| Project/Area Number |
17592124
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
KIM Takenori Hokkaido University, Graduate School of Dental Medicine, Instructor, 大学院歯学研究科, 助手 (20344517)
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| Co-Investigator(Kenkyū-buntansha) |
IIDA Junichiro Hokkaido University, Graduate School of Dental Medicine, Professor, 大学院歯学研究科, 教授 (90151232)
山岡 雄司 北海道大学, 大学院・歯学研究科, 助手 (50322821)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | PDL cells / Osteoclasts / Osteoimmunocyte / Continuous orthodontic force / RANKL / Cytokines / ラット歯列拡大装置 / 持続的機械的ストレス負荷モデル |
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| Research Abstract |
Objectives-This study investigated longitudinal changes in RANKL expression in PDL cells subjected to a continuous orthodontic force.
Design-Fifty-five-days-old male Wistar rats were divided into experimental and control groups. The experimental group had the first molars laterally expanded by a continuous orthodontic force. In each group, the horizontal section specimens were embedded in OTC compound and frozen at 0,1,3 and 7 days after the expansion. Sections were observed by immunostaining with anti-RANKL and the tartrate-resistant acid phosphatase (TRAP) staining. Result-Immunoreaction of RANKL and TRAP-positive cells were observed in the distal region of the controls and on the compressed side of the expansion group in the 3-and 7-days. Immunoreaction of RANKL was also observed after 1-day on the compression side of the expansion group, but here TRAP-positive cells were few.
Conclusions-The experiments have showed that PDL cells are continuously producing RANKL on the PDL pressure side of rats subjected to mechanical stress with a continuous orthodontic force, there was no noticeable the excessive appearance of osteoclasts however. Considering this, it is expected that not only RANKL production but also other cytokines play an important role in the balancing adjustment in the alveolar bone remodeling.
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