• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of the Nod signals which enhance bone resorption

Research Project

Project/Area Number 17592119
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Surgical dentistry
Research InstitutionMatsumoto Dental University

Principal Investigator

YANG Shufa  Matsumoto Dental University, Faculty of Dentistry, Research Associate (80360220)

Co-Investigator(Kenkyū-buntansha) UEMATSU Takashi  Matsumoto Dental University, Graduate School for Dentistry, Associate Professor (40203476)
FURUSAWA Kiyofumi  Matsumoto Dental University, Graduate School for Dentistry, Professor (90165481)
TAKAHASHI Naoyuki  Matsumoto Dental University, Graduate School for Dentistry, Professor (90119222)
UDAGAWA Nobuyuki  Matsumoto Dental University, Graduate School for Dentistry, Professor (70245801)
TAKAHASHI Masahiro  Matsumoto Dental University, Faculty of Dentistry, Research Associate (90340059)
中道 裕子  松本歯科大学, 総合歯科医学研究所, 助手 (20350829)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥5,880,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsbone resorption / lipopolysaccharide / peptidoglycan / osteoclasts / RANKL / 骨芽細胞 / Nod2 / 炎症性サイトカイン / 歯周疾患
Research Abstract

Muramyl dipeptide (MDP) and DAP-containing desmuramyl peptide (iE-DAP) are the minimal essential structural units responsible for the immunoadjuvant activity of peptidoglycan. As well as bone-resorbing factors such as 1α, 25-dihydroxyvitamin D3(1α, 25(OH)2D3) and PGE2, LPS and IL-1 stimulate osteoclast formation in mouse cocultures of primary osteoblasts and hemopoietic cells. MDP or FK156 (artificial compound of iE-DAP) alone could not induce osteoclast formation in the coculture, but enhanced osteoclast formation induced by LPS, IL-1. FK156 enhanced osteoclast formation in the presence of low dose of 1α, 25(OH)2D3 or IL-1. MDP or FK156 failed to enhance osteoclast formation from osteoclast progenitors induced by receptor activator of NF-kB ligand(RANKL). MDP up-regulated RANKL expression in osteoblasts treated with LPS or TNF-α but not 1α, 25(OH)2D3. Osteoblasts expressed mRNA of nucleotide-binding oligomerization domain 2 (Nod2), an intracellular sensor of MDP, in response to LPS, IL-1, or INF-α but not 1α, 25(OH)2D3. The depletion of intracellular Nod2 by small interfering RNA blocked MDP-induced up-regulation of RANKL mRNA in osteoblasts. LPS and RANKL stimulated the survival of osteoclasts, and this effect was not enhanced by MDP. These results suggest that MDP or FK156 synergistically enhance osteoclast formation induced by LPS or IL-1through RANKL expression in osteoblasts, and that Nod2-mediated signals are involved in the MDP-induced RANKL expression in osteoblasts as well as Nod1 signaling.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (12 results)

All 2008 2007 2006

All Journal Article (11 results) (of which Peer Reviewed: 1 results) Book (1 results)

  • [Journal Article] Docetaxel inhibits bone resorption through suppression of osteoclast for mation and function in different manners2008

    • Author(s)
      Takahashi M, et. al.
    • Journal Title

      J Bone Miner Metab 27(In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Docetaxel inhibits bone resorption through suppression of osteoclast formation and function in different manners.2008

    • Author(s)
      Takahashi M, Takahashi M, Mizoguchi T, Uehara S, Nakamichi Y, Yang S, Naramoto H, Yamashita T, Kobayashi Y, Furusawa K, Yamaoka M, Udagawa N, Uematsu T, Takahashi N
    • Journal Title

      J Bone Miner Metab (in press)

    • NAID

      10025336084

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Signal transduction of lipopolysaccharide-induced osteoclast differentiation2007

    • Author(s)
      Udagawa N, et. al.
    • Journal Title

      Periodontology 2000 43

      Pages: 56-64

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Signal transduction of lipopolysaccharide-induced osteoclast differentiation.2007

    • Author(s)
      Udagawa N, Sato N, Yang S, Nakamura M, Yamashita T, Nakamura H, Noguchi T
    • Journal Title

      Periodontology 2000 43

      Pages: 56-64

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Signal transduction of lipopolysaccharide-induced osteoclast differentiation.2007

    • Author(s)
      Udagawa N, et. al.
    • Journal Title

      Periodontol 2000 43

      Pages: 56-64

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Establishment of primary cultures for mouse ameloblasts as a model of the life time.2006

    • Author(s)
      Suzawa T et al.
    • Journal Title

      Biochem Biophys Res Commun 345・(3)

      Pages: 1247-1253

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Gastric inhibitory polypeptide as an endogenous factor promoting new bone formation following food ingestion.2006

    • Author(s)
      Tsukiyama K et al.
    • Journal Title

      Mol Endocrinol 20・(7)

      Pages: 1644-1651

    • Related Report
      2006 Annual Research Report
  • [Journal Article] A critical role for interleukin-6 family-mediated Stat3 activation in osteoblast differentiation and bone formation.2006

    • Author(s)
      Itoh S et al.
    • Journal Title

      Bone 39・(3)

      Pages: 502-512

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Osteoblasts provide a suitable microenvironment for the action of receptor activator of NF-κB ligand.2006

    • Author(s)
      Yamamoto Y et al.
    • Journal Title

      Endocrinology 147・(7)

      Pages: 3366-3374

    • Related Report
      2006 Annual Research Report
  • [Journal Article] The mechanism of coupling between bone resorption and bone formation.2006

    • Author(s)
      Udagawa N et al.
    • Journal Title

      J Oral Biosciences 48・(3)

      Pages: 185-197

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Coordination of microtubules and the actin cytoskeleton is important in osteoclast function, but calcitonin selectively disrupts actin rings without affecting microtubule networks. Submitted for publication.2006

    • Author(s)
      Okumura S et al.
    • Journal Title

      Bone 39・(4)

      Pages: 684-693

    • Related Report
      2006 Annual Research Report
  • [Book] Arthritis Research, Methods and Protocols Volume 1 "Generation of osteoclasts in vitro, and assay of osteoclast activity"2006

    • Author(s)
      Takahashi N et al.
    • Publisher
      Humana Press
    • Related Report
      2006 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi