| Project/Area Number |
17592173
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
TAKAHASHI Dairo Hokkaido University, Graduate School of Dental Medicine, Assisitant Professor (80312370)
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| Co-Investigator(Kenkyū-buntansha) |
MORITA Manabu Hokkaido University, Graduate School of Dental Medicine, Professor (40157904)
KANDEHIRA Takashi Hokkaido University, University Hospital, Lecturer (90194935)
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| Project Period (FY) |
2005 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,740,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2005: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | diabetes mellitus / tooth-brushing / experimental periodontitis / gtingival massage / 歯肉マッサージ効果 |
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| Research Abstract |
The purpose of the present study was to determine an optimal tooth-brushing force for gingival massage in streptozotocin (STZ)-induced diabetic rats with experimental periodontitis
Forty-one male blister rats with STZ-induced diabetes mellitus (STZ-rat) were involved in the experiment. Under general anesthesia, lipopolysaccaride solution (25 μ g/ml) was applied once a day the palatal gingival sulcus of the maxillary first and second molars of STZ-rat to induce peridontitis for 8 weeks. From 5th to 8th week, the control group was given daily plaque removal using a hand instrument and the three experimental groups were given daily professional tooth-brushing with force of 100g (100G), 200g (200G), and 250g (250G) respectively The duration of tooth brushing was set for 20 seconds. The stress gauze was used for controlling the tooth brushing force. After 8 weeks, the STZ-rats sacrificed. The teeth and gingivae were fixed, decalcified and embedded in paraffin. Buccolingual sections (5μm) were used for histologically observation and histometrically evaluation.
The blood glucose level of STZ-rats assessed by glucose debydrogenase method was about 450 mg/dl through experimental duration. Histological observation found similar results with respect to gingival inflammation and epithelial downgrowth. Strongly gingival inflammation and resorption of alveolar bone was partially observed for control, 200G, 250G rats. On the other hand, no marked epithelial downgrowth and severe resorption of alveolar bone was observed for 100g rats. In addition, histometrically evaluation indicated that a degree of epithelial downgrowth and resorption of alveolar bone was low in the 100G group compare with in the another groups.
The results of this study indicate that tooth-brushing with force of 100g was available for gingival massage in diabetic status.
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