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Exhaustive Allergenome Analysis of Japanese Cedar Pollen Allergen Molecules

Research Project

Project/Area Number 17607008
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field アレルギー
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

ONO Kazuhisa  Hiroshima University, Graduate School of Advanced Sciences of Matter, Professor, 大学院先端物質科学研究科, 教授 (10144883)

Co-Investigator(Kenkyū-buntansha) AKI Tsunehiro  Hiroshima University, Graduate School of Advanced Sciences of Matter, Associate Professor, 大学院先端物質科学研究科, 助教授 (80284165)
KAWAMOTO Seiji  Hiroshima University, Graduate School of Advanced Sciences of Matter, Research Associate, 大学院先端物質科学研究科, 助手 (90294537)
Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,700,000 (Direct Cost: ¥3,700,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥2,700,000 (Direct Cost: ¥2,700,000)
Keywordsallergen / cedar pollinosis / allergenome analysis / 感作アレルゲン / IgE頻度マップ / アレルゲン封入多角体 / プロテアーゼ / リパーゼ / ハイドロラーゼ / IgE反応頻度マップ / β-1,3-glucanase / aspartyl protease / 次世代診断システム / テーラーメイド医療
Research Abstract

Japanese cedar pollinosis is now a nation-wide allergic disease, although our knowledge about the causing factors, i.e. repertoire of Japanese cedar pollen (JCP) allergen molecules, is still limited. Objective of this study is exhaustive identification and characterization of JCP allergens via proteomics in conjunction with molecular immunological strategies, which we call "Allergenome Analysis".
(1) Mapping of JCP allergens
Two-dimensional (2D) IgE immunoblotting revealed that; 1) total of 131 allergen spots were found on the 2D map, and major allergens Cry j 1 and Cry j 2 spots distributed as multiple isoforms. 2) IgE-binding profile of each pollinosis patients was quite different from each other, suggesting that tailor-made molecular diagnosis is crucial for effective immunotherapy. 3) 31 allergen spots showed higher IgE binding frequency than that of Cry j 2 (40%).
(2) Identification and immunochemical characterization of new major JCP allergens
We next tried structural and immunochemical analyses of above new CJP allergens using TOF-MS analysis and cDNA cloning. We first identified class IV chitinase homologue (CJP4) as a novel major allergen with 100% IgE binding frequency, which is higher than that of Cry j 1 (71%). We also cloned two other allergens homologous to β-1,3-glucanase (CPA39) and aspartyl protease/nucleoid DNA binding protein family (CPA63), and functionally expressed those recombinant allergens using baculovirus-insect cell culture system. Intriguingly, we found that CJP4 and CPA39 showed IgE crossreactivity with other plant species such as latex or olive pollen, suggesting that JCP chitinase and/or β-1,3-glucanase may serve as pan-allergens involved in the pathogenesis of oral allergy syndrome.

Report

(3 results)
  • 2006 Annual Research Report   Final Research Report Summary
  • 2005 Annual Research Report
  • Research Products

    (26 results)

All 2007 2006 2005

All Journal Article (23 results) Book (1 results) Patent(Industrial Property Rights) (2 results)

  • [Journal Article] Dietary pulverized konjac glucomannan suppresses scratching behavior and skin inflammatory immune response in NC/Nga mice.2007

    • Author(s)
      Onishi N.
    • Journal Title

      Int. Arch. Allergy Immunol. (in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dietary pulverized konjac glucomannan suppresses scratching behavior and skin inflammatory immune response in NC/Nga mice.2007

    • Author(s)
      Onishi N., Kawamoto S., Suzuki H., Santo H., Aki T., Shigeta S., Hashimoto K., Hide M., Ono K.
    • Journal Title

      Int.Arch.Allergy Immunol. (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Dietary pulverized konjac glucomannan suppresses scratching behavior and skin inflammatory immune response in NC/Nga mice.2007

    • Author(s)
      N.Onishi et al.
    • Journal Title

      Int. Arch. Allergy Immunol. in press

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Decrease in the allergenicity of Japanese cedar pollen allergen by treatment with positive and negative cluster ions.2006

    • Author(s)
      Kawamoto S.
    • Journal Title

      Int. Arch. Allergy Immunol. 141

      Pages: 313-321

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Oral administration of pulverized Konjac glucomannan prevents the increase of plasma IgE and IgG levels induced by the injection of syngeneic keratinocyte extracts in BALB/c mice.2006

    • Author(s)
      Oomizu S.
    • Journal Title

      Clin. Exp. Allergy 36

      Pages: 102-110

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] プロテオーム解析によるスギ花粉・ダニアレルゲンの全容解明2006

    • Author(s)
      河本正次
    • Journal Title

      アレルギー・免疫 13

      Pages: 340-344

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] 免疫治療におけるスギ花粉抗原反復注射とダニ抗原反復注射によるTh1/Th2細胞応答の違いについて2006

    • Author(s)
      林 鷹治
    • Journal Title

      アレルギー科 21

      Pages: 308-320

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Decrease in the allergenicity of Japanese cedar pollen allergen by treatment with positive and negative cluster ions.2006

    • Author(s)
      Kawamoto S., Oshita M., Fukuoka N., Shigeta S., Aki T., Hayashi T., Nishikawa K., Ono K.
    • Journal Title

      Int.Arch.Allergy Immunol. 141

      Pages: 313-321

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Oral administration of pulverized Konjac glucomannan prevents the increase of plasma IgE and IgG levels induced by the injection of syngeneic keratinocyte extracts in BALB/c mice.2006

    • Author(s)
      Oomizu S., Onishi N., Suzuki H., Ueda K., Mochizuki M., Morimoto K., Kawamoto S., Ono K., Kameyoshi Y., Hide M.
    • Journal Title

      Clin.Exp.Allergy 36

      Pages: 102-110

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Decrease in the allergenicity of Japaniese cedar pollen allergen by treatment with positive and negative cluster ions.2006

    • Author(s)
      S.Kawamoto et al.
    • Journal Title

      Int. Arch. Allergy Immunol. 141

      Pages: 313-321

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Oral administration of pulverized Konjac glucomannan prevents the increase of plasma IgE and IgG levels induced by the injection of syngeneic keratinocyte extracts in BALB/c mice.2006

    • Author(s)
      S.Oomizu et al.
    • Journal Title

      Clin. Exp. Allergy 36

      Pages: 102-110

    • Related Report
      2006 Annual Research Report
  • [Journal Article] プロテオーム解析によるスギ花粉・ダニアレルゲンの全容解明2006

    • Author(s)
      河本正次 ら
    • Journal Title

      アレルギー・免疫 13

      Pages: 340-344

    • Related Report
      2006 Annual Research Report 2005 Annual Research Report
  • [Journal Article] 免疫治療におけるスギ花粉抗原反復注射とダニ抗原反復注射によるTh1/Th2細胞応答の違いについて2006

    • Author(s)
      林 鷹治 ら
    • Journal Title

      アレルギー科 21

      Pages: 308-320

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Molecular cloning of a class IV chitinase allergen from Japanese cedar (Cryptomeria japonica) pollen and competitive inhibition of its IgE-binding capacity by latex C-serum.2005

    • Author(s)
      Fujimura T.
    • Journal Title

      Clin. Exp. Allergy 35

      Pages: 234-243

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A new immunomodulatory function of low-viscous konjac-glucomannan with a small particle size : its oral intake suppresses spontaneously occurring dermatitis in NC/Nga mice.2005

    • Author(s)
      Onishi N.
    • Journal Title

      Int. Arch. Allergy Immunol. 136

      Pages: 258-265

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] スギ花粉アレルゲン解析の現状2005

    • Author(s)
      藤村孝志
    • Journal Title

      アレルギー科 19

      Pages: 557-562

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular cloning of a class IV chitinase allergen from Japanese cedar (Cryptomeria japonica) pollen and competitive inhibition of its IgE-binding capacity by latex C-serum.2005

    • Author(s)
      Fujimura T., Shigeta S., Suwa T., Kawamoto S., Aki T., Masubuchi M., Hayashi T., Hide M., Ono K.
    • Journal Title

      Clin.Exp.Allergy 35

      Pages: 234-243

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] A new immunomodulatory function of low-viscous konjac-glucomannan with a small particle size : its oral intake suppresses spontaneously occurring dermatitis in NC/Nga mice.2005

    • Author(s)
      Onishi N., Kawamoto S., Nishimura M., Nakano T., Aki T., Shigeta S., Shimizu H., Hashimoto K., Ono K.
    • Journal Title

      Int.Arch.Allergy Immunol. 136

      Pages: 258-265

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Journal Article] Molecular cloning of a class IV chitinase allergen from Japanese cedar (Cryptomeria japonica) pollen and competitive inhibition of its immunoglobulin E-binding capacity by latex C-serum2005

    • Author(s)
      T.Fujimura et al.
    • Journal Title

      Clin.Exp.Allergy 35

      Pages: 234-243

    • Related Report
      2005 Annual Research Report
  • [Journal Article] ダニアレルゲン-分子種構成、分子多型、ならびに特異的免疫治療-2005

    • Author(s)
      河本正次 ら
    • Journal Title

      アレルギー科 19

      Pages: 549-556

    • NAID

      40006852276

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Expression of a major house dust mite allergen gene from Dermatophagoides farinae in Lotus japonicus accession Miyakojima MG-202005

    • Author(s)
      T.Kato et al.
    • Journal Title

      J.Biosci.Bioeng. 99

      Pages: 165-168

    • Related Report
      2005 Annual Research Report
  • [Journal Article] スギ花粉アレルゲン解析の現状2005

    • Author(s)
      藤村孝志 ら
    • Journal Title

      アレルギー科 19

      Pages: 557-562

    • Related Report
      2005 Annual Research Report
  • [Journal Article] A newly identified class IV chitinase allergen from Japanese cedar pollen shows IgE crossreactivity with latex C-serum2005

    • Author(s)
      T.Fujimura et al.
    • Journal Title

      The XIXth World Allergy Organization Congress (Allergy & Clin.Immunol.Int.) Suppl.1

      Pages: 218-218

    • Related Report
      2005 Annual Research Report
  • [Book] アレルギー診断・治療の発展と展望-ホヤ喘息研究を通じて-(ホヤ抗原からダニ、スギ花抗原研究への展開)2005

    • Author(s)
      小埜和久(分担)
    • Publisher
      第55回日本アレルギー学会秋季大会
    • Related Report
      2005 Annual Research Report
  • [Patent(Industrial Property Rights)] スギ花粉由来の新規アレルゲン2005

    • Inventor(s)
      小埜和久 他
    • Industrial Property Rights Holder
      広島大学
    • Patent Publication Number
      2007-014311
    • Filing Date
      2005-07-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2006 Final Research Report Summary
  • [Patent(Industrial Property Rights)] スギ花粉由来の新規アレルゲン2005

    • Inventor(s)
      小埜 和久, 重田 征子, 秋 庸裕, 河本 正次, 島田 弥生, 力丸 智史, 大西 伸和, 大磯 勲
    • Industrial Property Rights Holder
      科学技術振興機構, 広島大学, 西川ゴム工業
    • Industrial Property Number
      2005-201947
    • Filing Date
      2005-07-11
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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