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Development of the energy recovery technology from a depleted oilfield that tailors geochemical and microbiological characteristics of the oil reservoir

Research Project

Project/Area Number 17H01363
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Earth system and resources engineering
Research InstitutionNational Institute of Advanced Industrial Science and Technology

Principal Investigator

Susumu Sakata  国立研究開発法人産業技術総合研究所, 地質調査総合センター, 招聘研究員 (70357101)

Co-Investigator(Kenkyū-buntansha) 玉木 秀幸  国立研究開発法人産業技術総合研究所, 生命工学領域, 研究グループ長 (00421842)
眞弓 大介  国立研究開発法人産業技術総合研究所, 地質調査総合センター, 主任研究員 (30549861)
Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥43,680,000 (Direct Cost: ¥33,600,000、Indirect Cost: ¥10,080,000)
Fiscal Year 2020: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2019: ¥10,660,000 (Direct Cost: ¥8,200,000、Indirect Cost: ¥2,460,000)
Fiscal Year 2018: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
Fiscal Year 2017: ¥12,220,000 (Direct Cost: ¥9,400,000、Indirect Cost: ¥2,820,000)
Keywords国内油田 / 原油分解メタン生成 / 微生物攻法 / バイオオーグメンテーション / バイオスティミュレーション / エネルギー増進回収 / 高温高圧培養実験 / 油層 / 環境微生物 / 原油分解 / メタン生成 / 酵母エキス / トルエン / 長鎖ノルマルアルカン / 枯渇油田 / 微生物 / エネルギー回収 / 地球化学 / 微生物学 / 分子生態学 / 原油分解メタン生成ポテンシャル / 安定培養化 / 枯渇油田再生 / 東北日本油ガス田 / 油層微生物 / 原油生分解 / メタン生成ポテンシャル / 炭化水素組成炭素同位体比 / 資源開発 / 原油 / メタン
Outline of Final Research Achievements

We performed laboratory incubation experiments mimicking temperature and pressure conditions of the oil reservoir to evaluate the feasibility of microbial enhanced energy recovery from a depleted oilfield. A microbial community capable of methanogenic crude oil degradation was successfully obtained from the production water of Yamagata oilfield, whose methanogenic crude oil degradation potential had been indicated from geochemical analyses. Addition of this microbial culture to the production water from Akita oilfield with no geochemical signature of the oil degradation potential, together with a specific yeast extract, resulted in a large amount of methane production coupled to toluene and n-alkane degradation during the incubation. Our result demonstrates the effectiveness of energy recovery technology from a depleted oilfield that tailors geochemical characteristics of the oil reservoir for applying biological augmentation and/or stimulation.

Academic Significance and Societal Importance of the Research Achievements

本研究成果の学術的意義は、世界に先駆けて、油層環境を模擬する高温高圧条件で現場の微生物を培養し、原油からメタンが生成されるプロセスの再現に成功したことである。またバイオオーグメンテーションにより原油分解活性のない油層に同活性を誘起できることを実証したのも本研究が初めてである。本研究が志向する枯渇油田のエネルギー回収技術は、国内電力生産の3割以上を石油や天然ガスに依存する日本において新たなエネルギー資源の創成に導くものである。現場環境を模擬する培養試験でバイオオーグメンテーションの有効性を実証できたことは、本資源技術の実用化に向けた大きな前進であり、社会的ニーズに対応する研究成果である。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (26 results)

All 2021 2020 2019 2018 2017

All Journal Article (13 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 13 results,  Open Access: 7 results) Presentation (13 results) (of which Int'l Joint Research: 6 results,  Invited: 4 results)

  • [Journal Article] Sakamoto S, Nobu MK, Mayumi D, Tamazawa S, Kusada H, Yonebayashi H, Iwama H, Ikarashi M, Wakayama T, Maeda H, Sakata S, Tamura T, Nomura N,2021

    • Author(s)
      Sakamoto S, Nobu MK, Mayumi D, Tamazawa S, Kusada H, Yonebayashi H, Iwama H, Ikarashi M, Wakayama T, Maeda H, Sakata S, Tamura T, Nomura N,
    • Journal Title

      Syst. Appl. Microbiol.

      Volume: 44 Issue: 1 Pages: 126154-126154

    • DOI

      10.1016/j.syapm.2020.126154

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Methanogenic crude oil degradation induced by an exogenous microbial community and nutrient injections2021

    • Author(s)
      Suda Konomi、Ikarashi Masayuki、Tamaki Hideyuki、Tamazawa Satoshi、Sakata Susumu、Haruo Maeda、Kamagata Yoichi、Kaneko Masanori、Ujiie Tomomi、Shinotsuka Yumi、Wakayama Tatsuki、Iwama Hiroki、Osaka Noriko、Mayumi Daisuke、Yonebayashi Hideharu
    • Journal Title

      Journal of Petroleum Science and Engineering

      Volume: 201 Pages: 108458-108458

    • DOI

      10.1016/j.petrol.2021.108458

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Unexpected diversity of acetate degraders in anaerobic membrane bioreactor treating organic solid waste revealed by high-sensitivity stable isotope probing2020

    • Author(s)
      Tomo Aoyagi, Tomohiro Inaba, Hidenobu Aizawa, Daisuke Mayumi, Susumu Sakata, Amine Charfi, Changwon Suh, Jong Hong Lee, Yuya Sato, Atsushi Ogata, Hiroshi Habe, Tomohiro Hori
    • Journal Title

      Water Research

      Volume: 176 Pages: 115750-115750

    • DOI

      10.1016/j.watres.2020.115750

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Microbial methane production and oxidation in the Holocene mud beneath the Kanto Plain of central Japan2020

    • Author(s)
      Hideyoshi Yoshioka, Mio Takeuchi, Susumu Sakata, Hiroshi A. Takahashi, Manabu Takahashi, Susumu Tanabe, Takeshi Hayashi, Akihiko Inamura, Masaya Yasuhara
    • Journal Title

      Geochemical Journal

      Volume: 54 Issue: 4 Pages: 243-254

    • DOI

      10.2343/geochemj.2.0597

    • NAID

      130007891325

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cultivation Renaissance in the Post-Metagenomics Era: Combining the New and Old2019

    • Author(s)
      Tamaki Hideyuki
    • Journal Title

      Microbes and Environments

      Volume: 34 Issue: 2 Pages: 117-120

    • DOI

      10.1264/jsme2.ME3402rh

    • NAID

      130007668647

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Improvement of terrestrial groundwater sampling method affects microbial community analysis2019

    • Author(s)
      Akio Ueno, Satoshi Tamazawa, Shuji Tamamura, Noritaka Aramaki, A. K. M. Alam Badrul, Takuma Murakami, Shinji Yamaguchi, Junya Yamagishi, Hideyuki Tamaki, Daisuke Mayumi, Takeshi Naganuma & Katsuhiko Kaneko
    • Journal Title

      Geomicrobiology Journal

      Volume: 36 Issue: 4 Pages: 303-316

    • DOI

      10.1080/01490451.2018.1534900

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Diversity and geochemical community assembly processes of the living rare biosphere in a sand-and-gravel aquifer ecosystem in the Midwestern United States2019

    • Author(s)
      Yamamoto K, Hackley CK, Kelly RW, Panno VS, Sekiguchi Y, Sanford RA, Liu WT, Kamagata Y, Tamaki H
    • Journal Title

      Sci. Rep.

      Volume: 9 Issue: 1 Pages: 1-11

    • DOI

      10.1038/s41598-019-49996-z

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Microbial community structure in deep natural gas-bearing aquifers subjected to sulfate-containing fluid injection2019

    • Author(s)
      Katayama Taiki、Yoshioka Hideyoshi、Yamanaka Toshiro、Takeuchi Mio、Muramoto Yoshiyuki、Usami Jun、Ikeda Hidefumi、Sakata Susumu
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 127 Issue: 1 Pages: 45-51

    • DOI

      10.1016/j.jbiosc.2018.06.013

    • NAID

      40021783370

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible cross-feeding pathway of facultative methylotroph Methyloceanibacter caenitepidi Gela4 on methanotroph Methylocaldum marinum S8.2019

    • Author(s)
      Mio Takeuchi, Haruka Ozaki, Satoshi Hiraoka, Yoichi Kamagata, Susumu Sakata, Hideyoshi Yoshioka, and Wataru Iwasaki.
    • Journal Title

      PLOS ONE

      Volume: 14 Issue: 3 Pages: e0213535-e0213535

    • DOI

      10.1371/journal.pone.0213535

    • NAID

      120007128121

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 天然ガス・石油の成因と微生物の寄与に関する有機地球化学的研究2018

    • Author(s)
      坂田将
    • Journal Title

      Researches in Organic Geochemistry

      Volume: 34 Pages: 1-13

    • NAID

      130007607929

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 微生物原油分解EORフィールドパイロットに向けて~微生物培養実験からパイロット計画策定まで~2018

    • Author(s)
      岩間弘樹,五十嵐雅之,若山樹,米林英治,眞弓大介,前田治男,須田好,玉木秀幸,坂田将,鎌形洋一
    • Journal Title

      石油技術協会誌

      Volume: 83 Pages: 455-460

    • NAID

      130007966700

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Petrothermobacter organivorans gen. nov., sp. nov., a novel thermophilic, strictly anaerobic bacterium of the phylum Deferribacteres isolated from a deep subsurface oil reservoir2017

    • Author(s)
      73.Tamazawa S, Mayumi D, Mochimaru H, Sakata S, Maeda H, Wakayama T, Ikarashi M, Kamagata Y, Tamaki H
    • Journal Title

      Int. J. Syst. Evol. Microbiol.

      Volume: 67 Issue: 10 Pages: 3982-3986

    • DOI

      10.1099/ijsem.0.002234

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Origin of methane-rich natural gas at the West Pacific convergent plate boundary2017

    • Author(s)
      Sano Y., Kinoshita N., Kagoshima T., Takahata N., Sakata S., Toki T., Kawagucci S., Waseda A., Lan T., Wen H., Chen A., Yang T. F., Guodong Z., Tomonaga Y., Roulleau E., Pinti D. L.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 15646-15646

    • DOI

      10.1038/s41598-017-15959-5

    • NAID

      120006383299

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 深部地下圏でメトキシ芳香族化合物分解を担う新規共生細菌の生存戦略2021

    • Author(s)
      坂本幸子、Nobu Masaru Konishi、眞弓大介、玉澤聡、中原望、草田裕之、米林英治、岩間弘樹、五十嵐雅之、若山樹、前田治男、坂田将、田村具博、野村暢彦、鎌形洋一、玉木秀幸
    • Organizer
      日本農芸化学会2021年度大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 深部地下圏でメトキシ芳香族化合物分解を担う新規共生細菌の発見及び生存戦略の解明2021

    • Author(s)
      坂本幸子、Nobu Masaru Konishi、眞弓大介、玉澤聡、中原望、草田裕之、米林英治、岩間弘樹、五十嵐雅之、若山樹、前田治男、坂田将、田村具博、野村暢彦、鎌形洋一、玉木秀幸
    • Organizer
      第15回日本ゲノム微生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Potential and mechanisms of methane production by subsurface microorganisms in oil and gas fields.2019

    • Author(s)
      Susumu Sakata
    • Organizer
      8th Congress of European Microbiologists (FEMS 2019)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Enhancement of methanogenic crude oil degaradation using organic nutrients toward biotechnology applications in oil reservoirs.2019

    • Author(s)
      Daisuke Mayumi, Konomi Suda, Hideyuki Tamaki, Haruo Maeda, Yoichi Kamagata, Susumu Sakata, Hiroki Iwama, Masayuki Ikarashi, Tatsuki Wakayama and Hideharu Yonebayashi
    • Organizer
      7th International Symposium on Applied Microbiology and Molecular Biology in Oil Systems
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Field Application of Bio-Augmentation processes for Microbial Enhance Oil Recovery Targeting Depleted Oil Field2019

    • Author(s)
      Hiroki Iwama, Masayuki Ikarashi, Tatsuki Wakayama, Hideharu Yonebayashi, Daisuke Mayumi, Haruo Maeda, Konomi Suda, Hideyuki Tamaki, Susumu Sakata, Youichi Kamagata
    • Organizer
      7th International Symposium on Applied Microbiology and Molecular Biology in Oil Systems
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 地下微生物が石炭をメタンに変換するポテンシャルとメカニズム2019

    • Author(s)
      坂田 将
    • Organizer
      平成30年度石油技術協会春季講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 天然ガスや石油の根源物質としての地下圏有機物2019

    • Author(s)
      坂田 将
    • Organizer
      日本腐植物質学会第 35 回講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 石炭を直接利用するメタン菌の新たなメタン生成経路2018

    • Author(s)
      眞弓大介, 持丸華子, 玉木秀幸, 山本京祐, 吉岡秀佳, 鈴木祐一郎, 鎌形洋一, 坂田将
    • Organizer
      日本地球化学会第65回年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 微生物原油分解EORフィールドパイロットに向けて~微生物培養実験からパイロット計画策定まで~2018

    • Author(s)
      岩間弘樹,五十嵐雅之,若山樹,米林英治,眞弓大介,前田治男,須田好,玉木秀幸,坂田将,鎌形洋一
    • Organizer
      平成30年度石油技術協会春季講演会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Realization of ULTRG (Unrecoverable Liquid fuels To Recoverable Gaseous fuels) as an innovative Microbial EOR2017

    • Author(s)
      前田治男,五十嵐雅之,眞弓大介,坂田将,飯田剛史,大坂典子
    • Organizer
      2017年SPEサウジアラビア支部年会
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Realization of subsurface methanogenic oil degradation system― Important role of porous environment and nutrients2017

    • Author(s)
      五十嵐雅之,若山樹,前田治男,米林英治,飯田剛史,大坂典子,眞弓大介,玉澤聡,玉木秀幸,坂田将,鎌形洋一
    • Organizer
      6th International Symposium on Applied Microbiology and Molecular Biology in Oil Systems
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Geochemical analyses can contribute to search hydrocarbon degrading microbes for new conceptual enhanced oil recovery2017

    • Author(s)
      米林英治,前田治男,五十嵐雅之,若山樹,眞弓大介,玉澤聡,玉木秀幸,坂田将,白井良和,大坂典子
    • Organizer
      28th International Meeting on Organic Geochemistry
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 原油分解メタン生成のフィールドへの適用に向けた検討2017

    • Author(s)
      岩間弘樹,五十嵐雅之,若山樹,前田治男,米林英治,眞弓大介,玉木秀幸,坂田将,鎌形洋一,飯田剛史,五味保城
    • Organizer
      2017年石油学会秋季討論会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2022-03-04  

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