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粘土でロドプシンをつくる

Research Project

Project/Area Number 17657050
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionNagoya Institute of Technology

Principal Investigator

神取 秀樹  名古屋工業大学, 工学研究科, 教授 (70202033)

Project Period (FY) 2005 – 2006
Project Status Completed (Fiscal Year 2006)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2006: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 2005: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywords生物物理 / ナノバイオ / 生体分子 / 蛋白質 / 粘土 / 波長制御 / レチナール / ロドプシン / プロトンポンプ / 内部結合水 / 赤外分光法 / スイッチ / バクテリオロドプシン / 塩素イオンポンプ / 光受容蛋白質
Research Abstract

ロドプシンは動物や古細菌に存在する光受容蛋白質であり、レチナール発色団の光異性化反応を利用して光をエネルギーや情報へと変換する。レチナール分子を取り囲む蛋白質反応場がレチナールの電子状態を制御する結果、我々はさまざまな色を見ることができるのである。蛋白質場のはたらきを人工的に再現することはきわめて困難であったが、最近、粘土層間を用いて蛋白質と同じようなレチナール分子の波長制御が可能であることが報告された。そこで本研究では、粘土層問においてどのようなメカニズムでレチナール分子の波長制御が可能になっているのか明らかにすると同時に、異性化・プロトン移動といったロドプシンにおける機能の発現を粘土層間という新しい反応場において試みる。本年度は、特に色覚に関わるような波長制御といった観点から研究を行った結果、以下の成果を得た。
これまで粘土層間を界面活性剤で修飾し、疎水性と親水性の両方をもつ層間だけにロドプシンの発色団であるレチナールシッフ塩基がインターカレートされると考えられてきた。しかしながら、我々はベンゼン中でレチナールシッフ塩基と直接、加えることにより粘土層問への導入と可視領域の吸収の発現に成功した。そしてモンモリロナイトという粘土の産地に依存して色が変わることを発見した。この成果は蛋白質が行っている色覚の波長制御を、初めてそれ以外の系で実現したことに他ならず、現在、原著論文を投稿中である。
一方、粘土層問のコントロールとなる蛋白質反応場での光反応の研究は順調に伸展させることができ、2006年に15編の原著論文を報告した。また、平成18年度には9件の国際会議における招待講演を受けている。

Report

(2 results)
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (30 results)

All 2006 2005

All Journal Article (28 results) Book (2 results)

  • [Journal Article] Photoisomerization Processes in the 13-cis and All-trans Forms of Anabaena Sensory Rhodopsin at 77 K2006

    • Author(s)
      A.Kawanabe et al.
    • Journal Title

      Biochemistry 45・14

      Pages: 4362-4370

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Temperature-Dependent Interactions between Photoactivated pharaonis Phoborhodopsin and Its Transducer2006

    • Author(s)
      K.Kamada et al.
    • Journal Title

      Biochemistry 45・15

      Pages: 4859-4866

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Assignment of the Hydrogen-Out-Of-Plane and In-Plane Vibrations of the Retinal Chromonhore in the K Intermediate of pharaonis Phoborhodopsin2006

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Biochemistry 45・39

      Pages: 11836-11843

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Functional importance of the interhelical hydrogen bond between THR204 and TYR174 of sensory rhodopsin II and its alteration during the signaling process2006

    • Author(s)
      Y.Sudo et al.
    • Journal Title

      Journal of Biological Chemistry 281・45

      Pages: 34239-34245

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Conformational Coupling between the Cytoplasmic Carboxylic Acid and the Retinal in a Fungal Light-Driven Proton Pump2006

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Biochemistry 45・51

      Pages: 15349-15358

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Demura and Hideki Kandori, Deprotonation of Glu234 during the Photocycle of Natronomonas pharaonis Halorhodopsin2006

    • Author(s)
      M.Shibata et al.
    • Journal Title

      Chem. Phys. Lett. 432

      Pages: 545-547

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Photochemistry in Phototropin, a Blue Light Sensor Protein in Plants2006

    • Author(s)
      T.Iwata, H.Kandori
    • Journal Title

      Journal of the Chinese Chemical Society 53巻

      Pages: 67-73

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Photoreaction Cycle of the Light, Oxygen, and Voltage Domain in FKF1 Determined by Low-Temperature Absorption Spectroscopy2006

    • Author(s)
      K.Zikihara et al.
    • Journal Title

      Biochemistry 45・36

      Pages: 10828-10837

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Identification of the C=O Stretching Vibrations of FMN and Peptide Backbone by ^<13> C-Labeling of the LOV2 domain of Adiantun Phytochrome32006

    • Author(s)
      T.Iwata et al.
    • Journal Title

      Biochemistry 45・51

      Pages: 15384-15391

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Internal Water Molecules of the Proton-pumping Halorhodopsin in the Presence of Azide.2006

    • Author(s)
      N.Muneda et al.
    • Journal Title

      J. Am. Chem. Soc. 128

      Pages: 6294-6295

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Hydrogen-bonding Interaction of the Protonated Schiff Base with Halides in a Chloride-pumping Bacteriorhodopsin Mutant.2006

