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Elucidation of mechanisms and expansion of application range of sonoporation by in situ observation of bubble-cell interaction

Research Project

Project/Area Number 17H00864
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Medical systems
Research InstitutionHokkaido University

Principal Investigator

Kudo Nobuki  北海道大学, 情報科学研究院, 准教授 (30271638)

Co-Investigator(Kenkyū-buntansha) 松崎 典弥  大阪大学, 工学研究科, 教授 (00419467)
鈴木 亮  帝京大学, 薬学部, 教授 (90384784)
佐々木 東  北海道大学, 獣医学研究院, 助教 (00754532)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000)
Fiscal Year 2019: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥33,150,000 (Direct Cost: ¥25,500,000、Indirect Cost: ¥7,650,000)
Keywords超音波治療 / ソノポレーション / 血液脳関門開放 / 薬物送達 / マイクロバブル / 高速度観察 / 血管内皮細胞 / 3次元細胞培養 / 微小気泡 / 高速度顕微観察 / 3次元細胞培養 / 毛細血管 / 樹状細胞 / 側方観察 / ファインバブル / 細胞培養
Outline of Final Research Achievements

Sonoporation and blood-brain (tumor) barrier (BBB/BTB) opening techniques achieve local drug delivery by ultrasound irradiation with microbubbles. The former delivers drugs into cells by increasing the cell membranes permeability, and the latter facilitates drug transfer from blood to perivascular tissues by loosening vascular endothelial cell junctions. In this study, we created three models that simulate the in vivo situation in which three types of cells exist: superficial cells on the soft tissue, vascular endothelial cells that manage drug transfer from the blood, and dendritic cells that are responsible for immunity. The cell-bubble interaction in the model was observed in situ using high-speed confocal microscope to elucidate the mechanisms of sonoporation and blood barrier opening and their dependence on ultrasound irradiation conditions.

Academic Significance and Societal Importance of the Research Achievements

ソノポレーションや血液脳(腫瘍)関門の開放の実現に向け,実験動物を用いる検討は盛んに行われているが,ごく少数を除き臨床応用には至っていない.その原因は,超音波周波数で起きる細胞レベルの変化の観察が工学的に困難であり,現象の基礎的理解が不十分なまま応用研究が先行したことにある.本研究では,現象の解明鳥飼いを目指して世界最高性能を有する観察システムを開発するとともに,生体内を模擬するモデルを開発し,生体内で実際に起きている現象の機序を解明した.本研究成果を端緒に現象の理解が進めばソノポレーション・薬物送達・免疫治療の促進など,超音波を用いた低侵襲治療法の実用性が大幅な進歩が見込まれる.

Report

(4 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (100 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (4 results) Journal Article (32 results) (of which Int'l Joint Research: 11 results,  Peer Reviewed: 28 results,  Open Access: 6 results) Presentation (49 results) (of which Int'l Joint Research: 28 results,  Invited: 30 results) Book (6 results) Patent(Industrial Property Rights) (9 results) (of which Overseas: 3 results)

  • [Int'l Joint Research] University of the Witwatersrand(南アフリカ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] University of Tours(フランス)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Eindhoven University of Technology(オランダ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Moscow State University(ロシア連邦)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] High-speed footage shows transient ultrasonic nucleation of different hydrophobic particles in suspension2020

    • Author(s)
      Postema Michiel、Matsumoto Ryonosuke、Shimizu Ri-ichiro、Poortinga Albert T.、Kudo Nobuki
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 59 Issue: SK Pages: SKKD07-SKKD07

    • DOI

      10.35848/1347-4065/ab7f19

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Asymmetric oscillations of endoskeletal antibubbles2020

    • Author(s)
      Kudo Nobuki、Uzbekov Rustem、Matsumoto Ryunosuke、Shimizu Ri-ichiro、Carlson Craig S.、Anderton Nicole、Deroubaix Aurelie、Penny Clement、Poortinga Albert T.、Rubin David M.、Bouakaz Ayache、Postema Michiel
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 59 Issue: SK Pages: SKKE02-SKKE02

    • DOI

      10.35848/1347-4065/ab79e7

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Visualization of wall propagation and surface reflection effects on ultrasound fields generated inside a small container2019

    • Author(s)
      Aikawa Takeshi、Kudo Nobuki
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 58 Issue: SG Pages: SGGE11-SGGE11

    • DOI

      10.7567/1347-4065/ab1644

    • NAID

      210000156396

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Low-intensity pulsed ultrasound modifies effects of a myelin-related growth inhibitor and enhances neurite re-growth2019

    • Author(s)
      Sasaki Noboru、Kudo Nobuki、Ohta Hiroshi、Takiguchi Mitsuyoshi
    • Journal Title

      IEEE International Ultrasonics Symposium Proceedings

      Volume: - Pages: 1-4

    • DOI

      10.1109/ultsym.2019.8926300

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Culture Conditions on Sonoporation of Dendritic Cells2019

    • Author(s)
      Matsumoto Ryunosuke、Kudo Nobuki
    • Journal Title

      IEEE International Ultrasonics Symposium Proceedings

      Volume: - Pages: 1-4

    • DOI

      10.1109/ultsym.2019.8925637

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Liquefied Microcapsules as Dual‐Microcarriers for 3D+3D Bottom‐Up Tissue Engineering2019

    • Author(s)
      Correia Clara R.、Bjorge Isabel M.、Zeng Jinfeng、Matsusaki Michiya、Mano Joao F.
    • Journal Title

      Advanced Healthcare Materials

      Volume: 8 Issue: 22 Pages: 1901221-1901221

    • DOI

      10.1002/adhm.201901221

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Porphyromonas gingivalis induces penetration of lipopolysaccharide and peptidoglycan through the gingival epithelium via degradation of junctional adhesion molecule 12019

    • Author(s)
      Takeuchi Hiroki、Sasaki Naoko、Yamaga Shunsuke、Kuboniwa Masae、Matsusaki Michiya、Amano Atsuo
    • Journal Title

      PLOS Pathogens

      Volume: 15 Issue: 11 Pages: e1008124-e1008124

    • DOI

      10.1371/journal.ppat.1008124

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Inhibitory effect of carbonyl reductase 1 against peritoneal progression of ovarian cancer: evaluation by ex vivo 3D-human peritoneal model2019

    • Author(s)
      Oikiri Hiroe、Asano Yoshiya、Matsusaki Michiya、Akashi Mitsuru、Shimoda Hiroshi、Yokoyama Yoshihito
    • Journal Title

      Molecular Biology Reports

      Volume: 46 Issue: 5 Pages: 4685-4697

    • DOI

      10.1007/s11033-019-04788-6

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Extracellular Matrix Microfiber Papers for Constructing Multilayered 3D Composite Tissues2019

    • Author(s)
      Nakatsuji Hirotaka、Matsusaki Michiya
    • Journal Title

      ACS Biomaterials Science & Engineering

      Volume: 5 Issue: 11 Pages: 5610-5614

    • DOI

      10.1021/acsbiomaterials.9b00090

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Fabrication of Perfusable Pseudo Blood Vessels by Controlling Sol-Gel Transition of Gellan Gum Templates2019

    • Author(s)
      Matsusaki Michiya、Ikeguchi Haruki、Kubo Chihiro、Sato Hisako、Kuramochi Yuzuru、Takagi Daisuke
    • Journal Title

      ACS Biomaterials Science & Engineering

      Volume: in press Issue: 11 Pages: 5637-5643

    • DOI

      10.1021/acsbiomaterials.8b01272

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 3D collagen microfibers stimulate the functionality of preadipocytes and maintain the phenotype of mature adipocytes for long term cultures2019

    • Author(s)
      Louis Fiona、Kitano Shiro、Mano Joao F.、Matsusaki Michiya
    • Journal Title

      Acta Biomaterialia

      Volume: 84 Pages: 194-207

    • DOI

      10.1016/j.actbio.2018.11.048

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Lipid nanoparticles of Type-A CpG D35 suppress tumor growth by changing tumor immune-microenvironment and activate CD8 T cells in mice2019

    • Author(s)
      Munakata Lisa、Tanimoto Yoshihiko、Osa Akio、Meng Jie、Haseda Yasunari、Naito Yujiro、Machiyama Hirotomo、Kumanogoh Atsushi、Omata Daiki、Maruyama Kazuo、Yoshioka Yasuo、Okada Yoshiaki、Koyama Shohei、Suzuki Ryo、Aoshi Taiki
    • Journal Title

      Journal of Controlled Release

      Volume: 313 Pages: 106

    • DOI

      10.1016/j.jconrel.2019.09.011

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of encapsulated gas on stability of lipid-based microbubbles and ultrasound-triggered drug delivery2019

    • Author(s)
      Omata Daiki、Maruyama Tamotsu、Unga Johan、Hagiwara Fumiko、Munakata Lisa、Kageyama Saori、Shima Tadamitsu、Suzuki Yuno、Maruyama Kazuo、Suzuki Ryo
    • Journal Title

      Journal of Controlled Release

      Volume: 311-312 Pages: 65

    • DOI

      10.1016/j.jconrel.2019.08.023

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development and evaluation of stability and ultrasound response of DSPC-DPSG-based freeze-dried microbubbles2019

    • Author(s)
      Unga Johan、Omata Daiki、Kudo Nobuki、Ueno Soki、Munakata Lisa、Shima Tadamitsu、Suzuki Ryo、Maruyama Kazuo
    • Journal Title

      Journal of Liposome Research

      Volume: 29 Issue: 4 Pages: 368

    • DOI

      10.1080/08982104.2018.1556294

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vitro placenta barrier model using primary human trophoblasts, underlying connective tissue and vascular endothelium2019

    • Author(s)
      Nishiguchi Akihiro、Gilmore Catherine、Sood Aman、Matsusaki Michiya、Collett Gavin、Tannetta Dionne、Sargent Ian L.、McGarvey Jennifer、Halemani Nagaraj D.、Hanley Jon、Day Fiona、Grant Simon、Murdoch-Davis Catherine、Kemp Helena、Verkade Paul、Aplin John D.、Akashi Mitsuru、Case C Patrick
    • Journal Title

      Biomaterials

      Volume: 192 Pages: 140-148

    • DOI

      10.1016/j.biomaterials.2018.08.025

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Study on dependence of mechanotransduction sensitivity on cytoskeletal development2018

    • Author(s)
      Shota Negishi, Nobuki. Kudo
    • Journal Title

      IEEE International Ultrasonics Symposium Proceedings

      Volume: - Pages: 1-4

    • DOI

      10.1109/ultsym.2018.8579924

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of a microvascular phantom for studies on microbubble dynamics and bubble-cell interaction inside a capillary2018

    • Author(s)
      Shinij Imai, Nobuki Kudo
    • Journal Title

      IEEE International Ultrasonics Symposium Proceedings

      Volume: - Pages: 1-4

    • DOI

      10.1109/ultsym.2018.8579713

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] フォーカストシャドウグラフ法を用いた小型簡易な超音波音場可視化装置の実用的設計2018

    • Author(s)
      門田智明,工藤信樹
    • Journal Title

      電子情報通信学会技術研究報告

      Volume: 118(106) Pages: 1-6

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 高音圧短パルスと低音圧長パルスの超音波照射下におけるソノポレーション機序に関する検討2018

    • Author(s)
      工藤信樹, 今井慎司, 磯野朱音
    • Journal Title

      日本超音波医学会基礎技術研究会資料

      Volume: 2018(2) Pages: 63-66

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Bioinspired multilayer membranes as potential adhesive patches for skin wound healing2018

    • Author(s)
      Sousa Maria P.、Neto Ana I.、Correia Tiago R.、Miguel Sonia P.、Matsusaki Michiya、Correia Ilidio J.、Mano Joao F.
    • Journal Title

      Biomaterials Science

      Volume: 6 Issue: 7 Pages: 1962-1975

    • DOI

      10.1039/c8bm00319j

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] In vitro 3D blood/lymph-vascularized human stromal tissues for preclinical assays of cancer metastasis2018

    • Author(s)
      Nishiguchi Akihiro、Matsusaki Michiya、Kano Mitsunobu R.、Nishihara Hiroshi、Okano Daisuke、Asano Yoshiya、Shimoda Hiroshi、Kishimoto Satoko、Iwai Soichi、Akashi Mitsuru
    • Journal Title

