Comprehensive Studies toward Synthesis of Glycoproteins

• Project/Area Number: 17GS0420
• Research Category: Grant-in-Aid for Creative Scientific Research
• Allocation Type: Single-year Grants
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: ITO Yukishige The Institute of Physical and Chemical Research, 基幹研究所・細胞制御化学研究室, 主任研究員 (80168385)
• Co-Investigator(Kenkyū-buntansha): NAKAHARA Yoshiaki 東海大学, 工学部・生命化学科, 教授 (50087574) ; KAJIHARA Yasuhiro 大阪大学, 大学院・理学研究科・化学専攻, 教授 (50275020)
• Project Period (FY): 2005 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥343,590,000 (Direct Cost: ¥264,300,000、Indirect Cost: ¥79,290,000); Fiscal Year 2009: ¥54,990,000 (Direct Cost: ¥42,300,000、Indirect Cost: ¥12,690,000); Fiscal Year 2008: ¥68,510,000 (Direct Cost: ¥52,700,000、Indirect Cost: ¥15,810,000); Fiscal Year 2007: ¥70,330,000 (Direct Cost: ¥54,100,000、Indirect Cost: ¥16,230,000); Fiscal Year 2006: ¥73,710,000 (Direct Cost: ¥56,700,000、Indirect Cost: ¥17,010,000); Fiscal Year 2005: ¥76,050,000 (Direct Cost: ¥58,500,000、Indirect Cost: ¥17,550,000)
• Keywords: 糖タンパク質 / 糖鎖 / 細胞内プロセシング / 化学合成 / チオエステル法 / エリスロポエチン / 小胞体 / プロセシング / チオエステル / 高圧反応 / フォールディング / ライゲーション / 蛍光ラベル / マクロ分子クラウディング / 糖鎖ペプチドライブラリー / 高マンノース型糖鎖 / グリコシル化反応 / 立体選択性 / 複合型糖鎖 / ライブラリ合成 / N-アセチルラクトサミン / 0-結合型糖鎖 / ペプチドチオエステル / アスパラギン結合型糖鎖 / 高マンノース型 / ムチン型 / 複合型 / 固相合成 / シアリル糖鎖

Research Abstract

・Analysis of intracellular glycoprotein processing was conducted by using synthetic high-mannose-type glycans and their derivatives.
・Thioester forming reaction and amino-protecting group were developed and applied to syntheses of glycoproteins and dendrimers. A novel efficient reaction which utilize an ultra-high pressure was developed.
・Syntheses of glycoproteins which carry complex-type glycans were achieved. The most significant among them is the synthesis of erythmpoietin (EPO), a medically important glycoprotein. In vitro assay clarified that its cell-proliferating activity was almost indistinguishable with native EPO.

Research Products

• [Journal Article] The Mercaptomethyl Group Facilitates an Efficient One-Pot Ligation at Xaa-Ser/Thr for (Glyco) peptide Synthesis (2010)
  • Author(s): Hojo H. Ozawa C., Katayama H., Ueki A., Nakahara Y. Nakahara Y.
  • Journal Title: Angew.Chem.Int.Ed. 49 Pages: 5318-5321
• [Journal Article] Correct Disulfide Pairing Is Required for the Biological Activity of Crustacean Androgenic Gland Hormone (AGH) : Synthetic Studies of AGH (2010)
  • Author(s): Katayama H., Hojo H., Ohira T., Ishii A., Nozaki T., Goto K., Nakahara Y., Takahashi T., Hasegawa Y., Nagasawa H., Nakahara Y.
  • Journal Title: Biochemistry 49 Pages: 1798-1807
• [Journal Article] High-pressure-promoted Fmoc-aminoacylation of N-ethylcysteine : Preparation of key devices for the solid-phase synthesis of peptide thioesters (2010)
  • Author(s): Nakahara Y., Matsuo I., Ito Y., Ubagai R., Hojo, H., Nakahara Y.
  • Journal Title: Tetrahedron Lett 51 Pages: 407-410
• [Journal Article] Definitive evidence that a single N-glycan among three glycans on inducible costimulator is required for proper protein trafficking and ligand binding (2010)
  • Author(s): Naoki Kamei
  • Journal Title: Biochem.Biophys.Res.Commun. 391 Pages: 557-563
  • Peer Reviewed
• [Journal Article] Genetic analysis of glucosidase II β-subunit in trimming of high-mannose-type glycans (2009)
  • Author(s): Watanabe T., Totani K., Matsuo I., Maruyama J., Kitamoto K., Ito Y.
  • Journal Title: Glycobiology 19 Pages: 834-840
• [Journal Article] Synthesis of undecaprenyl pyrophosphate-linked glycans as donor substrates for bacterial protein N-glycosylation (2009)
  • Author(s): Lee Y.-J., Ishiwata A., Ito Y.
  • Journal Title: Tetrahedron 65 Pages: 6310-6319
• [Journal Article] Design and Synthesis of Homogeneous Erythropoietin Analogue with Two Human Complex-Type Sialyloligosaccharides (2009)
  • Author(s): Hirano K., Macmillan D., Tezuka K., Tsuji T., Kajihara Y.
  • Journal Title: Angew.Chem.Int.Ed. 48 Pages: 9557-9560
• [Journal Article] The recognition motif of the glycoprotein-folding sensor enzyme UDP-Glc : Glycoprotein glucosyltransferase (2009)
  • Author(s): Kiichiro Totani
  • Journal Title: Biochemistry 48 Pages: 2933-2940
  • Peer Reviewed
• [Journal Article] Genetic analysis of glucosidase II β-subunit in trimming of high-mannose-type glycans (2009)
  • Author(s): Taisuke Watanabe
  • Journal Title: Glycobiology 19 Pages: 834-840
  • Peer Reviewed
• [Journal Article] Synthesis of undecaprenyl pyrophosphate-linked glycans as donor substrates for bacterial protein N-glycosylation (2009)
  • Author(s): Yong Joo Lee
  • Journal Title: Tetrahedron 65 Pages: 6310-6319
  • Peer Reviewed
• [Journal Article] Establishment of a real-time analytical method for free oligosaccharide transport from the ER to the cytosol (2009)
  • Author(s): Yoshimi Haga
  • Journal Title: Glycobiology 19 Pages: 987-994
  • Peer Reviewed
• [Journal Article] Sugar-binding activity of the MRH domain in the ER α-glucosidase II β subunit is important for efficient glucose trimming (2009)
  • Author(s): Dan Hu
  • Journal Title: Glycobiology 19 Pages: 1127-1135
  • Peer Reviewed
• [Journal Article] Mechanism by which the lectin actinohivin blocks HIV infection of target cells (2009)
  • Author(s): Haruo Tanaka
  • Journal Title: PNAS 106 Pages: 15633-15638
  • Peer Reviewed
• [Journal Article] Solid-phase synthesis of O-sulfated glycopeptide by the benzyl-protected glycan strategy (2009)
  • Author(s): Keita Kawahira
  • Journal Title: Tetrahedron 65 Pages: 8143-8153
  • Peer Reviewed
• [Journal Article] High-pressure-promoted Fmoc-aminoacylation of N-ethylcysteine : Preparation of key devices for the solid-phase synthesis of peptide thioesters (2009)
  • Author(s): Yuko Nakahara
  • Journal Title: Tetrahedron Lett 51 Pages: 407-410
  • Peer Reviewed
• [Journal Article] Solid-phase synthesis of glycopeptide carrying a tetra-N-acetyllactosamine-containing core 2 decasaccharide (2009)
  • Author(s): Akiharu Ueki
  • Journal Title: Tetrahedron 66 Pages: 1742-1759
  • Peer Reviewed
• [Journal Article] Design and Synthesis of Homogeneous Erythropoietin Analogue with Two Human Complex-Type Sialyloligosaccharides (2009)
  • Author(s): Kiriko Hirano
  • Journal Title: Angew.Chem.Int.Ed. 48 Pages: 9557-9560
  • Peer Reviewed
• [Journal Article] Efficient Substitution Reaction from Cysteine to the Serine Residue of Glycosylated Polypeptide : Repetitive Peptide Segment Ligation Strategy and the Synthesis of Glycosylated Tetracontapeptide Having Acid LabileSialyl-T_N Antigens (2009)
  • Author(s): Ryo Okamoto
  • Journal Title: J.Org.Chem 74 Pages: 2494-2501
  • Peer Reviewed
• [Journal Article] Efficient and Systematic Synthesis of A Small Glycoconjugate Library Having Human Complex Type Oligosaccharides (2009)
  • Author(s): Takefumi Murase
  • Journal Title: Carbohydr.Res 344 Pages: 762-770
  • Peer Reviewed
• [Journal Article] Systematic synthesis and inhibitory activity of haloace tamidyl oligosaccharide derivatives toward cytoplasmic peptide: N-glycanase (2009)
  • Author(s): Ayako Miyazaki
  • Journal Title: Glycoconj. J 26 Pages: 133-140
  • Peer Reviewed
• [Journal Article] Structural and mutational studies on the importance of oligosaccharide binding for the activity of yeast PNGase (2009)
  • Author(s): Gang Zhao
  • Journal Title: Glycobiology 19 Pages: 118-125
  • Peer Reviewed
• [Journal Article] Pyruvoyl, a novel amino protecting group on the solid phase peptide synthesis and the peptide condensation reaction (2009)
  • Author(s): Hidekazu Katayama
  • Journal Title: Tetrahedron. Lett. 50 Pages: 818-821
  • Peer Reviewed
• [Journal Article] Efficient substitution reaction from cysteine to serine residue of glycosylated polypeptide: repetitive peptide segment ligation strategy and the synthesis of glycosylated tetracontapeptide having acid labile sialyl-TN antigens (2009)
  • Author(s): Ryo Okamoto
  • Journal Title: Journal of Organic Chemistry 74 Pages: 2494-2501
  • Peer Reviewed
• [Journal Article] Efficient and systematic synthesis of a small glycoconjugate library having human complex type oligosaccharides (2009)
  • Author(s): Takefumi Murase
  • Journal Title: Carbohydr. Res 344 Pages: 762-770
  • Peer Reviewed
• [Journal Article] NAP Ether-mediated intramolecular aglycon delivery : a unified strategy for 1, 2-cis glycosylation (2008)
  • Author(s): Ishiwata A., Munemura Y., Ito Y.
