| Project/Area Number |
17F17117
|
|---|
| Research Category |
Grant-in-Aid for JSPS Fellows
|
| Allocation Type | Single-year Grants |
|---|
| Section | 外国 |
|---|
| Research Field |
Parasitology (including sanitary zoology)
|
|---|
| Research Institution | Osaka University |
|---|
Principal Investigator |
Coban Cevayir 大阪大学, 免疫学フロンティア研究センター, 教授 (00397712)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
LELLIOTT PATRICK 大阪大学, 免疫学フロンティア研究センター, 外国人特別研究員
|
|---|
| Project Period (FY) |
2017-04-26 – 2019-03-31
|
| Project Status |
Completed (Fiscal Year 2018)
|
| Budget Amount *help |
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2018: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2017: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
|---|
| Keywords | Imaging Flow Cytometry / NETs / Netosis / Extracellular traps / Malaria |
|---|
| Outline of Annual Research Achievements |
This project investigated the role of neutrophil extracellular traps (NETs) during malaria infection. The major achievement of this project was to develop an accurate, high throughput method for the characterization of NETs. We achieved this through the development of a novel 3D cell culture method which we used to maintain a single cell solution of neutrophils and NETs. Cells were analyzed by imaging flow cytometry and six distinct cell types could be identified, including NETs, NET precursors, and non-NET dead cells. An automated analysis algorithm was development to quantify these cell types rapidly and in an unbiased manner. We applied this method to provide the first analysis of NETs in the circulation in mice infected with the murine malaria parasite Plasmodium yoelii.
|
| Research Progress Status |
平成30年度が最終年度であるため、記入しない。
|
| Strategy for Future Research Activity |
平成30年度が最終年度であるため、記入しない。
|
