| Project/Area Number |
17592171
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Periodontal dentistry
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| Research Institution | Kanagawa Dental College |
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Principal Investigator |
DEGUCHI Shinji Kanagawa Dental College, Dentistry, Professor (60121018)
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| Co-Investigator(Kenkyū-buntansha) |
SUGAYA Akira Kanagawa Dental College, Dentistry, Associate Professor (30162853)
TAGO Kazuhiro Kanagawa Dental College, Dentistry, Assistant Professor (00386830)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,140,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | human alveolar bone periosteal cells(HABPCs) / regeneration / graft / immunohistochemical staining |
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| Research Abstract |
We studied the possibility of using grafts composed of human alveolar bone periosteal cells (HABPCs) for periodontal regeneration. The periodontium was obtained during periodontal partial thickness flap surgery. Written informed consent was obtained from those who agreed to participate voluntarily and ethical clearance was obtained from the institution's ethical committee. We produced cell sheets using HABPCs with containing 10 % fetal calf serum medium (α-MEM). HABPCs was inoculated four times to become the multilayer cell sheet every week that was cultured for three days after folded to create the graft. The graft morphology and protein expression were analyzed using histological (hematoxylin and eosin, alkaline phosphatase (ALP) and von Kossa's staining) and immunohistochemical (type I collagen (Col-I), osteopontin (OPN), osteocalcin (OC) and Ki-67) studies on the graft sections.
The graft be able to be used with tweezers easily, must be about 5 X 10 mm in size, and must be about 1.5 mm thickness. As a result of the histological observation, the graft was consisted only of the cell and the extracellular matrix. Immunohistochemical staining of the graft sections showed a strong positive reaction for Col-I, ALP and included OC, OPN and Ki-67 positive cell.
It was suggested that graft might be calcified by the above-mentioned results. Therefore, it was thought that the graft could be applied to an intraosseous defect for periodontal regeneration.
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