Binding Affinity of Actinobacillus actinomycetemcomitans for extracellular matrix proteins
| Project/Area Number |
17592190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Social dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
KAWATO Takayuki Nihon University, School of Dentistry, Assistant, 歯学部, 助手 (50386075)
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| Co-Investigator(Kenkyū-buntansha) |
MAENO Masao Nihon University, School of Dentistry, Professor, 歯学部, 教授 (60147618)
MOTOHASHI Masafumi Nihon University, School of Dentistry, Assistant Professor, 歯学部, 助教授 (90102615)
SHOJI Maiko Nihon University, School of Dentistry, Assistant, 歯学部, 歯学部助手 (30386099)
YAMASHITA Yoshihisa Kyushu University, School of Dentistry, Professor, 歯学部, 教授 (20192403)
関 みつ子 日本大学, 歯学部, 助手 (20226640)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2006: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
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| Keywords | A. actinomycetemcomitans / extracellular matrix protein / adhesion / collagen / fibronectin / mutans streptococci / GTF-I / cell surface protein antigen / A.actinomycetemcomitans / GTF-I / Actinobacillus actinomycetemcomitans / ラミニン / 歯周組織 |
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| Research Abstract |
Actinobacillus actinomycetemcomitans is an aetiologic agent in the development of periodontal and some systemic diseases in humans. In this study, the binding affinity of A. actinomycetemcomitans to 4 kinds of extracellular matrix protein (collagen type I, collagen type IV, fibronectin, and laminin) was investigated. A. actinomycetemcomitansY4 and IDH781 bound to immobilized fibronectin and collagen type IV more than collagen type I and laminin. The binding of the cells to immobilized fibronectin and type IV collagen was inhibited by both of soluble fibronectin and collagen type N. The soluble fibronectin, but not the soluble collagen type IV bound to the 40-kDa proteins that were extracted from Y4 and IDH781 cells. These results suggest that Actinobacillus actinomycetemcomitansY4 and IDH781 have high binding affinity for fibronectin and this binding may be regulated with the 40-kDa bacterial cell proteins. Y4 and IDH781 also showed high affinity for collagen type IV and the non-protein component of bacterial cells may be involved in binding to collagen type IV.
The cell surface protein antigen (PAg) and glucosyltransferases (GTFs) produced by Streptococcus sobrinus are major colonization factors of the organism, and the inhibition of these two factors should provide protection against dental caries. In this study, we constructed a fusion protein (PAgA-GB) composed of the alanine-rich region of PAg and the glucan-binding domain of GTF, and the antibodies against the fusion protein were prepared in rabbits. The antibody against PAgA-GB significantly inhibited the sucrose-dependent adhesion, but not sucrose-independent adhesion. These results show that the antibody against GTF-I may be useful for effective inhibition of the sucrose-dependent adhesion of S. sobrinus. However, PAg of S sobrinus may not function primarily as a receptor for acquired pellicles, and other cell surface proteins may be involved in the sucrose-independent adhesion of S sobrinus.
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Report
(3 results)
Research Products
(2 results)
