| Project/Area Number |
17592120
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Matsumoto Dental University |
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Principal Investigator |
LI Xianqi Matsumoto Dental University, Institute for Oral Science, Research Associate, 総合歯科医学研究所, 助手 (60350831)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUURA Takashi Matsumoto Dental University, Faculty of Dentistry, Lecturer, 歯学部, 講師 (10298408)
NARAMOTO Hiroko Matsumoto Dental University, Faculty of Dentistry, Research Associate, 歯学部, 助手 (30410426)
UEMATSU Takashi Matsumoto Dental University, Graduate School of Oral Medicine, Associate Professor, 総合歯科医学研究所, 助教授 (40203476)
OZAWA Hidehiro Matsumoto Dental University, Graduate School of Oral Medicine, Professor, 総合歯科医学研究所, 教授 (60018413)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | vaccine / OK-432 / cancer / tumor immunity / cytokine |
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| Research Abstract |
We have previously documented that OK-432-conjugated tumor vaccine (KLN-205 vaccine) strongly suppressed tumor incidence and growth, and improved survival rate as tumor-specific immunity in murine tongue cancer model. The aim of this study is to clarify the mechanisms of antitumor immune response elicited by KLN-205 vaccine. Six-week-old female DBA/2 mice were individually randomized, divided into three groups. KLN-205 tumor cells derived from the squamous cell carcinoma of DBA/2 mice. KLN-205 cells were mixed with OK-432 serviced as KLN-205 vaccine. The prepared KLN-205 vaccine was injected into mice once a week for 3 weeks, and splenetic cells from the mice were isolated and cultured with tumor cells. Cytokines in serum and culture supernatant were then measured using ELISA. Immunohistochemical analysis for CD4, CD8, CD25(IL-2Rα), CD69, and CD122(IL-2Rβ) was performed by indirect immunoperoxidase staining. When the mice were immunized with the KLN-205 vaccine three times, higher INF-y levels were seen in serum and culture supernatant of splenetic cells than the control mice. After tumor inoculation in the KLN-205 vaccine group, a greater number of CD4 and CD8 T cells had infiltrated around tumor cells than the control group. Moreover immunohistochemical results showed that the expression of CD25(IL-2Rα), CD69, and CD122(IL-2Rβ) in T cells were transiently higher by 24 hours in the KLN-205 vaccine group. These results indicated that the KLN-205 vaccine induced tumor-specific cell-mediated immunity and suppress tumor activity.
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