Elucidating the mechanisms of spindle formation through systematic gene deletion

• Project/Area Number: 17H01431
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cell biology
• Research Institution: Nagoya University
• Principal Investigator: Goshima Gohta 名古屋大学, 理学研究科, 教授 (20447840)
• Co-Investigator(Kenkyū-buntansha): 清光 智美 沖縄科学技術大学院大学, 細胞分裂動態ユニット, 准教授 (10503443)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Project Status: Completed (Fiscal Year 2022)
• Budget Amount: ¥41,990,000 (Direct Cost: ¥32,300,000、Indirect Cost: ¥9,690,000); Fiscal Year 2021: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000); Fiscal Year 2020: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000); Fiscal Year 2019: ¥8,450,000 (Direct Cost: ¥6,500,000、Indirect Cost: ¥1,950,000); Fiscal Year 2018: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000); Fiscal Year 2017: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
• Keywords: 細胞

Outline of Final Research Achievements

The spindle is essential for chromosome segregation. As many genes act redundantly, the molecular mechanisms of spindle formation are not fully understood. By systematically disrupting candidate spindle regulators in model cell systems, we studied the mechanism of spindle parts formation, including the kinetochore, which is the contact point between chromosome and spindle, the ends of microtubules, which are the main components of the spindle, and the spindle pole.

Academic Significance and Societal Importance of the Research Achievements

細胞分裂装置・スピンドルの形成に必要なタンパク質は、代表的なモデル細胞系を用いて網羅的に明らかにされてきた。本研究では、これらのうちいくつかのタンパク質について分子活性や作用機序を解明するとともに、複数のタンパク質が協調して機能するケースをいくつか示すことができた。細胞分裂時の諸過程の制御に複数の機構が同時に関わりうることを明らかにしたことが、重要な成果のひとつである。