      Biomaterials

      Volume: 179 Pages: 144-155

    • DOI

      10.1016/j.biomaterials.2018.06.019

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Evaluation of frequency-dependent ultrasound attenuation in transparent medium using focused shadowgraph technique2017

    • Author(s)
      Iijima Yukina、Kudo Nobuki
    • Journal Title

      Japanese Journal of Applied Physics

      Volume: 56 Issue: 7S1 Pages: 07JF13-07JF13

    • DOI

      10.7567/jjap.56.07jf13

    • NAID

      210000148075

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spatial and temporal profile of cisplatin delivery by ultrasound-assisted intravesical chemotherapy in a bladder cancer model2017

    • Author(s)
      Sasaki Noboru、Ishi Kazuhiro、Kudo Nobuki、Nakayama Shouta M. M.、Nakamura Kensuke、Morishita Keitaro、Ohta Hiroshi、Ishizuka Mayumi、Takiguchi Mitsuyoshi
    • Journal Title

      PLOS ONE

      Volume: 12 Issue: 11 Pages: e0188093-e0188093

    • DOI

      10.1371/journal.pone.0188093

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A high-speed microscopic system for observation of bubble-cell interaction from a lateral direction2017

    • Author(s)
      Isono Akane、Kudo Nobuki
    • Journal Title

      Proceedings of IEEE International Ultrasonics Symposium

      Volume: 2017 Pages: 1-4

    • DOI

      10.1109/ultsym.2017.8092145

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] In vivoソノポレーションに向けた微小気泡―細胞間相互作用解明のための顕微観察手法の開発2017

    • Author(s)
      磯野朱音,工藤信樹
    • Journal Title

      日本超音波医学会 基礎技術研究会資料

      Volume: 2017 Pages: 45-50

    • Related Report
      2017 Annual Research Report
  • [Journal Article] In vitroソノポレーションにおける電界誘起気泡の有用性に関する基礎的研究2017

    • Author(s)
      常世 晶,工藤 信樹
    • Journal Title

      電子情報通信学会技術研究報告

      Volume: 117 Pages: 1-6

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Transplantation of artificial human lymphatic vascular tissues fabricated using a cell-accumulation technique and their engraftment in mouse tissue with vascular remodelling2017

    • Author(s)
      Asano Yoshiya、Shimoda Hiroshi、Matsusaki Michiya、Akashi Mitsuru
    • Journal Title

      Journal of Tissue Engineering and Regenerative Medicine

      Volume: 12 Issue: 3

    • DOI

      10.1002/term.2570

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Construction of artificial human peritoneal tissue by cell-accumulation technique and its application for visualizing morphological dynamics of cancer peritoneal metastasis2017

    • Author(s)
      Asano Yoshiya、Odagiri Tadashi、Oikiri Hiroe、Matsusaki Michiya、Akashi Mitsuru、Shimoda Hiroshi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 494 Issue: 1-2 Pages: 213-219

    • DOI

      10.1016/j.bbrc.2017.10.050

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Three-dimensional cultured tissue constructs that imitate human living tissue organization for analysis of tumor cell invasion2017

    • Author(s)
      Iwai Soichi、Kishimoto Satoko、Amano Yuto、Nishiguchi Akihiro、Matsusaki Michiya、Takeshita Akinori、Akashi Mitsuru
    • Journal Title

      Journal of Biomedical Materials Research Part A

      Volume: 106A Issue: 2 Pages: 292-300

    • DOI

      10.1002/jbm.a.36319

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Epidermal growth factor receptor-targeted sonoporation with microbubbles enhances therapeutic efficacy in a squamous cell carcinoma model2017

    • Author(s)
      Hirabayashi F, Iwanaga K, Okinaga T, Takahashi O, Ariyoshi W, Suzuki R, Sugii M, Maruyama K, Tominaga K, Nishihara T
    • Journal Title

      PLoS One

      Volume: 12 Issue: 9 Pages: e0185293-e0185293

    • DOI

      10.1371/journal.pone.0185293

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Evaluation of pharmacokinetic and pharmacodynamic profiles of liposomes for the cell type-specific delivery of small molecule drugs2017

    • Author(s)
      Dasa Siva Sai Krishna、Suzuki Ryo、Mugler Emily、Chen Lanlin、Jansson-Lofmark Rasmus、Michaelsson Erik、Lindfors Lennart、Klibanov Alexander L.、French Brent A.、Kelly Kimberly A.
    • Journal Title

      Nanomedicine

      Volume: 13 Issue: 8 Pages: 2565

    • DOI

      10.1016/j.nano.2017.07.005

    • URL

      https://localhost/en/publications/7044d768-2a9c-4b70-a67b-6bac53c989b5

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The development of mechanically formed stable nanobubbles intended for sonoporation-mediated gene transfection2017

    • Author(s)
      Abdalkader R, Kawakami S, Unga J, Higuchi Y, Suzuki R, Maruyama K, Yamashita F, Hashida M
    • Journal Title

      Drug Delivery

      Volume: 24 Issue: 1 Pages: 320

    • DOI

      10.1080/10717544.2016.1250139

    • NAID

      120006987449

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] Cancer therapy by the combination of ultrasound contrast imaging agent mediated sonotherapy and immunotherapy2020

    • Author(s)
      Suzuki Ryo, Omata Daiki, Maruyama Kazuo
    • Organizer
      25th European Symposium on Ultrasound Contrast Imaging
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Mechanical interactions of oscillating microbubbles with cells and a capillary under ultrasound2019

    • Author(s)
      Kudo Nobuki
    • Organizer
      The 6th International Symposium on Bioimaging
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Roles of microbubbles in sonoporation and BBB opening2019

    • Author(s)
      Kudo Nobuki
    • Organizer
      The 11th Asian Conference on Ultrasound Contrast Imaging
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 樹状細胞による微小気泡の貪食が超音波照射下における気泡径振動に与える影響(最優秀演題賞)2019

    • Author(s)
      松本龍之介, 工藤信樹
    • Organizer
      第18回日本超音波治療研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 超音波照射下における微小気泡と血管壁相互作用の観察を目指した三次元毛細血管モデルの開発(優秀演題賞)2019

    • Author(s)
      清水理一郎, 今井慎二,工藤信樹
    • Organizer
      日本超音波医学会第49回北海道地方会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 樹状細胞の伸展状態がソノポレーションによる気泡のふるまいと膜損傷の発生に与える影響(新人賞)2019

    • Author(s)
      松本龍之介, 工藤信樹
    • Organizer
      日本超音波医学会第92回学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Nano-coating technology on cell surfaces for tissue engineering2019

    • Author(s)
      Matsusaki Michiya
    • Organizer
      Utrecht summer school, Utrecht medical university
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Biomedical functional materials based on poly(gamma-glutamic acid)2019

    • Author(s)
      Matsusaki Michiya
    • Organizer
      The 7th International Conference on Bio-based Polymers (ICBP2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細胞間接着を誘起するバイオマテリアルを用いた3次元培養法の創製と毒性試験への応用2019

    • Author(s)
      松崎典弥
    • Organizer
      CBI学会2019年大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 三次元組織モデルの可能性と課題、今後への期待2019

    • Author(s)
      松崎典弥
    • Organizer
      第19回遺伝子・デリバリー研究会夏期セミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ナノ構造制御高分子材料による細胞界面の制御と組織工学への応用2019

    • Author(s)
      松崎典弥
    • Organizer
      高分子同友会勉強会、高分子学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] バイオプリンターを利用した組織工学による再生医療・創薬研究2019

    • Author(s)
      松崎典弥
    • Organizer
      第37回日本骨代謝学会学術集会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Development of cancer immuno-hyperthermia therapy with ultrasound and microbubbles2019

    • Author(s)
      鈴木亮、小俣大樹、丸山一雄
    • Organizer
      日本ハイパーサーミア学会第36回大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] The Induction of Effective Cancer Immune Response by The Combination of Lipid Based Bubbles and Therapeutic Ultrasound2019

    • Author(s)
      Suzuki Ryo, Omata Daiki, Munakata Lisa, Shima Tadamitsu, Suzuki Yuno, Kageyama Saori, Hagiwara Fumiko, Maruyama Kazuo, Suzuki Ryo
    • Organizer
      American Association of Pharmaceutical Scientists (AAPS) 2019 PHARMSCI 360
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 超音波を利用したイムノモジュレーションによるがん治療戦略と今後の展望2019

    • Author(s)
      鈴木亮、小俣大樹、丸山一雄
    • Organizer
      第18回 日本超音波治療研究会(JSTU2021)
    • Related Report
      2019 Annual Research Report
  • [Presentation] Sonoporation of Cells using Contrast Agent Microbubbles: A Technique for Gene Transduction and Drug Delivery2019

    • Author(s)
      Nobuki Kudo
    • Organizer
      WIFUMB2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 超音波セラノスティクスの実現に向けて2019

    • Author(s)
      鈴木 亮
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 超音波を利用した血管透過性亢進による新規薬物・遺伝子デリバリー法の開発2019

    • Author(s)
      鈴木 亮
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Development of a long-circulating ultrasound contrast agent2019

    • Author(s)
      Ryo Suzuki
    • Organizer
      The 24th European symposium on Ultrasound Contrast Imaging
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sonoporation of Cells using Contrast Agent Microbubbles: A Technique for Gene Transduction and Drug Delivery2018

    • Author(s)
      Nobuki Kudo
    • Organizer
      WIFUMB2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Studies on Mechanisms of Sonoporation under Exposure of _High-pressure Short Pulse and _Low-pressure Long Pulse2018

    • Author(s)
      Nobuki Kudo
    • Organizer
      KSTU2018 (Korean Sciety of Therapeutic Ultrasound)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] High-speed Microscopic Observation System for Studies on Mechanisms of Sonoporation and BBB Opening2018

    • Author(s)
      Nobuki Kudo
    • Organizer
      State of the Art in Asia: Interventional & Therapeutic_Ultrasound 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Visualization of wall propagation and water surface reflection effects on ultrasound fields generated inside a small container2018

    • Author(s)
      Takeshi Aikawa, Nobuki Kudo
    • Organizer
      The 39th Symposium on Ultrasonic Electronics(USE2018)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞骨格の発達状態が培養心筋細胞の機械刺激受容感度に与える影響2018

    • Author(s)
      根岸聖太,工藤信樹
    • Organizer
      第17回日本超音波治療研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 超音波の最大負圧が気泡による血管拡張効果に与える影響2018

    • Author(s)
      今井慎司,工藤信樹
    • Organizer
      第17回日本超音波治療研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 造影と治療における微小気泡のダイナミクスの観察2018

    • Author(s)
      工藤信樹
    • Organizer
      第91回日本超音波医学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] フォーカストシャドウグラフ法を用いた音場可視化による HIFU ビーム形状の評価2018

    • Author(s)
      榊飛翔,工藤信樹
    • Organizer
      第91回日本超音波医学会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Cell-interactive nano/micro biomaterials for biomedical application2018

    • Author(s)
      Michiya Matsusaki
    • Organizer
      ICAPPP2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nano/micro-biomaterials for biofabrication of in vitro human tissue models2018

    • Author(s)
      Michiya Matsusaki
    • Organizer
      CHEM2NATURE
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Nano/micro-biomaterials for in vitro construction of human tissue models2018

    • Author(s)
      Michiya Matsusaki
    • Organizer
      ENGE2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Assembly of nano/micro-biomaterials and cells to construct in vitro 3D-tissues2018

    • Author(s)
      Michiya Matsusaki
    • Organizer
      TJK2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Effective tumor growth suppression by the combination of microbubble mediated ultrasound and immunotherapy2018

    • Author(s)
      Ryo Suzuki
    • Organizer
      6th International Symposium on focused Ultrasound
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 超音波照射下での気泡と細胞の相互作用:側方観察でわかったこと2018

    • Author(s)
      工藤信樹,磯野朱音
    • Organizer
      日本ソノケミストリー学会第10回超音波とファインバブルの_相互作用に関するシンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Importance to Consider Surrounding Condition of Microbubble to Elucidate Mechanisms of In Vivo Sonoporation2018

    • Author(s)
      Nobuki KUDO
    • Organizer
      High Intensity Focused Ultrasound: Mathematical Modeling & Clinical Applications
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] フォーカストシャドウグラフ法を用いたHIFU音場評価におけるカメラフォーカス位置の設定2018

    • Author(s)
      榊飛翔,工藤信樹
    • Organizer
      第16回日本超音波治療研究会(JSTU2017)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 電界誘起法により作製した微小気泡の超音波照射下でのふるまいの高速度観察2018

    • Author(s)
      常世 晶,工藤 信樹
    • Organizer
      第16回日本超音波治療研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ソノポレーション基礎検討のための電界誘起法による気泡発生手法の開発2018

    • Author(s)
      常世 晶,工藤 信樹
    • Organizer
      平成29年度 第4回超音波分子診断治療研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Cancer therapy by the combination of ultrasound contrast imaging agent and non-focused ultrasound induce cancer immune response2018

    • Author(s)
      Ryo Suzuki, Daiki Omata, Johan Unga, Lisa Munakata, Kazuo Maruyama
    • Organizer
      23rd European symposium on ultrasound contrast imaging
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] High-speed Observation of Microbubble Oscillation and Bubble-cell Interaction2017

    • Author(s)
      Nobuki KUDO
    • Organizer
      Artemino Conference
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Sonoporation using microbubbles: A technique for gene transduction and drug delivery2017

    • Author(s)
      Nobuki KUDO
    • Organizer
      BMEiCON 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Basic studies on mechanisms of sonoporation by high-speed observation of bubble-cell interaction under exposure to pulsed ultrasound2017

    • Author(s)
      Nobuki KUDO
    • Organizer
      Korean Society for Ultrasonic Therapy
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] High-speed Observation System Designed for Elucidating Mechanisms of Sonoporation2017

    • Author(s)
      Nobuki KUDO
    • Organizer
      Acoustic bubbles in therapy: recent advances with medical microbubbles, clouds and harmonic antibubbles
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Sonoporation using microbubbles: A technique for gene transduction and drug delivery2017

    • Author(s)
      Nobuki KUDO
    • Organizer
      International Conference on Biomedical Ultrasound 2017
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Microscopic Observation of Bubble-cell Interactions During Sonoporation2017

    • Author(s)
      Nobuki KUDO
    • Organizer
      2017 International Symposium of Frontier Acoustics
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] A simple technique for evaluation of a high-intensity focused ultrasound field using focused shadowgraphy2017

    • Author(s)
      Tsubasa Sakaki and Nobuki Kudo.
    • Organizer
      The 38th Symposium on Ultrasonic Electronics (USE2017)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 3D-Tissues on A Chip for Drug Assessment2017

    • Author(s)
      Michiya Matsusaki
    • Organizer
      2017ISOMRM
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Integration of Nano/Micro-Scaffolds and Cells for 3D-Tissue Construction2017

    • Author(s)
      Michiya Matsusaki
    • Organizer
      TERMIS-AP
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 臓器チップによる創薬支援技術の開発2017

    • Author(s)
      松崎典弥
    • Organizer
      日本機械学会イノベーション講演会(招待講演)
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 超音波セラノスティクス構築に向けた機能性微小気泡の開発2017

    • Author(s)
      鈴木 亮、小俣大樹、ウンガヨハン、丸山一雄
    • Organizer
      日本バイオイメージング学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Book] Biomaterials for Organ and Tissue Regeneration New Technologies and Future Prospects2020

    • Author(s)
      Figarol Agathe, Matsusaki Michiya
    • Total Pages
      846
    • Publisher
      Elsevier Science & Technology
    • ISBN
      9780081029060
    • Related Report
      2019 Annual Research Report
  • [Book] Fabrication methods of sustainable hydrogels, Sustainable Polymer Composites and Nanocomposites2019

    • Author(s)
      Cedric Delattre, Fiona Louis, Mitsuru Akashi, Michiya Matsusaki, Philippe Michaud, Guillaume Pierre
    • Total Pages
      1440
    • Publisher
      Springer
    • ISBN
      9783030053987
    • Related Report
      2018 Annual Research Report
  • [Book] 3次元生体組織モデルの構築および薬剤効果判定・毒性評価への応用, 再生医療・創薬のための3次元細胞培養技術2018

