| Budget Amount *help |
¥42,380,000 (Direct Cost: ¥32,600,000、Indirect Cost: ¥9,780,000)
Fiscal Year 2020: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2019: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
Fiscal Year 2018: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Fiscal Year 2017: ¥11,310,000 (Direct Cost: ¥8,700,000、Indirect Cost: ¥2,610,000)
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| Outline of Final Research Achievements |
This study focused on the methyl donor S-adenosylmethionine that is produced through the methionine cycle and aimed to elucidate the molecular mechanism underlying the relationship between nutrient condition and organism lifespan from the viewpoint of RNA methylation. By using Caenorhabditis elegans as an aging model, we established a comprehensive analysis of endogenous RNA methylation. Briefly, total RNA of C. elegans was separated into low molecular weight RNA fraction, 26S and 18S rRNAs, then these were digested by nuclease/phosphatase, and the yielded methylnucleosides were quantitatively analyzed by using LC-MS/MS. We also identified a novel RNA methyltransferase and revealed that it is responsible for methylation at N1 of tRNA adenosine 58. Furthermore, we demonstrated that knockdown of this enzyme results in an increased lifespan compared with wild-type. Collectively, these findings suggest a possible involvement of tRNA methylation in the regulation of organism lifespan.
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