| Project/Area Number |
17592123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Kurume University |
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Principal Investigator |
IWAMOTO Osamu Kurume University, Lecture, 医学部, 講師 (80247527)
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| Co-Investigator(Kenkyū-buntansha) |
KUSUKAWA Jingo Kurume University, Professor, 医学部, 教授 (30258412)
KOGA Chihiro Kurume University, Associate Professor, 医学部, 助教授 (80258417)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2006: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2005: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | cancer / virus / immunology |
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| Research Abstract |
The summary of experimental results was as follows :
1. HSV-1 antigen expression in 102 oral squamous cell carcinoma (SCC) cases was immunohistochemically evaluated using anti-rabbit polyclonal antibody, and the positive rate was approximately 53% (HSV-1-positive group). Furthermore, the expression (330 bp) was confirmed at the gene level (RT-PCR method).
2. Retrospective comparison of HSV-1-positive and -negative groups showed that the rate of cervical lymph node metastasis was significantly lower in the former compared to the latter (p<0.01). Furthermore, the 5-year cumulative survival rate was higher in the positive group (95% vs. 83%, respectively).
3. Neutralizing antibody titers and serum IgG were significantly higher in the HSV-1-positive group (p<0.01).
4. The HSV-1-positive group showed marked infiltration of CD8-positive T-lymphocytes in the cancerous tissue. These results suggested that HSV-1 occurring in cancers increased immunological function around cancers, which inhibited lymph node metastasis. To elucidate the expression pathway of HSV-1 in oral cancers, we are producing an oral cancer model using HSV-1-latently infected Syrian hamsters.
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