| Budget Amount *help |
¥41,860,000 (Direct Cost: ¥32,200,000、Indirect Cost: ¥9,660,000)
Fiscal Year 2020: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2019: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2018: ¥10,270,000 (Direct Cost: ¥7,900,000、Indirect Cost: ¥2,370,000)
Fiscal Year 2017: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
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| Outline of Final Research Achievements |
To explore the higher order structure of the nucleus, we intended to develop a method to detect any genomic regions within a certain spatial distance from a genomic locus of interest and a method for live-cell imaging of a single-copy genomic locus For the former, we succeeded in methylation of the vicinity of a target site bound by a dCas9 to which Dam methylase is tethered to the gRNA through the MS2-MCP interaction. However, we have failed to obtain evidence for methylation at genomic regions spatially proximal to the dCas9-bound site. For the latter, we improved the method for dCas9-mediated bimolecular fluorescence complementation, which enables live cell imaging on chromatin with high singal-to-noise ratio.
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