    • Author(s)
      松崎典弥, 高木大輔,瀬尾 学,宮川 繁,澤 芳樹,明石 満
    • Total Pages
      203
    • Publisher
      シーエムシー出版
    • ISBN
      9784781313306
    • Related Report
      2018 Annual Research Report
  • [Book] 第II編 要素技術 第5章 高分子化学に基づく三次元組織構築, 臓器チップの技術と開発動向2018

    • Author(s)
      松崎典弥
    • Total Pages
      293
    • Publisher
      シーエムシー出版
    • ISBN
      9784781313252
    • Related Report
      2018 Annual Research Report
  • [Book] The Micro-World Observed by Ultra High-Speed Cameras: We See What You Don’t See2017

    • Author(s)
      Tsuji, Kinko (Ed.)
    • Total Pages
      415
    • Publisher
      Springer
    • ISBN
      9783319614915
    • Related Report
      2017 Annual Research Report
  • [Book] Skin Permeation and Disposition of Therapeutic and Cosmeceutical Compounds2017

    • Author(s)
      M. Matsusaki, K. Fujimoto, Y. Shirakata, S. Hirakawa, K. Hashimoto, M. Akashi
    • Total Pages
      428
    • Publisher
      Springer-Nature
    • Related Report
      2017 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞外マトリックス含有組成物、三次元組織体形成用仮足場材及び三次元組織体形成剤2020

    • Inventor(s)
      松崎典弥・フィガロルアガットエリザベスマリー・佐藤薫
    • Industrial Property Rights Holder
      国立大学法人大阪大学・国立医薬品食品衛生研究所
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 細胞外マトリックス含有組成物及びその製造方法、並びに三次元組織体及びその製造方法2020

    • Inventor(s)
      北野史朗・入江新司・松崎典弥
    • Industrial Property Rights Holder
      国立大学法人大阪大学・凸版印刷株式会社
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 三次元組織体及びその製造方法並びに細胞含有組成物の製造方法2020

    • Inventor(s)
      北野史朗・入江新司・松崎典弥
    • Industrial Property Rights Holder
      国立大学法人大阪大学・凸版印刷株式会社
    • Industrial Property Rights Type
      特許
    • Filing Date
      2020
    • Related Report
      2019 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 細胞構造体及び細胞構造体の製造方法2019

    • Inventor(s)
      北野史朗・入江新司・ルイス フィオナ・松崎典弥
    • Industrial Property Rights Holder
      北野史朗・入江新司・ルイス フィオナ・松崎典弥
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-070137
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 三次元組織体及びその製造方法並びに細胞含有組成物の製造方法2019

    • Inventor(s)
      北野史朗・入江新司・松崎典弥
    • Industrial Property Rights Holder
      北野史朗・入江新司・松崎典弥
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-069972
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞外マトリックス含有組成物及びその製造方法、並びに三次元組織体及びその製造方法2019

    • Inventor(s)
      北野史朗・入江新司・松崎典弥
    • Industrial Property Rights Holder
      北野史朗・入江新司・松崎典弥
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-069976
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞外マトリックス含有組成物、三次元組織体形成用仮足場材及び三次元組織体形成剤2018

    • Inventor(s)
      松崎典弥・北野史朗・入江新司
    • Industrial Property Rights Holder
      松崎典弥・北野史朗・入江新司
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-087321
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] 細胞外マトリックス含有組成物及びその製造方法、並びに三次元組織体、三次元組織体形成剤2018

    • Inventor(s)
      松崎典弥・北野史朗・入江新司
    • Industrial Property Rights Holder
      松崎典弥・北野史朗・入江新司
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-087326
    • Filing Date
      2018
    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] 三次元組織体及びその製造方法、並びに、三次元組織体の形2017

    • Inventor(s)
      松崎典弥・入江新 司・北野史朗
    • Industrial Property Rights Holder
      松崎典弥・入江新 司・北野史朗
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2017-169834
    • Filing Date
      2017
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-01-27  

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