| Co-Investigator(Kenkyū-buntansha) |
TATSUMI Junichi Meikai University, School of Dentistry, Associate Professor (60227105)
HAYASHI Joichiro Miekai University, School of Dentistry, Associate Professor (50337507)
OTSUKA Hideharu Meikai University, School of Dentistry, Assistant Professor (10271230)
TAKEDA Hiroyuki Meikai University, School of Dentistry, Assistant Professor (40316691)
OHASHI Toshio Meikai University, School of Dentistry, Assistant Professor (40337506)
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| Budget Amount *help |
¥3,760,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥900,000 (Direct Cost: ¥900,000)
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| Research Abstract |
Platelet-rich plasma (PRP) prepared from peripheral blood contain various growth factors. Therefore, PRP has been used to promote wound healing and bone regeneration. However, the effect of PRP on periodontal tissue regeneration was unclear. The aim of this study was to devise a new protocol for PRP preparation without the use of exogenous thrombin, and to clarify the effect of PRP on periodontal regeneration in vivo. And further more, we investigate the regenerative effect of periodontal tissue by highly concentrated platelet-rich plasma in vivo.
Peripheral blood was drawn from 21 human volunteers, and PRP was prepared by using the double-spin method with a standard laboratory centrifuge. Levels of growth factors determine quantity by ELISA Kit and P-selectin was measured by EIA Kit. For the in vivo study to evaluate the regenerative effects of PRP implanted into a bone defect, 2-wall bony defects were made on the bony wall surrounding a mandibular premolar. PRP (PRP group) and highly-
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concentrated PRP(h-PRP group)was applied to one defect, and the other was left untreated (control group). The animals were euthanized at 4, 8, and 12 weeks postoperatively; and serial sections were prepared and stained with H-E or immunostained with osteocalcin monoclonal antibody. Newly formed bone, cementum height, and epithelial downgrowth were measured by histomorphometry and localization of osteocalcin was examined.
Each growth factor was increased to 2.5 to 4.2 times its level in the peripheral blood (PDGF-AA, 4.2 times; PDGF-BB, 3.6; PDGF-AB, 4.2; TGF-β_1, 3.7; TGF-β_2, 3.2; VEGF, 3.3; and IGF-, 2.5). P-selectin levels in the PRP were not significant compared with those in the peripheral blood. PRP groups showed enhanced formation of new cementum formation in vivo at 4 and 8 weeks and an increased number of blood vessels at 8 weeks. Epithelial down growth was inhibited at 8 and 12 weeks in the PRP groups. Furthermore, expression of osteocalcin showed strong localization in the root surface of the notch area in PRP groups. However there was no significant difference in the rate of new bone formation between the PRP, h-PRP and control groups. Less
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