing activity of bisphenol A and hydroquinone in HL-60 cells2005

    • Author(s)
      Hiroshi TERASAKA
    • Journal Title

      Anticancer Research 25・3B

      Pages: 2241-2247

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A comparative study of the antioxidant/prooxidant activities of eugenol and isoeugenol with various concentrations and oxidation conditions2005

    • Author(s)
      Toshiko ATSUMI
    • Journal Title

      Toxicology in Vitro 19

      Pages: 1025-1033

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Induction of cytotoxicity and apoptosis and inhibition of cyclooxygenase-2 gene expression, by curcumin and its analog, α-diisoeugenol2005

    • Author(s)
      Toshiko ATSUMI
    • Journal Title

      Anticancer Research 25・6B

      Pages: 4029-4036

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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