Experimental study on caries prevention by gene transfer of anti microbial peptides beta-defensins

• Project/Area Number: 17592146
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Health Sciences University of Hokkaido
• Principal Investigator: IGARASHI Seiji Health Sciences University of Hokkaido, 歯学部, 教授 (20001943)
• Co-Investigator(Kenkyū-buntansha): ABIKO Yoshihiro 北海道医療大学, 個体差医療科学センター, 教授 (90260819) ; SAITOH Masato 北海道医療大学, 個体差医療科学センター, 講師 (50337036)
• Project Period (FY): 2005 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,500,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥300,000); Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2007: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000); Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
• Keywords: βディフェンシン / 抗菌ペプチド / う蝕 / マウス唾液腺 / アデノウィルス / 培養角化細胞 / 歯肉溝滲出液 / 抗菌タンパク / アデノウイルス / siRNA / 遺伝子導入 / 細胞増殖 / 細胞分化

Research Abstract

βディフェンシンは口腔上皮での細菌防御機構に関与しており、特にβディフェンシン2が、う蝕の病原生菌の増殖を抑制するという報告がある。本研究ではβディフェンシン遺伝子導入による抗う蝕能を検討することを目的とした。研究結果から、βディフェンシン2とβディフェンシン3は唾液腺導管上皮の感染防御機構に関与していることが示唆された。またβディフェンシンは単に抗菌作用のみならず免疫系に関わっているが、本研究により細胞増殖や細胞分化にも関与することが示唆された。

Research Products

• [Journal Article] 歯肉溝滲出液における抗細菌性ペプチド・αディフェンシンの定量的評価 (2009)
  • Author(s): 佐藤惇、齊藤正人、畠山翔太、堀内美帆子、清水重善、倉重圭史、松坂賢一、井上孝、五十嵐清治、安彦善裕
  • Journal Title: 口腔検査誌 1 Pages: 24-27
  • Peer Reviewed
• [Journal Article] Profiling of differentially expressed genes in porcine epithelial cells derived from periodontal ligament and gingiva by DNA microarray (2008)
  • Author(s): Kurashige Y, Saitoh M, Nishimura M, NoroD, Kaku T, Igarashi S, Takuma T, Arakawa T, Inoue T, Abiko Y
  • Journal Title: Arch Oral Biol 53 Pages: 437-442
  • Peer Reviewed
• [Journal Article] Profiling of differentially expressed genes in porcine epithelial cells derived from periodontal ligament and gingiva by DNA microarray. (2008)
  • Author(s): Kurashige Y, Saitoh M, Abiko Y, Igarasgi S, et al.
  • Journal Title: Arch Oral Biol 53 Pages: 437-442
  • Peer Reviewed
• [Journal Article] High frequency of hypermethylation of p14, p15 and p16 in oral pre-cancerous lesions associated with betel-quid chewing in Sri Lanka. (2008)
  • Author(s): Takeshima M, Saitoh M, Abiko Y, et al.
  • Journal Title: J Oral Pathol Med 37 Pages: 475-479
  • Peer Reviewed
• [Journal Article] 歯肉溝滲出液における抗細菌性ペプチド・αディフェンシンの定量的評価 (2008)
  • Author(s): 佐藤惇, 齊藤正人, 安彦善裕, 五十嵐清治, 他
  • Journal Title: 日本口腔検査学会誌 1 Pages: 24-27
  • Peer Reviewed
• [Journal Article] Decreased expression of beta defensin-1,-2,-3 in HaCaT cells induces their proliferation followed by the alteration of their differentiation (2008)
  • Author(s): Masato Saitoh, Seiji Igarashi, Yoshihiro Abiko, et. al.
  • Journal Title: Journal of Dermatological Science (In press)
  • Peer Reviewed
• [Journal Article] Increased expression of beta-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL (2007)
  • Author(s): Saitoh M,Kurashige Y, Yamazaki M, Nishimura M, Nakamura S, Noro D, Takeshima M, Arakawa T, Takuma T, Igarashi S, Kaku T, Inoue T, Abiko Y
  • Journal Title: lpr mice. Med Mol Morphol 40 Pages: 157-162
  • Peer Reviewed
• [Journal Article] Salivary defensins and their importance in oral health and disease (2007)
  • Author(s): Abiko Y, Saitoh M
  • Journal Title: Curr Pharm Des 13 Pages: 3065-3072
  • Peer Reviewed
• [Journal Article] Role of beta-defensins in oral epithelial health and disease (2007)
  • Author(s): Abiko Y, Saitoh M, Nishimura M, Yamazaki M, Sawamura D, Kaku T
  • Journal Title: Med Mol Morphol 40 Pages: 179-184
  • Peer Reviewed
• [Journal Article] Increased expression of beta-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL/lpr mice (2007)
  • Author(s): Masato Saitoh, Seiji Igarashi, Yoshihiro Abiko, et. al.
  • Journal Title: Medical Molecular Morphology 40・3 Pages: 157-162
  • Peer Reviewed
• [Journal Article] Salivary defensins and their importance in oral health and disease (2007)
  • Author(s): Yoshihiro Abiko, Masato Saitoh
  • Journal Title: Current Pharmaceutical Design 13・30 Pages: 3065-3072
  • Peer Reviewed
• [Journal Article] Role of beta-defensins in oral epithelial health and disease (2007)
  • Author(s): Yoshihiro Abiko, Masato Saitoh, et. al.
  • Journal Title: Medical Molecular Morphology 40・4 Pages: 179-184
  • Peer Reviewed
• [Journal Article] Increased expression of beta-defensin-2 and -3 during the development of autoimmune sialoadenitis in MRL/lpr mice (2007)
  • Author(s): Masato Saitoh, Seiji Igarashi, Yoshihiro Abiko et al.
  • Journal Title: Medical Molecular Morphology (In press)
• [Journal Article] Salivary defensins and their importance in oral health and disease (2007)
  • Author(s): Yoshihiro Abiko, Masato Saitoh
  • Journal Title: Current Pharmaceutical Design (In press)
• [Journal Article] 唾液と歯肉のディフェンシン -その臨床的意義- (2005)
  • Author(s): 安彦善裕
  • Journal Title: 日本歯科評論 増刊2005 Pages: 179-184
• [Presentation] Decreased Expression of Beta-defensins Alters Keratinocyte Proliferation and Differentiation (2008)
  • Author(s): Saitoh M, Igarashi S, Abiko Y, et al.
  • Organizer: IADR
  • Place of Presentation: Tronto, Canada
  • Year and Date: 2008-07-04
• [Presentation] Decreased expression of beta-defensins alters keratinocyte proliferation and differentiation (2008)
  • Author(s): Saitoh M, Yamazaki M, Nakamura S, Horiuchi M, Kurashige Y, Noro D, Nishimura M. Igarashi S, Abiko Y
  • Organizer: 86^<th> International Association for Dental Research
• [Presentation] Effect of corticosteroid on beta-defensins expression stimulated with TLR agonists (2008)
  • Author(s): Horiuchi M, Saitoh M, Nakamura S, Nishimura M, Yamazaki M, Kurashige Y, Igarashi S, Abiko Y
  • Organizer: 86^<th> International Association for Dental Research
• [Presentation] Corticosteroid affects upregulated expression of beta-defensins in keratinocytes (2008)
  • Author(s): Horiuchi M, Saitoh M, Nakamura S, Nishimura M, Yamazaki M, Kurashige Y. Kaku T, Igarashi S, Abiko Y
  • Organizer: 56^<th> Annual Meeting of Japanese Association for Dental Research
• [Presentation] Expression of β-defensins in autoimmune sialoadenitis of MRL/lpr mice (2007)
  • Author(s): Saitoh M. Yamazaki M. Kurashige Y, Takeshima M, Nakamura S, Igarashi S, Noro D, Abiko Y
  • Organizer: 85^<th> International Association for Dental Research
• [Presentation] Malassez epithelial cells inhibit osteoblastic mineralization in co-culture systems (2007)
  • Author(s): Noro D, Saitoh M, Yamazaki M, Nishimura M, Horiuchi M, Igarashi S, Abiko Y
  • Organizer: 8^<th>h International Association for Dental Research.
• [Presentation] Increased expression of β-defensins during of sialoadenitis in MRL/lpr-mice (2007)
  • Author(s): Saitoh M, Kurashige Y, Noro D, Yamazaki M, Nakamura S, Takeshima M, Horiuchi M, Igarashi S, Abiko Y
  • Organizer: 21st International Association for Dental Research - South East Asia Division
• [Presentation] Malassez-epithelial cells promote osteoblastic cell proliferation and migration in co-culture (2007)
  • Author(s): Noro D, Saitoh M, Yamazaki M, Nishimura M, Horiuchi M, Igarashi S, Abiko Y
  • Organizer: 21st International Association for Dental Research - South East Asia Division
• [Presentation] Expression of keratinocyte differentiation markers accompanied by alteration of beta-defensins (2006)
  • Author(s): Saitoh M, Yamazaki M, Nishimura M, Shimizu S, Noro D, Igarashi S, Abiko Y
  • Organizer: 84^<th> International Association for Dental Research
• [Presentation] Effect of Malassez-epithelial cells on osteoblast cells in co-culture system (2006)
  • Author(s): Noro D, Saitoh M, Igarashi S, Nishimura M, Yamazaki M, Abiko Y
  • Organizer: 84^<th> International Association for Dental Research
• [Book] 世代をつなぐ小児歯科 (2009)
  • Author(s): 五十嵐清治, 齊藤正人, 他
  • Total Pages: 192
  • Publisher: クインテッセンス出版