    • Author(s)
      M.Shibata et al.
    • Journal Title

      Biochemistry 45

      Pages: 10633-10640

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Structural Changes in Bacteriorhodopsin Following Retinal Photoisomerization from the 13-cis Form.2006

    • Author(s)
      N.Mizuide et al.
    • Journal Title

      Biochemistry 45

      Pages: 10674-10681

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Strongly Hydrogen-bonded Water Molecule is Observed only in the Alkaline Form of Proteorhodopsin.2006

    • Author(s)
      Y.Furutani et al.
    • Journal Title

      Chem. Phys. 324

      Pages: 705-708

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Water Structural Changes in the Activation Process of the LOV2 Domain of Adiantum Phytochrome3.2005

    • Author(s)
      Nozaki et al.
    • Journal Title

      J.Mol.Struct. 735/736

      Pages: 259-265

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Reactive Cysteine is Protonated in the Triplet Excited State of the LOV2 Domain in Adiantum Phytochrome3.2005

    • Author(s)
      Sato et al.
    • Journal Title

      J.Am.Chem.Soc. 127

      Pages: 1088-1089

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Structural Changes of the Complex between pharaonis Phoborhodopsin and Its Cognate Transducer upon Formation of the M Photointermediate.2005

    • Author(s)
      Furutani et al.
    • Journal Title

      Biochemistry 44

      Pages: 2909-2915

    • Related Report
      2005 Annual Research Report
  • [Journal Article] FTIR Studies of Internal Water Molecules in the Schiff Base Region of Bacteriorhodopsin.2005

    • Author(s)
      Shibata et al.
    • Journal Title

      Biochemistry 44

      Pages: 7406-7413

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Comparative Investigation of the LOV1 and LOV2 Domains in Adiantum Phytochrome3.2005

    • Author(s)
      Iwata et al.
    • Journal Title

      Biochemistry 44

      Pages: 7427-7434

    • Related Report
      2005 Annual Research Report
  • [Journal Article] FTIR Studies of the Photoactivation Processes in Squid Retinochrome.2005

    • Author(s)
      Furutani et al.
    • Journal Title

      Biochemistry 44

      Pages: 7988-7997

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Unique Temperature Dependence in the Adduct Formation between FMN and Cysteine S-H Group in the LOV2 Domain of Adiantum Phytochrome3.2005

    • Author(s)
      Nozaki et al.
    • Journal Title

      Chem.Phys.Lett. 410

      Pages: 59-63

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Hydrogen-Bonding Alterations of the Protonated Schiff Base and Water Molecule in the Chloride Pump of Natronobacterium pharaonis.2005

    • Author(s)
      Shibata et al.
    • Journal Title

      Biochemistry 44

      Pages: 12279-12286

    • Related Report
      2005 Annual Research Report
  • [Journal Article] FTIR Spectroscopy of the All-Trans Form of Anabaena Sensory Rhodopsin at 77 K : Hydrogen Bond of a Water between the Schiff Base and Asp75.2005

    • Author(s)
      Furutani et al.
    • Journal Title

      Biochemistry 44

      Pages: 12287-12296

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Strongly Hydrogen-Bonded Water Molecule Present near the Retinal Chromophore of Leptosphaeria Rhodopsin, the Bacteriorhodopsin-like Proton Pump from a Eukaryote.2005

    • Author(s)
      Sumii et al.
    • Journal Title

      Biochemistry 44

      Pages: 15159-15166

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Redox-induced Protein Structural Changes in Cytochrome bo Revealed by Fourier-transform Infrared Spectroscopy and ^<13>C-Tyr-labeling.2005

    • Author(s)
      Kandori et al.
    • Journal Title

      J.Biol.Chem. 280

      Pages: 32821-32826

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Steric Constraint in the Primary Photoproduct of an Archaeal Rhodopsin from Regiospecific Perturbation of C-D Stretching Vibration of the Retinyl Chromophore.2005

    • Author(s)
      Sudo et al.
    • Journal Title

      J.Am.Chem.Soc. 127

      Pages: 16036-16037

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Strongly Hydrogen-Bonded Water Molecules in the Schiff Base Region of Rhodopsins.2005

    • Author(s)
      Furutani et al.
    • Journal Title

      Photochem.Photobiol.Sci. 4

      Pages: 661-666

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Mechanism of Photoactivation in the LOV2 Domain of Adiantum Phytochrome3.2005

    • Author(s)
      Iwata et al.
    • Journal Title

      Recent Res.Devel.Biochem. 6

      Pages: 1-26

    • Related Report
      2005 Annual Research Report
  • [Journal Article] イオンポンプの機能にかかわる蛋白質内部の水分子2005

    • Author(s)
      神取秀樹
    • Journal Title

      現代化学 414

      Pages: 51-57

    • Related Report
      2005 Annual Research Report
  • [Book] cis-trans Isomerization in Biochemistry2006

    • Author(s)
      H.Kandori
    • Publisher
      Retinal binding proteins
    • Related Report
      2006 Annual Research Report
  • [Book] 細胞生物学事典(イオンポンプ)2005

    • Author(s)
      神取秀樹
    • Total Pages
      2
    • Publisher
      朝倉書店
    • Related Report
      2005 Annual Research Report

URL: 

Published: 2005-04-01   Modified: 2016-04-21  

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