熱ショックにより植物に誘導される病害抵抗性の新規メカニズムの解明

Research Project

Project/Area Number	17F17398
Research Category	Grant-in-Aid for JSPS Fellows
Allocation Type	Single-year Grants
Section	外国
Research Field	Plant protection science
Research Institution	Ibaraki University
Principal Investigator	佐藤達雄茨城大学, 農学部, 教授 (20451669)
Co-Investigator(Kenkyū-buntansha)	WIDIASTUTI ANI 茨城大学, 農学部, 外国人特別研究員
Project Period (FY)	2017-11-10 – 2019-03-31
Project Status	Completed (Fiscal Year 2018)
Budget Amount *help	¥1,100,000 (Direct Cost: ¥1,100,000) Fiscal Year 2018: ¥700,000 (Direct Cost: ¥700,000) Fiscal Year 2017: ¥400,000 (Direct Cost: ¥400,000)
Keywords	Heat shock / Induced resistance / Cucumber / Powdery mildew / Heat shock element / Inducing resistance / Heat shock protein
Outline of Annual Research Achievements	Aims of the proposed research are to observe activation of some genes related to Induced Resistance Systemic (ISR) or Jasmonate pathway, additionally to systemic acquired resistance (SAR) in Heat shock-induced resistance (HSIR), and to study whether heat shock proteins play roles in HSIR and the time if those HSPs take involve in HSIR mechanism. Also, to study whether the expressed genes possess particular heat shock element (HSE) that possibly involve in heat shock transcriptional factors (HSF). As the result of this study, we investigated HSIR against cucumber powdery mildew and confirmed initial mechanism of HSIR prior to SAR. Gene expression of Hsp70 were upregulated at 12 hour, followed by peroxidase and lipoxygenase at 24 hour after heat shock treatment. Putative HSE motifs were found in upstream area of those target genes. HSFs such as HsfA2, HsfB1 and HsfB4 gene expressions increased from 6 h after heat shock, which confirm that upon heat stress, some Hsfs were activated to bind HSE and upregulate transcription of Hsp70 and defense-related genes. From the results of previous study, HSIR has been considered to include SAR. We found a time lag between the expression of marker genes concerning these two pathways that suggested they were independent reactions that influence each other.
Research Progress Status	平成30年度が最終年度であるため、記入しない。
Strategy for Future Research Activity	平成30年度が最終年度であるため、記入しない。