• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Involvement of guanylyl cyclase signals of dendritic cells in allergic state

Research Project

Project/Area Number 17607006
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field アレルギー
Research InstitutionKyoto Unversity

Principal Investigator

HORI Toshiyuki  Kyoto Unv, Grad. Sch. Med., Lecturer, 医学研究科, 講師 (70243102)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2005: ¥2,500,000 (Direct Cost: ¥2,500,000)
KeywordsANP / NO / guanylyl cyclase / dendritic cell / allergy / gualnylyl cyclase
Research Abstract

We investigated the immune regulatory mechanism via two kinds of guanylyl cyclases expressed in dendritic cells (DCs), GC-A that is the receptor for ANP and soluble GC that is the receptor for NO. First we examined the effects of NO on human DCs and found that NO polarizes both CD11c+ myeloid DCs and Plasmacytoid-plasmacytoid DCs (pDCs) toward a Th2-inducing phenotypes. Namely, IL-12 production of myeloid DCs on LPS stimulation as well as IFN-a production of pDCs on CpG oligo DNA stimulation was decreased by the addition of NO donor and these cells induced differentiation of umbilical cord-derived naive T cells into Th2 cells (J. Immunol. 175:806-812, 2005). Next, we analyzed GC-A+ cells in therapeutically resected tonsils by confocal microscopy and found that GC-A+ cells were present in the interfollicular T cell areas. Furthermore, flow cytometric analysis revealed that CD123+ cells that seemed to represent pDCs expressed high levels of GC-A. Fresh peripheral blood pDCs did not express GC-A but they were induced to express it after a short term culture with IL-3 or CpG oligo DNA. Addition of ANP induced an increase in intracellular cGMP levels and down-regulated production of IFN-a upon CpG oligo DNA stimulation, indicating that GC-A on pDCs was functional. These results suggest that pDCs in lymphoid organs express GC-A and are subjected to ANP-mediated immune regulation. Our results demonstrated that two kinds of guanylyl cyclases of DCs are deeply involved in the pathogenesis of Th2-dominant allergic state.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (20 results)

All 2007 2006 2005 Other

All Journal Article (20 results)

  • [Journal Article] Involvement of OX40 ligand+ mast cells in chronic GVHD after allogeneic hematopoietic stem cell transplantation.2007

    • Author(s)
      Ai Kotani, Toshiyuki Hori, Tomoko Fujita, Naotomo Kambe, et al.
    • Journal Title

      Bone Marrow Transpl. 39 (6)

      Pages: 373-375

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Involvement of 0X40 ligand+ mast cells in chronic GVHD after allogeneic hematopoietic stem cell transplantation.2007

    • Author(s)
      Ai Kotani, Toshiyuki Hori, Tomoko Fujita, Naotomo Kambe, Yumi Matsumura., Takayuki Ishikawa., Yoshiki Miyachi, Kenichi Nagai, Yuetsu Tanaka, Takashi Uchiyama.
    • Journal Title

      Bone Marrow Transpl. 39 (6)

      Pages: 373-375

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Roles of OX40 in the pathogenesis and control of diseases.2006

    • Author(s)
      Toshiyuki Hori
    • Journal Title

      Int. J. Hematol. 83 (1)

      Pages: 17-22

    • NAID

      10017147715

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Annual Research Report 2006 Final Research Report Summary
  • [Journal Article] Type I interferons attenuate T cell activating functions of human mast cells by decreasing TNF-α production and OX40 ligand expression while increasing IL-10 production.2006

    • Author(s)
      Tomoko Fujita, Naotomo Kambe, Takashi Uchiyama, Toshiyuki Hori
    • Journal Title

      J. Clin. Immunol. 26 (6)

      Pages: 512-518

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] IgE-activated mast cells in combination with pro-inflammatory factors induce Th2-promoting dendritic cells.2006

    • Author(s)
      Toshio Kitawaki, Norimitsu Kadowaki, Naoshi Sugimoto, Naotomo Kambe, Toshiyuki Hori, et al.
    • Journal Title

      Int. Immunol. 18 (12)

      Pages: 1789-1799

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Roles of OX40 in the pathogenesis and control of diseases.2006

    • Author(s)
      Toshiyuki Hori.
    • Journal Title

      Int. J. Hematol. 83 (1)

      Pages: 17-22

    • NAID

      10017147715

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Type I interferons attenuate T cell activating functions of human mast cells by decreasing TNF-α production and OX40 ligand expression while increasing IL-10 production.2006

    • Author(s)
      Tomoko Fujita, Naotomo Kambe, Takashi Uchiyama, Toshiyuki Hori.
    • Journal Title

      J. Clin. Immunol. 26 (6)

      Pages: 512-518

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Functional characterization of 0X40 expressed on human CD8+ T cells.2006

    • Author(s)
      Tomoko Fujita, Naoya Ukyo, Toshiyuki Hori, Takashi Uchiyama
    • Journal Title

      mmunol. Lett. 106 (1)

      Pages: 27-33

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Type I interferons attenuate T cell activating functions of human mast cells by decreasing TNF-alpha production and 0X40 ligand expression while increasing IL-10 production.2006

    • Author(s)
      Tomoko Fujita, Naotomo Kambe, Takashi Uchiyama, Toshiyuki Hori
    • Journal Title

      J. Clin. Immunol. 26 (6)

      Pages: 512-518

    • Related Report
      2006 Annual Research Report
  • [Journal Article] IgE-activated mast cells in combination with pro-inflammatory factors induce Th2-promoting dendritic cells.2006

    • Author(s)
      Toshio Kitawaki, Norimitsu Kadowaki, Naoshi Sugimoto, Naotomo Kambe, Toshiyuki Hori, Yoshiki Miyachi, Tatsutoshi Nakahata, Takashi Uchiyama
    • Journal Title

      Int. Immunol. 18 (12)

      Pages: 1789-1799

    • Related Report
      2006 Annual Research Report
  • [Journal Article] TSLP-activated dendritic cells induce inflammatory TH2 response through OX40-ligand : a master switch of interleukin-10 and tumor necrosis factor-α.2005

    • Author(s)
      Tomoki Ito, Yui-Hsi Wang, Omar Duramad, Toshiyuki Hori, et al.
    • Journal Title

      J. Exp. Med. 202 (9)

      Pages: 1213-1223

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Nitric oxide inhibits IFN-α production of human plasmacytoid dendritic cells partly via a guanosine 3',5'-cyclic monophosphate-dependent pathway2005

    • Author(s)
      Rimpei Morita, Takashi Uchiyama, Toshiyuki Hori.
    • Journal Title

      J. Immunol. 175 (2)

      Pages: 806-812

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] TSLP-activated dendritic cells induce inflammatory TH2 response through OX40-ligand : a master switch of interleukin-10 and tumor necrosis factor-a.2005

    • Author(s)
      Tomoki Ito, Yui-Hsi Wang, Omar Duramad, Toshiyuki Hori, et al.
    • Journal Title

      J. Exp. Med. 202 (9)

      Pages: 1213-1223

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Phenotypic an functional relationship-between adult T cell leukemia cells and regulatory T cells.2005

    • Author(s)
      Yasushi Matsubara, Toshiyuki Hori, Rimpei Morita, Shimon Sakaguchi, Takashi Uchiyama
    • Journal Title

      Leukemia 19

      Pages: 482-483

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Introduction of OX40 ligand into lymphoma cells elicits anti-lymphoma immunity in vivo.2005

    • Author(s)
      Hitomi Kaneko, Toshiyuki Hori, Soshi Yanagita, Norimitsu Kadowaki, Takashi Uchiyama
    • Journal Title

      Exp.Hematol. 33

      Pages: 336-343

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nitric oxide inhibits IFN-α production of human plasmacytoid dendritic cells partly via a guanosine 3',5'-cyclic monophosphate-dependent pathway.2005

    • Author(s)
      Rimpei Morita, Takashi Uchiyama, Toshiyuki Hori
    • Journal Title

      J.Immunol. 175

      Pages: 806-812

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Endothelial cell co-stimulation through OX40 augments and prolongs T cell cytokine synthesis by stabilization of cytokine mRNA2005

    • Author(s)
      Javier Mestas, Steve P.Crampton, Toshiyuki Hori, Christopher C.W.Hughes
    • Journal Title

      Int.Immunol. 17

      Pages: 737-747

    • NAID

      10019358666

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Serological identification of adult T-cell leukaemia(ATL)-associated antigens.2005

    • Author(s)
      Masakatsu-Hishizawa, Kazunori Imada, Tomomi Sakai, Maki Ueda, Toshiyuki Hori, Takashi Uchiyama
    • Journal Title

      Br.J.Haematol. 130

      Pages: 382-390

    • Related Report
      2005 Annual Research Report
  • [Journal Article] TSLP-activated dendritic cells induce inflammatory response through OX40-ligand : a master switch of interleukin-10 and tumor necrosis factor-α2005

    • Author(s)
      Tomoki Ito, Yui-Hsri Wang, Omar Duramad, Toshiyuki Hori, Guy J Delespesse, Norihiko Watanabe, F.Xiao-Feng Qin, Zhengbin Yao, Wei Cao, Yong-Jun Liu
    • Journal Title

      J.Exp.Med. 202

      Pages: 1213-1223

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Nitric oxide inhibits IFN-α production of human plasmacytoid dendritic cells partly via a guanosine 3',5'-cyclic monophosphate-dependent pathway

    • Author(s)
      Rimpei Morita, Takashi Uchiyama, Toshiyuki Hori
    • Journal Title

      J. Immunol.

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi