Three types of motility mechanisms in pathogenic bacteria, class Mollicutes
| Project/Area Number |
17H01544
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| Research Category |
Grant-in-Aid for Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including mycology)
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| Research Institution | Osaka City University |
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Principal Investigator |
Miyata Makoto 大阪市立大学, 大学院理学研究科, 教授 (50209912)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000)
Fiscal Year 2019: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000)
Fiscal Year 2018: ¥13,780,000 (Direct Cost: ¥10,600,000、Indirect Cost: ¥3,180,000)
Fiscal Year 2017: ¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
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| Keywords | 細胞運動 / 光学顕微鏡 / クライオ電子顕微鏡 / 構造生物学 / 単粒子解析 / 合成細菌 / 電子線クライオトモグラフィー / 遺伝子操作 / 分子モーター / ナノバイオ / 電子顕微鏡 / 蛋白質 / 微生物 / 一分子計測 / 急速凍結レプリカ法 |
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| Outline of Final Research Achievements |
Class Mollicutes, pathogenic bacteria that have achieved their specific evolution through parasitism are featured by three unique motility mechanisms. In this study, we clarified the following facts. (i) Energy conversion in the motility mechanism. (ii) Structures of motors and legs forming the core parts of motility. (iii) Reproduction of spiroplasma swimming by transferring and expressing some genes in a synthetic bacterium created by Craig Venter Institute. These results have partly already been published as ten English articles. This study was developed into the JST CREST study led by Miyata, titled “Construction of a cell evolution model using the synthetic bacterium JCVI syn3.0B and genome manipulation”.
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| Academic Significance and Societal Importance of the Research Achievements |
地球上の細胞には全部で18種類の運動メカニズムが存在するが（Miyata M et al. Genes to Cells 2020 10.1111/gtc.12737），これまでにそのメカニズムが詳細に調べられたのは4つに過ぎなかった．本研究の進展によりさらに3つのメカニズムが加わることで，細胞運動能の法則や原理などが議論できるようになった．研究対象となっているモリクテス綱細菌はヒトや上海ガニなどの動物や，オレンジなどの植物に被害を与える病原菌で，運動能はそれらの感染性に必須である．本研究はこれらの被害を抑えるための基礎研究としても有用である．
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Report
(4 results)
Research Products
(179 results)
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[Journal Article] Tree of motility;A proposed history of motility systems in the tree of life2020
Author(s)
M Miyata, R C Robinson, T QP Uyeda, Y Fukumori, S Fukushima, S Haruta, M Homma, K Inaba, M Ito, C Kaito, K Kato, TKenri, Y Kinosita, S Kojima, T Minamino, H Mori, S Nakamura, D Nakane, K Nakayama, M Nishiyama, S Shibata, K Shimabukuro, M Tamakoshi, A Taoka, Y Tashiro, ITulum, HiWada, K Wakabayashi
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Journal Title
Genes to Cells
Volume: 25
Issue: 1
Pages: 6-21
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Defined chromosome structure in a genome-reduced Mycoplasma pneumoniae2017
Author(s)
Trussart M, Yus E, Martinez S, Bau D, Tahara YO, Pengo T, Widjaja M, Kretschmer S, Swoger J, Miyata M, Marti-Renom MA, Lluch-Senar M, Serrano L
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Journal Title
Nature Communications
Volume: 8
Issue: 1
Pages: 14665-14665
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Chemotaxis without conventional two-component system, based on cell polarity and aerobic conditions in helicity-switching swimming of Spiroplasma eriocheiris2017
Author(s)
Liu P, Zheng H, Meng Q, Terahara N, Gu W, Wang S, Zhao G, Nakane D, Wang W, Miyata M
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Journal Title
Frontiers in Microbiology
Volume: 8
Pages: 58-58
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] 生きものって何？2017
Author(s)
宮田 真人
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Journal Title
Seibutsu Butsuri
Volume: 57
Issue: 6
Pages: 283-283
DOI
NAID
ISSN
0582-4052, 1347-4219
Related Report
Open Access
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[Presentation] Bacterial actin homolog, MreBs and fibril are essential for the swimming motility of Spiroplasma eriocheiris2019
Author(s)
Ikuko Fujiwara, Daichi Takahashi, Yuya Sasajima, Hana Kiyama, Ayami Arakawa, K Kato, Tomoko Miyata, Shigeyuki Kakizawa, Keiichi Namba, Makoto Miyata
Organizer
11th Toyota Riken International Workshop on "Actin Filament: beyond the atomic resolution structures"
Related Report
Int'l Joint Research / Invited
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[Presentation] Structure of P1 adhesin, the leg for infection of Mycoplasma pneumoniae, as novel sialic acid receptor2018
Author(s)
U Matsumoto, Akihiro Kawamoto, Takayuki Kato, Miki Kinoshita, Yoshito Kawakita, Tsuyoshi Kenri, Shigetaro Mori, Keiichi Namba, Makoto Miyata
Organizer
9th OCARINA International Symposium
Related Report
Int'l Joint Research
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[Book] 動物学の百科事典2018
Author(s)
宮田 真人ほか
Total Pages
800
Publisher
丸善出版
ISBN
9784621303092
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