• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Biofunctional design of nanocellulose by physicochemical biomimetics of extracellular matrix

Research Project

Project/Area Number 17H01482
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Wood science
Research InstitutionKyushu University

Principal Investigator

KITAOKA Takuya  九州大学, 農学研究院, 教授 (90304766)

Co-Investigator(Kenkyū-buntansha) 一瀬 博文  九州大学, 農学研究院, 准教授 (00432948)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥43,030,000 (Direct Cost: ¥33,100,000、Indirect Cost: ¥9,930,000)
Fiscal Year 2019: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2018: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2017: ¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Keywordsセルロース / キチン・キトサン / ナノファイバー / 構造多糖 / 界面修飾 / 細胞・組織培養 / バイオインターフェース / バイオマテリアル / 糖鎖 / 細胞・組織 / シグナル伝達 / 生体材料 / ナノ材料 / 細胞培養 / ナノマテリアル
Outline of Final Research Achievements

A novel concept in cell culture engineering has been investigated through physicochemical biomimetics of extracellular matrix found in vivo using natural polysaccharide nanofibers. TEMPO oxidation of bio-inert cellulose provided surface-carboxylated nanocellulose having collagen-like nanofiber form and hyaluronan-like repeated carboxylates, which remarkably enhanced the cell attachment and proliferation of mouse fibroblasts, strongly depending on the carboxy contents and crystalline structures. Besides, the combination with chitosan nanofibers without any cell adhesion capability was surprisingly allowed to boost up the cell proliferation efficiency, far superior to commercial tissue culture polystyrene plates. Pure and structurally-regulated polysaccharide nanofibers, different from extracted bio-components that often cause therapeutic problems, are expected to have the potential to design bioadaptive cell culture scaffolds in “Glyconanoarchitectonics” to realize materials therapy.

Academic Significance and Societal Importance of the Research Achievements

再生医療の発展が切り拓く健康・長寿社会への期待から、iPS細胞をはじめとする細胞側の制御研究に加えて、生体外で細胞・組織培養するための足場材料開発の重要性も高まっている。本研究は、天然多糖の樹木セルロースナノファイバーや甲殻類由来キチンナノファイバーが、人工合成不可能な固有のナノ形状と糖鎖界面を備えることに着目し、既存のプラスチック培養基材では見られない、特異な細胞応答挙動を見出した。特に、これまで細胞接着に不利と考えられてきた親水性基材による細胞接着・増殖の促進効果は、天然多糖の未知機能としての学術的意義に加えて、再生医療用細胞培養基材の実用化の面からも興味深い重要な研究成果である。

Report

(4 results)
  • 2020 Final Research Report ( PDF )
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (44 results)

All 2021 2020 2019 2018 2017 Other

All Journal Article (9 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (32 results) (of which Int'l Joint Research: 9 results,  Invited: 12 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Nanocellulose: Beyond the Ordinary2021

    • Author(s)
      Kojiro Uetani, Takuya Kitaoka
    • Journal Title

      BioResources

      Volume: 16 Pages: 1-4

    • Related Report
      2019 Annual Research Report
    • Open Access
  • [Journal Article] Inherently Distinctive Potentialities and Uses of Nanocellulose Based on its Nanoarchitecture2021

    • Author(s)
      Kojiro Uetani, Naliharifetra Jessica Ranaivoarimanana, Mayumi Hatakeyama, Takuya Kitaoka
    • Journal Title

      BioResources

      Volume: 16

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 糖鎖薄膜の界面設計と細胞接着2020

    • Author(s)
      畠山真由美、北岡卓也
    • Journal Title

      日本接着学会誌

      Volume: 56 Pages: 57-62

    • NAID

      130008094785

    • Related Report
      2019 Annual Research Report
  • [Journal Article] One-step synthesis of cellooligomer-conjugated gold nanoparticles in a water-in-oil emulsion system and their application in biological sensing2019

    • Author(s)
      Mayumi Hatakeyama, Daisuke Ryuno, Shingo Yokota, Hirofumi Ichinose, Takuya Kitaoka
    • Journal Title

      Colloids and Surfaces B: Biointerfaces

      Volume: 178 Pages: 74-79

    • DOI

      10.1016/j.colsurfb.2019.02.051

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Nanocellulose from oil palm biomass to enhance microbial fermentation of butanol for bioenergy applications2019

    • Author(s)
      Novitri Hastuti, Rizki Fitria Darmayanti, Safrina Dyah Hardiningtyas, Kyohei Kanomata, Kenji Sonomoto, Masahiro Goto, Takuya Kitaoka
    • Journal Title

      BioResources

      Volume: 14 Pages: 6936-6957

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Direct stimulation of cellular immune response via TLR2 signaling triggered by contact with hybrid glyco-biointerfaces composed of chitohexaose and cellohexaose2019

    • Author(s)
      Hatakeyama Mayumi, Nakada Fumi, Ichinose Hirofumi, Kitaoka Takuya
    • Journal Title

      Colloids and Surfaces B: Biointerfaces

      Volume: 175 Pages: 517-522

    • DOI

      10.1016/j.colsurfb.2018.12.039

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Insight into metabolic diversity of the brown-rot basidiomycete Postia placenta responsible for sesquiterpene biosynthesis: semi-comprehensive screening of cytochrome P450 monooxygenase involved in protoilludene metabolism2018

    • Author(s)
      Ichinose Hirofumi, Kitaoka Takuya
    • Journal Title

      Microbial Biotechnology

      Volume: 11 Issue: 5 Pages: 952-965

    • DOI

      10.1111/1751-7915.13304

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impacts of amino acid substitutions in fungal cytochrome P450 monooxygenase (CYP57B3) on the effective production of 3′-hydroxygenistein2017

    • Author(s)
      Mayumi Hatakeyama, Takuya Kitaoka, Hirofumi Ichinose
    • Journal Title

      FEMS Microbiology Letters

      Volume: 364 Issue: 11

    • DOI

      10.1093/femsle/fnx107

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Materials Design Inspired by Paper and Cellulose Architectures2017

    • Author(s)
      北岡卓也
    • Journal Title

      Sen'i Gakkaishi

      Volume: 73 Issue: 5 Pages: P-195-P-196

    • DOI

      10.2115/fiber.73.P-195

    • NAID

      130006865311

    • ISSN
      0037-9875, 1884-2259
    • Related Report
      2017 Annual Research Report
    • Open Access
  • [Presentation] 森と海の構造多糖ナノファイバー基板における線維芽細胞の増殖挙動2021

    • Author(s)
      野田朋佳、畠山真由美、北岡卓也
    • Organizer
      第71回日本木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 表面カルボキシ化ナノセルロース薄膜を用いた線維芽細胞の接着および増殖挙動2020

    • Author(s)
      畠山真由美、北岡卓也
    • Organizer
      第70回日本木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] TEMPO酸化セルロースナノファイバー/キトサンナノファイバー混合基板における線維芽細胞の接着および増殖挙動2020

    • Author(s)
      野田朋佳、畠山真由美、北岡卓也
    • Organizer
      第70回日本木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 表面カルボキシ化セルロースナノファイバー薄膜を用いた線維芽細胞の培養2020

    • Author(s)
      畠山真由美、北岡卓也
    • Organizer
      繊維学会年次大会2020
    • Related Report
      2019 Annual Research Report
  • [Presentation] TEMPO酸化セルロースナノファイバー/キトサンナノファイバー混合基板上での線維芽細胞の増殖挙動2020

    • Author(s)
      野田朋佳、畠山真由美、北岡卓也
    • Organizer
      繊維学会年次大会2020
    • Related Report
      2019 Annual Research Report
  • [Presentation] 樹木ナノセルロースで細胞外マトリックスを模倣した細胞培養基材の開発2020

    • Author(s)
      畠山真由美、北岡卓也
    • Organizer
      第87回紙パルプ研究発表会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 森と海の天然多糖ナノファイバー混合基材における線維芽細胞の接着・増殖挙動2020

    • Author(s)
      野田朋佳、畠山真由美、北岡卓也
    • Organizer
      第87回紙パルプ研究発表会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 天然構造多糖のナノ・バイオマテリアルの未来探訪2020

    • Author(s)
      北岡卓也
    • Organizer
      紙パルプ技術協会木材科学セミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Cellulose and Chitosan Nanofibers for Green Organocatalysis2020

    • Author(s)
      Takuya Kitaoka
    • Organizer
      EPNOE (European Polysaccharide Network of Excellence) Junior Online Seminar 2020
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 天然構造多糖のナノ・バイオマテリアルの新機能開拓2020

    • Author(s)
      北岡卓也
    • Organizer
      異分野技術の融合によるCNF社会実装促進事業、第3期(2020年度)ナノセルロース塾
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Nanofibers-based biomaterials composed of surface-carboxylated nanocellulose for cell culture applications2020

    • Author(s)
      Mayumi Hatakeyama, Tomoka Noda, Takuya Kitaoka
    • Organizer
      11th World Biomaterials Congress Virtual
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Nanofibers-based biomaterials composed of nanocellulose and nanochitosan to promote fibroblast cell adhesion and proliferation2020

    • Author(s)
      Tomoka Noda, Mayumi Hatakeyama, Takuya Kitaoka
    • Organizer
      11th World Biomaterials Congress Virtual
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 糖鎖薄膜の界面機能化と細胞接着2019

    • Author(s)
      北岡卓也
    • Organizer
      日本接着学会4研究会合同シンポジウム「接着の未来」
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Fibroblast cell culture on extracellular matrix-mimetic scaffolds composed of surface-carboxylated nanocellulose2019

    • Author(s)
      Mayumi Hatakeyama, Hirofumi Ichinose, Takuya Kitaoka
    • Organizer
      2019 TAPPI International Conference on Nanotechnology for Renewable Materials
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 細胞外マトリックス模倣ナノセルロース薄膜による細胞培養制御2019

    • Author(s)
      畠山真由美、一瀬博文、北岡卓也
    • Organizer
      第86回紙パルプ研究発表会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 表面カルボキシ化ナノセルロース薄膜による細胞培養制御2019

    • Author(s)
      畠山真由美、一瀬博文、北岡卓也
    • Organizer
      セルロース学会第26回年次大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Nanocellulose from Oil Palm Empty Fruit Bunches Enhanced Extractive Fermentation in Microbial Bio-butanol Production2019

    • Author(s)
      Novitri Hastuti, Rizki Fitria Darmayanti, Safrina Dyah Hardiningtyas, Kyohei Kanomata, Kenji Sonomoto, Masahiro Goto, Takuya Kitaoka
    • Organizer
      International Conference on Adopting the Renewable Bioenergy and Waste Utilization to Support Circular Economy & Sustainable Environment
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Biomaterials Innovation for Forest and Marine Polysaccharides2019

    • Author(s)
      Takuya Kitaoka
    • Organizer
      Academic Seminar at King Mongkut's University of Technology North Bangkok
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Magical nanocellulose: TEMPO-oxidized cellulose nanofibers for catalytic and advanced applications2019

    • Author(s)
      Takuya Kitaoka
    • Organizer
      Cellulose-based Biomaterials Workshop at King Mongkut's University of Technology North Bangkok
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] TEMPO酸化セルロースナノファイバー/キトサンナノファイバー混合基板における動物細胞の接着挙動2019

    • Author(s)
      野田朋佳、畠山真由美、北岡卓也
    • Organizer
      第26回日本木材学会九州支部大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Extracellular matrix-mimetic scaffolds based on surface-carboxylated nanocellulose for fibroblast cell culture2019

    • Author(s)
      Mayumi Hatakeyama, Hirofumi Ichinose, Takuya Kitaoka
    • Organizer
      6th International Polysaccharide Conference of EPNOE (European Polysaccharide Network of Excellence)
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 大きな木の中の小さな繊維 -樹木多糖類のナノ・バイオ材料研究-2019

    • Author(s)
      北岡卓也
    • Organizer
      第7回九州大学大学院農学研究院と足寄町との協力協定にもとづく講演会「バイオマス利用の最前線」
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 細胞外マトリックスを構造模倣した表面カルボキシ化ナノセルロース薄膜による線維芽細胞の培養2019

    • Author(s)
      畠山真由美、一瀬博文、北岡卓也
    • Organizer
      第41回日本バイオマテリアル学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] TEMPO酸化セルロースナノファイバー/キトサンナノファイバー混合基板における線維芽細胞の増殖促進効果2019

    • Author(s)
      野田朋佳、畠山真由美、北岡卓也
    • Organizer
      第41回日本バイオマテリアル学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 構造多糖ナノファイバーでECM機能を惹起する細胞培養基材の開発2019

    • Author(s)
      北岡卓也
    • Organizer
      第3回多糖マテリアル研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Concept-driven trial and error to find out new functions of nanocellulose2019

    • Author(s)
      Takuya Kitaoka
    • Organizer
      Failed Brilliance in Nanocellulose Science and Technology, 257th American Chemical Society National Meeting & Exposition 2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 細胞外マトリックスを模倣した表面カルボキシ化ナノセルロース基板上での線維芽細胞の培養2018

    • Author(s)
      畠山真由美、一瀬博文、北岡卓也
    • Organizer
      第50回日本結合組織学会学術大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 表面カルボキシ化ナノセルロースを用いたECM模倣基板上での線維芽細胞の培養2018

    • Author(s)
      畠山真由美、一瀬博文、北岡卓也
    • Organizer
      第40回日本バイオマテリアル学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 細胞外マトリックスを構造模倣した表面カルボキシ化ナノセルロース基板上での動物細胞の接着挙動2018

    • Author(s)
      畠山真由美、一瀬博文、北岡卓也
    • Organizer
      第68回日本木材学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ナノセルロースが主役の先端マテリアルの機能開拓2017

    • Author(s)
      北岡卓也
    • Organizer
      グリーン科学技術研究所セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 糖鎖薄膜のナノ界面構造が決め手の細胞培養基材の設計戦略2017

    • Author(s)
      北岡卓也
    • Organizer
      平成29年度第47回繊維学会夏季セミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 構造多糖の界面を生体反応の相互作用場とする細胞培養基材の設計戦略2017

    • Author(s)
      北岡卓也
    • Organizer
      第66回高分子討論会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Book] 無機/有機材料の表面処理・改質による生体適合性付与「糖鎖薄膜の界面ナノ構造と細胞応答」2019

    • Author(s)
      畠山真由美、北岡卓也(分担執筆)
    • Total Pages
      315
    • Publisher
      シーエムシー出版
    • ISBN
      9784781314150
    • Related Report
      2019 Annual Research Report
  • [Book] 無機/有機材料の表面処理・改質による生体適合性付与:第6章 糖鎖薄膜の界面ナノ構造と細胞応答2019

    • Author(s)
      畠山真由美、北岡卓也
    • Publisher
      シーエムシー出版
    • Related Report
      2018 Annual Research Report
  • [Remarks] 生物資源化学研究室ホームページ

    • URL

      http://bm.wood.agr.kyushu-u.ac.jp/

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report 2017 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2022-01-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi