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An impact of evolution of the intestinal symbioses to the host insects:interdisciplinary approaches with micro-metabolomic and genomic analyses

Research Project

Project/Area Number 17H01510
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Insect science
Research InstitutionUniversity of the Ryukyus

Principal Investigator

Tokuda Gaku  琉球大学, 熱帯生物圏研究センター, 教授 (90322750)

Co-Investigator(Kenkyū-buntansha) 木原 久美子  熊本高等専門学校, 拠点化プロジェクト系地域協働プロジェクトグループ, 准教授 (50622916)
北出 理  茨城大学, 理工学研究科(理学野), 教授 (80302321)
福田 真嗣  慶應義塾大学, 政策・メディア研究科(藤沢), 特任教授 (80435677)
本郷 裕一  東京工業大学, 生命理工学院, 教授 (90392117)
Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2022)
Budget Amount *help
¥36,140,000 (Direct Cost: ¥27,800,000、Indirect Cost: ¥8,340,000)
Fiscal Year 2021: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2020: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
Fiscal Year 2019: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2018: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2017: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
Keywords昆虫科学 / 寄生・共生 / 木質代謝 / 昆虫 / セルラーゼ / キシラナーゼ / 共生微生物組成
Outline of Final Research Achievements

In this study, we examined differences in gut microbiota, gut metabolites, and gut protist metabolites in two populations of wood-feeding cockroaches, Cryptocercus punctulatus and C. kyebangensis, geographically isolated in North America and Asia, respectively. The results showed clear differences in the amount of metabolites in the hindgut between these populations. While the study was interrupted by the COVID pandemic, we examined the relationship between changes in gut microbiota and metabolite levels using local termites. We found that only certain gut microbes were highly dependent on the wood consumed by the termites.

Academic Significance and Societal Importance of the Research Achievements

本研究の学術的意義は、微量代謝物測定とシングルセルゲノムとの情報を統合して、特定の共生微生物が宿主に与える影響を捉えることを狙った点にある。すなわち、本研究では最終代謝産物を定量的にとらえることにより、ゲノム解析において推定される代謝系の機能や代謝フローをより直接的に特徴づけることが可能である。さらに社会的意義として、本研究で期待される微生物代謝系が宿主に与えるインパクトに関する知見は、応用昆虫学のみならず微生物生態学や反芻動物を扱う畜産学などの関連分野にも学術的に大きな波及効果をもたらすことが期待される。

Report

(6 results)
  • 2022 Final Research Report ( PDF )
  • 2021 Annual Research Report
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (42 results)

All 2023 2022 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (7 results) Journal Article (17 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 16 results,  Open Access: 9 results) Presentation (18 results) (of which Int'l Joint Research: 5 results,  Invited: 5 results)

  • [Int'l Joint Research] Max Planck Institute(ドイツ)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] North Carolina State University(米国)

    • Related Report
      2021 Annual Research Report
  • [Int'l Joint Research] North Carolina State University(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] North Carolina State University(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] 江原大学校(韓国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] 江原大学校(韓国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Vanderbilt University(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Molecular phylogeny of Spirotrichonymphea (Parabasalia) with emphasis on Spironympha, Spirotrichonympha, and three new genera Pseudospironympha, Nanospironympha, and Brugerollina2023

    • Author(s)
      Noda Satoko、Kitade Osamu、Jasso‐Selles Daniel E.、Taerum Stephen J.、Takayanagi Miki、Radek Renate、Lo Nathan、Ohkuma Moriya、Gile Gillian H.
    • Journal Title

      Journal of Eukaryotic Microbiology

      Volume: 70 Issue: 3

    • DOI

      10.1111/jeu.12967

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Coevolution of Metabolic Pathways in Blattodea and Their Blattabacterium Endosymbionts, and Comparisons with Other Insect-Bacteria Symbioses2022

    • Author(s)
      Kinjo Yukihiro、Bourguignon Thomas、Hongoh Yuichi、Lo Nathan、Tokuda Gaku、Ohkuma Moriya
    • Journal Title

      Microbiology Spectrum

      Volume: 10 Issue: 5 Pages: 02779-22

    • DOI

      10.1128/spectrum.02779-22

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The functional evolution of termite gut microbiota2022

    • Author(s)
      Arora Jigyasa、Kinjo Yukihiro、Sobotnik Jan、Bucek Ales、Clitheroe Crystal、Stiblik Petr、Roisin Yves、Zifcakova Lucia、Park Yung Chul、Kim Ki Yoon、Sillam-Dusses David、Herve Vincent、Lo Nathan、Tokuda Gaku、Brune Andreas、Bourguignon Thomas
    • Journal Title

      Microbiome

      Volume: 10 Issue: 1 Pages: 78-78

    • DOI

      10.1186/s40168-022-01258-3

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Vicariance and dispersal events inferred from mitochondrial genomes and nuclear genes (18S, 28S) shaped global Cryptocercus distributions2022

    • Author(s)
      Che Yanli、Deng Wenbo、Li Weijun、Zhang Jiawei、Kinjo Yukihiro、Tokuda Gaku、Bourguignon Thomas、Lo Nathan、Wang Zongqing
    • Journal Title

      Molecular Phylogenetics and Evolution

      Volume: 166 Pages: 107318-107318

    • DOI

      10.1016/j.ympev.2021.107318

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Genomic and transcriptomic analyses of the subterranean termite <i>Reticulitermes speratus</i> : Gene duplication facilitates social evolution2022

    • Author(s)
      Shigenobu Shuji、Hayashi Yoshinobu、Watanabe Dai、Tokuda Gaku、Hojo Masaru Y. et al.
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 119 Issue: 3 Pages: 1-12

    • DOI

      10.1073/pnas.2110361119

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Fine‐scale genetic diversity and putative ecotypes of oxymonad protists coinhabiting the hindgut of Reticulitermes speratus2021

    • Author(s)
      Igai Katsura、Kitade Osamu、Fu Jieyang、Omata Kazumi、Yonezawa Takahiro、Ohkuma Moriya、Hongoh Yuichi
    • Journal Title

      Molecular Ecology

      Volume: 31 Issue: 4 Pages: 1317-1331

    • DOI

      10.1111/mec.16309

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Enhanced Mutation Rate, Relaxed Selection, and the “Domino Effect” are associated with Gene Loss in Blattabacterium, A Cockroach Endosymbiont2021

    • Author(s)
      Kinjo Yukihiro、Lo Nathan、Martin Paula Villa、Tokuda Gaku、Pigolotti Simone、Bourguignon Thomas
    • Journal Title

      Molecular Biology and Evolution

      Volume: 38 Issue: 9 Pages: 3820-3831

    • DOI

      10.1093/molbev/msab159

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Origin of symbiotic gut spirochetes as key players in the nutrition of termites2021

    • Author(s)
      Tokuda Gaku
    • Journal Title

      Environmental Microbiology

      Volume: 23 Issue: 8 Pages: 4092-4097

    • DOI

      10.1111/1462-2920.15625

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Increased Mutation Rate Is Linked to Genome Reduction in Prokaryotes2020

    • Author(s)
      Bourguignon Thomas、Kinjo Yukihiro、Villa-Martin Paula、Coleman Nicholas V.、Tang Qian、Arab Daej A.、Wang Zongqing、Tokuda Gaku、Hongoh Yuichi、Ohkuma Moriya、Ho Simon Y.W.、Pigolotti Simone、Lo Nathan
    • Journal Title

      Current Biology

      Volume: 30 Issue: 19 Pages: 3848-3855.e4

    • DOI

      10.1016/j.cub.2020.07.034

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Parallel reductive genome evolution in Desulfovibrio ectosymbionts independently acquired by Trichonympha protists in the termite gut2020