  • Journal Title: Eur.J.Org.Chem. 25 Pages: 4250-4263
• [Journal Article] Stereoselective synthesis of β-L-Rhamnopyranoside (2008)
  • Author(s): Lee Y.J., Ishiwata A., Ito Y.
  • Journal Title: J.Am.Chem.Soc. 130(20) Pages: 6330-6331
• [Journal Article] Effects of Macromolecular crowding on glycoprotein processing enzymes (2008)
  • Author(s): Totani K., Ihara Y., Matsuo I., Ito Y.
  • Journal Title: J.Am.Chem.Soc. 130 Pages: 2101-2107
• [Journal Article] Application of a novel thioesterification reaction to the synthesis of chemokine CCL27 by the modified thioester method. (2008)
  • Author(s): Hojo H, Murasawa Y., Katayama H., Ohira T., Nakahara, Y., Nakahara, Y.
  • Journal Title: Org.Biomol.Chem. 6 Pages: 1808-1813
• [Journal Article] Efficient sequential segment coupling using N-alkylcystein-assisted thioesterification for glycopeptides dendrimer synthesis (2008)
  • Author(s): Ozawa C., Katayama H., Hojo H., Nakahara Y., Nakahara Y.
  • Journal Title: Org.Lett. 10 Pages: 3531-3533
• [Journal Article] Chemical synthesis of a glycoprotein having an intact human complex-type sialyloligosaccharide under the Boc and Fmoc synthetic strategies (2008)
  • Author(s): Yamamoto N., Tanabe Y., Okamoto R., Dawson P.E., Kaiihara Y.
  • Journal Title: J.Am.Chem.Soc. 130 Pages: 501-510
• [Journal Article] The first synthesis of N-Man-Trp: Alternative mannosylation modification of protein (2008)
  • Author(s): Shino Manabe
  • Journal Title: SYNLETT Pages: 880-882
  • Peer Reviewed
• [Journal Article] Stereoselective synthesis of β-L-rhamnopyranosides (2008)
  • Author(s): Yong Joo Lee
  • Journal Title: J. Am. Chem. Soc. 130 Pages: 6330-6331
  • Peer Reviewed
• [Journal Article] Reductive Deprotection of Propargyl Ether by a SmI_2-amine-water System and Its Application to Polymer-supported Oligosaccharide Synthesis (2008)
  • Author(s): Shino Manabe
  • Journal Title: Tetrahedron Lett 49 Pages: 5159-5161
  • Peer Reviewed
• [Journal Article] NAP ether mediated intramolecular aglycon delivery: A unified strategy for 1, 2-cis glycosylation (2008)
  • Author(s): Akihiro Ishiwata
  • Journal Title: Eur. J. Org. Chem 26 Pages: 4250-4263
  • Peer Reviewed
• [Journal Article] Application of a novel thioesterification reaction to the synthesis of chemokine CCL27 by the modified thioester method (2008)
  • Author(s): Hironobu Hojo
  • Journal Title: Org. Biomol. Chem. 6 Pages: 1808-1813
  • Peer Reviewed
• [Journal Article] Efficient sequential segment coupling using N-alkylcystein-assisted thioesterification for glycopeptides dendrimer synthesis (2008)
  • Author(s): Chinatsu Ozawa
  • Journal Title: Org. Lett. 10 Pages: 3531-3533
  • Peer Reviewed
• [Journal Article] An efficient peptide ligation using azido-protected peptides via the thioester method (2008)
  • Author(s): Hidekazu Katayama
  • Journal Title: Tetrahedron. Lett. 49 Pages: 5492-5494
  • Peer Reviewed
• [Journal Article] Efficient Synthesis of MUC4 Sialylglycopeptide through the New Sialylation Using 5-Acetamido-Neuraminamide Donors (2008)
  • Author(s): Ryo Okamoto
  • Journal Title: Journal of Organic Chemistry 73 Pages: 3460-3466
  • Peer Reviewed
• [Journal Article] Uncovering latent ligation site for glycopeptide synthesis (2008)
  • Author(s): Okamoto Ryo
  • Journal Title: Angew. Chem. Int. Ed 47 Pages: 5402-5406
  • Peer Reviewed
• [Journal Article] The study of glycopeptide syntheses: exploring concise 0-linked sialylglycopeptide synthesis and glycopeptide coupling reaction (2008)
  • Author(s): Okamoto Ryo
  • Journal Title: Trends in Glycoscience and Glycotechnology 20 Pages: 203-217
  • Peer Reviewed
• [Journal Article] 糖鎖および糖ペプチドの化学合成研究II (2008)
  • Author(s): 中原義昭
  • Journal Title: 東海大学糖鎖工学研究施設紀要 2007 2 Pages: 34-37
• [Journal Article] (2008)
  • Author(s): Totani Kiichiro
  • Journal Title: Glycoscience 2^<nd> Editon, Chemistry and Chemical Biology Vol. 2(Monosaccharides as chiral auxiliaries and ligands for asymmetric synthesis) Pages: 1031-1075
• [Journal Article] (2008)
  • Author(s): Akihiro Ishiwata
  • Journal Title: Glycoscience 2^<nd> Editon, Chemistry and Chemical Biology Vol. 2(Stereoselective synthesis of β-mannno-glycosides) Pages: 1280-1312
• [Journal Article] (2008)
  • Author(s): Yukishige Ito
  • Journal Title: Rapid synthesis of oligosaccharides: Resin capture-release strategy Pages: 210-216
• [Journal Article] (2008)
  • Author(s): 伊藤幸成
  • Journal Title: 医薬応用に向けた糖鎖合成技術の活用1)有機合成化学を基盤とする糖タンパク質プロセシングと品質管理機構の解析(遺伝子医学MOOK11, 臨床糖鎖バイオマーカーの開発-糖鎖機能の解明とその応用) Pages: 88-94
• [Journal Article] (2008)
  • Author(s): Yoshiaki Nakahara
  • Journal Title: Experimental Glycoscience(Springer) Pages: 195-199
• [Journal Article] (2008)
  • Author(s): 北條裕信
  • Journal Title: 臨床糖鎖バイオマーカーの開発(メディカルドゥ) Pages: 115-119
• [Journal Article] Synergistic solvent effect in 1,2-cis-glycoside formation (2008)
  • Author(s): Akihiro Ishiwata
  • Journal Title: Tetrahedron 64 Pages: 92-102
  • Peer Reviewed
• [Journal Article] Effects of Macromolecular crowding on glycoprotein processing enzymes (2008)
  • Author(s): Kiichiro Totani
  • Journal Title: J. Am. Chem. Soc. 130 Pages: 2101-2107
  • Peer Reviewed
• [Journal Article] Stereoselective synthesis of benzyl-protected β-galactosides by propionitrile-mediated glycosylation. (2008)
  • Author(s): Akiharu Ueki
  • Journal Title: Tetrahedron 64 Pages: 2611-2618
  • Peer Reviewed
• [Journal Article] Chemical Synthesis of a Glycoprotein Having an Intact Human Complex-Type Sialyloligosaccharide under the Boc and Fmoc Synthetic Strategies. (2008)
  • Author(s): Naoki Yamamoto
  • Journal Title: J. Am. Chem. Soc. 130(2) Pages: 501-510
  • Peer Reviewed
• [Journal Article] Analysis of ER-associated glycoprotein degradation using synthetic glycopeptide probes (2007)
  • Author(s): Hagihara S., Goda K., Matsuo I., Ito Y.
  • Journal Title: Biochem.Biophys.Res.Commun. 360 Pages: 357-362
• [Journal Article] Synthesis of N-linked glycan derived from Gram-negative bacterium, Campylobacter jejuni (2007)
  • Author(s): Amin M.N., Ishiwata A., Ito Y.
  • Journal Title: Tetrahedron 63 Pages: 8181-8198
• [Journal Article] Synthesis of a natural oligosaccharide antibiotic active against Helicobacter pylori (2007)
  • Author(s): Manabe S., Ishii K., Ito Y.
  • Journal Title: J.Org.Chem. 72 Pages: 6107-6115
• [Journal Article] Fluorescently labeled inhibitor for profiling cytoplasmic peptide : N-glycanase (2007)
  • Author(s): Hagihara S., Miyazaki A., Matsuo I., Tatami A., Suzuki T., Ito Y.
  • Journal Title: Glycobiology 17 Pages: 1070-1076
  • NAID: 120006385865
• [Journal Article] An approach for a synthesis of asparagine-linked sialylglycopeptides having intact and homogeneous complex-type undecadisialyloligosaccharides (2007)
  • Author(s): Yamamoto N., Takayanagi A., Yoshino A., Sakakibara T., Kajihara Y.
  • Journal Title: Chem.Eur.J. 13 Pages: 613-625
• [Journal Article] Synthesis of biantennary LacNAc-linked O-glycan (core 4) and glycopeptide thioester by benzyl protection strategy : Rapid zinc reduction of G1cNTCA to GIcNAc by microwave irradiation (2007)
  • Author(s): Ueki A., Nakahara Y., Hojo H., Nakahara Y.
  • Journal Title: Tetrahedron 63 Pages: 2170-2181
• [Journal Article] N-Alkyl cystein-assisted thioesterification of peptides (2007)
  • Author(s): Hojo H., Onuma Y., Akimoto Y., Nakahara Y., Nakahara Y.
  • Journal Title: Tetrahedron Lett. 48 Pages: 25-28
• [Journal Article] An approach for a synthesis of asparagine-linked sialylglycopeptides having intact and homogeneous complex-type undecadisialyloligosaccharides (2007)
  • Author(s): Yamamoto N., Takayanagi A., Yoshino A., Sakakibara T., Kajihara Y.
  • Journal Title: Chem.Eur.J. 13(2) Pages: 613-625
• [Journal Article] Synthesis of complex-type glycans derived from parasitic helminths (2007)
  • Author(s): Jun Nakano
  • Journal Title: Carbohydr. Res. 342 Pages: 675-695
  • Peer Reviewed
• [Journal Article] Analysis of ER-associated glycoprotein degradation using syntheticglycopeptide probes (2007)
  • Author(s): Shinya Hagihara
  • Journal Title: Biochem. Biophys. Res. Commun. 360 Pages: 357-362
  • Peer Reviewed
• [Journal Article] Synthesis of N-linked glycan derived from Gram-negative bacterium, Campylobacter jejuni (2007)
  • Author(s): Mohammed Nurul Amin
  • Journal Title: Tetrahedron 63 Pages: 8181-8198
  • Peer Reviewed
• [Journal Article] Synthesis of a natural oligosaccharide antibiotic active against Helicobacter pylori (2007)
  • Author(s): Shino Manabe
  • Journal Title: J. Org. Chem. 72 Pages: 6107-6115
  • Peer Reviewed
• [Journal Article] Analyses of carbohydrate binding property of lectin-chaperone calreticulin (2007)
  • Author(s): Atsushi Tatami
  • Journal Title: Biochem. Biophys. Res. Commun. 364 Pages: 332-337
  • Peer Reviewed
• [Journal Article] Identification and characterization of an intracellular lectin, calnexin, from Aspergillus oryzae using N-glycan conjugated beads (2007)
  • Author(s): Taisuke Watanabe
  • Journal Title: Biosci. Biotechnol. Biochem. 71 Pages: 2688-2696
  • Peer Reviewed
• [Journal Article] Fluorescently labeled inhibitor for profiling cytoplasmic peptide: N-glycanase (2007)
  • Author(s): Shinya Hagihara
  • Journal Title: Glycobiology 17 Pages: 1070-1076
  • Peer Reviewed
• [Journal Article] Synthesis of glycopeptide dendrimer by a convergent method (2007)
  • Author(s): Chinatsu Ozawa
  • Journal Title: Tetrahedron 63 Pages: 9685-9690
  • Peer Reviewed
• [Journal Article] Recent progress in the field of glycopeptide synthesis (2007)
  • Author(s): Hirohobu Hojo
  • Journal Title: Biopolymer(peptide science) 88 Pages: 308-324
  • Peer Reviewed
• [Journal Article] Peptide thioester synthesis via an auxiliary-mediated N-S acyl shift reaction in solution (2007)
  • Author(s): Kenichiroh Nakamura
  • Journal Title: Int. J. Pept. Res. Ther 13 Pages: 191-202
  • Peer Reviewed
• [Journal Article] Purification and substrate specificity of UDP-D-xylose: β-D-glucose α-1,3-D-xylosyltransferase involved in the biosynthesis of the Xylα1-3Xylα1-3Glcβ1-O-Ser on EGF-like domains (2007)
  • Author(s): Takeshi Ishimizu
  • Journal Title: J. Biochem. 141 Pages: 593-600
  • Peer Reviewed
• [Journal Article] 糖鎖および糖ペプチドの化学合成研究 (2007)
  • Author(s): 中原義昭
  • Journal Title: 東海大学糖鎖工学研究施設紀要 2006 1 Pages: 24-26
• [Journal Article] An approach for a synthesis of asparagine-linked sialylglycopeptides having intact and homogeneous complex-type undecadisialyloligosaccharides (2007)
  • Author(s): Naoki Yamamoto
  • Journal Title: Chem. Eur. J. 13(2) Pages: 613-625
  • Peer Reviewed
• [Journal Article] Synthesis of CMP-9"-modified-sialic acids as donor substrate analogues for mammalian and bacterial sialyltransferases (2007)
  • Author(s): Yasuhiro Kajihara
  • Journal Title: Carbohydr. Res. 342(12-13) Pages: 1680-1688
  • Peer Reviewed
• [Journal Article] Virtually epimerization-free synthesis of peptide-α-thio-esters (2007)
  • Author(s): Tyrone J. Hogenauer
  • Journal Title: Org. Biomol. Chem. 5(5) Pages: 759-762
  • Peer Reviewed
• [Journal Article] Solid-state 17O NMR in biological solids: efficient 17O labeling of amino acids and peptides (2007)
  • Author(s): Kazuhiko Yamada
  • Journal Title: Chem. Lett. 36(1) Pages: 192-193
  • Peer Reviewed
• [Journal Article] N-Alkyl cystein-assisted thioesterification of peptides. (2007)
  • Author(s): H.Hojo, Y.Onuma, Y.Akimoto, Y.Nakahara, Y.Nakahara
  • Journal Title: Tetrahedron Lett. 48 Pages: 25-28
• [Journal Article] Solid-phase synthesis of core 3 and core 6 O-glycan-linked glycopeptides by benzyl-protein method. (2007)
  • Author(s): Y.Nakahara, C.Ozawa, E.Tanaka, K.Ohtsuka, Y.Takano, H.Hojo, Y.Nakahara
  • Journal Title: Tetrahedron 63 Pages: 2161-2169
• [Journal Article] Synthesis of biantennary LacNAc-linked O-glycan (core 4) and glycopeptide thioester by benzyl protection strategy : Rapid zinc reduced of GlcNTCA to GlcNAc by microwave irradiation. (2007)
  • Author(s): A.Ueki, Y.Nakahara, H.Hojo, Y.Nakahara
  • Journal Title: Tetrahedron 63 Pages: 2170-2181
• [Journal Article] Solid-state 17O NMR in biological solids : Efficient 17O labeling of amino acids and peptides (2007)
  • Author(s): K.Yamada, T.Yamazaki, M.Asanuma, H.Hirota, N.Yamamoto, Y.Kajihara
  • Journal Title: Chem.Lett. 36 Pages: 192-193
  • NAID: 10018664115
• [Journal Article] Convenient synthesis of a sialylglycopeptide-thioester having an intact and homogeneous complex-type disialyl-oligosaccharide (2007)
  • Author(s): N.Yamamoto, A.Takayanagi, A.Yoshino, T.Sakakibara, Y.Kajihara
  • Journal Title: Carbohydr.Res. 341 Pages: 1333-1340
• [Journal Article] Virtually Epimerization-Free Synthesis of Peptide-alpha-Thioesters (2007)
  • Author(s): T.J.Hogenauer, Q.Wang, A.K.Sanki, A.J.Gammon, C.H.chu, C.M.Kaneshiro, Y.Kajihara, K.Michael
  • Journal Title: Org.Biomol.Chem. (in press)
• [Journal Article] Convenient synthesis of a sialylglycopeptide-thioester having an intact and homogeneous complex-type disialyl-oligosaccharide (2006)
  • Author(s): Kajihara Y Yoshihara A., Hirano K., Yamamoto N.
  • Journal Title: Carbohydr.Res. 341(10) Pages: 1333-1340
• [Journal Article] Highly efficient synthesis of sialylglycopeptides overcoming unexpected aspartimide formation during activation of Fmoc-Asn (sundecadisialyloligosaccharide)-OH (2006)
  • Author(s): Yamamoto P.E., Kajihara. Y.
  • Journal Title: Tetrahedron Lett. 47(8) Pages: 1341-1346
• [Journal Article] Substrate specificity analysis of endoplasmic reticulum glucosidase II using synthetic high mannose-type glycans (2006)
  • Author(s): Totani K., Ihara Y., Matsuo I., Ito Y.
  • Journal Title: J.Biol.Chem. 281 Pages: 31502-31508
• [Journal Article] Comprehensive synthesis of ER related high-mannose-type sugar chains by covergent strategy (2006)
  • Author(s): Matsuo I., Totani K., Tatami A., Ito Y.
  • Journal Title: Tetrahedron 62 Pages: 8262-8277
• [Journal Article] Facile synthesis of oligosaccharide probes for the analysis of protein-carbohydrate interactions (2006)
  • Author(s): Takatani M., Ito Y.
  • Journal Title: Chem.Asian J. 1 Pages: 64-75
• [Journal Article] A stereoselective 1,2-cis glycosylation toward the synthesis of a novel N-linked glycan from the Gram-negative bacterium,Campylobacter jejuni (2006)
  • Author(s): A.Ishiwata, S.Ohta, Y.Ito
  • Journal Title: Bioorg.＆Med.Chem. 14 Pages: 3049-3061
• [Journal Article] Synthesis of asparagine-linked bacillosamine (2006)
  • Author(s): M.N.Amin, A.Ishiwata, Y.Ito
  • Journal Title: Carbohydr.Res. 341 Pages: 1922-1929
  • NAID: 120006385424
• [Journal Article] 有機合成化学による糖タンパク質糖鎖の機能解明 (2006)
  • Author(s): 戸谷希一郎, 萩原伸也, 伊藤幸成
  • Journal Title: 有機合成化学協会誌 64 Pages: 492-501
  • NAID: 10017486742
• [Journal Article] Systematic synthesis of bisubstrate-type inhibitors of N-acetylglucosaminyltransferases (2006)
  • Author(s): S.Hanashima, K.Inamori, S.Manabe, N.Taniguchi, Y.Ito
  • Journal Title: Chem.Eur.J. 12 Pages: 3449-3462
• [Journal Article] High-mannose-type glycan modifications of dihydrofolate reductase using glycan-methotrexate conjugates (2006)
  • Author(s): K.Totani, I.Matsuo, Y.Ihara, Y.Ito
  • Journal Title: Bioorg.Med.Chem. 14 Pages: 5220-5229
• [Journal Article] Comprehensive synthesis of ER related high-mannose-type sugar chains by covergent strategy (2006)
  • Author(s): I.matsuo, K.Totani, A.Tatami, Y.Ito
  • Journal Title: Tetrahedron 62 Pages: 8262-8277
• [Journal Article] Substrate specificity analysis of endoplasmic reticulum glucosidase II using synthetic high mannose-type glycans (2006)
  • Author(s): K.Totani, Y.Ihara, I.Matsuo, Y.Ito
  • Journal Title: J.Biol.Chem. 281 Pages: 31502-31508
• [Journal Article] Synthesis of a glycosylated peptide thioester by the Boc strategy and its application to segment condensation. (2006)
  • Author(s): E.Haginoya, H.Hojo, Y.Nakahara, Y.Nakahara, K.Nabeshima, B.P.Toole, Y.Watanebe
  • Journal Title: Biosci.Biotechnol.Biochem. 70 Pages: 1338-1349
  • NAID: 10018529890
• [Journal Article] Chemoenzymatic Synthesis of a MUC1 Glycopeptide Carring Non-natural Sialy1 TF-βO-Glcan. (2006)
  • Author(s): E.Tanaka, Y.Nakahara, Y.Kuroda, Y.Takano, N.Kojima, H.Hojo, Y.Nakahara.
  • Journal Title: Biosci.Biotechnol.Biochem. 70 Pages: 2515-2522
• [Journal Article] Efficient microwave-assisted tandem N- to S-acyl transfer and thioester exchange for the preparation of a glycosylated peptide thioester. (2006)
  • Author(s): F.Nagaike, Y.Onuma, C.Kanazawa, H.Hojo, A.Ueki, Y.Nakahara, Y.Nakahara
  • Journal Title: Org.Lett. 8 Pages: 4465-4468
• [Journal Article] Convenient synthesis of a sialylglycopeptide-thioester having an intact and homogeneous complex-type disialyl-oligosaccharide (2006)
  • Author(s): Y.Kajihara, A.Yoshihara, K.Hirano, N.Yamamoto
  • Journal Title: Carbohydr.Res. 341 Pages: 1333-1340
• [Journal Article] Synthesis of complex-type glycans derived from parasitic helminths. (2006)
  • Author(s): J.Nakano, H.Ohta, Y.Ito
  • Journal Title: Bioorg.Med.Chem.Lett. 16 Pages: 928-933
• [Journal Article] Highly efficient synthesis of sialylglycopeptides overcoming unexpected aspartimide formation during activation of Fmoc-Asn(undecadisilalyloligosaccharide)-OH. (2006)
  • Author(s): N.Yamamoto, A.Takayanagi, T.Sakakibara, P.E.Dawson, Y.Kajihara
  • Journal Title: Tetrahedron Letters 47(8) Pages: 1341-1346
• [Journal Article] Design and synthesis of oligosaccharides that interfere with glycoprotein quality-control systems (2005)
  • Author(s): Arai M.A., Matsuo I., Hagihara S., Totani K., Maruyama J.-i., Kitamoto K., Ito Y.
  • Journal Title: Chem Bio Chem. 6 Pages: 2281-2289
• [Journal Article] Synthetic substrates for an endoplasmic reticulum protein-folding sensor, UDP-glucose : Glycoprotein glucosyltransferase (2005)
  • Author(s): Totani K., Ihara Y., Matsuo I., Koshino H., Ito Y.
  • Journal Title: Angew.Chem.Int.Ed. 44 Pages: 7950-7954
• [Journal Article] First chemical synthesis of triglucosylated tetradecasaccharide (Glc3Man9GlcNAc2), a common precursor of asparagine-linked oligosaccharides (2005)
  • Author(s): Matsuo I., Kashiwagi T., Totani K., Ito Y.
  • Journal Title: Tetrahedron Lett. 46 Pages: 4197-4200
• [Journal Article] Thermodynamic analysis of interactions between N-linked sugar chains and F-box protein Fbsl (2005)
  • Author(s): Hagihara S., Totani K., Matsuo I., Ito Y.
  • Journal Title: J.Med.Chem. 48 Pages: 3126-3129
• [Journal Article] Chemical Synthesis of 23 kDa Glycoprotein by Repetitive Segment Condensation : A Synthesis of MUC2 Basal Motif Carrying Multiple O-GalNAc Moieties (2005)
  • Author(s): Hojo H., Matsumoto Y., Nakahara Y., Ito E., Suzuki Y., Suzuki M., Suzuki A., Nakahara Y.
  • Journal Title: J.Am.Chem.Soc. 127 Pages: 13720-13725
• [Journal Article] High throughput screening of O-glycosylation conditions. (2005)
  • Author(s): A.Ishiwata, Y.Ito
  • Journal Title: Tetrahedron Lett. 46 Pages: 3521-3524
• [Journal Article] Thermodynamic analysis of interactions between N-linked sugar chains and F-box protein Fbs1. (2005)
  • Author(s): S.Hagihara, K.Totani, I.Matsuo, Y.Ito
  • Journal Title: J.Med.Chem. 48 Pages: 3126-3129
• [Journal Article] First chemical synthesis of triglucosylated tetradecasaccharide (Gle3Man9GlcNAc2), a common precursor of asparagine-linked oligosaccharides. (2005)
  • Author(s): I.Matsuo, T.Kashiwagi, K.Totani, Y.Ito
  • Journal Title: Tetrahedron Lett. 46 Pages: 4197-4200
• [Journal Article] Divergent synthesis of sialylated glycan chains : Combined use of polymer support, resin capture-release, and chemoenzymatic strategies. (2005)
  • Author(s): S.Hanashima, S.Manaba, Y.Ito
  • Journal Title: Angew.Chem.Int.Ed. 44 Pages: 4218-4224
• [Journal Article] Systhematic synthesis of ER related N-glycans using convergent strategy. (2005)
  • Author(s): I.Matsuo, Y.Ito
  • Journal Title: Trends in glycoscience and glycotechnology 17 Pages: 85-95
• [Journal Article] Synthesis and application of novel sugar chain molecular probe Glycan-MTX. (2005)
  • Author(s): K.Totani, Y.Ito
  • Journal Title: Trends in glycoscience and glycotechnology 17 Pages: 121-130
  • NAID: 10016432805
• [Journal Article] Synthetic substrates for an endoplasmic reticulum protein-folding sensor, UDP-glucose : Glycoprotein glucosyltransferase. (2005)
  • Author(s): K.Totani, Y.Ihara, I.Matsuo, H.Koshino, Y.Ito
  • Journal Title: Angew.Chem.Int.Ed. 44 Pages: 7950-7954
• [Journal Article] Design and synthesis of oligosaccharides that interfere with glycoprotein quality-control systems. (2005)
  • Author(s): M.A.Arai, I.Matsuo, S.Hagahara, K.Totani, J.-i.Maruyama, K.Kitamoto, Y.Ito
  • Journal Title: ChemBioChem. 6 Pages: 2281-2289
• [Journal Article] Structural approaches to the study of oligosaccharides in glycoprotein quality control. (2005)
  • Author(s): Y.Ito, S.Hagihara, I.Matsuo, K.Totani
  • Journal Title: Current Opinion in Structural Biology 15 Pages: 481-489
• [Journal Article] Increased expression of protein C-mannosylation in the aortic vessels of siabetic Zucker rats. (2005)
  • Author(s): Y.Ihara, S.Manabe, M.Kanda, H.Kawano, T.Nakayama, I.Sekine, T.Kondo, Y.Ito
  • Journal Title: Glycobiology 15 Pages: 383-392
• [Journal Article] Chemical Synthesis of 23 kDa Glycoprotein by Repetitive Segment Condensation : A Synthesis of MUC2 Basal Motif Carrying Multiple O-GalNAc Moieties (2005)
  • Author(s): H.Hojo, Y.Matsumoto, Y.Nakahara, E.Ito, Y.Suzuki, M.Suzuki, A.Suzuki, Y.Nakahara
  • Journal Title: J.Am.Chem.Soc 127 Pages: 13720-13725
• [Journal Article] Solid-phase Synthesis of Core 8 O-Glycan-Linked MUC5AC Glycopeptide (2005)
  • Author(s): M.Maemura, A.Ohgaki, Y.Nakahara, H.Hojo, Y.Nakahara
  • Journal Title: Biosci.Biotechnol.Biochem 69 Pages: 1575-1583
  • NAID: 130000030246
• [Journal Article] Combinatorial Synthesis of MUC1 Glycopeptides : Polymer Blotting Facilitates Chemical and Enzymatic Synthesis of Highly Complicated Mucin Glycopeptides (2005)
  • Author(s): M.Fumoto, H.Hinou, T.Ohta, T.Ito, K.Yamada, A.Takimoto, H.Kondo, H.Shimizu, T.Inazu, Y.Nakahara, S.Nishimura
  • Journal Title: J.Am.Chem.Soc 127 Pages: 11804-11818
• [Journal Article] Molecular-weight-tagged glycopeptide library : Efficient construction and applications. (2005)
  • Author(s): H.Ito, A.Kameyama, T.Sato, K.Kiyohara, Y.Nakahara, H.Narumatsu
  • Journal Title: Angew.Chem.Int.Ed.. 44 Pages: 4547-4549
• [Journal Article] Efficient and Unambiguous assignment method combined with selective-TOCSY-HSQC, selective-TOCSY-DQFCOSY and selective-TOCSY-NOESY experiments for naturally occurring large oligosaccharides (2005)
  • Author(s): H.Sato, Y.Kajihara
  • Journal Title: Carbohydrate Research 340(3) Pages: 469-479
• [Presentation] 合成化学を基盤とする糖タンパク質の細胞内機能解析 (2010)
  • Author(s): 伊藤幸成
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 大阪府、近畿大学本部キャンパス
  • Year and Date: 2010-03-29
• [Presentation] 凍結反応条件下でのグリコシル化反応における基質濃度及び糖供与体保護基の影響 (2010)
  • Author(s): 櫻井絢花
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 大阪府、近畿大学本部キャンパス
  • Year and Date: 2010-03-29
• [Presentation] 麹菌A.oryxae の小胞体関連分解(ERAD)因子AoHtm1, AoYos9の機能解析 (2010)
  • Author(s): 菊間隆志
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京都、東京大学安田講堂
  • Year and Date: 2010-03-29
• [Presentation] 結核菌由来アラビナン鎖状9糖フラグメントの合成 (2010)
  • Author(s): 石渡明弘
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 大阪府、近畿大学本部キャンパス
  • Year and Date: 2010-03-28
• [Presentation] 麹菌における糖タンパク質品質管理機構関連分子の機能解析 (2010)
  • Author(s): 渡邉泰祐
  • Organizer: 日本農芸化学会2010年度大会
  • Place of Presentation: 東京都・東京大学安田講堂
  • Year and Date: 2010-03-28
• [Presentation] 合成糖鎖を用いる新規小胞体タンパク質マレクチンの特異性解析 (2010)
  • Author(s): 武田陽一
  • Organizer: 日本化学会第90春季年会
  • Place of Presentation: 大阪府、近畿大学本部キャンパス
  • Year and Date: 2010-03-26
• [Presentation] 化学合成を基盤とする糖タンパク質品質管理機構の解析 (2010)
  • Author(s): 伊藤幸成
  • Organizer: 「糖鎖の生物機能の解明と利用技術」最終(公開)シンポジウム
  • Place of Presentation: 東京都、品川コクヨホール
  • Year and Date: 2010-01-20
• [Presentation] 合成化学に立脚する糖鎖研究 (2009)
  • Author(s): 伊藤幸成
  • Organizer: 第7回糖鎖科学コンソーシアムシンポジウム
  • Place of Presentation: 大阪府、千里ライフサイエンスセンター
  • Year and Date: 2009-12-07
• [Presentation] O-結合型糖鎖合成と糖タンパク質への展開 (2009)
  • Author(s): 中原義昭
  • Organizer: 第7回糖鎖科学コンソーシアムシンポジウム
  • Place of Presentation: 大阪府、千里ライフサイエンスセンター
  • Year and Date: 2009-12-07
• [Presentation] Synthetic high-mannose-type glycans for analyses of glucosidase II and UDP-Glc : glycoprotein glucosyltransferase (2009)
  • Author(s): 伊藤幸成
  • Organizer: 20th International Symposium on Glycoconjugates
  • Place of Presentation: San Juan, Puerto Rico
  • Year and Date: 2009-11-30
• [Presentation] 麹菌の糖タンパク質品質管理機構に関連する遺伝子破壊株を用いた糖鎖構造解析 (2009)
  • Author(s): 渡邉泰祐
  • Organizer: 第9回糸状菌分子生物学コンファレンス
  • Place of Presentation: 東京都、東京大学弥生講堂
  • Year and Date: 2009-11-18
• [Presentation] 麹菌A.oryzae における小胞体関連分解(ERAD)関連遺伝子 Aohtm1, Aoyos9の機能解析 (2009)
  • Author(s): 菊間隆志
  • Organizer: 第9回糸状菌分子生物学コンファレンス
  • Place of Presentation: 東京都・東京大学弥生講堂
  • Year and Date: 2009-11-18
• [Presentation] 凍結条件下におけるグリコシル化の立体選択性 (2009)
  • Author(s): 櫻井絢花
  • Organizer: Glyco TOKYO2009シンポジウム
  • Place of Presentation: 東京、御茶ノ水大学共通講義棟
  • Year and Date: 2009-11-14
• [Presentation] Synthesis of Key Components for Protein N- and O-Glycosylations in Campylobacter jejuni (2009)
  • Author(s): Yong Joo Lee
  • Organizer: The 11th International Kyoto Conference on New Aspects of Organic Chemistry
  • Place of Presentation: Kyoto, Rihga Royal Hotel Kyoto
  • Year and Date: 2009-11-11
• [Presentation] Synthetic high-mannose-type glycans for analyses of glycoprotein processing in the ER (2009)
  • Author(s): 伊藤幸成
  • Organizer: The 1st Asian Communications of Glycobiology and Glycotechnology Conference
  • Place of Presentation: Tsukuba, AIST Tukuba Center
  • Year and Date: 2009-10-29
• [Presentation] Synthesis of Homogeneous Glycoproteins Having Complex Type Oligosaccharides (2009)
  • Author(s): 梶原康宏
  • Organizer: The 1st Asian Communications of Glycobiology and Glycotechnology Conference
  • Place of Presentation: Tsukuba, AIST Tukuba Center
  • Year and Date: 2009-10-29
• [Presentation] TGFbシグナルのC-マンノシル化TSR由来ペプチドによる制御 (2009)
  • Author(s): 井原義人
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 兵庫県・神戸国際会議場
  • Year and Date: 2009-10-24
• [Presentation] ERGIC-35、MCFD2と血液凝固因子欠損症 (2009)
  • Author(s): 宮坂佳樹
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 兵庫県・神戸国際会議場
  • Year and Date: 2009-10-22
• [Presentation] Organic synthesis and glycobiology (2009)
  • Author(s): 伊藤幸成
  • Organizer: The 3rd International Symposium on Bio-Inspired Engineering
  • Place of Presentation: Taipei, Taiwan
  • Year and Date: 2009-10-21
• [Presentation] Synthesis of Homogeneous Glycoproteins Having Complex Type Oligosaccharides (2009)
  • Author(s): 梶原康宏
  • Organizer: Modern Solid Phase Peptide Synthesis and Its Application
  • Place of Presentation: Gold Coast, Australia
  • Year and Date: 2009-10-10
• [Presentation] 結核菌細胞壁成分アラビナンの22糖の合成 (2009)
  • Author(s): 石渡明弘
  • Organizer: 第51回天然有機化合物討論会
  • Place of Presentation: 愛知県、名古屋市公会堂
  • Year and Date: 2009-10-08
• [Presentation] 有機化学の視点でながめる小胞体内糖タンパク質の動態 (2009)
  • Author(s): 伊藤幸成
  • Organizer: 第29回生命科学シンポジウム「生物機能と有機化学-ケミカルバイオロジーの新展開-」
  • Place of Presentation: 千葉県、東邦大学習志野キャンパス
  • Year and Date: 2009-09-30
• [Presentation] 核菌由来アラビナン22糖モチーフの合成 (2009)
  • Author(s): 石渡明弘
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 岐阜県、飛騨・世界生活文化センター
  • Year and Date: 2009-09-11
• [Presentation] 新規収斂的ルートによるN-結合型糖アミノ酸の合成研究 (2009)
  • Author(s): 朝比奈雄也
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 岐阜県、飛騨・世界生活文化センター
  • Year and Date: 2009-09-11
• [Presentation] 糖ペプチド固相合成のための複合型糖アミノ酸ビルディングブロックの合成研究 (2009)
  • Author(s): 萩原匡
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 岐阜県、飛騨・世界生活文化センター
  • Year and Date: 2009-09-11
• [Presentation] タンパク質糖鎖の化学合成と生物有機化学 (2009)
  • Author(s): 伊藤幸成
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 岐阜県、飛騨・世界生活文化センター
  • Year and Date: 2009-09-10
• [Presentation] Syntheses of Substrates for Bacterial Protein N-Glycosylation Pathway (2009)
  • Author(s): 李龍柱
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 岐阜県、飛騨・世界生活文化センター
  • Year and Date: 2009-09-10
• [Presentation] 麹菌の糖タンパク質品質管理機構に関連する遺伝子破壊株を用いた糖鎖構造解析 (2009)
  • Author(s): 渡邉泰祐
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 岐阜県、飛騨・世界生活文化センター
  • Year and Date: 2009-09-10
• [Presentation] 凍結条件下におけるグリコシル化反応の濃度の効果について (2009)
  • Author(s): 櫻井絢花
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 岐阜県、飛騨・世界生活文化センター
  • Year and Date: 2009-09-10
• [Presentation] 疎水性基を持つ糖タンパク質様プローブによるグルコース転移酵素反応の解析 (2009)
  • Author(s): 宮川淳
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 岐阜県、飛騨・世界生活文化センター
  • Year and Date: 2009-09-10
• [Presentation] 精密有機合成法と生化学的なアプローチによる共刺激糖タンパク質AILIM/ICOSの糖鎖機能の解明研究 (2009)
  • Author(s): 梶原康宏
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 岐阜県、飛騨・世界生活文化センター
  • Year and Date: 2009-09-09
• [Presentation] C-マンノシル化TSR由来ペプチドによるTGFβシグナルの制御 (2009)
  • Author(s): 井原義人
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 岐阜県、飛騨・世界生活文化センター
  • Year and Date: 2009-09-09
• [Presentation] マレクチンの基質特異性解析とグルコシダーゼIIの活性に与える影響 (2009)
  • Author(s): 武田陽一
  • Organizer: 第29回日本糖質学会年会
  • Place of Presentation: 岐阜県、飛騨・世界生活文化センター
  • Year and Date: 2009-09-09
• [Presentation] Oligosaccharide synthesis for glycobiology (2009)
  • Author(s): 伊藤幸成
  • Organizer: 238th American Chemical Society National Meeting and Exposition
  • Place of Presentation: Washington, USA
  • Year and Date: 2009-08-17
• [Presentation] Evaluation of oligosaccharide effects by use of chemical synthesis of homogeneous glycoproteins having complex type oligosaccharides (2009)
  • Author(s): 梶原康宏
  • Organizer: 15th European Carbohydrate Symposium
  • Place of Presentation: Vienna, Austria
  • Year and Date: 2009-06-21
• [Presentation] 糖鎖生物学との融合をめざす合成研究 (2009)
  • Author(s): 伊藤幸成
  • Organizer: 第19回万有福岡シンポジウム
  • Place of Presentation: 福岡県、九州大学医学部百年講堂
  • Year and Date: 2009-05-23
• [Presentation] Stereoselective constructions of complex bacterial glycans (2009)
  • Author(s): 石渡明弘
  • Organizer: RIKEN-NTU-NUS Joint Seminar
  • Place of Presentation: Singapore, Singapore
  • Year and Date: 2009-05-20
• [Presentation] 有機化学の視点で糖タンパク質をしらべる (2009)
  • Author(s): 伊藤幸成
  • Organizer: 天然有機化合物討論会50周年記念講演会
  • Place of Presentation: 宮城県, 仙台国際センター
  • Year and Date: 2009-05-16
• [Presentation] Strategies toward stereoselective synthesis of 1,2-cis glycosides (2009)
  • Author(s): 石渡明弘
  • Organizer: A*STAR-RIKEN Joint Symposium 2009
  • Place of Presentation: Singapore, Singapore
• [Presentation] bromoconduritolによるグルコシダーゼII阻害機構の解析 (2009)
  • Author(s): 神戸
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
• [Presentation] UDP-グルコース類縁体を用いたグルコース転移酵素UGGTの基質特異性解析と糖転移生成物の小胞体グルコシダーゼIIによる切断活性評価 (2009)
  • Author(s): 宮川淳
  • Organizer: 日本化学会第89春季年会
  • Place of Presentation: 船橋
• [Presentation] 麹菌における糖タンパク質品質管理機構関連遺伝子破壊株の糖鎖構造解析 (2009)
  • Author(s): 渡邉泰祐
  • Organizer: 日本農芸化学会2009年度大会
  • Place of Presentation: 福岡
• [Presentation] 蛍光標識糖鎖を用いたカルネキシンサイクルの解析 (2008)
  • Author(s): 松尾一郎
  • Organizer: 第31回日本分子生物学会年会・第81回 日本生化学会大会(BMB2008)
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-09
• [Presentation] Analysis of glycoprotein processing with organic synthesis (2008)
  • Author(s): Yukishige Ito
  • Organizer: International Symposium on Systems Glycobiology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-12-05
• [Presentation] Synthesis of undecaprenyl pyrophosphate-linked glycans (2008)
  • Author(s): Yong Joo Lee
  • Organizer: International Symposium on Systems Glycobiology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-12-05
• [Presentation] Fluorescently labeled substrate for analysis of cytoplasmic peptide: N-glycanas (2008)
  • Author(s): Matsuo Ichiro
  • Organizer: International Symposium on Systems Glycobiology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-12-05
• [Presentation] Quantitative analysis of glycoprotein processing in the ER (2008)
  • Author(s): Kiichiro Totani
  • Organizer: International Symposium on Systems Glycobiology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-12-05
• [Presentation] Analysis of ER glycoprotein quality control system of aspergillus oryzae (2008)
  • Author(s): Taisuke Watanabe
  • Organizer: International Symposium on Systems Glycobiology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-12-05
• [Presentation] Strategies toward stereoselective synthesis of 1, 2-cis glycosides (2008)
  • Author(s): Akihiro Ishiwata
  • Organizer: International Symposium on Systems Glycobiology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-12-05
• [Presentation] Enzymatic transfer of UDP-glucose analogs by UGGT toward clarifying the donor specificity of UGGT (2008)
  • Author(s): Atsushi Miyagawa
  • Organizer: International Symposium on Systems Glycobiology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-12-05
• [Presentation] On the mechanism of glucosidase II inhibition by bromoconduritol (2008)
  • Author(s): Yoichi Takeda
  • Organizer: International Symposium on Systems Glycobiology
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2008-12-05
• [Presentation] N-結合型糖鎖プローブを用いた麹菌グルコシダーゼIIの解析 (2008)
  • Author(s): 渡邉泰祐
  • Organizer: 第8回糸状菌分子生物学コンファレンス
  • Place of Presentation: 金沢
  • Year and Date: 2008-11-18
• [Presentation] Chemical Synthesis of Small Glycoprotein (2008)
  • Author(s): Yasutaka Tanabe
  • Organizer: 第45回ペプチド討論会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-29
• [Presentation] Synthetic Study of Glycosylated Ovomucoid (2008)
  • Author(s): Shun Sasaoka
  • Organizer: 第45回ペプチド討論会
  • Place of Presentation: 東京
  • Year and Date: 2008-10-29
• [Presentation] 糖タンパク質折り畳みセンサー酵素UGGTの基質特異性解析 (2008)
  • Author(s): 戸谷希一郎
  • Organizer: 第50回天然有機化合物討論会
  • Place of Presentation: 福岡
  • Year and Date: 2008-10-02
• [Presentation] NAPエーテルを利用した分子内アグリコン転移反応の開発と複合糖質糖鎖合成への応用 (2008)
  • Author(s): 石渡明弘
  • Organizer: 第50回天然有機化合物討論会
  • Place of Presentation: 福岡
  • Year and Date: 2008-10-02
• [Presentation] 化学法と発現法を組み合わせた糖タンパク質エリスロポエチン誘道体の合成 (2008)
