• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Biological roles of novel proteolytic mechanism GOMED

Research Project

Project/Area Number 17H01533
Research Category

Grant-in-Aid for Scientific Research (A)

Allocation TypeSingle-year Grants
Section一般
Research Field General medical chemistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

shimizu shigeomi  東京医科歯科大学, 難治疾患研究所, 教授 (70271020)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥41,470,000 (Direct Cost: ¥31,900,000、Indirect Cost: ¥9,570,000)
Fiscal Year 2019: ¥14,430,000 (Direct Cost: ¥11,100,000、Indirect Cost: ¥3,330,000)
Fiscal Year 2018: ¥14,040,000 (Direct Cost: ¥10,800,000、Indirect Cost: ¥3,240,000)
Fiscal Year 2017: ¥13,000,000 (Direct Cost: ¥10,000,000、Indirect Cost: ¥3,000,000)
KeywordsGOMED / 新規オートファジー / ゴルジ体 / Atg5 / Ulk1 / オートファジー / RIPK3 / 生体分子 / シグナル伝達 / 細胞・組織
Outline of Final Research Achievements

The Golgi membrane-associated degradation pathway (GOMED) is a mechanism that degrades undelivered plasma membrane proteins and secretory proteins upon Golgi stress. GOMED is a unique and novel Golgi function involving dramatic morphological changes in the Golgi.
(1) We here identified five GOMED-associated molecules, and identified their signaling pathways. (2) We also clarified the role ofGOMED in neurons and pancreatic beta cells. (3) We developed the methods to detect GOMED using Ulk1 phosphorylation and small fluorescence compound. (4) We identified cytosolic accumulation of undelivered Golgi molecules, and which induces cell injury.

Academic Significance and Societal Importance of the Research Achievements

GOMED機能は、ホルモンやサイトカインなどの細胞外分泌や細胞膜レセプターの発現量を調節する機能として、我々が発見した新たな細胞機能です。本研究では、GOMED機構がどのようにして実行されるか、どの細胞で実行されるか、この機構が働かないと如何なる障害が起こるかを研究しました。その結果、神経細胞の維持やホルモン分泌制御に決定的な役割を果たしてることを発見しました。また、この機構の検出方法も明らかにし、今後病気への応用が期待されます。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • Research Products

    (28 results)

All 2020 2019 2018 2017 Other

All Int'l Joint Research (2 results) Journal Article (17 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 17 results,  Open Access: 11 results,  Acknowledgement Compliant: 1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 5 results) Remarks (1 results)

  • [Int'l Joint Research] Penn state University(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Yonsei University(韓国)

    • Related Report
      2019 Annual Research Report
  • [Journal Article] ER-resident sensor PERK is essential for mitochondrial thermogenesis in brown adipose tissue.2020

    • Author(s)
      Kato H, Okabe K, Miyake M, Hattori K, Fukaya T, Tanimoto K, Beini S, Mizuguchi M, Torii S, Arakawa S, Ono M, Saito Y, Sugiyama T, Funatsu T, Sato K, Shimizu S, Oyadomari S, Ichijo H, Kadowaki H, Nishitoh H.
    • Journal Title

      Life science alliance

      Volume: 3 Issue: 3 Pages: 201900576-201900576

    • DOI

      10.26508/lsa.201900576

    • NAID

      120006980545

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Identification of a phosphorylation site on Ulk1 required for genotoxic stress-induced alternative autophagy2020

    • Author(s)
      Torii Satoru、Yamaguchi Hirofumi、Nakanishi Akira、Arakawa Satoko、Honda Shinya、Moriwaki Kenta、Nakano Hiroyasu、Shimizu Shigeomi
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1 Pages: 1754-1754

    • DOI

      10.1038/s41467-020-15577-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Ceramide Analogue N-(1-hydroxy-3-morpholino-1-phenylpropan-2-yl)decanamide Induces Large Lipid Droplet Accumulation and Highlights the Effect of LAMP-2 Deficiency on Lipid Droplet Degradation2020

