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泌尿器転移癌患者の循環血液中癌細胞の遺伝子解析

Research Project

Project/Area Number 17F17735
Research Category

Grant-in-Aid for JSPS Fellows

Allocation TypeSingle-year Grants
Section外国
Research Field Urology
Research InstitutionJuntendo University

Principal Investigator

堀江 重郎  順天堂大学, 医学(系)研究科(研究院), 教授 (40190243)

Co-Investigator(Kenkyū-buntansha) HOU QI  順天堂大学, 医学(系)研究科(研究院), 外国人特別研究員
Project Period (FY) 2017-11-10 – 2020-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥2,200,000 (Direct Cost: ¥2,200,000)
Fiscal Year 2018: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 2017: ¥200,000 (Direct Cost: ¥200,000)
Keywords血中循環腫瘍細胞 / 去勢抵抗性前立腺癌 / Precision medicine / PSMA / バイオマーカー
Outline of Annual Research Achievements

CRPCを含む進行前立腺がん86例へ、治療経過で継時的にCTC解析を行なった。CTC回収方法は、抗体蛍光染色によるCytoQuest(Abnova社)、抗体磁気ビーズによるAdnaTest(Qiagen社)を用いた。AR-V7(androgen receptor splicing variant 7)の有無やAR変異の有無、PSMA(prostate-specific membrane antigen)などの前立腺がん関連発現分子の発現を主に解析した。86例から延べ267回のCTC検出を試み、検出率は66.1%(154/267)であった。CTC検出可能であった患者は、採取時の腫瘍マーカーPSA値が有意に高かった(p < 0.001)。AR-V7発現率は19%(14/72人)で、AR-V7陰性CRPCに対する新規内分泌治療薬の奏効率は87%(13/15)であった。また、CTCにおけるPSMAの発現は62%であり、PSMA, AR, AR-V7 およびEGFR発現は治療の2nd-line以降の患者、つまりより進行している患者で有意に陽性率が高いという結果になっ(p<0.05)。 臨床的に治療を変更した56人の症例のうち、39例でCTC検出でき、またそのうち20例でPSMAが陽性であった。PSMA発現は、治療によるPSA減少と有意に逆相関していた。
今後のPSMAの機序の解明として、in vitroの系でPSMAの機能解析を行っている。現在、PSMA発現が陰性の前立腺がん細胞株にPSMA発現ベクターをtransfectionして安定発現株を作成している。

Research Progress Status

翌年度、交付申請を辞退するため、記入しない。

Strategy for Future Research Activity

翌年度、交付申請を辞退するため、記入しない。

Report

(2 results)
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (22 results)

All 2019 2018 2017

All Journal Article (10 results) (of which Int'l Joint Research: 9 results,  Peer Reviewed: 9 results,  Open Access: 9 results) Presentation (12 results) (of which Int'l Joint Research: 9 results,  Invited: 1 results)

  • [Journal Article] Surveillance after prostate focal therapy2019

    • Author(s)
      Tay KJ, Amin MB, Ghai S, Jimenez RE, Kench JG, Klotz L, Montironi R, Muto S, Rastinehad AR, Turkbey B, Villers A, Polascik TJ
    • Journal Title

      World J Urol

      Volume: Mar;37(3): Issue: 3 Pages: 397-407

    • DOI

      10.1007/s00345-018-2363-y

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Surge in the Number of Circulating Tumor Cells Following Treatment with Sunitinib for Metastatic Renal Cell Carcinoma2018

    • Author(s)
      Nagaya Naoya、Kanayama Mayuko、Nagata Masayoshi、Horie Shigeo
    • Journal Title

      Internal Medicine

      Volume: 57 Issue: 18 Pages: 2695-2700

    • DOI

      10.2169/internalmedicine.0663-17

    • NAID

      130007484141

    • ISSN
      0918-2918, 1349-7235
    • Year and Date
      2018-09-15
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Association of TUSC1 and DPF3 gene polymorphisms with male infertility2018

    • Author(s)
      Sato Youichi、Hasegawa Chise、Tajima Atsushi、Nozawa Shiari、Yoshiike Miki、Koh Eitetsue、Kanaya Jiro、Namiki Mikio、Matsumiya Kiyomi、Tsujimura Akira、Komatsu Kiyoshi、Itoh Naoki、Eguchi Jiro、Yamauchi Aiko、Iwamoto Teruaki
    • Journal Title

      Journal of Assisted Reproduction and Genetics

      Volume: 35 Issue: 2 Pages: 257-263

    • DOI

      10.1007/s10815-017-1052-x

    • NAID

      120006768430

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Efficacy and safety of 3-month dosing regimen of degarelix in Japanese subjects with prostate cancer: A phase III study2018

    • Author(s)
      Ozono Seiichiro、Tsukamoto Taiji、Naito Seiji、Horie Shigeo、Ohashi Yasuo、Uemura Hiroji、Yokomizo Yumiko、Fukasawa Satoshi、Kusuoka Hidehito、Akazawa Rio、Saito Masako、Akaza Hideyuki
    • Journal Title

      Cancer Science

      Volume: 109 Issue: 6 Pages: 1920-1929

    • DOI

      10.1111/cas.13600

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Modulation of AKR1C2 by Curcumin Decreases Testosterone Production in Prostate Cancer2018

    • Author(s)
      Ide H, Lu Y, Noguchi T, Muto S, Okada H, Kawato S and Horie S
    • Journal Title

      Cancer Science

      Volume: 109 Issue: 4 Pages: 1230-1238

    • DOI

      10.1111/cas.13517

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Generation of transgenic mouse line with prostate-specific expression of codon-improved Cre recombinase2018

    • Author(s)
      Kanayama M, Nakao K, Horie S, Aiba A
    • Journal Title

      Prostate Int.

      Volume: Sep;6(3). Pages: 99-103

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Clinical evaluation of two consecutive UroVysion fluorescence in situ hybridization tests to detect intravesical recurrence of bladder cancer: a prospective blinded comparative study in Japan2018

    • Author(s)
      Kojima Takahiro、Nishiyama Hiroyuki、Ozono Seiichiro、Hinotsu Shiro、Keino Naoto、Yamaguchi Akito、Sakai Hideki、Enomoto Yutaka、Horie Shigeo、Fujimoto Kiyohide、Matsuyama Hideyasu、Okamura Takehiko、Kanimoto Yusuke、Oya Mototsugu、Nonomura Norio、Naito Seiji、Akaza Hideyuki
    • Journal Title

      International Journal of Clinical Oncology

      Volume: 23 Issue: 6 Pages: 1140-1147

    • DOI

      10.1007/s10147-018-1311-6

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] RankProd Combined with Genetic Algorithm Optimized Artificial Neural Network Establishes a Diagnostic and Prognostic Prediction Model that Revealed C1QTNF3 as a Biomarker for Prostate Cancer.2018

    • Author(s)
      Hou Q, Bing ZT, Hu C, Li MY, Yang KH, Mo Z, Xie XW, Liao JL, Lu Y, Horie S, Lou MW.
    • Journal Title

      EBioMedicine

      Volume: Jun;32 Pages: 234-244

    • DOI

      10.1016/j.ebiom.2018.05.010

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 座談会 前立腺癌研究の最前線(第3回)診断における最新の知見と今後の展望2018

    • Author(s)
      堀江 重郎 , 和久本 芳彰 , 永田 政義 , 井手 久満 , 松下 一仁
    • Journal Title

      Prostate journal

      Volume: 10;5(2) Pages: 205-209

    • NAID

      40021710097

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Guidelines for treatment of renal injury during cancer chemotherapy 20162017

    • Author(s)
      Horie Shigeo、Oya Mototsugu、Nangaku Masaomi、Yasuda Yoshinari、Komatsu Yasuhiro、Yanagita Motoko、Kitagawa Yuko、Kuwano Hiroyuki、Nishiyama Hiroyuki、Ishioka Chikashi、Takaishi Hiromasa、Shimodaira Hideki、Mogi Akira、Ando Yuichi、Matsumoto Koji、Kadowaki Daisuke、Muto Satoru
    • Journal Title

      Clinical and Experimental Nephrology

      Volume: 22 Issue: 1 Pages: 210-244

    • DOI

      10.1007/s10157-017-1448-z

    • NAID

      120007134431

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Predictive factors for pathological response in patients receiving neoadjuvant chemotherapy prior to radical cystectomy2018

    • Author(s)
      Ieda T, Muto S, Noma Y, Kanayama M, Koyasu H, Takahata S, China T, Kitamura K, Nagata M, Isotani S, Wakumoto Y, Horie S
    • Organizer
      16th Urological Association of Asia Congress 2018. 2018/4/17-21, Kyoto, Japan.
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Current strategy for the treatment of CRPC,2018

    • Author(s)
      堀江重郎
    • Organizer
      日台泌尿器腫瘍学会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ProstateCancer2018

    • Author(s)
      堀江重郎
    • Organizer
      APSU,The Catholic Univ. of Korea
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ,AR-V7 in Prostate Cancer2018

    • Author(s)
      堀江重郎
    • Organizer
      APPS2018,Grand Wailea
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Multidisciplinary Tumour Board session2018

    • Author(s)
      堀江重郎
    • Organizer
      ESMO Asia 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mini oral session of Genitourinary tumours,2018

    • Author(s)
      堀江重郎
    • Organizer
      ESMO Asia 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 泌尿器科領域におけるAIの未来とゲノム医療,2018

    • Author(s)
      堀江重郎
    • Organizer
      日本泌尿器科学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Precision Medicine 最前線2018

    • Author(s)
      堀江重郎
    • Organizer
      第18回日本抗加齢医学会総会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 前立腺がんのパラダイムシフト2018

    • Author(s)
      堀江重郎
    • Organizer
      第24回日本泌尿器科CPC
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] The expression of PSMA in CTCs as a predictive marker for poor treatment response in CRPC2018

    • Author(s)
      長屋直哉、金山麻裕子、永田政義、堀江重郎
    • Organizer
      GAP 2018 Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Circulating tumor cell in hormone-naive metastatic prostate cancer2017

    • Author(s)
      金山麻裕子、長屋直哉、永田政義、堀江重郎
    • Organizer
      ESMO Asia 2017 Congress、シンガポール
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The expression of prostate-specific membrane antigen in circulating tumor cells (CTC) of castration-resistant prostate cancer: The association with poor treatment response2017

    • Author(s)
      長屋直哉、金山麻裕子、永田政義、堀江重郎
    • Organizer
      ESMO Asia 2017 Congress、シンガポール
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research

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Published: 2017-11-13   Modified: 2024-03-26  

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