| Budget Amount *help |
¥42,900,000 (Direct Cost: ¥33,000,000、Indirect Cost: ¥9,900,000)
Fiscal Year 2019: ¥12,350,000 (Direct Cost: ¥9,500,000、Indirect Cost: ¥2,850,000)
Fiscal Year 2018: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2017: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
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| Outline of Final Research Achievements |
Final goal of this study was to develop a method to preserve genetic resources of bitterlings and sticklebacks by combination of cryopreservation and transplantation of their germ-line stem cells. First, we optimized maturational stage of donor testes and recipient age used for the germ-cell transplantation. Second, we confirmed that production of gametes derived from several endangered Japanese bitterlings were possible by using surrogate Chinese rosy bitterlings. Also, cryopreservation of bitterling germ-line stem cells was successfully established.
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