    • Author(s)
      Yuji Kato, Satoko Arakawa, Kazue Terasawa, Jin-Ichi Inokuchi, Takanori Iwata, Shigeomi Shimizu, Tetsuro Watabe, Miki Hara-Yokoyama
    • Journal Title

      Bioorganic & Medicinal Chemistry Letters

      Volume: 30 Issue: 3 Pages: 126891-126891

    • DOI

      10.1016/j.bmcl.2019.126891

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sanguisorba officinalis L. derived from herbal medicine prevents intestinal inflammation by inducing autophagy in macrophages.2020

    • Author(s)
      Yasueda A, Kayama H, Murohashi M, Nishimura J, Wakame K, Komatsu K, Ogino T, Miyoshi N, Takahashi H, Uemura M, Matsuda C, Kitagawa T, Takeda K, Ito T, Doki Y, Eguchi H, Shimizu S, Mizushima T
    • Journal Title

      Scientific reports

      Volume: press

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prediction of intracellular targets of a small compound by analyzing peptides presented on MHC class I2019

    • Author(s)
      Sugimoto Yuna、Murohashi Michiko、Arakawa Satoko、Honda Shinya、Shimizu Shigeomi
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 508 Issue: 2 Pages: 480-486

    • DOI

      10.1016/j.bbrc.2018.11.089

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Role of cyclooxygenase-2-mediated prostaglandin E2-prostaglandin E receptor 4 signaling in cardiac reprogramming2019

    • Author(s)
      Muraoka Naoto、Nara Kaori、Tamura Fumiya、Kojima Hidenori、Yamakawa Hiroyuki、Sadahiro Taketaro、Miyamoto Kazutaka、Isomi Mari、Haginiwa Sho、Tani Hidenori、Kurotsu Shota、Osakabe Rina、Torii Satoru、Shimizu Shigeomi、Okano Hideyuki、Sugimoto Yukihiko、Fukuda Keiichi、Ieda Masaki
    • Journal Title

      Nature Communications

      Volume: 10 Issue: 1 Pages: 674-674

    • DOI

      10.1038/s41467-019-08626-y

    • NAID

      120007128245

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Beclin 1 regulates recycling endosome and is required for skin development in mice2019

    • Author(s)
      Noguchi Saori、Honda Shinya、Saitoh Tatsuya、Matsumura Hiroyuki、Nishimura Emi、Akira Shizuo、Shimizu Shigeomi
    • Journal Title

      Communications Biology

      Volume: 2 Issue: 1 Pages: 37-37

    • DOI

      10.1038/s42003-018-0279-0

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Organelle zones in mitochondria2018

    • Author(s)
      Shimizu Shigeomi
    • Journal Title

      The Journal of Biochemistry

      Volume: 165 Issue: 2 Pages: 101-107

    • DOI

      10.1093/jb/mvy068

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Association Between Autophagy and Neurodegenerative Diseases.2018

    • Author(s)
      Nobuhiro Fujikake, Minkyoung Shin, Shigeomi Shimizu*
    • Journal Title

      Front. Neurosci.

      Volume: 12 Pages: 255-255

    • DOI

      10.3389/fnins.2018.00255

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The CCR4-NOT deadenylase complex controls Atg7-dependent cell death and heart function2018

    • Author(s)
      Yamaguchi Tomokazu et al
    • Journal Title

      Science Signaling

      Volume: 11 Issue: 516 Pages: 3638-3638

    • DOI

      10.1126/scisignal.aan3638

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Small fluorescent molecules for monitoring autophagic flux2018

    • Author(s)
      Iwashita Hidefumi、Sakurai Hajime Tajima、Nagahora Noriyoshi、Ishiyama Munetaka、Shioji Kosei、Sasamoto Kazumi、Okuma Kentaro、Shimizu Shigeomi、Ueno Yuichiro
    • Journal Title

      FEBS Letters

      Volume: 592 Issue: 4 Pages: 559-567

    • DOI

      10.1002/1873-3468.12979

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dram1 regulates DNA damage-induced alternative autophagy2018

    • Author(s)
      Nagata M, Arakawa S, Yamaguchi H, Torii S, Endo H, Tsujioka M, Honda S, Nishida Y, Konishi A, Shimizu S
    • Journal Title

