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口腔微生物叢の「揺りかご」:舌苔バイオフィルムの生物学

Research Project

Project/Area Number 17659659
Research Category

Grant-in-Aid for Exploratory Research

Allocation TypeSingle-year Grants
Research Field Social dentistry
Research InstitutionTohoku University

Principal Investigator

高橋 信博  Tohoku University, 大学院・歯学研究科, 教授 (60183852)

Co-Investigator(Kenkyū-buntansha) 小関 健由  東北大学, 大学院・歯学研究科, 教授 (80291128)
佐藤 拓一  東北大学, 大学院・歯学研究科, 講師 (10303132)
鷲尾 純平  東北大学, 大学院・歯学研究科, 助教 (20400260)
今井 奨  国立保健医療科学院, 口腔保健部, 室長 (80072958)
Project Period (FY) 2005 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2007: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2005: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywords歯学 / 舌苔 / 微生物 / バイオフィルム / 口臭 / 硫化水素 / Veillonella / Actinomyces / Prevotlla / システイン / グルタチオン / Prevotella
Research Abstract

本年度は、昨年度に引き続き、口臭原因菌の一つと捉えられる口腔Veillonella属の硫化水素産生活性について検討し、(1)環境因子、とくに口腔内に多く存在する乳酸等の有機酸が硫化水素産生能に与える影響、及び、(2)硫化水素産生に関わる代謝酵素の検索を行った。培養したVeillonella atypica、 V. dispar、 V. parvulaの各標準株(ATCC株)の培養菌にシステインを加えて3時間インキュベートし、産生した硫化水素をメチレンブルー法で定量した。さらにこの反応系に乳酸等の各種有機酸を加え、硫化水素産生活性への影響を検討した。また、培養菌を超音波処理して無細胞抽出液を調整し、システインからの硫化水素産生に関わる酵素活性を検索した。Veillonellaのシステインからの硫化水素産生活性は、乳酸が存在すると著しく増加した。Veillonellaの無細胞抽出液は、システインから硫化水素を産生する酵素活性を示したが、無細胞抽出液に乳酸を添加しても活性の増加は認められなかった。以上のことから、Veillonellaは乳酸の存在下、システインから効率よく硫化水素を産生することが明らかになった。乳酸による促進作用は、酵素の活性化作用ではなく、システインの取込み促進であるものと予測された。口臭源の一つである硫化水素は口腔細菌によって産生されるが、その産生活性はpH(昨年度成果)や有機酸(今年度成果)という口腔環境によって影響を受けるものと考えられ、口腔環境のコントロールによって口臭を抑制し得るものと考えられる。

Report

(3 results)
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • 2005 Annual Research Report
  • Research Products

    (29 results)

All 2008 2007 2006 2005

All Journal Article (14 results) (of which Peer Reviewed: 4 results) Presentation (3 results) Book (12 results)

  • [Journal Article] Chairside-evaluation of pH-lowering activity and lactic acid production of dental plaque: Correlation with caries-experience and incidence incidence in preschool children2008

    • Author(s)
      Shimizu K
    • Journal Title

      Quintessence Int 39

      Pages: 151-158

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mutual induction of noncollagenous bone proteins at the interface between epithelial cells and fibroblasts from human periodontal ligament2008

    • Author(s)
      Shimonishi M
    • Journal Title

      J Periodontal Res 43

      Pages: 64-75

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Profiling of bacterial flora in crevices around titanium orthodontic anchor plates2007

    • Author(s)
      Sato R
    • Journal Title

      Clin Oral Implants Res 18

      Pages: 21-26

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro differentiation of epithelial cells cultured from human periodontal ligament2007

    • Author(s)
      Shimonishi M
    • Journal Title

      J Periodontal Res 42

      Pages: 456-465

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Profiling of bacterial flora in crevices around titanium orthodontic anchor plates2007

    • Author(s)
      Sato R
    • Journal Title

      Clin Oral Implant Res 18(1)

      Pages: 21-26

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Resistance to acidic and alkaline environments in the endodontic pathogen Enterococcus faecalis2006

    • Author(s)
      Nakajo K
    • Journal Title

      Oral Microbiol Immunol 21(5)

      Pages: 283-288

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains, and its biochemical mechanism2006

