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Experimental aspiration pneumonia induced by human supragingival dental plaque in mice

Research Project

Project/Area Number 17592185
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Social dentistry
Research InstitutionKagoshima University

Principal Investigator

NAGATA Emi  Kagoshima University, Medical and Dental Hospital, Research Associate, 医学部・歯学部附属病院, 助手 (00304816)

Co-Investigator(Kenkyū-buntansha) KITADA Katsuhiro  Kagoshima University, Graduate School of Medical and Dental Sciences, Research Associate, 大学院医歯学総合研究科, 助手 (90195264)
OHO Takahiko  Kagoshima University, Graduate School of Medical and Dental Sciences, Professor, 大学院医歯学総合研究科, 教授 (50160940)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordsdental plaque / aspiration pneumonia / oral bacteria / mouse / ラット
Research Abstract

Human dental plaque consists of a wide variety of microorganisms, some of which are believed to cause systemic infections, including aspiration pneumonia. To confirm this hypothesis experimentally, we examined the pneumonia-inducing ability of dental plaque in mice, the microbial features of the infectious locus produced by the plaque. Aliquots of a suspension of supragingival dental plaque containing 1 x 10^7 CFU of bacteria were delivered into the lower part of the trachea of mice. All mice exhibited signs of illness immediately after inoculation such as ruffled fur, labored respiration, and appeared waste. In total, 12 out of 18 mice (66.7%) died within 3 days after inoculation with dental plaque suspension. All the 3 human dental plaque samples tested induced pneumonia in mice. Bacterial cells were recovered from the lungs of all the mice that died, and the number was 4.44 ± 0.77 log_<10>CFU/mg of lung. From 5 out of 6 survived mice, bacterial cells were recovered from the lungs and the number was 0.38±0.38 log_<10>CFU/mg of lung. Various microbial species were recovered from infected lungs examined, and no characteristic bacterial distribution was observed both in the survived mice and the mice that died within 3 days after plaque inoculation. Representative isolates of Streptococcus oralis, Streptococcus intermedius and Gemella morbillorum recovered from lungs showed ability to induce pneumonia, respectively. These results suggest that human supragingival dental plaque is a source of the infectious pathogens that cause pneumonia.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (11 results)

All 2007 2006 2005

All Journal Article (11 results)

  • [Journal Article] Antibacterial activity of sodium fluoride against the Streptococcus anginosus group and Abiotrophia and Granulicatella species.2007

    • Author(s)
      Fukushima R., Nagata E., Inoue M., Oho T.
    • Journal Title

      Dentistry in Japan 43

      Pages: 95-98

    • NAID

      10021953474

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Serotype-specific polysaccharide of Streptococcus mutans contributes to infectivity in endocarditis.2006

    • Author(s)
      Nagata E., Okayama H., Ito H-O., Yamashita Y., Inoue M., Oho T.
    • Journal Title

      Oral Microbiol. Immunol. 21

      Pages: 420-423

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 緑茶抽出物のStreptococcus anginosus groupに対する抗菌作用2006

    • Author(s)
      北田勝浩, 井上昌一, 於保孝彦
    • Journal Title

      日本口腔衛生学会雑誌 56

      Pages: 132-136

    • NAID

      110004729662

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Antibacterial activity of green tea extracts against Streptococcus anginosus group.2006

    • Author(s)
      Kitada K., Inoue M., Oho T.
    • Journal Title

      J. Dent. Hlth. 56

      Pages: 132-136

    • NAID

      110004729662

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Serotype-specific polysaccharide of Streptococcus mutans contributes to infectivity in endocarditis.2006

    • Author(s)
      Nagata E., Okayama H., Ito H-O., Yamashita Y., Inoue M., Oho T.
    • Journal Title

      Oeal Microbiol. Immunol. 21

      Pages: 420-423

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Experimental abscess formation caused by human dental plaque.2005

    • Author(s)
      Okayama H., Nagata E., Ito H-O., Oho T., Inoue M.
    • Journal Title

      Microbiol. Immunol. 49・5

      Pages: 399-405

    • NAID

      10015708099

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Experimental infective endocarditis induced by human supragingival dental plaque in rats.2005

    • Author(s)
      Nagata E., Okayama H., Ito H-O., Semba I., Inoue M., Oho T.
    • Journal Title

      Eur. J. Oral Sci. 113・6

      Pages: 499-504

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Experimental abscess formation caused by human dental plaque.2005

    • Author(s)
      Okayama H., Nagata E., Ito H-O., Oho T., Inoue M.
    • Journal Title

      Microbiol. Immunol. 49

      Pages: 399-405

    • NAID

      10015708099

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Experimental infective endocarditis induced by human supragingival dental plaque in rats.2005

    • Author(s)
      Nagata E., Okayama H., Ito H-O., Semba I., Inoue M, Oho T.
    • Journal Title

      Eur. J. Oral Sci. 113

      Pages: 499-504

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Experimental abscess formation caused by human dental plaque2005

    • Author(s)
      Okayama H., Nagata E., Ito H-O., Oho T., Inoue M.
    • Journal Title

      Microbiol.Immunol. 49・5

      Pages: 399-405

    • NAID

      10015708099

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Experimental infective endocarditis induced by human supragingival dental plaque in rats2005

    • Author(s)
      Nagata E., Okayama H., Ito H-O., Semba I., Inoue M., Oho T.
    • Journal Title

      Eur.J.Oral Sci. 113・6

      Pages: 499-504

    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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