Transcription factors involve in expression of allergic-related genes
| Project/Area Number |
17607012
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
アレルギー
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| Research Institution | JUNTENDO UNIVERSITY |
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Principal Investigator |
NISHIYAMA Chiharu Juntendo University, School of Medicine, Assistant Professor, 医学部, 講師 (20327836)
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| Project Period (FY) |
2005 – 2006
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| Project Status |
Completed (Fiscal Year 2006)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | PU.1 / GATA-1 / Mast cells / Dendritic cells / IgE receptor / Allergy / Single Nucleotide Polymorphism / Hematopoietic stem cells / FOG-1 |
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| Research Abstract |
I am studying the cell type-specific gene expression mechanism in mast cells, which play important roles in IgE-mediated allergic reaction and are main target of anti-allergic drugs, because the suppression of mast cell-specific genes (especially FceRI) may provide novel tools for the prevention or treatment of allergic diseases. Previously, the study of transcriptional regulation mechanism of FcERI α-chain gene revealed that cooperation between transcription factors GATA-1 and PU.1 is one of mast cell-specific gene regulation mechanism (Nishiyama, J. Immunol. 2002). We also found that repression of GATA-1-dependent transactivation by cofactor FOG-1 causes mast cell-specific gene expression through the analysis of FcERI β-chain gene promoter in the recent study (Maeda, Blood 2006). Involvement of GATA-1 in FcERI expression was confirmed by the analysis of mast cells derived from GATA-1 knockdown mice (kindly provided by Prof. Masayuki Yamamoto, Tsukuba University). In addition, we reported that the expression level of PU.1 not only regulates monocyte-specific genes, but also determines cell fates including morphology and function (Ito, J. Immunol. 2005). Furthermore, we found that mast cells are a crucial source of functional IL-12 during the study of a transcription factor IRF-8, which forms heterodimer with PU.1 (Nakano, Blood, 2007). I was awarded the award for young scientists from Japan Society for Bioscience, Biotechnology, and Agrochemistry in 2005 and from Japanese Society for Allergology in 2006 through the above-mentioned studies.
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Report
(3 results)
Research Products
(43 results)
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[Journal Article] Involvement of mast cells in IL-12/23 p40 production is essential for survival from polvmicrobial infections.2007
Author(s)
Nakano, N., Nishiyama.C., Kanada, S., Niwa, Y., Shimokawa, N., Ushio, H., Nishiyama, M., Okumura, K., Ogawa, H.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Role of Sp1 in transcription of human ATP2A2 gene promoter in keratinocytes.2007
Author(s)
Takagi, A., Nishiyama.C., Maeda, K., Tokura, T., Kawada, H., Kanada, S., Niwa, Y., Nakano, N., Mayuzumi, N., Nishiyama, M., Ikeda, S., Okumura, K., Ogawa, H.
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Journal Title
T.Invest.Dermata (in press)
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] FOG-1 represses GATA-1- dependent FcεRI β-chain transcription : transcriptional mechanism of mast cell-specific gene expression in mice.2006
Author(s)
Maeda, K., Nishiyama, C., Tokura, T., Nakano, N., Kanada, S., Nishiyama, M., Okumura, K., Ogawa, H.
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Journal Title
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Prolonged MHC class II expression and CIITA transcription in human keratinocytes.2006
Author(s)
Takagi, A., Nishiyama.C., Kanada, S., Niwa, Y., Fukuyama, K., Ikeda, S., Okumura, K., Ogawa, H.
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Journal Title
Biochem.Biophys.Res.Commun. 347
Pages: 388-393
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Inhibitory effect of polyphenol-enriched apple extracts on mast cell degranulation in vitro targeting the binding between IgE and FcεRI.2005
Author(s)
Tokura, T., Nakano, N., Ito, T., Matsuda, H., Nagasako-Akazome, Y, Kanda, T., Ikeda, M., Okumura, K., Ogawa, H., Nishiyama.C.
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Journal Title
Biosci.Biotechnol.Biochem. 69
Pages: 1974-1977
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] GATA-1 is required for expression of FcεRI on mast cells : analysis of mast cells derived from GATA-1 knockdown mouse bone marrow.2005
Author(s)
Nishiyama.C., Ito, T., Nishiyama, M., Masaki, S., Maeda, K., Nakano, N., Ng, W, Fukuyama, K., Yamamoto, M., Okumura, K., Ogawa, H.
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Journal Title
Int.Immunol. 17
Pages: 847-856
NAID
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus.2005
Author(s)
Tubouchi, T., Mineki, R., Taka, H., Kaga, N., Murayama, K., Nishiyama, C., Yamane, H., Kuzuyama, T., Nishiyama, M.
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Journal Title
J.Biol.Chem. 280
Pages: 18511-18516
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Transcriptional regulation of ATP2C1 gene by Sp1 and YY1 and reduced function of its promoter in Hailey-Hailey disease keratinocytes.2005
Author(s)
Kawada, H., Nishiyama.C., Takagi, A., Tokura, T., Nakano, N., Maeda, K., Mayuzumi, N., Ikeda, S., Okumura, K., Ogawa, H.
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Journal Title
J.Invest.Dermatol. 124
Pages: 1206-1214
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Analysis of PU.1/ICSBP (IRF-8) complex formation with various PU.1 mutants : Molecular cloning of rat Icsbp (Irf-8) cDNA.2005
Author(s)
Nakano, N., Nishiyama, C., Masuoka, N., Nishiyama, M., Yamane, H., Okumura, K., Ogawa, H.
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Journal Title
Immunogenetics 56
Pages: 871-877
Description
「研究成果報告書概要(欧文)」より
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[Journal Article] Mast cells acquire monocyte-specific gene expression and monocyte- like morphology by overproduction of PU.1.2005
Author(s)
Ito, T., Nishiyama.C..Nishiyama, M., Matsuda, H., Maeda, K., Akizawa, Y., Tsuboi, R., Okumura, K., Ogawa, H.
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Journal Title
J.Immunol. 174
Pages: 376-383
Description
「研究成果報告書概要(欧文)」より
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