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Identification and characterization of a potential drug target regulating the pace of the central circadian clock in the brain through orphan G protein-coupled receptors

Research Project

Project/Area Number 17H01524
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field Biological pharmacy
Research InstitutionKyoto University

Principal Investigator

Doi Masao  京都大学, 薬学研究科, 教授 (20432578)

Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥41,080,000 (Direct Cost: ¥31,600,000、Indirect Cost: ¥9,480,000)
Fiscal Year 2021: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2020: ¥8,320,000 (Direct Cost: ¥6,400,000、Indirect Cost: ¥1,920,000)
Fiscal Year 2019: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Fiscal Year 2018: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
Fiscal Year 2017: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
Keywords体内時計 / G蛋白質共役受容体 / 生体時計 / 時計遺伝子 / N型糖鎖修飾
Outline of Final Research Achievements

Toward the development of biological rhythm regulators aimed at correcting lifestyle-related diseases, we found that a specific genome DNA sequence located in the non-coding region, which controls the expression of core clock gene, is essential for the maintenance of body's 24-h activity rhythm (Doi et al., Nat Commun 2019); showed that N-linked glycosylation occurs on the orphan G-protein-coupled receptor Gpr176, which is a period regulator of the master clock in the brain (Wang et al., Sci Rep 2020); revealed Gpr19 as an orphan receptor required for proper phase-entrainment of the clock by light (Yamaguchi et al., Sci Rep 2021); identified the G-protein-coupled receptor Calcr as a component required for keeping proper circadian rhythm in body temperature (Goda & Doi et al., Genes Dev 2018); and discovered an approach that restores local circadian enzymatic activity rhythm in the eyes to alleviate age-associated evaporative dry eye disease (Sasaki et al., Nat Aging 2022).

Academic Significance and Societal Importance of the Research Achievements

体内時計の異常は、現代社会に見られる多くの生活習慣病の根底に潜む共通の病因となることがこれまで示されてきたが、体内時計を是正すると病気が治せるのかという逆向きの問が重要な医療課題として残されている。このような中、私共は、本研究の結果、眼局所での酵素活性リズムすなわち局所生体時計を正常化する方法により、これまで有効な治療法のなかった加齢性ドライアイを治療できること見出したほか、体内時計の中枢を狙った中枢リズム調整薬の標的候補として創薬可能性の高いG蛋白質共役受容体Gpr176, Gpr19, Calcrを見出した。体内時計を活用した病気の治療・臨床応用に向け重要な基礎を構築できたといえる。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (97 results)

All 2022 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (4 results) Journal Article (31 results) (of which Int'l Joint Research: 13 results,  Peer Reviewed: 20 results,  Open Access: 15 results) Presentation (59 results) (of which Int'l Joint Research: 18 results,  Invited: 22 results) Remarks (3 results)

  • [Int'l Joint Research] Taipei Medical University(その他の国・地域)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Florida State University(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] シンシナティ病院/カリフォルニア大学(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] シンシナティ病院(米国)

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 特集 温度を感じる脳と身体の科学 体温の日内リズム制御における概日時計機構の役割2022

    • Author(s)
      Takahito Miyake, Masao Doi
    • Journal Title

      BRAIN and NERVE

      Volume: 74 Issue: 2 Pages: 159-166

    • DOI

      10.11477/mf.1416202001

    • ISSN
      1344-8129, 1881-6096
    • Year and Date
      2022-02-01
    • Related Report
      2021 Annual Research Report
  • [Journal Article] Intracrine activity involving NAD-dependent circadian steroidogenic activity governs age-associated meibomian gland dysfunction2022

    • Author(s)
      Sasaki Lena、Hamada Yuki、Yarimizu Daisuke、Morinaga Hironobu、Nishimura Emi K.、Kudo Fujimi、Manabe Ichiro、Haraguchi Shogo、Sugiura Yuki、Suematsu Makoto、Kinoshita Shigeru、Machida Mamiko、Nakajima Takeshi、Kiyonari Hiroshi、Okamura Hitoshi、Yamaguchi Yoshiaki、Miyake Takahito、Doi Masao
    • Journal Title

      Nature Aging

      Volume: 2 Issue: 2 Pages: 105-114

    • DOI

      10.1038/s43587-021-00167-8

    • NAID

      120007191374

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Gpr19 is a circadian clock-controlled orphan GPCR with a role in modulating free-running period and light resetting capacity of the circadian clock2021

    • Author(s)
      Yoshiaki Yamaguchi, Iori Murai, Kaoru Goto, Shotaro Doi, Huihua Zhou, Genzui Setsu, Hiroyuki Shimatani, Hitoshi Okamura, Takahito Miyake, Masao Doi
    • Journal Title

      Scientific Reports

      Volume: 11 Issue: 1 Pages: 22406-22406

    • DOI

      10.1038/s41598-021-01764-8

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thermographic imaging of mouse across circadian time reveals body surface temperature elevation associated with non-locomotor body movements2021

    • Author(s)
      Hiroyuki Shimatani, Yuichi Inoue, Yota Maekawa, Takahito Miyake, Yoshiaki Yamaguchi, Masao Doi
    • Journal Title

      PLoS One

      Volume: 16 Issue: 5 Pages: e0252447-e0252447

    • DOI

      10.1371/journal.pone.0252447

    • Related Report
      2021 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 老化と体内時計:加齢による脳内中枢時計の機能低下を中心に2021

    • Author(s)
      濵田悠貴、山口賀章、土居雅夫
    • Journal Title

      Geriatric Medicine(老年医学)

      Volume: 59 Pages: 683-687

    • Related Report
      2021 Annual Research Report
  • [Journal Article] 時間生物学と医療の融合-現状と将来-2021

    • Author(s)
      土居雅夫
    • Journal Title

      Medical Science Digest

      Volume: 47 Pages: 6-8

    • Related Report
      2021 Annual Research Report
  • [Journal Article] Role of α2δ3 in Cellular Synchronization of the Suprachiasmatic Nucleus Under Constant Light Conditions2021

    • Author(s)
      Matsuo Masahiro、Seo Kazuyuki、Mizuguchi Naoki、Yamazaki Fumiyoshi、Urabe Shoichi、Yamada Naoto、Doi Masao、Tominaga Keiko、Okamura Hitoshi
    • Journal Title

      Neuroscience

      Volume: 461 Pages: 1-10

    • DOI

      10.1016/j.neuroscience.2021.02.016

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hyperpolyploidization of hepatocyte initiates preneoplastic lesion formation in the liver2021

    • Author(s)
      Lin Heng、Huang Yen-Sung、Fustin Jean-Michel、Doi Masao、Chen Huatao、Lai Hui-Huang、Lin Shu-Hui、Lee Yen-Lurk、King Pei-Chih、Hou Hsien-San、Chen Hao-Wen、Young Pei-Yun、Chao Hsu-Wen
    • Journal Title

      Nature Communications

      Volume: 12 Issue: 1 Pages: 645-645

    • DOI

      10.1038/s41467-020-20572-8

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Time-Restricted G-Protein Signaling Pathways via GPR176, Gz, and RGS16 Set the Pace of the Master Circadian Clock in the Suprachiasmatic Nucleus2020

    • Author(s)
      Nakagawa Shumpei、Nguyen Pham Khanh Tien、Shao Xinyan、Doi Masao
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 21 Issue: 14 Pages: 5055-5055

    • DOI

      10.3390/ijms21145055

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 時計遺伝子の転写のシス制御エレメントに点変異を入れると体内時計はどうなるのか2020

    • Author(s)
      Nguyen Pham Khanh Tien、土居雅夫
    • Journal Title

      生化学

      Volume: 92 Pages: 735-739

    • NAID

      40022394518

    • Related Report
      2020 Annual Research Report
  • [Journal Article] 変温動物の日内体温制御2020

    • Author(s)
      三宅 崇仁、土居 雅夫
    • Journal Title

      新学術領域研究 温度生物学ハンドブック

      Volume: NA

    • Related Report
      2020 Annual Research Report
  • [Journal Article] 体内時計の中枢を調節するG蛋白質共役型受容体2020

    • Author(s)
      嶋谷 寛之、土居 雅夫
    • Journal Title

      膜タンパク質工学ハンドブック エヌ・ティー・エス出版

      Volume: NA Pages: 344-348

    • Related Report
      2020 Annual Research Report
  • [Journal Article] Identification and functional characterisation of N-linked glycosylation of the orphan G protein-coupled receptor Gpr1762020

    • Author(s)
      Tianyu Wang, Shumpei Nakagawa, Takahito Miyake, Genzui Setsu, Sumihiro Kunisue, Kaoru Goto, Akira Hirasawa, Hitoshi Okamura, Yoshiaki Yamaguchi, Masao Doi
    • Journal Title

      Scientific Reports

      Volume: 10 Issue: 1 Pages: 4429-4429

    • DOI

      10.1038/s41598-020-61370-y

    • NAID

      120006822520

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Reconstitution of Organismal Liver Clock Function Requires Light.2019

    • Author(s)
      Takahito Miyake, Masao Doi
    • Journal Title

      Trends in endocrinology and metabolism

      Volume: 30 Issue: 9 Pages: 569-571

    • DOI

      10.1016/j.tem.2019.07.017

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Non-coding cis-element of Period2 is essential for maintaining organismal circadian behaviour and body temperature rhythmicity2019

    • Author(s)
      Doi Masao、Shimatani Hiroyuki、Atobe Yuta、Murai Iori、Hayashi Hida、Takahashi Yukari、Fustin Jean-Michel、Yamaguchi Yoshiaki、Kiyonari Hiroshi、Koike Nobuya、Yagita Kazuhiro、Lee Choogon、Abe Manabu、Sakimura Kenji、Okamura Hitoshi
    • Journal Title

      Nature Communications

      Volume: 10 Issue: 1 Pages: 2563-2563

    • DOI

      10.1038/s41467-019-10532-2

    • NAID

      120006644061

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 昼寝の時刻の体温を制御する脳内中枢時計の分子機構2019

    • Author(s)
      前川 洋太、土居 雅夫
    • Journal Title

      内分泌・糖尿病・代謝内科

      Volume: 48 Pages: 415-419

    • Related Report
      2019 Annual Research Report
  • [Journal Article] Two Ck1δ transcripts regulated by m6A methylation code for two antagonistic kinases in the control of the circadian clock2018

    • Author(s)
      Fustin Jean-Michel、Kojima Rika、Itoh Kakeru、Chang Hsin-Yi、Ye Shiqi、Zhuang Bowen、Oji Asami、Gibo Shingo、Narasimamurthy Rajesh、Virshup David、Kurosawa Gen、Doi Masao、Manabe Ichiro、Ishihama Yasushi、Ikawa Masahito、Okamura Hitoshi
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 115 Issue: 23 Pages: 5980-5985

    • DOI

      10.1073/pnas.1721371115

    • NAID

      120006957427

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Fetal Leydig cells dedifferentiate and serve as adult Leydig stem cells2018

    • Author(s)
      Shima Yuichi、Miyabayashi Kanako、Sato Tetsuya、Suyama Mikita、Ohkawa Yasuyuki、Doi Masao、Okamura Hitoshi、Suzuki Kentaro
    • Journal Title

      Development

      Volume: 145 Issue: 23 Pages: 169136-169136

    • DOI

      10.1242/dev.169136

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 体内時計の中枢を調節するG蛋白質共役型受容体シグナル2018

    • Author(s)
      中川 峻平, 土居雅夫
    • Journal Title

      医学のあゆみ

      Volume: 267 Pages: 429-433

    • Related Report
      2018 Annual Research Report
  • [Journal Article] 特集企画 生体リズムを基盤とした時間医薬科学の展開2018

