| Budget Amount *help |
¥42,900,000 (Direct Cost: ¥33,000,000、Indirect Cost: ¥9,900,000)
Fiscal Year 2019: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
Fiscal Year 2018: ¥14,560,000 (Direct Cost: ¥11,200,000、Indirect Cost: ¥3,360,000)
Fiscal Year 2017: ¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
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| Outline of Final Research Achievements |
By endogenous circadian clock mechanisms, living organisms on Earth can measure 24 h of a day to enhance fitness on the day/night alternating environment. Gene expression of clock genes has been assumed as the time measuring process. However, three Kai proteins of cyanobacteria can precisely measure 24 h in the test tube. This study aimed to elucidate the time measuring mechanism in KaiC protein. We confirmed that KaiC generate a tension in KaiC structure by negative feedback of energy released from hydrolysis of very tiny portion of ATP. Our data on the ATPase activity of KaiC support our hypothesis that protein clock is designed in a similar fashion of mechanical clock.
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