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Chemical ecology of sexual isolation and reproductive interference in pulmonates

Research Project

Project/Area Number 17H01448
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Ecology/Environment
Research InstitutionShinshu University

Principal Investigator

ASAMI Takahiro  信州大学, 理学部, 特任教授 (10222598)

Co-Investigator(Kenkyū-buntansha) 吉村 仁  長崎大学, 熱帯医学研究所, 客員教授 (10291957)
朴 虎東  信州大学, 学術研究院理学系, 教授 (20262686)
渡辺 信子  神奈川大学, 理学部, 助教 (40291744)
伊集院 久子  神奈川大学, 付置研究所, 研究員 (60398948)
國頭 恭  信州大学, 学術研究院理学系, 教授 (90304659)
蟻川 謙太郎  総合研究大学院大学, 先導科学研究科, 教授 (20167232)
Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥44,590,000 (Direct Cost: ¥34,300,000、Indirect Cost: ¥10,290,000)
Fiscal Year 2021: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2020: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2019: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2018: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥33,930,000 (Direct Cost: ¥26,100,000、Indirect Cost: ¥7,830,000)
Keywords有肺類 / 性フェロモン / mate recognition
Outline of Final Research Achievements

Stylommatophorans, which are simultaneous reciprocal hermaphrodites, court and mate after approaching each other depending on chemical recognition of volatile excretes from conspecifics. Interspecific F1 mature hybrids attract adults of parental species. We achieved technical renovations of the original bioassey device and experimental protocols to examine behavioral responses to volatiles. These hermaphrodites in proximity excrete attracting compounds and also locates each other by sensing the compounds from the others. Based on this characteristic we developed a simple method to condition the subject mature animals to be ready for those interactions with other adults. We made hypothetically presumed air disturbance where two airflows meet inside the alfactometer and developed a method to manipulate air flow parameters to resolve the problem of volatile leaks back to the control air route.

Academic Significance and Societal Importance of the Research Achievements

本研究により、累代飼育法に独自の改良を重ねたコハクオナジマイマイとオナジマイマイを、生殖的隔離機構の生態・行動・遺伝解析が可能な雌雄同体動物をのモデル動物として確立した。柄眼類(カタツムリ・ナメクジ)の性フェロモンの化学特性・生態機能に関する知見は皆無である。本2種の利点を生かし、前例のないオルファクトメータを実用化し、性フェロモンによる配偶者識別の生物検定法を確立した。性フェロモンの産生と応答にみる種間雑種個体の特徴を明らかにした。以上により、性フェロモンを介しての交配前の生殖隔離種機構を理解するためのさらなる進化生物学的および化学生態学的な研究を進める基盤を固めた。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (11 results)

All 2022 2021

All Journal Article (11 results) (of which Peer Reviewed: 11 results)

  • [Journal Article] Dissolved organic nitrogen, dinoflagellates and cyanobacteria in two eutrophic lakes: Analysis by inferential modelling2022

    • Author(s)
      Friedrich Recknagel, Ho-Dong Park, Assaf Sukenik and Tamar Zohary
    • Journal Title

      Harmful Algae

      Volume: 114 Pages: 1-11

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Origin of Deep Methane from Active Faults along the Itoigawa-Shizuoka Tectonic Line between the Eurasian and North American Plates: 13C/12C & 14C/12C Methane Profiles from a Pull-Apart Basin at Lake Suwa2022

    • Author(s)
      Urai Atsushi, Yoshinori Takano, Yohei Matsu, Hiroki Iwata, Yosuke Miyairi, Yusuke Yokoyama, Yuichi Miyabara, Naohiko Ohkouchi, and Ho-Dong Park
    • Journal Title

      ACS Earth and Space Chemistry

      Volume: 6 Pages: 1-11

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two subspecies of the snail mite Riccardoella (Proriccardoella) reaumuri Fain & van Goethem, 1986 (Acari, Prostigmata, Ereynetidae) from Japan2022

    • Author(s)
      Waki Tsukasa、Motochin Risho、Asami Takahiro、Shimano Satoshi
    • Journal Title

      Systematic and Applied Acarology

      Volume: - Pages: 839-854

    • DOI

      10.11158/saa.27.5.2

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] pH is the dominant factor controlling the levels of phytate-like and DNA-like phosphorus in 0.5M NaHCO3-extracts of soils: evaluation with phosphatase-addition approach.2021

    • Author(s)
      Kunito, T., Haraguchi, S., Hanada, K., Fujita, K., Moro, H., Nagaoka, N., and Otsuka, S
    • Journal Title

      Geoderma

      Volume: 398 Pages: 115113-115113

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Organic phosphorus substantially contributes to crop plant nutrition in soils with low phosphorus availability2021

    • Author(s)
      Moro, H., Park, H.-D., and Kunito, T.
    • Journal Title

      Agronomy

      Volume: 11 Pages: 903-903

    • NAID

      120007113362

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of planktonic coenzyme factor 430 in a freshwater lake: small-scale analysis for probing archaeal methanogenesis2021

    • Author(s)
      Urai Atsushi, Makoto Matsushita, Ho Dong Park, Hiroyuki Imachi, Miyuki Ogawara, Hiroki Iwata, Masanori Kaneko, Nanako O. Ogawa, Naohiko Ohkouchi and Yoshinori Takano
    • Journal Title

      Prog Earth Planet Sci

      Volume: 8 Pages: 1-17

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Origin of Deep Methane Associated with a Unique Community of Microorganisms in an Organic- and Iodine-Rich Aquifer2021

    • Author(s)
      Urai Atsushi, Yoshinori Takano, Hiroyuki Imachi, Shun’ichi Ishii, Yohei Matsui, Miyuki Ogawara, Eiji Tasumi, Yosuke Miyairi, Nanako O. Ogawa, Toshihiro Yoshimura, Fumio Inagaki, Yusuke Yokoyama, Kenjiro Kawano, Daisuke Murai, Ho-Dong Park, and Naohiko Ohkouchi
    • Journal Title

      ACS Earth and Space Chemistry

      Volume: 5 Pages: 1-11

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Solvent effect on base-induced chemiluminescent decomposition of bicyclic dioxetanes bearing a 3-hydroxyphenyl group2021

    • Author(s)
      Watanabe, Nobuko; Ijuin, Hisako K.; Takatsuka, Hikaru; Matsumoto, Masakatsu
    • Journal Title

      Tetrahedron

      Volume: 88 Pages: 132147-132147

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Two-step mechanism of spiral phyllotaxis2021

    • Author(s)
      T Okabe, J Yoshimura.
    • Journal Title

      Journal of Theoretical Biology

      Volume: 508 Pages: 110484-110484

    • DOI

      10.1016/j.jtbi.2020.110484

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Eight-year periodical outbreaks of the train millipede2021

    • Author(s)
      Niijima Keiko、Nii Momoka、Yoshimura Jin
    • Journal Title

      Royal Society Open Science

      Volume: 8 Issue: 1 Pages: 201399-201399

    • DOI

      10.1098/rsos.201399

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Systematics and convergent evolution in three Australian genera of Pepsinae spider wasps (Hymenoptera: Pompilidae)2021

    • Author(s)
      Shimizu Akira、Pitts James P、Rodriguez Juanita、Wahis Raymond、Yoshimura Jin
    • Journal Title

      Austral Entomology

      Volume: 60 Issue: 2 Pages: 301-316

    • DOI

      10.1111/aen.12530

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed

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Published: 2017-04-28   Modified: 2023-01-30  

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