  • Author(s): 平野桐子
  • Organizer: 50回天然物討論会
  • Place of Presentation: 福岡
  • Year and Date: 2008-10-01
• [Presentation] Synthesis of the undecaprenyl pyrophosphate-linked glycans (2008)
  • Author(s): 李龍柱
  • Organizer: 第28回日本糖質学会年会
  • Place of Presentation: つくば
  • Year and Date: 2008-08-20
• [Presentation] 糖タンパク質フォールディングセンサーUGGTのドナー基質特異性解析 (2008)
  • Author(s): 宮川淳
  • Organizer: 第28回日本糖質学会年会
  • Place of Presentation: つくば
  • Year and Date: 2008-08-20
• [Presentation] ^<19>NMRによる糖鎖プローブとレクチンの相互作用解析 (2008)
  • Author(s): 武田陽一
  • Organizer: 第28回日本糖質学会年会
  • Place of Presentation: つくば
  • Year and Date: 2008-08-20
• [Presentation] 化学法と発現法を組み合わせたエリスロポエチン誘導体の合成研究 (2008)
  • Author(s): 平野桐子
  • Organizer: 第28回日本糖質学会
  • Place of Presentation: つくば
  • Year and Date: 2008-08-20
• [Presentation] 新規糖ペプチド鎖連結法の開発 (2008)
  • Author(s): 岡本亮
  • Organizer: 第28回日本糖質学会
  • Place of Presentation: つくば
  • Year and Date: 2008-08-20
• [Presentation] 合成基質を用いた糖タンパク質折り畳みセンサー酵素UGGTの特異性解析 (2008)
  • Author(s): 戸谷希一郎
  • Organizer: 第28回日本糖質学会年会
  • Place of Presentation: つくば
  • Year and Date: 2008-08-19
• [Presentation] N-結合型糖鎖プローブを用いた麹菌カルネキシンとグルコシダーゼIIの解析 (2008)
  • Author(s): 渡邉泰祐
  • Organizer: 第28回日本糖質学会年会
  • Place of Presentation: つくば
  • Year and Date: 2008-08-19
• [Presentation] クリコシル化の高効率立体制御法の開発と細菌由来糖鎖構築への応用 (2008)
  • Author(s): 石渡明弘
  • Organizer: 第28回日本糖質学会年会
  • Place of Presentation: つくば
  • Year and Date: 2008-08-18
• [Presentation] Strategies toward stereoselective synthes is of 1, 2-cis glycosideso (2008)
  • Author(s): Akihiro Ishiwata
  • Organizer: 24th International Carbohydrate Symposium
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2008-07-31
• [Presentation] Synthesis of erythropoietin analogue having two homogeneous complex type sialyloligosaccharides by use of combined chemical and expression methods (2008)
  • Author(s): Kiriko Hirano
  • Organizer: 24^<th> International Carbohydrate Synpojium
  • Place of Presentation: Oslo
  • Year and Date: 2008-07-30
• [Presentation] Uncovering latent a new ligation site for the glycopeptide synthsis (2008)
  • Author(s): Ryo Okamoto
  • Organizer: 24^<th> International Carbohydrate Synpojium
  • Place of Presentation: Oslo
  • Year and Date: 2008-07-30
• [Presentation] Interaction analysis of glycoprobes and lectin with ^<19>F NMR (2008)
  • Author(s): Yoichi Takeda
  • Organizer: 24th Interhational Carbohydrate Symposium
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2008-07-29
• [Presentation] Analysis of ER glycoprotein quality control system of Aspergillus oryzae (2008)
  • Author(s): Taisuke Watanabe
  • Organizer: 24th International Carbohydrate Symposium
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2008-07-29
• [Presentation] NAP ether-mediated Stereoselective Construction of 1, 2-cis-Rhamnopyranosideo (2008)
  • Author(s): Yong Joo Lee
  • Organizer: 24th International Carbohydrate Symposium
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2008-07-29
• [Presentation] Fluorescently labeled inhibitor for profiling cytoplasmic peptide:/N-glycanaseo (2008)
  • Author(s): Ichiro Matsuo
  • Organizer: 24th International Carbohydrate Symposium
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2008-07-29
• [Presentation] Effects of macromolecular crowding on glycoprotein processing enzymes (2008)
  • Author(s): Kiichiro Totani
  • Organizer: 24th International Carbohydrate Symposium
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2008-07-29
• [Presentation] Glycobiology-oriented synthesis (2008)
  • Author(s): Yukishige Ito
  • Organizer: 24th International Carbohydrate Symposium
  • Place of Presentation: Oslo, Norway
  • Year and Date: 2008-07-28
• [Presentation] 化学合成を基盤とする糖鎖生物学へのアプローチ (2008)
  • Author(s): 伊藤幸成
  • Organizer: 理研-東工大連携シンポジウム「新たな分子化学創発を目指して」
  • Place of Presentation: 和光
  • Year and Date: 2008-06-17
• [Presentation] 有機合成化学で糖タンパク質糖鎖の機能をしらべる (2008)
  • Author(s): 伊藤幸成
  • Organizer: 第9回関西グライコサイエンスフォーラム
  • Place of Presentation: 大阪
  • Year and Date: 2008-05-17
• [Presentation] Efficient construction of β-L-rhamnopyranosyl linkage found in bacterial polysaccharides (2008)
  • Author(s): 李龍柱
  • Organizer: 第55回有機合成化学協会関東支部シンポジウム
  • Place of Presentation: 野田
  • Year and Date: 2008-05-09
• [Presentation] 結核菌細胞壁成分アラビナンの合成研究 (2008)
  • Author(s): 石渡明弘
  • Organizer: 第55回有機合成化学協会関東支部シンポジウム
  • Place of Presentation: 野田
  • Year and Date: 2008-05-09
• [Presentation] 有機合成化学で糖タンパク質の動態を観る (2008)
  • Author(s): 伊藤幸成
  • Organizer: 日本化学会第88春季年会
  • Place of Presentation: 名古屋
  • Year and Date: 2008-03-29
• [Presentation] 糖鎖生物学の推進をめざす有機合成化学 (2008)
  • Author(s): 伊藤幸成
  • Organizer: SORSTジョイントシンポジウム「有機合成力」-そのダイナミズム
  • Place of Presentation: 東京
  • Year and Date: 2008-01-30
• [Presentation] ヒト複合型糖鎖をもつ糖タンパク質の化学合成 (2008)
  • Author(s): 梶原 康宏
  • Organizer: 理研シンポジウム
  • Place of Presentation: 和光
  • Year and Date: 2008-01-25
• [Presentation] Synergistic solvent effect in 1, 2-cis-glycoside formation (2008)
  • Author(s): Akihiro Ishiwata
  • Organizer: 235th American chemical Society National Meeting and Exposition
  • Place of Presentation: New Orleans, USA
• [Presentation] ベンジル保護型β-ガラクトシドの収斂的合成法の開発と複雑な糖鎖合成への展開 (2008)
  • Author(s): 植木章晴
  • Organizer: 第28回日本糖質学会年会
  • Place of Presentation: つくば
• [Presentation] 選択的修飾を可能とするLacNAc含有糖タンパク質糖鎖の合成研究 (2008)
  • Author(s): 川平恵太
  • Organizer: 第28回日本糖質学会年会
  • Place of Presentation: つくば
• [Presentation] Azide as a protecting group for lysine side chains on the solid phase peptide synthesis oriented toward the petide condensation by the thioester method (2008)
  • Author(s): Hidekazu Katayama
  • Organizer: 30^<th> European peptide symposium
  • Place of Presentation: Helsinki
• [Presentation] The application of N-alkylcysteine (NAC)-assisted thioesterification to the synthesis of polypeptides by the thioester method (2008)
  • Author(s): Hironobu Hojo
  • Organizer: 30^<th> European peptide symposium
  • Place of Presentation: Helsinki
• [Presentation] Synthetic studies on human glycodelins (2008)
  • Author(s): Takaomi Takenouchi
  • Organizer: 45^<th> JPS
  • Place of Presentation: 東京
• [Presentation] Development of a novel amino protecting group efficient for the peptide condensation reaction by the thioester method (2008)
  • Author(s): Hidekazu Katayama
  • Organizer: 45^<th> IPS
  • Place of Presentation: 東京
• [Presentation] Study on a novel ligation method at Xaa-Ser/Thr site (2008)
  • Author(s): Chinatsu Ozawa
  • Organizer: 45^<th> JPS
  • Place of Presentation: 東京
• [Presentation] バイオ高分子としての糖タンパク質を有機合成化学の視点で観る (2007)
  • Author(s): 伊藤幸成
  • Organizer: 第56回高分子討論会
  • Place of Presentation: 名古屋
  • Year and Date: 2007-09-20
• [Presentation] Synthetic approaches to glycoprotein functions (2007)
  • Author(s): Yukishige Ito
  • Organizer: The 234th American Chemical Society National Meeting
  • Place of Presentation: Boston, USA
  • Year and Date: 2007-08-22
• [Presentation] Glycoprotein functions: Chemist's point of view (2007)
  • Author(s): Yukishige Ito
  • Organizer: RIKEN International Workshop, US-Japan Workshop on Chemical Biology
  • Place of Presentation: Wako, Japan
  • Year and Date: 2007-06-05
• [Presentation] A novel method for the preparation of peptide thioester and its application to glycoprotein synthesis. (2007)
  • Author(s): Hironobu Hojo
  • Organizer: 20th American Peptide Symposium
  • Place of Presentation: Montreal
• [Presentation] Chemical synthesis of glycoproteins having intact and homogeneouscomplex type oligosaccharides (2007)
  • Author(s): Yasuhiro Kajihara
  • Organizer: Gordon Reserch Conferences Corbohydrates
  • Place of Presentation: Tilton, NH.
