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Development of surface modification technology using a bacterial adhesive protein

Research Project

Project/Area Number 17H01345
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biofunction/Bioprocess
Research InstitutionNagoya University

Principal Investigator

Hori Katsutoshi  名古屋大学, 工学研究科, 教授 (50302956)

Co-Investigator(Kenkyū-buntansha) 加藤 竜司  名古屋大学, 創薬科学研究科, 准教授 (50377884)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥44,590,000 (Direct Cost: ¥34,300,000、Indirect Cost: ¥10,290,000)
Fiscal Year 2019: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2018: ¥12,090,000 (Direct Cost: ¥9,300,000、Indirect Cost: ¥2,790,000)
Fiscal Year 2017: ¥21,450,000 (Direct Cost: ¥16,500,000、Indirect Cost: ¥4,950,000)
Keywords接着蛋白質 / 細胞接着 / 微生物 / 表面 / 分子固定 / 生体材料 / 蛋白質 / バイオテクノロジー / 接着 / 生物・生体工学 / 生体分子 / タンパク質 / 原子間力顕微鏡 / 分子動力学シミュレーション / 相互作用 / 機能表面 / 接着状態 / 抗菌
Outline of Final Research Achievements

A rapid and versatile immobilization method using an adhesive protein for peptides, proteins, and liposomes onto material surfaces was developed. Furthermore, we clarified the effects of the adhesive protein-coated surfaces on cell adhesion and proliferation. The adhesion of the adhesive protein to the material surface was observed in water by AFM, measured by quartz crystal microbalance (QCM), and analyzed by all-atom molecular dynamics (MD) calculations to gain insight into the adhesion modes.

Academic Significance and Societal Importance of the Research Achievements

本研究で確立した表面修飾技術は、AtaAに機能性ペプチドやタンパク質、リポソームなどを融合し、化学修飾をしていない任意の材料基盤に水中接触させるだけで固定化するという、迅速かつ簡便な産業界待望のものである。また、本研究で明らかになったAtaAの接着様式は、生体分子-非生物表面間相互作用の原理解明と制御に向けて非常に重要な知見である。

Report

(4 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (38 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (7 results) Journal Article (7 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (20 results) (of which Int'l Joint Research: 9 results,  Invited: 8 results) Book (2 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 2 results)

  • [Int'l Joint Research] マックスプランク研究所/フランクフルト大学(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] オスロ大学(ノルウェー)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Nottingham Trent University(英国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] マックスプランク研究所/フランクフルト大学(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] オスロ大学(ノルウェー)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Max Planck Institute/Goethe University(ドイツ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] The University of Oslo(ノルウェー)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Native display of a huge homotrimeric protein fiber on the cell surface after precise domain deletion2020

    • Author(s)
      Aoki Sota、Yoshimoto Shogo、Ishikawa Masahito、Linke Dirk、Lupas Andrei、Hori Katsutoshi
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 129 Issue: 4 Pages: 412-417

    • DOI

      10.1016/j.jbiosc.2019.09.022

    • NAID

      40022216066

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Bottom-up Creation of an Artificial Cell Covered with the Adhesive Bacterionanofiber Protein AtaA2019

    • Author(s)
      Noba Kosaku、Ishikawa Masahito、Uyeda Atsuko、Watanabe Takayoshi、Hohsaka Takahiro、Yoshimoto Shogo、Matsuura Tomoaki、Hori Katsutoshi
    • Journal Title

      Journal of the American Chemical Society

      Volume: 141 Issue: 48 Pages: 19058-19066

    • DOI

      10.1021/jacs.9b09340

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Control of AtaA-mediated bacterial immobilization by casein hydrolysates2019

    • Author(s)
      Y. Ohara, S. Yoshimoto, K. Hori
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 128 Issue: 5 Pages: 544-550

    • DOI

      10.1016/j.jbiosc.2019.04.019

    • NAID

      40022069844

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Acinetobacter trimeric autotransporter adhesin Ata controls key virulence traits of Acinetobacter baumannii2019

    • Author(s)
      Marko Weidensdorfer, Masahito Ishikawa, Katsutoshi Hori, Dirk Linke, Bardya Djahanschiri, Ruben Iruegas, Ingo Ebersberger, Sara Riedel-Christ, Giulia Enders, Laura Leukert, Peter Kraiczy, Florian Rothweiler, Jindrich Cinatl, Jürgen Berger, Katharina Hipp, Volkhard AJ Kempf, Stephan Göttig
    • Journal Title

      Virulence

      Volume: 10 Issue: 1 Pages: 68-81

    • DOI

      10.1080/21505594.2018.1558693

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cell behavior of the highly sticky bacterium Acinetobacter sp. Tol 5 during adhesion in laminar flows2018

    • Author(s)
      Y. Furuichi, K. Iwasaki, and K. Hori
    • Journal Title

      Sci. Rep.

      Volume: 8 Issue: 1 Pages: 8285-8285

    • DOI

      10.1038/s41598-018-26699-5

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Immobilization of Enterobacter aerogenes by a trimetric autotransporter adhesin, AtaA, and its application to biohydrogen production2018

    • Author(s)
      H. Nakatani, N. Ding, Y. Ohara, and K. Hori
    • Journal Title

      Catalysts

      Volume: 8 Issue: 4 Pages: 159-159

    • DOI

      10.3390/catal8040159

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Integrated Production and Separation2017

    • Author(s)
      Hori K.、Unno H.
    • Journal Title

      Reference Module in Life Science

      Volume: - Pages: 579-590

    • DOI

      10.1016/b978-0-12-809633-8.09091-9

    • ISBN
      9780128096338
    • Related Report
      2017 Annual Research Report
  • [Presentation] 正確なドメイン欠損による巨大ファイバータンパク質の再構成2020

    • Author(s)
      青木壮太, 吉本将悟, 石川聖人, 堀 克敏
    • Organizer
      化学工学会第85年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 発現ホストの違いによる高凝集性タンパク質AtaAの付着凝集特性の相違2020

    • Author(s)
      岩崎啓太, 吉本将悟, 堀 克敏
    • Organizer
      化学工学会第85年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Inhibition and detachment of AtaA-mediated bacterial adhesion by casein hydrolysates2019

    • Author(s)
      Y. Ohara, S.Yoshimoto, K. Hori
    • Organizer
      The 25th Young Asian Biological Engineers’ Community
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Adheasion force mapping of cell surface of high adhesive bacteria using atomic force microscopy2019

    • Author(s)
      S. Ishii, S. Yoshimoto, K. Hori
    • Organizer
      The 25th Young Asian Biological Engineers’ Community
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Comparison of the adhesiveness of Acinetobacter sp. Tol 5 among various material surfaces2019

    • Author(s)
      A. Kawashiri, S. Yoshimoto, T. Matsushita, K. Iio, M. Takai, K. Hori
    • Organizer
      The 18th Asian Pacific Confederation of Chemical Engineering Congress
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] A new aspect of the translocator complex of trimeric autotransporter adhesins of Gram-negative bacterial2019

    • Author(s)
      K. Hori
    • Organizer
      Talking with membranes: biogenesis, communications and diseases
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] バクテリオナノファイバー蛋白質AtaAで修飾した接着性人工細胞の創出2019

    • Author(s)
      野場考策, 石川聖人, 植田淳子, 渡邉貴嘉, 芳坂貴弘, 吉本将悟, 松浦友亮, 堀 克敏
    • Organizer
      第9回CSJ化学フェスタ
    • Related Report
      2019 Annual Research Report
  • [Presentation] 分子動力学計算による高接着性タンパク質AtaA_Nheadの接着過程解析2019

    • Author(s)
      笹原純, 鈴木淳巨, 藤本和士, 岡崎 進, 堀 克敏
    • Organizer
      第9回CSJ化学フェスタ
    • Related Report
      2019 Annual Research Report
  • [Presentation] AFMを用いた高付着性細菌及びその細胞表層タンパク質の接着力測2019

    • Author(s)
      石井慧,吉本将悟,堀 克敏
    • Organizer
      第71回日本生物工学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] バクテリオナノファイバー蛋白質AtaAで修飾した接着性人工細胞の創出2019

    • Author(s)
      野場考策,石川聖人,植田淳子,渡邉貴嘉,芳坂貴弘,吉本将悟,松浦友亮,堀 克敏
    • Organizer
      第13回バイオ関連化学シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] 高付着性蛋白質AtaAで被覆された人工細胞のボトムアップ創成2019

    • Author(s)
      野場考策,石川聖人,植田淳子,渡邊貴嘉,芳坂貴弘,吉本将悟,松浦友亮,堀 克敏
    • Organizer
      2019年度 生物工学会中部支部例会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Searching inhibitors against adhesion of the nanofiber protein AtaA and the control of microbial immobilization2018

    • Author(s)
      Y. Ohara, S. Yoshimoto, K. Hori
    • Organizer
      iBio-N 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Distant variable peptide display system on bacterial cell utilizing long bacterionanofiber of a trimeric autotransporter adhesin2018

    • Author(s)
      H. Nakatani, K. Hori
    • Organizer
      iBio-N 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of an efficient counterselection method for Methylococcus capsulatus (Bath)2018

    • Author(s)
      M. Ishikawa, K. Hori
    • Organizer
      Asian Synthetic Biology Association 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 微生物バクテリオナノファイバーによる微生物プロセスの革新2018

    • Author(s)
      堀 克敏
    • Organizer
      環境バイオテクノロジー学会2018年度大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 水中強接着性細菌タンパク質AtaAの非特異的相互作用の謎に迫る2018

    • Author(s)
      堀 克敏
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Protein engineering of bacterionanofiber protein for the innovative bioprocesses2018

    • Author(s)
      K. Hori
    • Organizer
      Asian Synthetic Biology Association 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Quantification of cell adhesion of Acinetobacter sp. Tol 5 via a trimeric autotransporter adhesin using atomic force microscopy2017

    • Author(s)
      S. Ishii, S. Yoshimoto, K. Hori
    • Organizer
      FEMS 7th Congress of European Microbiologists
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] AtaA, a new trimeric autotransporter adhesin mediating strong and nonspecific adhesion of bacterial cells2017

    • Author(s)
      K. Hori
    • Organizer
      第90回日本細菌学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 付着性・凝集性細菌の付着・バイオフィルム形成過程と固定化技術の発展,化学工学会2017

    • Author(s)
      堀 克敏
    • Organizer
      第49回化学工学会度秋季大会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Book] 細胞表層工学の進展2020

    • Author(s)
      堀 克敏
    • Total Pages
      246
    • Publisher
      シーエムシー出版
    • ISBN
      9784781314969
    • Related Report
      2019 Annual Research Report
  • [Book] 細胞・生体分子の固定化と機能発現2018

    • Author(s)
      堀 克敏
    • Total Pages
      294
    • Publisher
      シーエムシー出版
    • ISBN
      9784781313269
    • Related Report
      2018 Annual Research Report
  • [Patent(Industrial Property Rights)] 分離ペプチド2019

    • Inventor(s)
      堀 克敏
    • Industrial Property Rights Holder
      名古屋大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-132490
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 接着タンパク質2019

    • Inventor(s)
      堀 克敏
    • Industrial Property Rights Holder
      名古屋大学
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2019-132488
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
    • Overseas

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Published: 2017-04-28   Modified: 2024-01-30  

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