Development of a new high-throughput TCR-antigen pair identification method toward cancer immune therapy
| Project/Area Number |
17F17389
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| Research Category |
Grant-in-Aid for JSPS Fellows
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| Allocation Type | Single-year Grants |
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| Section | 外国 |
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| Research Field |
Genome biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
城口 克之 国立研究開発法人理化学研究所, 生命機能科学研究センター, チームリーダー (00454059)
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| Co-Investigator(Kenkyū-buntansha) |
JIN JIANSHI 国立研究開発法人理化学研究所, 生命機能科学研究センター, 外国人特別研究員
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| Project Period (FY) |
2017-10-13 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2019: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 2018: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 2017: ¥1,200,000 (Direct Cost: ¥1,200,000)
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| Keywords | イメージング / ピッキング / 細胞相互作用 / Ｔ細胞 / TCR / T cell / sequencing / cell-cell interaction / microscopy |
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| Outline of Annual Research Achievements |
We have been building up a microscopic platform for scanning the real-time cell behaviors, such as cell movement and T cell-target cell interactions. For high-throughput scanning of these behaviors, we have checked that scanning rate of the current system, and found that the speed of stage movement may be enough for the observation of T cell activities. However, in order to link the microscope system to following steps, we found that real-time calculation based on obtained cell images was required. Then, we investigated specifications of a software based on our actual measurements for T cell-target cell interactions. This helps one to combine imaging and following genomic analyses using our cell picking system.
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| Research Progress Status |
令和元年度が最終年度であるため、記入しない。
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| Strategy for Future Research Activity |
令和元年度が最終年度であるため、記入しない。
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Report
(3 results)
Research Products
(4 results)
