| Budget Amount *help |
¥44,980,000 (Direct Cost: ¥34,600,000、Indirect Cost: ¥10,380,000)
Fiscal Year 2020: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2019: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2017: ¥26,130,000 (Direct Cost: ¥20,100,000、Indirect Cost: ¥6,030,000)
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| Outline of Final Research Achievements |
Two transformants of Cupriavidus sp. KN1 having trichloroethene degradability were utilized to investigate the formation of predation resistance to a protist. These two strains (StrainA and StrainB) could selectively colonize on antibiotic-containing agar media. A continuous culture device was operated by using StrainA. The device consisted of two connected completely mixed reactors. StrainA was cultivated in the first reactor to undergo predation by a bacterivorous protist in the second reactor. The effluent of the second reactor contained residual StrainA and the protist. This effluent was supplemented with StrainB not in contact with the protist to perform comparative evaluations of the two strains as prey. Consequently, it was found that the protist-untouched StrainB were predated preferentially, and StrainA survived significantly. Thus, the bacterial grazing resistance was developed in the presence of a protist.
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