    • Author(s)
      Takeuchi Mariko、Kuwahara Hirokazu、Murakami Takumi、Takahashi Kazuki、Kajitani Rei、Toyoda Atsushi、Itoh Takehiko、Ohkuma Moriya、Hongoh Yuichi
    • Journal Title

      The ISME Journal

      Volume: 14 Issue: 9 Pages: 2288-2301

    • DOI

      10.1038/s41396-020-0688-1

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Characterization of CBM36-containing GH11 endoxylanase NtSymX11 from the hindgut metagenome of higher termite Nasutitermes takasagoensis displaying prominent catalytic activity.2019

    • Author(s)
      Kitamoto, M., Tokuda, G., Watanabe, H., and Arioka, M.
    • Journal Title

      Carbohydr. Res.

      Volume: 474 Pages: 1-7

    • DOI

      10.1016/j.carres.2019.01.003

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Duplication and soldier-specific expression of geranylgeranyl diphosphate synthase genes in a nasute termite Nasutitermes takasagoensis2019

    • Author(s)
      Hojo Masaru、Shigenobu Shuji、Maekawa Kiyoto、Miura Toru、Tokuda Gaku
    • Journal Title

      Insect Biochemistry and Molecular Biology

      Volume: 111 Pages: 103177-103177

    • DOI

      10.1016/j.ibmb.2019.103177

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Plant cell wall degradation in insects: Recent progress on endogenous enzymes revealed by multi-omics technologies2019

    • Author(s)
      Tokuda Gaku
    • Journal Title

      Advances in Insect Physiology

      Volume: 57 Pages: 97-136

    • DOI

      10.1016/bs.aiip.2019.08.001

    • ISBN
      9780128186602
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Parallel and Gradual Genome Erosion in the Blattabacterium Endosymbionts of Mastotermes darwiniensis and Cryptocercus Wood Roaches2018

    • Author(s)
      Kinjo Yukihiro、Bourguignon Thomas、Tong Kwei Jun、Kuwahara Hirokazu、Lim Sang Jin、Yoon Kwang Bae、Shigenobu Shuji、Park Yung Chul、Nalepa Christine A、Hongoh Yuichi、Ohkuma Moriya、Lo Nathan、Tokuda Gaku
    • Journal Title

      Genome Biology and Evolution

      Volume: 10 Issue: 6 Pages: 1622-1630

    • DOI

      10.1093/gbe/evy110

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fiber-associated spirochetes are major agents of hemicellulose degradation in the hindgut of wood-feeding higher termites2018

    • Author(s)
      Tokuda Gaku、Mikaelyan Aram、Fukui Chiho、Matsuura Yu、Watanabe Hirofumi、Fujishima Masahiro、Brune Andreas
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 115 Issue: 51

    • DOI

      10.1073/pnas.1810550115

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] シロアリ消化システムの変遷2017

    • Author(s)
      徳田 岳
    • Journal Title

      生物科学

      Volume: 68(3) Pages: 142-153

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] シロアリ社会性の発達と木質消化2017

    • Author(s)
      徳田 岳
    • Journal Title

      昆虫と自然

      Volume: 52(7) Pages: 38-40

    • Related Report
      2017 Annual Research Report
  • [Presentation] スギオシロアリにおける後腸内微生物群集の実験的改変と腸内代謝物の変化2022

    • Author(s)
      徳田 岳・関根麗子・福田真嗣
    • Organizer
      日本微生物生態学会第35回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Relationship between microbial structure and metabolic activities in the termite, Neotermes sugioi2022

    • Author(s)
      Gaku Tokuda
    • Organizer
      19th Congress of the International Union for the Study of Social Insects
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Metabolomic profiles in the hindguts of the wood-feeding cockroaches collected from East Asia and North America2022

    • Author(s)
      Tokuda, G., Kihara, K., Kitade, O., Fukuda, S.
    • Organizer
      XXVI International Congress of Entomology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] キゴキブリ腸内原生生物に共生する細菌の群集構造及び局在の解析2022

    • Author(s)
      丸岡 直弥, 猪飼桂, 工藤 凜平, 小野内 思有, 桑原 宏和, 大熊 盛也, 本郷 裕一
    • Organizer
      日本微生物生態学会第35回大会
    • Related Report
      2021 Annual Research Report
  • [Presentation] シロアリ後腸内微生物叢の実験的改変と後腸内生理活性の変化2022

    • Author(s)
      徳田 岳・関根麗子
    • Organizer
      第66回日本応用動物昆虫学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] The functional evolution of termite gut microbiota2021

    • Author(s)
      Bourguignon, T., Arora, J., Kinjo, Y., Sobotonik, J., Bucek, A., Stiblik, P., Roisin, Y., Zifcakova, L., Park, Y. C., Kim, K. Y., Sillam-Dusses, D., Herve, V., Lo, N., Tokuda, G., Brune, A.
    • Organizer
      日本微生物生態学会第34回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 下等シロアリの腸内微生物改変の試み2021

    • Author(s)
      徳田 岳・関根麗子
    • Organizer
      第65回日本応用動物昆虫学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] シロアリの木材消化における宿主と腸内微生物の役割分担2021

    • Author(s)
      徳田 岳
    • Organizer
      第2回オンライン基礎昆虫学会議
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] キゴキブリ共生原生生物の機能理解に向けたメタボローム解析の試み2020

    • Author(s)
      徳田 岳・木原久美子・北出 理・福田真嗣
    • Organizer
      令和2年度蚕糸・昆虫機能利用学術講演会
    • Related Report
      2019 Annual Research Report
  • [Presentation] タカサゴシロアリ後腸内の木片に付着したバクテリア群集間における木質分解酵素遺伝子の差異2020

    • Author(s)
      徳田 岳
    • Organizer
      第64回日本応用動物昆虫学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] シロアリと腸内微生物による木材消化の仕組み2019

    • Author(s)
      徳田 岳
    • Organizer
      日本土壌動物学会第42回大会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] A division of labor between termites and the gut symbionts for lignocellulose degradation2019

    • Author(s)
      Gaku Tokuda
    • Organizer
      OIST Mini Symposium “Ecology and Evolution of Termite Gut Microbes”
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 地域集団間におけるキゴキブリ腸内代謝物量の予備的検討2019

    • Author(s)
      徳田 岳・木原久美子・福田真嗣
    • Organizer
      第63回日本応用動物昆虫学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Sprirochaetes contribute to xylan degradation in the hindgut of wood-feeding higher termites2018

    • Author(s)
      Tokuda, G., Mikaelyan, A., Fukui, C., Matsuura, Y., Watanabe, H., Fujishima, M., Brune, A.
    • Organizer
      The 17th International Symposium on Microbial Ecology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Transition of symbiotic lifestyles by gene loss or gain: Blattabacterium in cockroaches-lower termites and spirochetes in higher termites2018

    • Author(s)
      Tokuda, G.
    • Organizer
      Max Planck Institute Seminar Series in Molecular, Cellular, and Environmental Microbiology
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] シロアリと腸内微生物による木材の消化と共生2018

    • Author(s)
      徳田 岳
    • Organizer
      日本微生物生態学会第32回大会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 韓国産キゴキブリCryptocercus kyebangensisの共生原生生物組成2018

    • Author(s)
      北出 理・Yung Chul Park・徳田 岳
    • Organizer
      日本生態学会第65回大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ムカシシロアリおよびキゴキブリの細胞内共生細菌ゲノムの平行退縮進化2018

    • Author(s)
      金城幸宏・Thomas Bourguignon・本郷裕一・Nathan Lo・徳田 岳
    • Organizer
      平成30年度 蚕糸・昆虫機能利用学術講演会
    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2024-01-30  

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