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Mite biodiversity and their taxonomy using morphological and molecular-based identifications

Research Project

Project/Area Number 17F17397
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Plant protection science
Research InstitutionIbaraki University

Principal Investigator

北嶋 康樹 (2018-2019)  茨城大学, 農学部, 准教授 (00431651)

後藤 哲雄 (2017)  茨城大学, 農学部, 教授 (60178449)

Co-Investigator(Kenkyū-buntansha) NEGM MOHAMED  茨城大学, 農学部, 外国人特別研究員
Project Period (FY) 2017-11-10 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥2,300,000 (Direct Cost: ¥2,300,000)
Fiscal Year 2019: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 2018: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2017: ¥800,000 (Direct Cost: ¥800,000)
Keywordsダニ / 分類 / 系統関係 / ダニ・線虫管理
Outline of Annual Research Achievements

本研究では、様々な生息環境に生息するダニ類を網羅的に採集し、形態による分類と分子情報による識別を行うことによって、生息環境ごとのダニ相の多様性を解析するとともに、特に農業上重要と思われる種については、分子情報による簡易なダニの識別法の開発も視野に入れて研究を進めてきた。2019年度は、千葉県の照葉樹林、岩手県の落葉樹林ならびに果樹園、栃木県の落葉広葉樹林、茨城県および埼玉県の二次林で調査を実施した。採集法は、植物上のダニ類を直接はたき落とす方法と、土壌を採取してツルグレン法による方法を併用した。採集したダニ類は、プレパラート標本を作成するとともに、ミトコンドリアDNAのCOⅠ領域のシーケンスを実施した。その結果、ケダニ亜目から11科13種、トゲダニ亜目から7科9種のダニが発見され、これらのうち、千葉県から採集された2種(ケダニ亜目)、ならびに茨城県から採集された1種(トゲダニ亜目)は新種であることが明らかとなり、これらを2報の国際誌で発表した(Negm & Gotoh, 2019; Negm et al., 2020)。また、千葉県南房総市の照葉樹林からは、日本に土着していないとされるチリカブリダニも採集され、農耕地以外にも本種が定着している可能性が示された。さらに、ミズナラクダハダニ(Amphytetranychus quercivorus)とその近縁種である A. savenkoaeの関係を形態とDNAの観点から考察し、A. savenkoaeの再記載に関わる国際共同研究に加わった(Arabuli et al., 2019)。

Research Progress Status

令和元年度が最終年度であるため、記入しない。

Strategy for Future Research Activity

令和元年度が最終年度であるため、記入しない。

Report

(3 results)
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (15 results)

All 2020 2019 2018 Other

All Int'l Joint Research (5 results) Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (3 results) Remarks (1 results)

  • [Int'l Joint Research] Ilia State University(ジョージア)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Schmalhausen Institute of Zoology(ウクライナ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] North-West University(南アフリカ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Wroclaw Univ. of Environ. and Life Sci.(ポーランド)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Montenegro(モンテネグロ)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] A new species of Cenopalpus Pritchard & Baker (Acari: Tenuipalpidae) from Japan, with ontogeny of chaetotaxy and a key to the world species2020

    • Author(s)
      Negm Mohamed W.、Ueckermann Edward A.、Gotoh Tetsuo
    • Journal Title

      PeerJ

      Volume: 8 Pages: e9081-e9081

    • DOI

      10.7717/peerj.9081

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Morphological identification of Amphitetranychus species (Acari: Tetranychidae) with crossbreeding, esterase zymograms and DNA barcode data2019

    • Author(s)
      Arabuli Tea、Negm Mohamed Waleed、Matsuda Tomoko、Kitashima Yasuki、Abramishvili Tea、Akimov Igor Andrijovych、Zhovnerchuk Olga Valentynivna、Popov Sergei Yakovlevich、Gotoh Tetsuo
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 9 Pages: 1-22

    • DOI

      10.1371/journal.pone.0221951

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] New record and redescription of Mullederia sichuanensis Wang from evergreen forests in Japan, with remarks on morphological variations among world species of Mullederia Wood (Acari: Stigmaeidae)2019

    • Author(s)
      Negm Mohamed W.、Gotoh Tetsuo
    • Journal Title

      Systematic and Applied Acarology

      Volume: 24 Issue: 7 Pages: 1150-1161

    • DOI

      10.11158/saa.24.7.4

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Redescription of Agistemus lobatus Ehara, 1964 and A. terminalis (Quayle, 1912) (Acari: Trombidiformes: Stigmaeidae) with DNA barcoding2019

    • Author(s)
      Negm, M. W. and T. Gotoh
    • Journal Title

      Systematic and Applied Acarology

      Volume: 24 Issue: 1 Pages: 33-44

    • DOI

      10.11158/saa.24.1.3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A new larval Lassenia Newell, 1957 (Trombidiformes: Tanaupodidae) from Japan2019

    • Author(s)
      Haitlinger, R., M. W. Negm, T. Gotoh and S. Miloge
    • Journal Title

      Systematic and Applied Acarology

      Volume: 24 Issue: 2 Pages: 271-277

    • DOI

      10.11158/saa.24.2.9

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mites of the family Parasitidae Oudemans, 1901 (Acari: Mesostigmata) from Japan: a new species of Vulgarogamasus Tichomirov, 1969, and a key to Japanese species2018

    • Author(s)
      Negm, M. W. and T. Gotoh
    • Journal Title

      Zootaxa

      Volume: 4429 Issue: 2 Pages: 379-389

    • DOI

      10.11646/zootaxa.4429.2.12

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] New findings of manure-inhabiting mites of the family Parasitidae (Mesostigmata: Gamasina) from Japan, a voucher collection-based study2019

    • Author(s)
      Negm, M.W, T. Gotoh, H. Nemoto and Y. Ito
    • Organizer
      第28回日本ダニ学会大会富山大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Redescription of Agistemus lobatus Ehara, 1964 and A. terminalis (Quayle, 1912) (Acari: Stigmaeidae) with DNA barcoding2019

    • Author(s)
      Negm, Mohamed W. and Testuo Gotoh
    • Organizer
      第63回日本応用動物昆虫学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Mullederia Wood, 1964 (Acari: Stigmaeidae) mites newly recorded from Japan, with redescription of M. sichuanensis Wang, 1986 and DNA barcode data2018

    • Author(s)
      Negm, Mohamed W. and Tetsuo Gotoh
    • Organizer
      第27回日本ダニ学会大会
    • Related Report
      2018 Annual Research Report
  • [Remarks] http://www.aun.edu.eg/membercv_print.php?M_ID=1368

    • Related Report
      2019 Annual Research Report

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Published: 2017-11-13   Modified: 2024-03-26  

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