| Budget Amount *help |
¥44,850,000 (Direct Cost: ¥34,500,000、Indirect Cost: ¥10,350,000)
Fiscal Year 2019: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2018: ¥10,790,000 (Direct Cost: ¥8,300,000、Indirect Cost: ¥2,490,000)
Fiscal Year 2017: ¥23,270,000 (Direct Cost: ¥17,900,000、Indirect Cost: ¥5,370,000)
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| Outline of Final Research Achievements |
For PAS and rhodopsin proteins, we observed the structural changes taking place at multiple functional sites in the process from the triggering change to the key step of biological function, and clarified the linkage between the multiple functional sites by using time-resolved resonance Raman spectroscopy. We also quantitatively examined the correlations between the clarified coupled conformational changes and the functional activity of the proteins. The observed correlations identified which conformational changes are key to the protein functions. Furthermore, based on the identification of key structural changes, the creation of chimeric proteins with novel functions has elucidated the interlocking mechanisms that produce molecular functions.
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