Comprehensive evaluation of residual pharmaceuticals in the aquatic environment and their removal efficiency in wastewater treatment plant

• Project/Area Number: 17H00786
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Risk sciences of radiation and chemicals
• Research Institution: Kyoto University
• Principal Investigator: Nakada Norihide 京都大学, 工学研究科, 講師 (00391615)
• Co-Investigator(Kenkyū-buntansha): 井原 賢 京都大学, 工学研究科, 特定助教 (70450202)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥42,380,000 (Direct Cost: ¥32,600,000、Indirect Cost: ¥9,780,000); Fiscal Year 2019: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000); Fiscal Year 2018: ¥13,910,000 (Direct Cost: ¥10,700,000、Indirect Cost: ¥3,210,000); Fiscal Year 2017: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
• Keywords: 薬理活性 / 環境医薬品 / 予測スクリーニング / バイオアッセイ / 精密質量分析 / 下水処理 / ノンターゲット分析 / 生理活性 / 医薬品汚染 / 下水処理性能評価 / 下水処理性能 / ターゲット分析 / 医薬品による環境汚染 / 四重極飛行時間型質量分析計

Outline of Final Research Achievements

The purpose of this study is to gain a further understanding of residual pharmaceuticals in the aquatic environment and to assess their impact on the aquatic organisms in the future. Targeted instrumental analysis and a bioassay that can detect physiological activity directly from water samples were applied to wastewater samples together with suspect screening based on the approaches to qualify untargeted or unknown active substances. A total of 36 types of G-protein-conjugated receptor antagonists, including 4 metabolites or degradation products were quantified in the wastewater samples from 5 wastewater treatment plants (WWTPs). The results revealed that low removal efficiencies of them and their activities by the conventional wastewater treatment and the occurrence of metabolites or degradation products at a relatively high concentrations and frequency of detection in the effluent of WWTPs.

Academic Significance and Societal Importance of the Research Achievements

水環境中に残留する医薬品の定量は、これまでは分析用標準物質が販売されている比較的古い薬剤に対するターゲット分析が主流であったが、それでは残留医薬品の評価に漏れが生じている可能性がある。また、今後の残留医薬品の評価においては、化学物質としてではなく、薬効成分として評価する必要がある。このような背景から、水試料からの薬理活性の直接の検出と予測スクリーニングを組合せ、残留医薬品を評価した点は革新的であると言える。

Research Products

• [Int'l Joint Research] Centre for Ecology and Hydrology/Brunel University London(英国)
• [Int'l Joint Research] National Environment Monitoring Centre(中国)
• [Journal Article] N-nitrosodimethylamine formation potential (NDMA-FP) of ranitidine remains after chlorination and/or photo-irradiation: Identification of transformation products in combination with NDMA-FP test (2021)
  • Author(s): Zhao Bo、Zhou Jiajun、Nakada Norihide
  • Journal Title: Chemosphere Volume: 267 Pages: 129200-129200
  • DOI: 10.1016/j.chemosphere.2020.129200
  • Peer Reviewed
• [Journal Article] Biological Activity-Based Prioritization of Pharmaceuticals in Wastewater for Environmental Monitoring: G Protein-Coupled Receptor Inhibitors (2020)
  • Author(s): Zhang Han、Ihara Mariko O.、Nakada Norihide、Tanaka Hiroaki、Ihara Masaru
  • Journal Title: Environmental Science & Technology Volume: 54 Issue: 3 Pages: 1720-1729
  • DOI: 10.1021/acs.est.9b05768
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Learning from the past and considering the future of chemicals in the environment (2020)
  • Author(s): Johnson Andrew C.、Jin Xiaowei、Nakada Norihide、Sumpter John P.
  • Journal Title: Science Volume: 367 Issue: 6476 Pages: 384-387
  • DOI: 10.1126/science.aay6637
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] 水環境中の医薬品の存在状況 (2020)
  • Author(s): 中田典秀
  • Journal Title: 化学物質と環境 Volume: 161 Pages: 4-6
• [Presentation] Biological activity-based prioritization of pharmaceuticals in wastewater of England and Japan (2019)
  • Author(s): Han Zhang, Norihide Nakada, Masaru Ihara, Hiroaki Tanaka
  • Organizer: The 5th Asian Symposium on Water Reuse -Technology Renovation and Risk Management
  • Int'l Joint Research
• [Presentation] NDMA Formation from Ranitidine Affected by Chlorination and/or Photo-irradiation (2019)
  • Author(s): Jiajun Zhou, Bo Zhao, Hiroaki Tanaka, Norihide Nakada
  • Organizer: 11th Micropol & Ecohazard Conference
  • Int'l Joint Research
• [Presentation] Screening of Physiological Activity in Wastewater Effluents by Combination of in vitro Bioassay, Target Analysis and Suspect Screening (2019)
  • Author(s): Norihide Nakada, Han Zhang, Masaru Ihara
  • Organizer: 11th Micropol & Ecohazard Conference
  • Int'l Joint Research
• [Presentation] PPCPsの環境中での存在状況 (2019)
  • Author(s): 中田典秀
  • Organizer: 第33回環境ホルモン学会講演会
  • Invited
• [Presentation] PPCPｓの水生生物への影響：神経系薬を中心に (2019)
  • Author(s): 井原賢
  • Organizer: 第33回環境ホルモン学会講演会
  • Invited
• [Presentation] バイオアッセイ、ターゲット分析、予測スクリーニングによる下水処理水中の各種受容体拮抗作用物質の探索 (2019)
  • Author(s): 中田典秀、張晗、井原賢
  • Organizer: 日本水環境学会 第53回日本水環境学会年会
• [Presentation] 生理活性に基づく下水処理水中のアンジオテンシンII 受容体拮抗作用（薬）のターゲット分析および予測スクリーニング (2018)
  • Author(s): 中田典秀、張晗、井原賢
  • Organizer: 日本環境化学会 第27回環境化学討論会
• [Presentation] アンジオテンシンII受容体拮抗薬の都市水環境における存在実態 (2018)
  • Author(s): 中田典秀、長谷川英資、井原賢
  • Organizer: 第52回に本水環境学会年会
• [Presentation] 生理活性に基づく下水処理水中のアンジオテンシンII 受容体拮抗作用（薬）のターゲット分析および予測スクリーニング (2018)
  • Author(s): 中田典秀、張晗、井原賢
  • Organizer: 第27回環境化学討論会