    • Author(s)
      Miyasawa-Hori H
    • Journal Title

      Oral Microbiol Immunol 21(4)

      Pages: 201-205

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Comparison of age-dependent expression of aggrecan and ADAMTSs in mandibular condylar cartilage, tibial growth plate, and articular cartilage in rats2006

    • Author(s)
      Mitani H
    • Journal Title

      Histochem Cell Biol 126(3)

      Pages: 371-380

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 培養ヒト歯根膜細胞由来上皮細胞と線維芽細胞の境界面におけるオステオポンチンおよびオステオカルシンの発現2006

    • Author(s)
      佐藤充太
    • Journal Title

      日歯保存誌 49(1)

      Pages: 92-98

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Difference in the xylitol sensitivity of acid production among Streptococcus mutans strains, and its biochemical mechanism.2006

    • Author(s)
      Miyasawa-Hori H
    • Journal Title

      Oral Microbiol Immunol 21(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Resistance to acidic and alkaline environments in the endodontic pathogen Enterococcus faecalis.2006

    • Author(s)
      Nakajo K
    • Journal Title

      Oral Microbiol Immunol 21(in press)

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Hydrogen sulfide-producing bacteria in tongue coating and their relationship with oral malodour.2005

    • Author(s)
      Washio J
    • Journal Title

      J Med Microbiol 54(9)

      Pages: 889-895

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Synergistic inhibition by combination of fluoride and xylitol on the glycolysis by mutans streptococci and its biochemical mechanism.2005

    • Author(s)
      Maehara H
    • Journal Title

      Caries Res 39(6)

      Pages: 521-528

    • Related Report
      2005 Annual Research Report
  • [Journal Article] Quantification and detection of bacteria from postoperative maxillary cyst by polymerase chain reaction.2005

    • Author(s)
      Yamaura M
    • Journal Title

      Oral Microbiol Immunol 20(6)

      Pages: 333-338

    • Related Report
      2005 Annual Research Report
  • [Presentation] 口腔環境中の乳酸はVeillonellaの硫化水素産生を促進する2007

    • Author(s)
      鷲尾純平
    • Organizer
      第56回日本口腔衛生学会
    • Place of Presentation
      千葉
    • Year and Date
      2007-10-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] 口腔 Veillonella はcystathionine β-synthase様活性を持ちシステインから硫化水素を産生する2007

    • Author(s)
      鷲尾純平
    • Organizer
      第49回歯科基礎医学会学術大会
    • Place of Presentation
      札幌
    • Year and Date
      2007-08-30
    • Related Report
      2007 Annual Research Report
  • [Presentation] Effects of pH and lactate on the production of hydrogen sulfide by oral Veillonella2007

    • Author(s)
      Washio J
    • Organizer
      7th International conference of the international Society for Breath Odor Research
    • Place of Presentation
      米国シカゴ
    • Year and Date
      2007-08-23
    • Related Report
      2007 Annual Research Report
  • [Book] う蝕学-チェアサイドの予防と回復のプログラム-2008

    • Author(s)
      高橋信博
    • Publisher
      末長書店
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Washio J
    • Publisher
      Springer,Tokyo
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Abiko Y
    • Publisher
      Springer, Tokyo
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Matsuyama J
    • Publisher
      Springer, Tokyo
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Miyasawa-Hori H
    • Publisher
      Springer, Tokyo
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Aizawa S
    • Publisher
      Springer, Tokyo
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Nakajo K
    • Publisher
      Springer, Tokyo
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Ito Y
    • Publisher
      Springer, Tokyo
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Sato T
    • Publisher
      Springer, Tokyo
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Masaki M
    • Publisher
      Springer, Tokyo
    • Related Report
      2007 Annual Research Report
  • [Book] Interface Oral Health Science 20072007

    • Author(s)
      Miyoshi Y
    • Publisher
      Springer, Tokyo
    • Related Report
      2007 Annual Research Report
  • [Book] 口腔生化学 第4版2005

    • Author(s)
      早川太郎, 須田立雄, 木崎治俊, 畑隆一郎, 高橋信博, 宇田川信之
    • Total Pages
      357
    • Publisher
      医歯薬出版株式会社
    • Related Report
      2005 Annual Research Report

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Published: 2005-04-01   Modified: 2016-04-21  

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