| Project/Area Number |
17592131
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
SUZUKI Shoichi Tokyo Medical and Dental University, Graduate School, Associate Professor, 大学院医歯学総合研究科, 准教授 (90187732)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Yoshiyuki Tokyo Medical and Dental University, Graduate School, Assistant Professor, 大学院医歯学総合研究科, 助教 (70251535)
KAWAMOTO Tatsuo Tokyo Medical and Dental University, Graduate School, Assistant Professor, 大学院医歯学総合研究科, 助教 (50323704)
SHIMOKAWA Hitoyata Tokyo Medical and Dental University, Graduate School, Associate Professor, 大学院医歯学総合研究科, 准教授 (80014257)
SHIBATA Shunichi Health Science University of Hokkaido, School of Dentistry, Professor, 歯学部, 教授 (80187400)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Mandibular condyle / IGF-I / apoptosis / IGFBP3 / caspase 3 / アンチセンス / IGF-IR / 下顎頭軟骨細胞 |
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| Research Abstract |
The purpose of this study was to examine the role of endogenous insulin-like growth factor I (IGF-I) in promoting survival of cells of the mandibular condyle in culture. Mandibular condyles dissected from 12-day-old rats were cultured for 1, 3 or 5 days in medium containing antisense oligodeoxynucleotide (AS-ODN) or sense-ODN (S-ODN) for IGF-I. Quantitative real-time RT-PCR analysis showed that the levels of IGF-I and IGF binding protein (IGFBP)3 mRNAs in tissues treated with AS-ODN were significantly decreased compared with control and S-ODN group. On days 3 and 5, the number of denatured cells was observed in the undifferentiated mesenchymal cell layer. These cells were TUNEL positive and confirmed to be apoptotic by electron microscopic observation. Immunoblotting revealed that expression of cleaved caspase3 was increased with AS-ODN. These results indicate that the cells in the undifferentiated mesenchymal cell layer of mandibular condyle require IGF-I for survival.
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