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着床機構解明のための新規細胞株の樹立

Research Project

Project/Area Number 17658130
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field Basic veterinary science/Basic zootechnical science
Research InstitutionYamaguchi University

Principal Investigator

木曾 康郎 (木曽 康郎)  Yamaguchi University, 農学部, 教授 (10142374)

Co-Investigator(Kenkyū-buntansha) 本道 栄一  山口大学, 農学部, 准教授 (30271745)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2007: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2006: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2005: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywords子宮上皮 / 子宮腺上皮 / 再構築組織様塊 / 胎盤 / STAT3 / 細胞外マトリックス / LIF / エストロゲン / ウエスタンブロッティング
Research Abstract

本年度は最終年度にあたり、これまでに作成した新規細胞株の性状を総括し、さらに、マウスからの胎盤および子宮内膜の各構成細胞から作成したオルガノイド(再構築組織様塊)の有効性を検討した。オルガノイドと受胎産物あるいは子宮内膜との相互作用の解析から、子宮上皮細胞に対しては、白血病阻止因子(LIF)を添加した結果、シグナル伝達物質STAT3のリン酸化が起き、クローン細胞のエストロジェン添加培養はLIF分泌能を示したが、子宮腺上皮細胞には変化がみられなかった。細胞培養に細胞外マトリックスコーティングディッシュ(ECM-D)を使用した際に上皮細胞の極性に影響が現れ、ECM-Dの種類によって極性が変化した。さらに、エストロジェン添加によりその極性は著しい変化を示し、これらの変化が着床に関与していることが疑われた。着床現象は細胞外マトリックスと密接な関係にあるので、細胞外マトリックスと上皮細胞の極性の関係、それによって着床が影響を受けることがin vitroで再現、確認できた。次に、これらの細胞株を円筒状に培養し、子宮様再構築組織の作成を試み、上皮および腺上皮いずれも円筒状形態で培養に成功したが、導管部が構築されず、細胞外マトリックスと腺上皮細胞の極性の関係は上皮細胞のそれとは直接関係していないことが分かった。しかし、作成したオルガノイドの子宮内挿入実験では、明確な脱落膜反応を誘導し、炎症性サイトケインと非炎症性サイトカインの動態に差異が見られ、着床機構の研究ツールとして有用であると思われた。

Report

(3 results)
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (17 results)

All 2008 2007 2005

All Journal Article (13 results) (of which Peer Reviewed: 5 results) Presentation (4 results)

  • [Journal Article] Effects ofleukemia inhibitory factor on lectin-binding patterns in the uterine stromal vessels of mice.2008

    • Author(s)
      Wakitani S, 他7名
    • Journal Title

      Immunobiology (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Up-regulation of Indian hedgehog gene in the uterine epithelium by leukemia inhibitory factor during mouse implantation2008

    • Author(s)
      Wakitani S, 他4名
    • Journal Title

      J Reprod Dev (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of a novel bat adenovirus from a fruit bat(Pteropus dasymallus yaeyamae)2008

    • Author(s)
      Maeda K, 他8名
    • Journal Title

      Emerging lnfect Dis (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution patterns of uterine glands and embryo spacing in the mouse2007

    • Author(s)
      Hondo E, 他6名
    • Journal Title

      Anat Histol Embryol 36

      Pages: 157-159

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of bisphenol A(BPA)on placentation and survival of the neonates in mice2007

    • Author(s)
      Tachibana T, 他6名
    • Journal Title

      J Reprod Dev 53

      Pages: 509-514

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distribution patterns of uterine glands and embryo spacing in the mouse.2007

    • Author(s)
      Hondo E, 他6名
    • Journal Title

      Anat Histol Embryol (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of bisphenol A (BPA) on placentation and survival of the neonates in mice.2007

    • Author(s)
      Tachibana T, 他7名
    • Journal Title

      J Reprod Dev (In press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Perforin improves the morphogenesis of mouse placenta disturbed by IL-2 treatment.2005

    • Author(s)
      Kusakabe K, LiZ-L, Kiso Y, Otsuki Y.
    • Journal Title

      Immunobiology 209

      Pages: 719-728

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Involvement of the Fas ligand and Fas system in apoptosis induction of mouse uterine natural killer cells.2005

    • Author(s)
      Kusakabe K, Otsuki Y, Kiso Y.
    • Journal Title

      J Reprod Dev 51

      Pages: 333-340

    • NAID

      10027324869

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Ultrastructural study of uterine natural killer cells found in pregnant, interleukin-2 receptor b-chain overexpressed transgenic mice.2005

    • Author(s)
      Kokubu K, Hondo E, Nambe Y, Kusakabe K, Sagara E, Kiso Y.
    • Journal Title

      J Reprod Dev 51

      Pages: 695-698

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Differentiation and elimination of uterine natural killer cells in delayed implantation and parturition Mice.2005

    • Author(s)
      Kokubu K, Hondo E, Sakaguchi N, Kiso Y.
    • Journal Title

      J Reprod Dev 51

      Pages: 773-776

    • NAID

      10027326583

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Localization of interleulin-6 receptor mRNA in pregnant and non-pregnant mouse uterus.2005

    • Author(s)
      Hondo E, Kokubu K, Kato K, Kiso Y.
    • Journal Title

      J Reprod Dev 51

      Pages: 777-781

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Histological study of granulated metrial gland (GMG) cells in Beige (DA-bg/bg) rats.2005

    • Author(s)
      Hondo E, Kokubu K, Nagahisa A, Nakamuta N, Ozaki K, Sagara E, Kiso Y.
    • Journal Title

      J Reprod Dev 51

      Pages: 799-803

    • NAID

      10027326688

    • Related Report
      2005 Annual Research Report
  • [Presentation] Gene expression analysis of the uterine epithelium between the mesometrial and anti-mesometrial side during implantation in mice.2007

    • Author(s)
      Sugiyama T, 他2名
    • Organizer
      The 2nd Conference of Asian Association of Veterinary Anatomists
    • Place of Presentation
      Bangkok,Thai
    • Year and Date
      2007-09-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] Necessity of uterine natural killer(uNK)cells in angioarchitecture of the implantation site of mice.2007

    • Author(s)
      Pichitraslip T, 他3名
    • Organizer
      The 2nd Conference of Asian Association of Veterinary Anatomists
    • Place of Presentation
      Bangkok,Thai
    • Year and Date
      2007-09-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] Induction of Indian hedgehog mRNA in the uterine luminal epithelium by leukemia inhibitory factor.2007

    • Author(s)
      Wakitani S, 他3名
    • Organizer
      The 2nd Conference of Asian Association of Veterinary Anatomists
    • Place of Presentation
      Bangkok,Thai
    • Year and Date
      2007-09-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] Leukemia inhibitory factor(LIF)affects lectin-bindings to the uterine stromal blood vessel.2007

    • Author(s)
      Terakawa J, 他4名
    • Organizer
      The 2nd Conference of Asian Association of Veterinary Anatomists
    • Place of Presentation
      Bangkok,Thai
    • Year and Date
      2007-09-10
    • Related Report
      2007 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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