Development of in vivo gene manipulation methods using CRISPR/Cas9 system

• Project/Area Number: 17H01394
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Laboratory animal science
• Research Institution: Osaka University
• Principal Investigator: IKAWA MASAHITO 大阪大学, 微生物病研究所, 教授 (20304066)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥41,730,000 (Direct Cost: ¥32,100,000、Indirect Cost: ¥9,630,000); Fiscal Year 2020: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000); Fiscal Year 2019: ¥11,440,000 (Direct Cost: ¥8,800,000、Indirect Cost: ¥2,640,000); Fiscal Year 2018: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000); Fiscal Year 2017: ¥10,010,000 (Direct Cost: ¥7,700,000、Indirect Cost: ¥2,310,000)
• Keywords: 実験動物 / ゲノム編集 / ウイルスベクター / CRISPR/Cas9 / 不妊 / CRISPR/cas9 / ES細胞

Outline of Final Research Achievements

We have developed an on-demand genome editing system that enables arbitrary genetic modification by combining CRISPR/Cas9 genome editing technology with lentiviral vectors and ES cells, which are the applicant's specialties. In addition, using the developed genome editing technology and animals, we conducted functional analysis of testis-specific expressed genes to clarify the mechanisms of spermatogenesis and acquisition of sperm function.

Academic Significance and Societal Importance of the Research Achievements

生命科学研究の進展には、個体レベルで遺伝子機能を解析する手法が必要不可欠である。申請者はCRISPR/Cas9ゲノム編集技術を活用して任意の遺伝子改変マウスを作製するツールを開発した。さらに、開発した遺伝子組換え技術を用いて雄性不妊マウスを多数作製し、その原因を明らかにした。本研究成果は、社会問題にもなっている不妊症メカニズム解明と治療法開発に貢献する。

Research Products

• [Int'l Joint Research] Baylor College of Medecine/University of Hawai(米国)
• [Int'l Joint Research] Seoul National University(韓国)
• [Int'l Joint Research] Institute of Molecular and Cell Biology(シンガポール)
• [Int'l Joint Research] Baylor College of Medecine/University of Hawai(米国)
• [Int'l Joint Research] Seoul National University(韓国)
• [Int'l Joint Research] Baylor College of Medecine/University of Hawai(米国)
• [Int'l Joint Research] Seoul National University(韓国)
• [Int'l Joint Research] Baylor College of Medecine/University of Hawaii(米国)
• [Int'l Joint Research] Seoul National University(韓国)
• [Journal Article] ARMC12 regulates spatiotemporal mitochondrial dynamics during spermiogenesis and is required for male fertility (2021)
  • Author(s): Shimada Keisuke、Park Soojin、Miyata Haruhiko、Yu Zhifeng、Morohoshi Akane、Oura Seiya、Matzuk Martin M.、Ikawa Masahito
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 118 Issue: 6 Pages: 1-12
  • DOI: 10.1073/pnas.2018355118
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] NAIL: an evolutionarily conserved lncRNA essential for licensing coordinated activation of p38 and NFκB in colitis. (2020)
  • Author(s): Akinciar SC, Wu L, Ng QF, Chua JYH, Unal B, Noda T, Chor WHJ, Ikawa M, Tergaonkar V.
  • Journal Title: Gut. Volume: gutjnl-2020 Issue: 10 Pages: 322980-322980
  • DOI: 10.1136/gutjnl-2020-322980
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CRISPR/CAS9-mediated amino acid substitution reveals phosphorylation residues of RSPH6A are not essential for male fertility in mice†. (2020)
  • Author(s): Miyata H, Abbasi F, Visconti PE, Ikawa M.
  • Journal Title: Biol Reprod. Volume: 103 Issue: 5 Pages: 912-914
  • DOI: 10.1093/biolre/ioaa161
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Knockout of family with sequence similarity 170 member A (Fam170a) causes male subfertility, while Fam170b is dispensable in mice†. (2020)
  • Author(s): Devlin DJ, Nozawa K, Ikawa M, *Matzuk MM.
  • Journal Title: Biol Reprod. Volume: 103 Issue: 2 Pages: 205-222
  • DOI: 10.1093/biolre/ioaa082
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CRISPR/Cas9-mediated genome-edited mice reveal 10 testis-enriched genes are dispensable for male fecundity (2020)
  • Author(s): Park Soojin、Shimada Keisuke、Fujihara Yoshitaka、Xu Zoulan、Shimada Kentaro、Larasati Tamara、Pratiwi Putri、Matzuk Ryan M、Devlin Darius J、Yu Zhifeng、Garcia Thomas X、Matzuk Martin M、Ikawa Masahito
  • Journal Title: Biology of Reproduction Volume: 103 Issue: 2 Pages: 195-204
  • DOI: 10.1093/biolre/ioaa084
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CRISPR/Cas9-based genome editing in mice uncovers 13 testis- or epididymis-enriched genes individually dispensable for male reproduction (2020)
  • Author(s): Sun Jiang、Lu Yonggang、Nozawa Kaori、Xu Zoulan、Morohoshi Akane、Castaneda Julio M、Noda Taichi、Miyata Haruhiko、Abbasi Ferheen、Shawki Hossam H、Takahashi Satoru、Devlin Darius J、Yu Zhifeng、Matzuk Ryan M、Garcia Thomas X、Matzuk Martin M、Ikawa Masahito
  • Journal Title: Biology of Reproduction Volume: N/A Issue: 2 Pages: 183-194
  • DOI: 10.1093/biolre/ioaa083
  • NAID: 120007190927
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CIB4 Is Essential for the Haploid Phase of Spermatogenesis in Mice (2020)
  • Author(s): Zoulan Xu, Haruhiko Miyata, Yuki Kaneda, Julio M Castaneda, Yonggang Lu, Akane Morohoshi, Zhifeng Yu, Martin M Matzuk, Masahito Ikawa
  • Journal Title: Biology of Reproduction Volume: 102 Issue: 2 Pages: 1-9
  • DOI: 10.1093/biolre/ioaa059
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Tmprss12 is required for sperm motility and uterotubal junction migration in mice (2020)
  • Author(s): §Larasati T., §Noda T., Fujihara Y., Shimada K., Tobita T., Yu Z., Matzuk M.M., Ikawa M.
  • Journal Title: Biology of Reproduction Volume: - Issue: 2 Pages: 254-263
  • DOI: 10.1093/biolre/ioaa060
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Prss55 but not Prss51 is required for male fertility in mice† (2020)
  • Author(s): Kobayashi Kiyonori、Endo Tsutomu、Matsumura Takafumi、Lu Yonggang、Yu Zhifeng、Matzuk Martin M、Ikawa Masahito
  • Journal Title: Biology of Reproduction Volume: - Issue: 2 Pages: 223-234
  • DOI: 10.1093/biolre/ioaa041
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Knockout of serine-rich single-pass membrane protein 1 (Ssmem1) causes globozoospermia and sterility in male mice†. (2020)
  • Author(s): Nozawa K, Zhang Q, Miyata H, Devlin DJ, Yu Z, Oura S, Koyano T, Matsuyama M, Ikawa M, Matzuk MM.
  • Journal Title: Biol Reprod. Volume: 103 Issue: 2 Pages: 244-253
  • DOI: 10.1093/biolre/ioaa040
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] NELL2-mediated lumicrine signaling through OVCH2 is required for male fertility. Science. (2020)
  • Author(s): Kiyozumi D, Noda T, Yamaguchi R, Tobita T, Matsumura T, Shimada K, Kodani M, Kohda T, Fujihara Y, Ozawa M, Yu Z, Miklossy G, Bohren KM, Horie M, Okabe M, Matzuk MM, Ikawa M.
  • Journal Title: Science. Volume: 368 Issue: 6495 Pages: 1132-1135
  • DOI: 10.1126/science.aay5134
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sperm proteins SOF1, TMEM95, and SPACA6 are required for sperm－oocyte fusion in mice (2020)
  • Author(s): Taichi Noda, Yonggang Lu, Yoshitaka Fujihara, View ORCID ProfileSeiya Oura, View ORCID ProfileTakayuki Koyano, Sumire Kobayashi, Martin M. Matzuk, and View ORCID ProfileMasahito Ikawa
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 117 Issue: 21 Pages: 11493-11502
  • DOI: 10.1073/pnas.1922650117
  • NAID: 120006957465
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spermatozoa lacking Fertilization Influencing Membrane Protein (FIMP) fail to fuse with oocytes in mice (2020)
  • Author(s): Fujihara Yoshitaka、Lu Yonggang、Noda Taichi、Oji Asami、Larasati Tamara、Kojima-Kita Kanako、Yu Zhifeng、Matzuk Ryan M.、Matzuk Martin M.、Ikawa Masahito
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 117 Issue: 17 Pages: 9393-9400
  • DOI: 10.1073/pnas.1917060117
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Testis‐enriched kinesin KIF9 is important for progressive motility in mouse spermatozoa (2020)
  • Author(s): Miyata Haruhiko、Shimada Keisuke、Morohoshi Akane、Oura Seiya、Matsumura Takafumi、Xu Zoulan、Oyama Yuki、Ikawa Masahito
  • Journal Title: The FASEB Journal Volume: 34 Issue: 4 Pages: 5389-5400
  • DOI: 10.1096/fj.201902755r
  • Peer Reviewed / Open Access
• [Journal Article] TESMIN, METALLOTHIONEIN-LIKE 5, is required for spermatogenesis in mice. (2020)
  • Author(s): Oji A, Isotani A, Fujihara Y, Castaneda JM, Oura S, Ikawa M.
  • Journal Title: Biology of Reproduction Volume: 102 Issue: 4 Pages: 975-983
  • DOI: 10.1093/biolre/ioaa002
  • Peer Reviewed / Open Access
• [Journal Article] Analysis of the sperm flagellar axoneme using gene-modified mice (2020)
  • Author(s): Miyata H, Morohoshi A, Ikawa M.
  • Journal Title: Experimental Animals Volume: 69 Issue: 4 Pages: 374-381
  • DOI: 10.1538/expanim.20-0064
  • NAID: 130007938660
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] CRISPR/Cas9-Mediated Genome Editing Reveals Oosp Family Genes are Dispensable for Female Fertility in Mice (2020)
  • Author(s): Abbasi Ferheen、Kodani Mayo、Emori Chihiro、Kiyozumi Daiji、Mori Masashi、Fujihara Yoshitaka、Ikawa Masahito
  • Journal Title: Cells Volume: 9 Issue: 4 Pages: 821-821
  • DOI: 10.3390/cells9040821
  • Peer Reviewed / Open Access
• [Journal Article] Nexin-Dynein regulatory complex component DRC7 but not FBXL13 is required for sperm flagellum formation and male fertility in mice (2020)
  • Author(s): Morohoshi Akane、Miyata Haruhiko、Shimada Keisuke、Nozawa Kaori、Matsumura Takafumi、Yanase Ryuji、Shiba Kogiku、Inaba Kazuo、Ikawa Masahito
  • Journal Title: PLOS Genetics Volume: 16 Issue: 1 Pages: 1-21
  • DOI: 10.1371/journal.pgen.1008585
  • NAID: 120007159540
  • Peer Reviewed / Open Access
• [Journal Article] Genetic mutation of <i>Frem3</i> does not causeFraser syndrome in mice (2020)
  • Author(s): Kiyozumi Daiji、Mori Masashi、Kodani Mayo、Ikawa Masahito
  • Journal Title: Experimental Animals Volume: 69 Issue: 1 Pages: 104-109
  • DOI: 10.1538/expanim.19-0088
  • NAID: 130007791541
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] Mouse t-complex protein 11 is important for progressive motility in sperm† (2019)
  • Author(s): Castaneda Julio M、Miyata Haruhiko、Archambeault Denise R、Satouh Yuhkoh、Yu Zhifeng、Ikawa Masahito、Matzuk Martin M
  • Journal Title: Biology of Reproduction Volume: 102 Issue: 4 Pages: 852-862
  • DOI: 10.1093/biolre/ioz226
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Male mice, caged in the International space station for 35 days, sire healthy offspring. (2019)
  • Author(s): Matsumura T, Noda T, Muratani M, Okada R, Yamane M, Isotani A, Kudo T, Takahashi S, Ikawa M.
  • Journal Title: Scientific Research Volume: 9 Issue: 1 Pages: 13733-13733
  • DOI: 10.1038/s41598-019-50128-w
  • NAID: 120006957452
  • Peer Reviewed / Open Access
• [Journal Article] Identification of multiple male reproductive tract-specific proteins that regulate sperm migration through the oviduct in mice (2019)
  • Author(s): Fujihara Yoshitaka、Noda Taichi、Kobayashi Kiyonori、Oji Asami、Kobayashi Sumire、Matsumura Takafumi、Larasati Tamara、Oura Seiya、Kojima-Kita Kanako、Yu Zhifeng、Matzuk Martin M.、Ikawa Masahito
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 116 Issue: 37 Pages: 18498-18506
  • DOI: 10.1073/pnas.1908736116
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice† (2019)
  • Author(s): Yonggang Lu, Oura Seiya, Matsumura Takafumi, et al.
  • Journal Title: Biology of Reproduction Volume: 101 Issue: 2 Pages: 501-511
  • DOI: 10.1093/biolre/ioz103
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Nine genes abundantly expressed in the epididymis are not essential for male fecundity in mice (2019)
  • Author(s): Noda T、Sakurai N、Nozawa K、Kobayashi S、Devlin D J、Matzuk M M、Ikawa M
  • Journal Title: Andrology Volume: 7 Issue: 5 Pages: 644-653
  • DOI: 10.1111/andr.12621
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] <i>Lvrn</i> expression is not critical for mouse placentation (2019)
  • Author(s): Tobita T, Kiyozumi D, Muto M, Noda T, Ikawa M.
  • Journal Title: Journal of Reproduction and Development Volume: 65 Issue: 3 Pages: 239-244
  • DOI: 10.1262/jrd.2018-157
  • NAID: 130007663049
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis (2019)
  • Author(s): Shimada K, Kato H, Miyata H, Ikawa M.
  • Journal Title: Journal of Reproduction and Development Volume: 65 Issue: 2 Pages: 155-162
  • DOI: 10.1262/jrd.2018-136
  • NAID: 130007630929
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] Seminal vesicle secretory protein 7, PATE4, is not required for sperm function but for copulatory plug formation to ensure fecundity. (2019)
  • Author(s): Noda T, Fujihara Y, Matsumura T, Oura S, Kobayashi S, Ikawa M.
  • Journal Title: Biology of reproduction Volume: 印刷中 Issue: 4 Pages: 1035-1045
  • DOI: 10.1093/biolre/ioy247
  • Peer Reviewed / Open Access
• [Journal Article] PTBP1 contributes to spermatogenesis through regulation of proliferation in spermatogonia (2019)
  • Author(s): SENOO Manami、TAKIJIRI Takashi、YOSHIDA Nobuaki、OZAWA Manabu、IKAWA Masahito
  • Journal Title: Journal of Reproduction and Development Volume: 65 Issue: 1 Pages: 37-46
  • DOI: 10.1262/jrd.2018-109
  • NAID: 130007594357
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] Chimeric analysis with newly established EGFP/DsRed2-tagged ES cells identify HYDIN as essential for spermiogenesis in mice (2019)
  • Author(s): Oura Seiya、Miyata Haruhiko、Noda Taichi、Shimada Keisuke、Matsumura Takafumi、Morohoshi Akane、Isotani Ayako、Ikawa Masahito
  • Journal Title: Experimental Animals Volume: 68 Issue: 1 Pages: 25-34
  • DOI: 10.1538/expanim.18-0071
  • NAID: 130007604401
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] Infection with flaviviruses requires BCLXL for cell survival. (2018)
  • Author(s): Suzuki T, Okamoto T, Katoh H, Sugiyama Y, Kusakabe S, Tokunaga M, Hirano J, Miyata Y, Fukuhara T, Ikawa M, Satoh T, Yoshio S, Suzuki R, Saijo M, Huang DCS, Kanto T, Akira S Matsuura Y
  • Journal Title: PLoS Pathog Volume: 14 Issue: 9 Pages: e1007299-e1007299
  • DOI: 10.1371/journal.ppat.1007299
  • NAID: 120006957426
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Co-expression of sperm membrane proteins CMTM2A and CMTM2B is essential for ADAM3 localization and male fertility in mice. (2018)
  • Author(s): Fujihara Y, Oji A, Kojima-Kita K, Larasati T, Ikawa M.
  • Journal Title: Journal of cell science Volume: 131 Pages: 221481-221481
  • DOI: 10.1242/jcs.221481
  • NAID: 130007719285
  • Peer Reviewed / Open Access
• [Journal Article] RSPH6A is required for sperm flagellum formation and male fertility in mice (2018)
  • Author(s): Abbasi Ferheen、Miyata Haruhiko、Shimada Keisuke、Morohoshi Akane、Nozawa Kaori、Matsumura Takafumi、Xu Zoulan、Pratiwi Putri、Ikawa Masahito
  • Journal Title: Journal of Cell Science Volume: 131 Pages: 1-11
  • DOI: 10.1242/jcs.221648
  • NAID: 120006957445
  • Peer Reviewed / Open Access
• [Journal Article] Engineered CRISPR-Cas9 nuclease with expanded targeting space (2018)
  • Author(s): Nishimasu Hiroshi、Shi Xi、Ishiguro Soh、Gao Linyi、Hirano Seiichi、Okazaki Sae、Noda Taichi、Abudayyeh Omar O.、Gootenberg Jonathan S.、Mori Hideto、Oura Seiya、Holmes Benjamin、Tanaka Mamoru、Seki Motoaki、Hirano Hisato、Aburatani Hiroyuki、Ishitani Ryuichiro、Ikawa Masahito、Yachie Nozomu、Zhang Feng、Nureki Osamu
  • Journal Title: Science Volume: 361 Issue: 6408 Pages: 1259-1262
  • DOI: 10.1126/science.aas9129
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Sperm-borne phospholipase C zeta-1 ensures monospermic fertilization in mice (2018)
  • Author(s): Nozawa Kaori、Satouh Yuhkoh、Fujimoto Takao、Oji Asami、Ikawa Masahito
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1315-1315
  • DOI: 10.1038/s41598-018-19497-6
  • NAID: 120006957442
  • Peer Reviewed / Open Access
• [Journal Article] Factors controlling sperm migration through the oviduct revealed by gene-modified mouse models (2018)
  • Author(s): fujihara Y, Miyata H, Ikawa M.
  • Journal Title: Experimental Animals Volume: 67 Issue: 2 Pages: 91-104
  • DOI: 10.1538/expanim.17-0153
  • NAID: 130006733631
  • ISSN: 0007-5124, 1341-1357, 1881-7122
  • Peer Reviewed / Open Access
• [Journal Article] Human Globozoospermia-Related Gene Spata16 Is Required for Sperm Formation Revealed by CRISPR/Cas9-Mediated Mouse Models (2017)
  • Author(s): Fujihara Yoshitaka、Oji Asami、Larasati Tamara、Kojima-Kita Kanako、Ikawa Masahito
  • Journal Title: Int J Mol Sci. Volume: 18 Issue: 10 Pages: 2208-2208
  • DOI: 10.3390/ijms18102208
  • Peer Reviewed / Open Access
• [Journal Article] Mouse spermatozoa with higher fertilization rates have thinner nuclei. (2017)
  • Author(s): Mashiko D, Ikawa M, Fujimoto K.
  • Journal Title: PeerJ Volume: 5 Pages: e3913-e3913
  • DOI: 10.7717/peerj.3913
  • Peer Reviewed / Open Access
• [Presentation] ゲノム編集と受精研究への応用 (2021)
  • Author(s): 伊川正人
  • Organizer: 第17回熊本大学生命資源・研究センターシンポジウム
  • Invited
• [Presentation] CRISPR/Cas9 Mediated Genome Editing and Its Application for the Study of Fertilization in Mice (2020)
  • Author(s): Masahito Ikawa
  • Organizer: The University of Kansas Medical Center seminar
  • Int'l Joint Research / Invited
• [Presentation] ゲノム編集マウスが切り開く受精研究の最前線 (2020)
  • Author(s): 伊川正人
  • Organizer: 第38回日本受精着床学会総会・学術講演会
  • Invited
• [Presentation] 精子カルシニューリン（PPP3cc/PPP3r2）と精子受精能力 (2020)
  • Author(s): 伊川正人
  • Organizer: 第93回日本生化学会大会シンポジウム
  • Invited
• [Presentation] 最新の方法と将来の展望 ①ゲノム編集技術と遺伝子改変動物の作成 (2020)
  • Author(s): 伊川正人
  • Organizer: 第84回日本循環器学会学術集会
  • Invited
• [Presentation] ゲノム編集を利用した遺伝子治療および最先端ゲノム編集技術とその安全性 (2020)
  • Author(s): 伊川正人
  • Organizer: 第47回日本毒性学会学術年会
  • Invited
• [Presentation] CRISPR/Cas9 Mediated Genome Editing and Its Application for the Study of Fertilization (2019)
  • Author(s): Masahito Ikawa
  • Organizer: Gordon Research Conference (Germ Cell Programming in Vertebrate Biology, Medicine and Biotechnology)
  • Int'l Joint Research / Invited
• [Presentation] CRISPR/Cas9 Mediated Genome Editing in Mice (2019)
  • Author(s): 伊川正人
  • Organizer: The 15th Transgenic Technology Meeting
  • Invited
• [Presentation] ゲノム編集による遺伝子改変マウス作製と生殖医学研究への応用 (2019)
  • Author(s): 伊川正人
  • Organizer: 第30回日本医学会総会 2019
  • Invited
• [Presentation] ゲノム編集と生殖医学研究 (2019)
  • Author(s): 伊川正人
  • Organizer: 第18回生殖バイオロジー東京シンポジウム
  • Invited
• [Presentation] CRISPR/Cas9 mediated genome editing and its application for the study of reproduction (2019)
  • Author(s): Masahito Ikawa
  • Organizer: CEBST2019
  • Int'l Joint Research / Invited
• [Presentation] New Insights into the Molecular Events of Mammalian Fertilization (2019)
  • Author(s): Ikawa M
  • Organizer: Fifth International Oocyte Meeting
  • Int'l Joint Research / Invited
• [Presentation] CRISPR/Cas9 Mediated Genome Editing in Mice and Its Application for the Study of Reproduction (2018)
  • Author(s): Ikawa M
  • Organizer: Yanagimachi 90th Birthday Symposium
  • Int'l Joint Research / Invited
• [Presentation] CRISPR/Cas9 Mediated Genome Editing in Mice and Its Application for the Study of Reproduction (2018)
  • Author(s): Ikawa M
  • Organizer: 7th International Conference on the Epididymis
  • Int'l Joint Research / Invited
• [Presentation] CRISPR/Cas9 mediated genome editing in mice and its application for the study of reproduction (2018)
  • Author(s): 伊川 正人
  • Organizer: 第9回武田科学振興財団薬科学シンポジウム
  • Invited
• [Presentation] CRISPR/Cas9 mediated genome editing in mice and its application for the study of reproduction (2018)
  • Author(s): 伊川 正人
  • Organizer: Bridging Biomedical Worlds 2018
  • Int'l Joint Research / Invited
• [Presentation] Fertilization and Oocyte Activation: New Insights Using Genome Editing (2017)
  • Author(s): 伊川 正人
  • Organizer: Fertilization & Activation of Development(Gordon Research Conference)
  • Int'l Joint Research / Invited
• [Presentation] CRISPR/Cas9 Mediated Genome Editing and its Application for the Study of Reproduction (2017)
  • Author(s): 伊川 正人
  • Organizer: JST戦略的国際共同研究プログラム
  • Int'l Joint Research / Invited
• [Presentation] CRISPR/Cas9 mediated genome editing and its application for the study of sperm functions (2017)
  • Author(s): 伊川 正人
  • Organizer: 11th International Congress of Andrology
  • Int'l Joint Research / Invited
• [Remarks] 研究室ホームページ
  • URL: https://egr.biken.osaka-u.ac.jp/
• [Remarks] 研究室ホームページ
  • URL: http://www.egr.biken.osaka-u.ac.jp/information/results/index.html