Research Products

• [Int'l Joint Research] The University of Edinburgh(英国)
• [Int'l Joint Research] The University of Edinburgh(英国)
• [Int'l Joint Research] The University of Edinburgh(英国)
• [Int'l Joint Research] The University of Edinburgh(英国)
• [Int'l Joint Research] The University of Edinburgh(英国)
• [Journal Article] Mitotic spindle formation in the absence of Polo kinase (2022)
  • Author(s): Kim Juyoung、Goshima Gohta
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 119 Issue: 12
  • DOI: 10.1073/pnas.2114429119
  • Peer Reviewed
• [Journal Article] Further Reading | Microtubule Nucleation Pathways (2021)
  • Author(s): Goshima Gohta
  • Journal Title: Encyclopedia of Biological Chemistry III (Third Edition) Volume: 5 Pages: 547-553
  • DOI: 10.1016/b978-0-12-819460-7.00069-4
  • ISBN: 9780128220405
  • Peer Reviewed
• [Journal Article] Microtubule-associated proteins promote microtubule generation in the absence of γ-tubulin in human colon cancer cells (2021)
  • Author(s): Tsuchiya Kenta、Goshima Gohta
  • Journal Title: Journal of Cell Biology Volume: 220 Issue: 12
  • DOI: 10.1083/jcb.202104114
  • Peer Reviewed
• [Journal Article] Ran-GTP Is Non-essential to Activate NuMA for Mitotic Spindle-Pole Focusing but Dynamically Polarizes HURP Near Chromosomes (2021)
  • Author(s): Tsuchiya Kenta、Hayashi Hisato、Nishina Momoko、Okumura Masako、Sato Yoshikatsu、Kanemaki Masato T.、Goshima Gohta、Kiyomitsu Tomomi
  • Journal Title: Current Biology Volume: 31 Issue: 1 Pages: 115-127.e3
  • DOI: 10.1016/j.cub.2020.09.091
  • Peer Reviewed / Open Access
• [Journal Article] Kinetochore protein depletion underlies cytokinesis failure and somatic polyploidization in the moss Physcomitrella patens (2019)
  • Author(s): Kozgunova Elena、Nishina Momoko、Goshima Gohta
  • Journal Title: eLife Volume: 8
  • DOI: 10.7554/elife.43652
  • Peer Reviewed / Open Access
• [Journal Article] Editorial overview: Cell division - from molecules to tissues (2019)
  • Author(s): Goshima Gohta、Bellaiche Yohanns
  • Journal Title: Current Opinion in Cell Biology Volume: 60 Pages: iii-v
  • DOI: 10.1016/j.ceb.2019.06.006
  • Int'l Joint Research
• [Journal Article] Drosophila kinesin-8 stabilizes the kinetochore-microtubule interaction (2019)
  • Author(s): Edzuka Tomoya, Goshima Goshima
  • Journal Title: The Journal of cell biology Volume: 218(2) Issue: 2 Pages: 474-488
  • DOI: 10.1083/jcb.201807077
  • Peer Reviewed
• [Journal Article] Importin-β targets HURP to kinetochore-fibers in coordination with Ran-GTP in human mitotic cells (2018)
  • Author(s): Tsuchiya K, Hayashi H, Nishina M, Okumura M, Kanemaki MT, Goshima G, Kiyomitsu T
  • Journal Title: bioRxiv Volume: 1 Pages: 1-31
  • DOI: 10.1101/473538
  • Open Access
• [Journal Article] 14-3-3 regulation of Ncd reveals a new mechanism for targeting proteins to the spindle in oocytes (2017)
  • Author(s): Beaven Robin、Bastos Ricardo Nunes、Spanos Christos、Rom? Pierre、Cullen C. Fiona、Rappsilber Juri、Giet R?gis、Goshima Gohta、Ohkura Hiroyuki
  • Journal Title: The Journal of Cell Biology Volume: 216 Issue: 10 Pages: 3029-3039
  • DOI: 10.1083/jcb.201704120
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2 (2017)
  • Author(s): Tungadi Elsa A.、Ito Ami、Kiyomitsu Tomomi、Goshima Gohta
  • Journal Title: Journal of Cell Science Volume: 130 Issue: 21 Pages: 3676-3684
  • DOI: 10.1242/jcs.203703
  • Peer Reviewed
• [Presentation] 細胞分裂の宝探し (2022)
  • Author(s): 五島剛太
  • Organizer: 細胞分裂研究会
  • Invited
• [Presentation] Cell division without a gene required for cell division (2021)
  • Author(s): 五島剛太
  • Organizer: The Biochemical Society and the British Society for Cell Biology -Dynamic Cell IV-
  • Int'l Joint Research / Invited
• [Presentation] Spindle formation without a gene required for spindle formation (2021)
  • Author(s): 五島剛太
  • Organizer: Mitotic spindle: From living and synthetic systems to theory
  • Int'l Joint Research / Invited
• [Presentation] Consequences of chromosome mis-segregation in moss stem cell (2019)
  • Author(s): 五島剛太
  • Organizer: International Symposium: Principles of pluripotent stem cells underlying plant vitality
  • Int'l Joint Research / Invited
• [Presentation] 多様な生命現象を司る微小管 -中心体集合システム- (2019)
  • Author(s): 五島剛太
  • Organizer: 第92回日本生化学学会大会
  • Invited
• [Presentation] The development of mitosis-specific rapid protein-degradation assays in human cells (2019)
  • Author(s): 清光智美
  • Organizer: EMBO | EMBL Symposium: Seeing is Believing - Imaging the Molecular Processes of Life
  • Int'l Joint Research
• [Presentation] Mitosis in plants (2019)
  • Author(s): 五島剛太
  • Organizer: HBIGS WINTER SCHOOL for PhD students 2019: Molecular mechanisms in mitosis
  • Int'l Joint Research / Invited
• [Presentation] Cell division in moss stem cells (2019)
  • Author(s): 五島剛太
  • Organizer: 第60回日本植物生理学会年会
  • Invited
• [Presentation] Cell division in plants (2018)
  • Author(s): 五島剛太
  • Organizer: 植物研究発表会
  • Int'l Joint Research / Invited
• [Presentation] Microtubules and cell division in plants (2018)
  • Author(s): 五島剛太
  • Organizer: The 7th IMPRS-CMB Students' Symposium
  • Int'l Joint Research / Invited
• [Presentation] Mechanism of cell division in plants (2018)
  • Author(s): 五島剛太
  • Organizer: 第41回日本分子生物学会年会
  • Invited
• [Presentation] Importin-β recruits HURP to k-fibers in cooperation with Ran-GTP to form proper metaphase spindle (2018)
  • Author(s): Kenta Tsuchiya, Hisato Hayashi, Momoko Nishina, Masako Okumura, Masato T. Kanemaki, Gohta Goshima, Tomomi Kiyomitsu
  • Organizer: 第41回日本分子生物学会年会
• [Presentation] Spindle pole organisation by the microcephaly protein ASPM (2017)
  • Author(s): 五島剛太
  • Organizer: 神経細胞シンポジウム「International Symposium on Neural Precursor Cell Fate Determination, Differentiation and Neuronal Circuit Formation」
  • Int'l Joint Research / Invited
• [Presentation] Spindle pole focusing by the microcephaly protein ASPM (2017)
  • Author(s): 五島剛太
  • Organizer: CSH Asia 2017 Conference on Cilia & Centrosomes
  • Int'l Joint Research / Invited
• [Presentation] ショウジョウバエにおいて動原体と微小管の安定な結合にはキネシン8が必要である (2017)
  • Author(s): 江塚智哉, 五島剛太
  • Organizer: 2017年度生命科学系学会合同年次会
• [Remarks] 五島研究室ホームページ
  • URL: http://bunshi4.bio.nagoya-u.ac.jp/~tenure2/goshima.html