• [Presentation] ベンジル保護型β-ガラクトシドの収歛的合成 (2007)
  • Author(s): 植木章晴
  • Organizer: 第27回日本糖質学会年会
  • Place of Presentation: 福岡
• [Presentation] N-結合型糖アミノ酸の合成 (2007)
  • Author(s): 小松恵子
  • Organizer: 第27回日本糖質学会年会
  • Place of Presentation: 福岡
• [Presentation] 新規なチオエステル法を利用した糖ペプチドデンドリマーの合成 (2007)
  • Author(s): 小澤千那津
  • Organizer: 第27回日本糖質学会年会
  • Place of Presentation: 福岡
• [Presentation] Synthesis of glycopeptide dendrimer by the thioester method (2007)
  • Author(s): Hironobu Hojo
  • Organizer: 4th International Peptide Symposium
  • Place of Presentation: Cairns
• [Presentation] Synthesis of MUC1 glycopeptide dendrimer (2007)
  • Author(s): 小澤千那津
  • Organizer: 44th Japanese Peptide Symposium
  • Place of Presentation: 富山
• [Presentation] Chemical synthesis of androgenic gland hormone of the terrestrial isopod, Armadillidium vulgare. (2007)
  • Author(s): 片山秀和
  • Organizer: 44th Japanese Peptide Symposium
  • Place of Presentation: 富山
• [Presentation] Chemical Synthesis of Glycoprotein Having Human Complex Type Oligosaccharides. (2007)
  • Author(s): Yasuhiro Kajihara
  • Organizer: Chemical Protein Synthesis Meeting
  • Place of Presentation: Heron Island, Australia
• [Presentation] Chemical synthesis of glycoproteins having human complex type oligosaccharide (2007)
  • Author(s): Yasuhiro Kajihara
  • Organizer: 4th International Peptide Synposium
  • Place of Presentation: Ciarns, Australia
• [Book] 糖ペプチド, 糖タンパク質の化学合成法 バイオテクノロジーシリ・ズ「複合糖質の化学と最新応用技術」 (2010)
  • Author(s): 北條裕信
  • Total Pages: 8
  • Publisher: シーエムシー出版
• [Book] 糖鎖科学の新展開 -機能解明・次世代型材料・医薬品開発に向けて- (2005)
  • Author(s): 伊藤幸成, 松尾一郎, 戸谷希一郎
  • Total Pages: 8
  • Publisher: (株)エヌ・ティー・エス
• [Book] 糖鎖科学の新展開 -機能解明・次世代型材料・医薬品開発に向けて- (2005)
  • Author(s): 眞鍋史乃, 伊藤幸成
  • Total Pages: 7
  • Publisher: (株)エヌ・ティー・エス
• [Book] 糖鎖科学の新展開 -機能解明・次世代型材料・医薬品開発に向けて- (2005)
  • Author(s): 中原義昭, 北條裕信
  • Total Pages: 6
  • Publisher: (株)エヌ・ティー・エス
• [Book] 糖鎖科学の新展開 -機能解明・次世代型材料・医薬品開発に向けて- (2005)
  • Author(s): 梶原康宏
  • Total Pages: 8
  • Publisher: (株)エヌ・ティー・エス
• [Book] 未来を拓く糖鎖科学 (2005)
  • Author(s): 眞鍋史乃, 伊藤幸成
  • Total Pages: 4
  • Publisher: 金芳堂
• [Book] 未来を拓く糖鎖科学 (2005)
  • Author(s): 中原義昭, 北條裕信
  • Total Pages: 2
  • Publisher: 金芳堂
• [Book] 未来を拓く糖鎖科学 (2005)
  • Author(s): 梶原康宏
  • Total Pages: 2
  • Publisher: 金芳堂
• [Remarks] 2009年7月第24回国際糖質学会で国際賞であるThe Roy L.Whistler Intemational Award in Carbohydrate Chemistryを伊藤幸成が受賞した.
• [Remarks] Angewndte.Chem.Int.Ed., 2009, 49, 9557-9560に掲載されたエリスロポイエチンの合成に関する論文(論文8)はin side coverに採用された.その成果は、平成21年10月12日の日本経済新聞の科学面で紹介された.
• [Remarks] J.Am.Chem.Soc., 2008, 130, 501-510に掲載された糖タンパク質の化学合成(論文12)は、12月8日の日本経済新聞社会面、12月12日朝日新聞科学面で、また12月8日のNHK「おはよう日本」で紹介された.
• [Remarks] Eur.J.Org.Chem., 2008, 25, 4250-4263に掲載された高選択的グリコシル化反応に関す論文(論文7)は表紙に採用された.
• [Remarks] J.Biol.Chem., 2006, 281, 31502-31508に掲載されたGlucosidase IIの特異性解析(論文23)は表紙に採用された.
• [Remarks] Tetrahedron Lett., 2005, 46, 4197-4200に掲載された14糖の合成研究(論文28)は表紙に採用された.
• [Remarks] Angewndte.Chem.Int.Ed.2009, 49, 9557-9560. に掲載されたエリスロポエチン誘導体の合成に関する成果が、平成21年10月12日の日本経済新聞の科学面で紹介された
• [Remarks]
  • URL: http://www.el.u-tokai.ac.jp/nakahara/index.html
• [Remarks]
  • URL: http://www.tsc.u-tokai.ac.jp/pubhome/glyco/intro/intro03.html
• [Patent(Industrial Property Rights)] 糖鎖付加AILIM細胞外ドメイン及びその製造方法 (2009)
  • Inventor(s): 梶原康宏, 辻孝, 他
  • Industrial Property Number: 2009-016809
  • Filing Date: 2009-07-16
• [Patent(Industrial Property Rights)] ペプチドの製造方法 (2009)
  • Inventor(s): 北條裕信, 中原義昭
  • Filing Date: 2009-06-10
• [Patent(Industrial Property Rights)] ペプチドの製造方法 (2009)
  • Inventor(s): 北條裕信, 中原義昭
  • Industrial Property Rights Holder: 学校法人東海大学
  • Filing Date: 2009-06-10
  • Overseas
• [Patent(Industrial Property Rights)] 均一な糖鎖構造を有するエリスロポエチン誘導体 (2008)
  • Inventor(s): 梶原康宏, デレク・マクミラン, 平野桐子, 他
  • Filing Date: 2008-08-18
• [Patent(Industrial Property Rights)] ペプチドの製造方法 (2008)
  • Inventor(s): 北條裕信, 中原義昭
  • Industrial Property Rights Holder: 学校法人東海大学
  • Filing Date: 2008-10-07
• [Patent(Industrial Property Rights)] 均一な糖鎖構造を有するエリスロポエチン誘導体 (2008)
  • Inventor(s): 梶原康宏, 平野桐子, 深江一博
  • Industrial Property Rights Holder: 横浜市立大学、University College London, 大塚化学(株)
  • Industrial Property Number: 2008-209583
  • Filing Date: 2008-08-18
• [Patent(Industrial Property Rights)] 糖タンパク質の製造方法及びスクリーニング方法 (2008)
  • Inventor(s): 梶原康宏, 深江一博
  • Industrial Property Rights Holder: 横浜市立大学、大塚化学(株)
  • Industrial Property Number: 2008-211144
  • Filing Date: 2008-08-19
• [Patent(Industrial Property Rights)] 糖鎖ライブラリの作成方法及びその利用 (2007)
  • Inventor(s): 松尾一郎, 伊藤幸成
  • Filing Date: 2007-07-23
• [Patent(Industrial Property Rights)] ペプチドのチオエステル化合物の製造方法 (2007)
  • Inventor(s): 梶原康宏, 山本直樹, 他
  • Filing Date: 2007-03-28
• [Patent(Industrial Property Rights)] セリンを有するペプチドの製造方法 (2007)
  • Inventor(s): 梶原康宏以下4名
  • Industrial Property Rights Holder: 横浜市立大学、大塚化学(株)
  • Filing Date: 2007-07-31
• [Patent(Industrial Property Rights)] ペプチドチオエステルの製造方法 (2006)
  • Inventor(s): 北條裕信, 中原義昭
  • Industrial Property Number: 2006-275022
  • Filing Date: 2006-10-06
• [Patent(Industrial Property Rights)] コア4型構造を有するO-結合型糖タンパク質関連化合物およびその製造方法 (2006)
  • Inventor(s): 中原義昭, 北條裕信, 植木章晴
  • Industrial Property Number: 2006-200069
  • Filing Date: 2006-07-21
• [Patent(Industrial Property Rights)] 糖鎖ライブラリの作成方法及びその利用 (2006)
  • Inventor(s): 松尾一郎, 伊藤幸成
  • Filing Date: 2006-04-27
• [Patent(Industrial Property Rights)] コア4型構造を有する0-結合型糖タンパク質関連化合物およびその製造方法 (2006)
  • Inventor(s): 中原義昭, 北條裕信, 植木章晴
  • Industrial Property Rights Holder: 学校法人東海大学
  • Industrial Property Number: 2006-200069
  • Filing Date: 2006-07-21