Mechanism of pre- and post-synaptic interactions
| Project/Area Number |
17H01387
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nerve anatomy/Neuropathology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
OKABE SHIGEO 東京大学, 大学院医学系研究科(医学部), 教授 (60204012)
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| Co-Investigator(Kenkyū-buntansha) |
原田 彰宏 大阪大学, 医学系研究科, 教授 (40251441)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000)
Fiscal Year 2019: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2018: ¥13,520,000 (Direct Cost: ¥10,400,000、Indirect Cost: ¥3,120,000)
Fiscal Year 2017: ¥14,950,000 (Direct Cost: ¥11,500,000、Indirect Cost: ¥3,450,000)
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| Keywords | イメージング / シナプス形成 / 神経回路 / スパイン / 分子神経生物学 |
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| Outline of Final Research Achievements |
Neuronal synapses express their function triggered by binding of presynaptic structure, a structural component of the axon, and post-synaptic structure, a part of dendritic compartment accumulating neurotransmitter receptors. In this study, we studied how the interaction between pre- and post-synaptic components regulates synaptic functions, development, and remodeling. One of our significant findings is the identification of BMP4 as a factor that is secreted from the axon and negatively regulates synapse formation and stabilization. We also optimized the structured illumination microscopy and image processing techniques for the realization of quantitative 3D analysis of dendritic spines. The method was applied to structural remodeling of dendritic spines associated with neuronal activity.
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| Academic Significance and Societal Importance of the Research Achievements |
脳内での神経回路の発達はヒトでは生後の数年間に起こり、その障害は自閉スペクトラム症などの疾患に深く関与するが神経回路発達の制御機構には不明の点が多い。神経回路の発達は軸索と樹状突起が接触してシナプスを形成することによって進む。本研究では神経細胞の二つの異なる突起である軸索と樹状突起の接触によってシナプスの形成と安定化がどのように制御されるのかを解析し、軸索から放出される因子や樹状突起側に作られる微小な突起（スパイン）の頭部に形成される軸索との特徴的な接触構造が重要な制御要素であることを明らかにした。
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Report
(4 results)
Research Products
(66 results)
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[Journal Article] LAMP5 in presynaptic inhibitory terminals in the hindbrain and spinal cord: a role in startle response and auditory processing2019
Author(s)
.Koebis, M., Urata, S., Shinoda, Y., Okabe, S., Yamasoba, T., Nakao, K., Aiba, A., and T. Furuichi,
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Journal Title
Molecular Brain
Volume: 12
Issue: 1
Pages: 20-20
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Computational geometry analysis of dendritic spines by structured illumination microscopy2019
Author(s)
Kashiwagi, Y., Higashi, T., Obashi, K., Sato, Y., Komiyama, N., Grant, S. G. N. and S. Okabe
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Journal Title
Nature Communications
Volume: 10
Issue: 1
Pages: 1285-1285
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] The inflammasome-derived cytokine IL18 suppresses amyloid-induced seizures in Alzheimer’s prone mice2018
Author(s)
Tzeng, T-C, Hasegawa, Y., Iguchi, R., Caffrey, D. R., Thatcher, E. J., Mao, W.J., Germain, G., Tamburro, N. D., Okabe, S., Heneka, M., Latz, E., Futai, K.,and D. T. Golenbock
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 115
Issue: 36
Pages: 9002-9007
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] In vivo observation of structural changes in neocortical catecholaminergic projections in response to drugs of abuse2018
Author(s)
Morimoto, M. Tanaka, S., Mizutani, S., Urata, S., Kobayashi, K., Okae, S.
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Journal Title
eNeuro
Volume: 6
Issue: 1
Pages: 1-1
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Near-infrared deep brain stimulation via upconversion nanoparticle-mediated optogenetics.2018
Author(s)
Chen S, Weitemier AZ, Zeng X, He L, Wang X, Tao Y, Huang AJY, Hashimotodani Y, Kano M, Iwasaki H, Parajuli LK, Okabe S, Teh DBL, All AH, Tsutsui-Kimura I, Tanaka KF, Liu X, McHugh TJ.
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Journal Title
Science
Volume: 359
Issue: 6376
Pages: 679-684
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Serotonin rebalances cortical tuning and behavior linked to autism symptoms in 15q11-13 CNV mice.2017
Author(s)
Nakai N, Nagano M, Saitow F, Watanabe Y, Kawamura Y, Kawamoto A, Tamada K, Mizuma H, Onoe H, Watanabe Y, Monai H, Hirase H, Nakatani J, Inagaki H, Kawada T, Miyazaki T, Watanabe M, Sato Y, Okabe S, Kitamura K, Kano M, Hashimoto K, Suzuki H, Takumi T.
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Journal Title
Sci Adv.
Volume: 3(6)
Issue: 6
DOI
Related Report
Peer Reviewed / Open Access
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[Presentation] AiCE mouse: a novel tool to probe LTP-related change in dendritic spines2019
Author(s)
K. Kuboyama, T. Inoue, Y. Hashimotodani, T. Itoh, T. Suzuki, Y. Ohtsuka, R. Kinoshita, P. Gusain, M. Kano, S. Okabe, M.K. Yamada
Organizer
第42回日本神経科学大会
Related Report
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