Characterization of quantitative trait loci regulated by environmental condition.

• Project/Area Number: 17H01458
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Science in genetics and breeding
• Research Institution: Nagoya University
• Principal Investigator: Matsuoka Makoto 名古屋大学, 生物機能開発利用研究センター, 教授 (00270992)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥42,770,000 (Direct Cost: ¥32,900,000、Indirect Cost: ¥9,870,000); Fiscal Year 2019: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000); Fiscal Year 2018: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000); Fiscal Year 2017: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
• Keywords: 作物育種 / 遺伝子 / 出穂期 / 開花制御 / 窒素施肥 / 育種学 / 遺伝学 / 植物

Outline of Final Research Achievements

The purpose of this research was isolation and study of gene(s), which prolongs the heading time of rice under high nitrogen (N) content. By a genome wide association study (GWAS), we identified HD6 which delays the heading time through the collaborations of HD1 and HD2. The mRNA of HD6 was not changed by the N level but its protein was increased by its increased stability, and the increased HD6 enhanced the HD2 protein level. HD2 suppresses the induction activity of HD1 to HD3a (florigen) expression, whereas HD6 is involved in this regulation through regulating the HD2 by dose-dependent manner.

Academic Significance and Societal Importance of the Research Achievements

本研究の目的は、環境に呼応して機能を変化させる農業関連遺伝子の単離・解析手法の確立とそれを利用した分子育種であった。農業に関連する多くの形質は量的形質であり、その研究は従来QTL解析により研究が進められているが、本手法は多大な時間と労力を要すため代替の手法が強く求められている。さらに重要農業形質は気象や土壌などの外的環境によりダイナミックに影響を受け環境条件を加味した遺伝解析が強く望まれている。そこで本研究はQTL解析ではなくゲノムワイド関連(GWA)解析を用いることで、外的環境に呼応してその機能を変化させるQTL遺伝子の単離・解析技術を構築し、分子育種に貢献することを目指した。

Research Products

• [Journal Article] Genome‐wide expression quantitative trait loci studies facilitate isolation of causal genes controlling panicle structure (2020)
  • Author(s): Wang Fanmiao、Yano Kenji、Nagamatsu Shiro、Inari‐Ikeda Mayuko、Koketsu Eriko、Hirano Ko、Aya Koichiro、Matsuoka Makoto
  • Journal Title: The Plant Journal Volume: ahead of print Issue: 1 Pages: 266-278
  • DOI: 10.1111/tpj.14726
  • Peer Reviewed / Open Access
• [Journal Article] GWAS with principal component analysis identifies a gene comprehensively controlling rice architecture (2019)
  • Author(s): Yano Kenji、Morinaka Yoichi、Wang Fanmiao、Huang Peng、Takehara Sayaka、Hirai Takaaki、Ito Aya、Koketsu Eriko、Kawamura Mayuko、Kotake Kunihiko、Yoshida Shinya、Endo Masaki、Tamiya Gen、Kitano Hidemi、Ueguchi-Tanaka Miyako、Hirano Ko、Matsuoka Makoto
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 116 Issue: 42 Pages: 21262-21267
  • DOI: 10.1073/pnas.1904964116
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Evolution and diversification of the plant gibberellin receptor GID1 (2018)
  • Author(s): Yoshida Hideki、Tanimoto Eiichi、Hirai Takaaki、Miyanoiri Yohei、Mitani Rie、Kawamura Mayuko、Takeda Mitsuhiro、Takehara Sayaka、Hirano Ko、Kainosho Masatsune、Akagi Takashi、Matsuoka Makoto、Ueguchi-Tanaka Miyako
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 115 Issue: 33
  • DOI: 10.1073/pnas.1806040115
  • Peer Reviewed / Open Access
• [Journal Article] Improved nutrient use gives cereal crops a boost (2018)
  • Author(s): Wang Fanmiao、Matsuoka Makoto
  • Journal Title: Nature Volume: 560 Issue: 7720 Pages: 563-564
  • DOI: 10.1038/d41586-018-05928-x
  • Peer Reviewed / Open Access
• [Journal Article] OsIDD2, a zinc finger and INDETERMINATE DOMAIN protein, regulates secondary cell wall formation. (2018)
  • Author(s): Huang, P., Yoshida, H., Yano, K., Kinoshita, S., Kawai, K., Koketsu, E., Hattori, M., Takehara, S., Huang, J., Hirano, K., Ordonio, RL.＊＊, Matsuoka, M., Ueguchi-Tanaka, M.
  • Journal Title: J Integr Plant Biol. Volume: 60 Issue: 2 Pages: 130-143
  • DOI: 10.1111/jipb.12557
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Genome-wide association study using whole-genome sequencing rapidly identifies new genes regulating agronomic traits in rice. (2019)
  • Author(s): Matsuoka, M.
  • Organizer: 2019全基因體關聯性分析及譜系圖譜分析-以自然種原探勘有用基因應用於作物育種研討會
  • Invited
• [Presentation] GWAS with principal component analysis (PCA) reveals a novel gene comprehensively controlling rice architecture. (2019)
  • Author(s): Matsuoka, M.
  • Organizer: Workshop of Rice Functional Genome and Breeding by Molecular Design
  • Invited
• [Presentation] GWAS with PCA for isolating novel genes comprehensively controlling rice architecture. (2019)
  • Author(s): Matsuoka, M.
  • Organizer: Rice Breeding Workshop
  • Invited
• [Presentation] 主成分分析を用いたイネ草型に関するGWA解析. (2019)
  • Author(s): 矢野憲司、平野恒、吉田晋弥、北野英己、田宮元、松岡信
  • Organizer: 日本育種学会第135回講演会
• [Presentation] 米の食味に関するGWA解析. (2019)
  • Author(s): 森政樹、平野恒、川村真結子、小川ひかり、松岡信
  • Organizer: 日本育種学会第135回講演会
• [Presentation] 文献情報を用いたGWA解析. (2018)
  • Author(s): 塩田将平、平野恒、矢野憲司、小竹敬久、吉田晋弥、北野英己、松岡信
  • Organizer: 日本育種学会第133回講演会
• [Presentation] イネGWASで検出されたシグナルの検証には反復試験が重要. (2018)
  • Author(s): 矢野憲司、平野恒、北野英己、田宮元、松岡信
  • Organizer: 日本育種学会第133回講演会