      Cell Stress

      Volume: 2 Issue: 3 Pages: 55-65

    • DOI

      10.15698/cst2018.03.127

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Role of Atg5-dependent cell death in the embryonic development of Bax/Bak double-knockout mice2017

    • Author(s)
      Arakawa S, Tsujioka M, Yoshida T, Tajima-Sakurai H, Nishida Y, Matsuoka Y, Yoshino I, Tsujimoto Y, Shimizu S.
    • Journal Title

      Cell death and differ

      Volume: 9 Issue: 9 Pages: 1598-1608

    • DOI

      10.1038/cdd.2017.84

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Intrinsic autophagy is required for the maintenance of intestinal stem cells and for irradiation-induced intestinal regeneration.2017

    • Author(s)
      Asano J, Sato T, Ichinose S, Kajita M, Onai N, Shimizu S, Ohteki T.
    • Journal Title

      Cell reports

      Volume: 20 Issue: 5 Pages: 1050-1060

    • DOI

      10.1016/j.celrep.2017.07.019

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Live Cell Imaging of Mitochondrial Autophagy with a Novel Fluorescent Small Molecule2017

    • Author(s)
      Iwashita Hidefumi、Torii Satoru、Nagahora Noriyoshi、Ishiyama Munetaka、Shioji Kosei、Sasamoto Kazumi、Shimizu Shigeomi、Okuma Kentaro
    • Journal Title

      ACS Chemical Biology

      Volume: 12 Issue: 10 Pages: 2546-2551

    • DOI

      10.1021/acschembio.7b00647

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Rhodium-catalyzed odorless synthesis of diaryl sulfides from borylarenes and S-aryl thiosulfonates2017

    • Author(s)
      Kanemoto Kazuya、Sugimura Yasuyuki、Shimizu Shigeomi、Yoshida Suguru、Hosoya Takamitsu
    • Journal Title

      Chemical Communications

      Volume: 53 Issue: 77 Pages: 10640-10643

    • DOI

      10.1039/c7cc05868c

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hyperoxidation of ether-linked phospholipids accelerates neutrophil extracellular trap formation2017

    • Author(s)
      Yotsumoto Satoshi、Muroi Yuito、Chiba Tatsuya、Ohmura Rio、Yoneyama Maki、Magarisawa Megumi、Dodo Kosuke、Terayama Naoki、Sodeoka Mikiko、Aoyagi Ryohei、Arita Makoto、Arakawa Satoko、Shimizu Shigeomi、Tanaka Masato
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 16026-16026

    • DOI

      10.1038/s41598-017-15668-z

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 新規オートファジーによる インスリン分解機構2020

    • Author(s)
      清水重臣
    • Organizer
      7th Diabetes Research Meeting
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 2種類のオートファジーによる ミトコンドリアqualityの調節2020

    • Author(s)
      清水重臣
    • Organizer
      AMED村山班 TR会議
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 細胞死、ミトコンドリアと臓器不全2020

    • Author(s)
      清水重臣
    • Organizer
      第47回 日本集中治療医学会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 細胞死とオートファジーのクロストーク2019

    • Author(s)
      清水重臣
    • Organizer
      日本Cell Death学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Molecular mechanisms and physiological roles of Atg5-independent mitophagy2019

    • Author(s)
      Shigeomi Shimizu
    • Organizer
      ASMRM & J-mit
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] New insights into the mechanism of Golgi-mediated alternative autophagy using chemical biology approach2019

    • Author(s)
      Shigeomi Shimizu
    • Organizer
      International Symposium of Autophagy
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Atg5非依存的オートファジーの 分子機構と生理機2019

    • Author(s)
      清水重臣
    • Organizer
      第12回オートファジー研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Molecular mechanisms and physiological roles of alternative autophagy2019

    • Author(s)
      Shigeomi Shimizu
    • Organizer
      The 92nd Annual Meeting of the Japanese Biochemical Society
    • Related Report
      2019 Annual Research Report
  • [Remarks] 病態細胞生物ホームページ

    • URL

      http://www.tmd.ac.jp/mri/pcb/index.html

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi