Basic study on the effect of mastication for growth and development of dentition
| Project/Area Number |
17592152
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
KIMOTO Shigenari Kanagawa Dental College, Dentistry, Professor (90205013)
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| Co-Investigator(Kenkyū-buntansha) |
KAWASE Toshio Kanagawa Dental College, Dentistry, Professor (30084784)
MATSUZAWA Mitsuhiro Kanagawa Dental College, Dentistry, Assistant professor (60288082)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,300,000 (Direct Cost: ¥1,300,000)
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| Keywords | Dentistry / Osteoblast / Differentiation / Periodontal Tissue |
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| Research Abstract |
It is not clear the effect of TRAP on the cells derived from periodontal ligament that have osteoblast like characters under the condition with mechanical stress. We used the recombinant protein of TRAP(NOVUS Co. Ltd.) in this study. Osteoblasts were cultured in the stretch chambers(STREX Co. Ltd.) and loaded with cyclic mechanical stretching by cell stretch system(SIB-140). The cells were cultured for 72 hours to preconfluent phase with or without TRAP in the culture medium then stretched with 20% elongation horizontally at 40 cycles per minutes for 16 hours. The results from Real-time PCR revealed that TRAP addition decreased the mRNA level of Osterix and CBFA1 on the MC3T3 cells furthermore loading with mechanical stretch enhanced the effects. We investigated the effect of mechanical stress on mRNA expression level of RANKL and OPG. TRAP or mechanical stretching did not affect the expression level of OPG mRNA. On the other hand RANKL mRNA level increased by TRAP addition while mechanical stretching decreased the mRNA level. Mechanism of the suppression on osteoblast differentiation by TRAP and mechanical stretching. Was not clear however the effect of mechanical stretching suggested the osteoblasts in alveolar bone suppress osteoclast differentiation during tooth movement.
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Report
(4 results)
Research Products
(53 results)
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[Journal Article] Proliferation and differentiation of rat bone marrow stromal cells on poly(glycolicacid)-collagen sponge2005
Author(s)
Fujita M, Kinoshita Y, Sato M, Maeda H, Ozono S, Negishi H, Kawase T, Hiraoka Y, Takamoto T, Tabata Y, Kameyana Y
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Journal Title
Tissue Engineering 11
Pages: 1346-1355
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Proliferation and differentiation of rat bone marrow stromal cells on poly(glycolic acid)-collagen sponge2005
Author(s)
Fujita M, Kinoshita Y, Sato M, Maeda H, Ozono S, Negishi H, Kawase T, Hiraoka Y, Takamoto T, Tabata Y, Kameyana Y
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Journal Title
TissueEngineering vol.11
Pages: 1346-1355
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] 多層細胞培養による歯槽骨移植片作製の試み2005
Author(s)
尾形 顕, 辻上 弘, 杉本 久, 小田原 謙介, 新田 稔, 根本 賢治, 瑞穂 圭, 宮定 健市, 菅谷 彰, 川瀬 俊夫, 出口 眞二
Organizer
第40回神奈川歯科大学学会・総会
Place of Presentation
横須賀
Year and Date
2005-12-03
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] ヒト骨髄由来細胞を用いたスフェロイド(細胞凝集塊)の歯周組織再生応用への免疫組織化学的研究2005
Author(s)
森本 孝之, 尾形 顕, 辻上 弘, 杉本 久, 光家 由紀子, 前堀 隆光, 田胡 和浩, 堀俊 太郎, 井手 口隆, 菅谷 彰, 出口 眞二, 川瀬 俊夫
Organizer
第40回神奈川歯科大学学会・総会
Place of Presentation
横須賀
Year and Date
2005-12-03
Description
「研究成果報告書概要(和文)」より
Related Report
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[Presentation] Trial for preparation of the transplant for human alveolar bone by multilayer cell culture2005
Author(s)
Ogata A, Tsujigami H, Sugimoto H, Odawara K, Nitta M, Nemoto K, Mizuho K, Miyasada K, Sugaya A, Kawase T, Deguchi S
Organizer
40th General Meeting of Kanagawa Odontological Society
Place of Presentation
Yokosuka
Year and Date
2005-12-03
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Immunohistochemical study for application of the spheroid of human bone marrow cells to regeneration of periodontal tissue2005
Author(s)
Morimoto T, Ogata A, Tsujigami H, Sugimoto H, Kouke Y, Maehori T, Tago K, Hon S, Ideguchi T, Sugaya A, Deguchi S, Kawase T
Organizer
40th General Meeting of Kanagawa Odontological Society
Place of Presentation
Yokosuka
Year and Date
2005-12-03
Description
「研究成果報告書概要(欧文)」より
Related Report
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