    • Author(s)
      土居雅夫
    • Journal Title

      医学のあゆみ

      Volume: 267 Pages: 427-427

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Calcitonin receptors are ancient modulators for rhythms of preferential temperature in insects and body temperature in mammals2018

    • Author(s)
      Goda Tadahiro、Doi Masao、Umezaki Yujiro、Murai Iori、Shimatani Hiroyuki、Chu Michelle L.、Nguyen Victoria H.、Okamura Hitoshi、Hamada Fumika N.
    • Journal Title

      Genes & Development

      Volume: 32 Issue: 2 Pages: 140-155

    • DOI

      10.1101/gad.307884.117

    • NAID

      120006401705

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Circadian coordination of ATP release in the urothelium via connexin43 hemichannels2018

    • Author(s)
      Sengiku A, Ueda M, Kono J, Sano T, Nishikawa N, Kunisue S, Tsujihana K, Liou L, Kanematsu A, Shimba S, Doi M, Okamura H, Ogawa O, and Negoro H
    • Journal Title

      Sci. Rep.

      Volume: 8 Issue: 1 Pages: 1996-1996

    • DOI

      10.1038/s41598-018-20379-0

    • NAID

      120006510288

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] <b>Circadian rhythms of micturition during jet </b><b>lag </b>2018

    • Author(s)
      Ito K, Yasuda M, Maeda Y, Fustin JM, Yamaguchi Y, Kono Y, Negoro H, Kanematsu A, Ogawa O, Doi M, and Okamura H
    • Journal Title

      Biomedical Research

      Volume: 39 Issue: 2 Pages: 57-63

    • DOI

      10.2220/biomedres.39.57

    • NAID

      130006708567

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of daily light on c-Fos expression in the suprachiasmatic nucleus under the jet lag condition.2018

    • Author(s)
      Chen Y, Yamaguchi Y, Suzuki T, Doi M, and Okamura H
    • Journal Title

      Acta Histochem. Cytochem.

      Volume: 印刷中

    • NAID

      130006727962

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Circadian PER2 protein oscillations do not persist in cycloheximide-treated mouse embryonic fibroblasts in culture2018

    • Author(s)
      Tainaka Motomi、Doi Masao、Inoue Yuichi、Murai Iori、Okamura Hitoshi
    • Journal Title

      Chronobiology International

      Volume: 35 Issue: 1 Pages: 132-136

    • DOI

      10.1080/07420528.2017.1316731

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 「昼寝」の体温リズムを調整する遺伝子を発見2018

    • Author(s)
      土居 雅夫
    • Journal Title

      化学

      Volume: 73 Pages: 73-73

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Gz-linked orphan GPCR regulating the center of the circadian clock2017

    • Author(s)
      國末 純宏, 土居 雅夫
    • Journal Title

      生化学

      Volume: 89 Issue: 5 Pages: 752-755

    • DOI

      10.14952/SEIKAGAKU.2017.890752

    • NAID

      40021377448

    • ISSN
      0037-1017
    • Year and Date
      2017-10-25
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Circadian clock regulates hepatic polyploidy by modulating Mkp1-Erk1/2 signaling pathway.2017

    • Author(s)
      Chao HW, Doi M, Fustin JM, Chen H, Murase K, Maeda Y, Hayashi H, Tanaka R, Sugawa M, Mizukuchi N, Yamaguchi Y, Yasunaga J, Matsuoka M, Sakai M, Matsumoto M, Hamada S, and Okamura H
    • Journal Title

      Nature Commun.

      Volume: 8 Issue: 1 Pages: 2238-2238

    • DOI

      10.1038/s41467-017-02207-7

    • NAID

      120006373904

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] G-protein-coupled receptor signaling through Gpr176, Gz, and RGS16 tunes time in the center of the circadian clock [Review]2017

    • Author(s)
      Goto Kaoru、Doi Masao、Wang Tianyu、Kunisue Sumihiro、Murai Iori、Okamura Hitoshi
    • Journal Title

      Endocrine Journal

      Volume: 64 Issue: 6 Pages: 571-579

    • DOI

      10.1507/endocrj.EJ17-0130

    • NAID

      130007307959

    • ISSN
      0918-8959, 1348-4540
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] <i><b>Gm7325</b></i><b> is MyoD-dependently expressed in activated muscle satellite </b><b>cells </b>2017

    • Author(s)
      Takei D, Nishi M, Fukada S, Doi M, Okamura H, Uezumi A, Zhang L, Yoshida M, Miyazato M, Ichimura A, Takeshima H.
    • Journal Title

      Biomedical Research

      Volume: 38 Issue: 3 Pages: 215-219

    • DOI

      10.2220/biomedres.38.215

    • NAID

      130006865614

    • ISSN
      0388-6107, 1880-313X
    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Carbachol induces phase-dependent phase shifts of Per1 transcription rhythms in cultured suprachiasmatic nucleus slices.2017

    • Author(s)
      Dojo K, Yamaguchi Y, Fustin JM, Doi M, Kobayashi M, and Okamura H
    • Journal Title

      J. Biol. Rhythms

      Volume: 32 Issue: 2 Pages: 101-108

    • DOI

      10.1177/0748730417691205

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 組織局所ステロイド合成による加齢性マイボーム腺機能障害の時間薬物治療法的アプローチ2022

    • Author(s)
      濵田悠貴、佐々木玲奈、鑓水大介、土居雅夫
    • Organizer
      第142回日本薬学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 新奇のイントラクライン機構を介した加齢性眼疾患・ドライアイ症治療法の開発―NAD+要求性ステロイド合成のサーカディアンリズムが鍵―2022

    • Author(s)
      土居雅夫
    • Organizer
      第10回 AAA(Academy of Aging and CArdiovascular-Diabetes Research)
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Circadian clock: disease etiology and drug target exploration2021

    • Author(s)
      Masao Doi
    • Organizer
      The 5th Asian Forum on Chronobiology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Necessity of de novo protein translation for continuous PER2 oscillation2021

    • Author(s)
      Xinyan Shao, Takahito Miyake, and Masao Doi
    • Organizer
      The 5th Asian Forum on Chronobiology
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Time as medicine and disease etiology2021

    • Author(s)
      Masao Doi
    • Organizer
      The CFBT Summer Showcase, Manchester, UK
    • Related Report
      2021 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 微小な温度変化がもたらすmRNA翻訳速度調節と概日時計制御2021

    • Author(s)
      三宅崇仁、井ノ上雄一、土居雅夫
    • Organizer
      Biothermology Workshop - 2021's Annual Workshop
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 脳内中枢時計の時刻調節に関与する新規オーファン受容体Gpr192021

    • Author(s)
      山口賀章、土居雅夫
    • Organizer
      生理学研究所研究会 運動器/代謝系連関による生体機能制御とその変容の仕組み
    • Related Report
      2021 Annual Research Report
  • [Presentation] 生体リズムを基盤とした時間医薬科学の展開2021

    • Author(s)
      土居雅夫
    • Organizer
      日本薬学会東海支部主催特別講演会
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] 翻訳速度制御を介した体内時計のパラメトリック制御2021

    • Author(s)
      三宅崇仁、井ノ上雄一、土居雅夫
    • Organizer
      第44回日本分子生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 生理的体温変化による体内時計のパラメトリック制御2021

    • Author(s)
      三宅崇仁、井ノ上雄一、土居雅夫
    • Organizer
      第59回日本生物物理学会
    • Related Report
      2021 Annual Research Report
  • [Presentation] Thermographic imaging of mouse across circadian time reveals body surface temperature elevation associated with non-locomotor body movements2021

    • Author(s)
      三宅崇仁、嶋谷寛之、土居雅夫
    • Organizer
      第28回日本時間生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] A robust and sustained circadian rhythm of Per2::Luc expression in the mouse meibomian gland acinar cells2021

    • Author(s)
      グエン ファム カン ティエン、三宅崇仁、土居雅夫
    • Organizer
      第28回日本時間生物学会年会
    • Related Report
      2021 Annual Research Report
  • [Presentation] 高血圧症の病理・薬理における時間生物学視点2021

    • Author(s)
      土居雅夫
    • Organizer
      Premium Hypertension Conference
    • Related Report
      2021 Annual Research Report
    • Invited
  • [Presentation] Research for drug discovery aimed at circadian rhythm regulation by time-restricted gating of G-protein signaling2021

    • Author(s)
      Masao Doi
    • Organizer
      第85回日本循環器学会学術集会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] The E’-box of Per2 is essential for daily maintenance of organismal behavior and physiology.2020

    • Author(s)
      Masao Doi
    • Organizer
      SRBR 2020, Society for research on biological rhythms
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 時計遺伝子のシス制御エレメントが個体の活動や生理リズムの維持に与える影響の範囲・限界2020

    • Author(s)
      土居雅夫
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Circadian PER2 protein oscillations do not persist when de novo translation is inhibited in cultured mouse embryonic fibroblasts2020

    • Author(s)
      Xinyan Shao, Takahito Miyake, and Masao Doi
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 体内時計の温度同調機構:生理的な範囲の温度変化が培養マウス繊維芽細胞の分子時計に与える影響について2020

    • Author(s)
      井ノ上 雄一、三宅 崇仁、土居 雅夫
    • Organizer
      第43回日本分子生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 加齢性睡眠障害/早朝覚醒の鍵を握る生体リズム機構2020

    • Author(s)
      土居雅夫
    • Organizer
      AMED老化メカニズムの解明・制御プロジェクト「老化研究産学連携シンポジウム」
    • Related Report
      2020 Annual Research Report
  • [Presentation] 体内時計の時差への適応・光同調を担う視交叉上核Gタンパク質共役型受容体2020

    • Author(s)
      山口賀章、岡村 均、土居雅夫
    • Organizer
      第27回日本時間生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Role of N-glycosylation of GPR176: A potential link between N-glycosylation and human chronotype2020

    • Author(s)
      中川峻平、土居雅夫
    • Organizer
      第27回日本時間生物学会年会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 体内時計の制御に関わるGz共役型オーファン受容体Gpr176のN型糖鎖修飾型糖鎖修飾の役割2020

    • Author(s)
      王 甜宇、土居雅夫
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Gz共役型オーファン受容体Gpr176のハイスループットリガンドスクリーニング法の確立2020

    • Author(s)
      中川峻平、土居雅夫
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 脳内サーカディアンリズム中枢を制御する時間選択的Gタンパク質シグナル伝達経路GPR176-Gz-RGS16の役割2020

    • Author(s)
      土居雅夫
    • Organizer
      第93回日本生化学会大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Real-time FRET imaging of circadian kinase activity in ex vivo cultured SCN slices2020

    • Author(s)
      Youichi Tanaka, Masao Doi
    • Organizer
      第140回日本薬学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 昼寝の体温・代謝リズムを制御する脳内サーカディアンリズム中枢の分子機構2020

    • Author(s)
      土居雅夫
    • Organizer
      第34回日本糖尿病・肥満動物学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Time-restricted G-protein signaling through Gpr176/Gz/Rgs16 sets the pace of the central clock2019

    • Author(s)
      Masao Doi
    • Organizer
      V World Congress for Chronobiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] The orphan G protein-coupled receptor Gpr176 is N-glycosylated in the suprachiasmatic nucleus2019

    • Author(s)
      Tianyu Wang, Genzui Setsu, Masao Doi
    • Organizer
      V World Congress for Chronobiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The suprachiasmatic nucleus-enriched orphan G protein-coupled receptor Gpr176 exerts its cAMP-repressing activity through Gz2019

    • Author(s)
      Shumpei Nakagawa, Sumihiro Kunisue, Masao Doi
    • Organizer
      V World Congress for Chronobiology
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Identification and functional characterization of N-lglycosylation of the orphan Gz-linked receptor Gpr1762019

    • Author(s)
      Shumpei Nakagawa, Tianyu Wang, Masao Doi
    • Organizer
      The 11th Seoul-Kyoto-Osaka Joint Symposium on Pharmaceutical Sciences for Young Scientists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Optogenetic Assessment of Interneuronal Connectivity between the Central Circadian Clock Neurons2019

    • Author(s)
      Daichi Moriyasu, Yoshiaki Yamaguchi, Masao Doi
    • Organizer
      The 11th Seoul-Kyoto-Osaka Joint Symposium on Pharmaceutical Sciences for Young Scientists
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Per2遺伝子プロモーターシス調節配列E’-boxを欠損したマウス個体の脆弱な概日振動2019

    • Author(s)
      土居雅夫
    • Organizer
      第26回日本時間生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Period2遺伝子シス調節エレメントE’-boxを欠損したマウス個体の脆弱な概日振動2019

    • Author(s)
      嶋谷寛之, 跡部祐太, 土居雅夫, 岡村均
    • Organizer
      第26回日本時間生物学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Non-coding cis-regulatory element E’-box of Period2 is essential for daily maintenance of organismal behavior and physiology2019

    • Author(s)
      Masao Doi
    • Organizer
      第3回日本循環器学会基礎研究フォーラム
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] The circadian clock regulator Gpr176 undergoes N-glycosylation and couples to Gz2019

    • Author(s)
      Masao Doi
    • Organizer
      第15回GPCR研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Circadian rhythms in nicotinamide adenine dinucleotide concentration in mouse liver2019

    • Author(s)
      Aya Shimada, Hiroki Nakamura, Daisuke Yarimizu, Masao Doi
    • Organizer
      The 9th Federation of the Asian and Oceanian Physiological Science Congress
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 時差環境下における視交叉上核の光応答性2019

    • Author(s)
      森安大地, 陳宇林, 山口賀章, 鈴木暢, 土居雅夫, 岡村均
    • Organizer
      日本薬学会第139年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] オーファンG蛋白質共役受容体シグナルの生化学を基軸とした生体リズム中枢調整薬の研究開発2019

    • Author(s)
      土居雅夫
    • Organizer
      生化学若い研究者の会 冬のセミナー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Calcitonin Receptor Modulates Body Temperature Rhythm in Mammals -Immunolocalization of the Calcitonin Receptor in Extra-SCN Regions-2018

    • Author(s)
      Yota Maekawa, Yoshiaki Yamaguchi, Iori Murai, Hiroyuki Shimatani, Hitoshi Okamura, Masao Doi
    • Organizer
      International Symposium on Biological Rhythms “20 Years since Discovery of mammalian Clock Genes”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Light Responsiveness in the Neurons of the Suprachiasmatic Nucleus under Jet Lag Condition2018

    • Author(s)
      Daichi Moriyasu, Yulin Chen, Yoshiaki Yamaguchi, Toru Suzuki, Masao Doi, Hitoshi Okamura
    • Organizer
      International Symposium on Biological Rhythms “20 Years since Discovery of mammalian Clock Genes”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Carbachol-induced phase-dependent phase-shift of core clock transcription rhythms in slice cultured suprachiasmatic nucleus2018

    • Author(s)
      Kumiko Dojo, Yoshiaki Yamaguchi, Jean-Michel Fustin, Masao Doi, Masaki Kobayashi, Hitoshi Okamura
    • Organizer
      The 9th Congress of Asian Sleep Research Society
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Calcitonin receptor modulates body temperature rhythm in mammals2018

    • Author(s)
      Hiroyuki Shimatani, Iori Murai, Masao Doi
    • Organizer
      International GPCR Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Development of high throughput screening assay method for Gz-linked orphan receptor Gpr1762018

    • Author(s)
      Tianyu Wang, Masao Doi
    • Organizer
      International GPCR Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Agonist-independent cAMP-repressing activity of the orphan receptor Gpr176 requires Gz2018

    • Author(s)
      Shumpei Nakagawa, Sumihiro Kunisue, Masao Doi
    • Organizer
      International GPCR Symposium
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 昼寝の体温を制御する脳内中枢時計の分子機構2018

    • Author(s)
      土居雅夫
    • Organizer
      第3回バイオサーモロジーワークショップ ー1℃の違いを知るー
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 昼寝の体温を制御するGPCR2018

    • Author(s)
      土居雅夫
    • Organizer
      生理学研究所研究会 運動器/代謝系連関による生体機能制御とその変容の仕組み
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 体内時計の中枢を制御するG蛋白質共役受容体シグナル2018

    • Author(s)
      土居雅夫
    • Organizer
      第22回睡眠科学研究講座
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] The detection of cAMP-repressing activity of an SCN-enriched orphan G-protein-coupled receptor Gpr1762018

    • Author(s)
      王甜宇, 土居雅夫
    • Organizer
      第41回日本神経科学大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Vipr2の視床における発現と網膜入力の影響2018

    • Author(s)
      村井伊織, 土居雅夫, 岡村均
    • Organizer
      第25回日本時間生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 時差環境下における日々の光刺激がSCNのc-Fos発現に及ぼす影響2018

    • Author(s)
      森安大地, 陳宇林, 山口賀章, 鈴木暢, 土居雅夫, 岡村均
    • Organizer
      第25回日本時間生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Immunohistological distribution of Calcitonin receptor in the brain:Where is a mediator of body temperature rhythm?2018

    • Author(s)
      Yota Maekawa1, Yoshiaki Yamaguchi1, Iori Murai1, Hitoshi Okamura1, Masao Doi
    • Organizer
      第25回日本時間生物学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 17.体温のサーカディアンリズムを制御する脳内中枢時計の分子機構2018

    • Author(s)
      土居雅夫
    • Organizer
      第65回日本実験動物学会総会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] Sleep Brain: Why do we need sleep?2017

    • Author(s)
      Masao Doi
    • Organizer
      The Japanese-American-German Frontiers of Science Symposium 2017,
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] G-protein coupled receptors in the SCN: Timed Gi/Gz signaling sets the pace of the central clock.2017

    • Author(s)
      Masao Doi
    • Organizer
      European Biological Rhythms Society Congress
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 脳内中枢時計によるG蛋白質共役受容体を介した体温の概日性制御機構2017

    • Author(s)
      土居雅夫
    • Organizer
      次世代脳プロジェクト冬のシンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] マウスの体温の日内変動パターンを規定するG蛋白質共役受容体の解析2017

    • Author(s)
      土居雅夫、村井伊織、嶋谷寛之、岡村 均
    • Organizer
      第24回日本時間生物学会年会、
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 生物時計を標的にした創薬戦略-生体リズムを調節するための鍵はどこにあるのか?-2017

    • Author(s)
      土居雅夫、岡村 均
    • Organizer
      日本睡眠学会第42回定期学術集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 生体リズム中枢における創薬標的分子探索2017

    • Author(s)
      土居雅夫、岡村 均
    • Organizer
      第39回日本生物学的精神医学会・第47回日本神経精神薬理学会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 体内時計の中枢を調節するG蛋白質共役型受容体シグナル機構2017

    • Author(s)
      土居雅夫、岡村 均
    • Organizer
      第90回日本内分泌学会学術総会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Remarks] 京都大学大学院薬学研究科医薬創成情報科学専攻システムバイオロジー分野

    • URL

      https://systems-biology.pharm.kyoto-u.ac.jp/

    • Related Report
      2021 Annual Research Report
  • [Remarks] 京都大学大学院薬学研究科医薬創成情報科学専攻システムバイオロジー分野

    • URL

      http://systems-biology.pharm.kyoto-u.ac.jp/

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report 2018 Annual Research Report
  • [Remarks] 京都大学大学院薬学研究科医薬創成情報科学専攻システムバイオロジー分野

    • URL

      http://www.pharm.kyoto-u.ac.jp/system-biology/doimasao/

    • Related Report
      2017 Annual Research Report

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Published: 2017-04-28   Modified: 2023-01-30  

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