Epidemiologic research on the association between dietary habits, blood pressure, and metabolomics of blood and urine.

• Project/Area Number: 17H01553
• Research Category: Grant-in-Aid for Scientific Research (A)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Epidemiology and preventive medicine
• Research Institution: Shiga University of Medical Science
• Principal Investigator: 上島 弘嗣 滋賀医科大学, アジア疫学研究センター, 特任教授 (70144483)
• Co-Investigator(Kenkyū-buntansha): 三浦 克之 滋賀医科大学, 医学部, 教授 (90257452) ; 中川 秀昭 金沢医科大学, 総合医学研究所, 教授 (00097437) ; 坂田 清美 岩手医科大学, 医学部, 教授 (50225794) ; 斉藤 重幸 札幌医科大学, 保健医療学部, 教授 (60253994) ; 奥田 奈賀子 京都府立大学, 生命環境科学研究科, 教授 (80452233)
• Project Period (FY): 2017-04-01 – 2022-03-31
• Project Status: Granted (Fiscal Year 2021)
• Budget Amount: ¥41,600,000 (Direct Cost: ¥32,000,000、Indirect Cost: ¥9,600,000); Fiscal Year 2021: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2020: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2019: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000); Fiscal Year 2018: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2017: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
• Keywords: メタボローム解析 / 疫学 / 血圧 / 食習慣 / メタボローム / 公衆衛生 / 非感染性疾患 / 国際共同研究 / 公衆衛生学

Outline of Annual Research Achievements

2020年度は、INTERLIPID研究の1400血液検体のうち、残り230検体の核磁気共鳴分光法 (1HNMR) (MRC-NIHR National Phenome Centre、MetaBoss Technology Ltd) を用いたメタボローム測定を終えた。一方、英国Imperial College Londonにて引き続き実施される予定であった超高速液体クロマトグラフ質量分析計(UPLC-MS)を用いた質量分析は、2019年度末からのCOVID-19感染拡大の影響により作業が一時停止した。約1年遅れ、2020年12月にLC-MSデータが納品されたものの、代謝物同定作業が未実施の状態である。最終年度はこの同定作業、他データとの突合作業、クリーニング作業、解析作業を行う。その後、これらの研究成果としての学会発表や論文発表を行う。

Current Status of Research Progress

3: Progress in research has been slightly delayed.

Reason

2019年度末からのCOVID-19感染拡大およびそれに伴うロックダウンによる制限により、英国Imperial College Londonでのメタボローム測定作業が一時停止となった。LC-MSによるメタボローム測定に遅れが生じたこと、Imperial College Londonの共同研究者の来日や訪英が困難となったこと、国際学会が延期されたことなどから、全体的に遅れが生じている。

Strategy for Future Research Activity

LC-MS測定後の代謝物同定作業、他データとの突合作業、クリーニング作業、解析作業を行い、高血圧等の循環器疾患危険因子との関連を検討する。分析結果は、学会発表および論文発表を行う。

Research Products

• [Int'l Joint Research] Imperial College London(英国)
• [Int'l Joint Research] University of Hawaii/Northwestern University(米国)
• [Int'l Joint Research] Imperial College London(英国)
• [Int'l Joint Research] University of Hawaii(米国)
• [Int'l Joint Research] Imperial College London(英国)
• [Int'l Joint Research] University of Hawaii(米国)
• [Int'l Joint Research] Imperial College London(英国)
• [Int'l Joint Research] University of Hawaii(米国)
• [Journal Article] The association of fish consumption and its urinary metabolites with cardiovascular risk factors: the International Study of Macro-/Micronutrients and Blood Pressure (INTERMAP) (2020)
  • Author(s): Gibson Rachel、Lau Chung-Ho E、Loo Ruey Leng、Ebbels Timothy M D、Chekmeneva Elena、Dyer Alan R、Miura Katsuyuki、Ueshima Hirotsugu、Zhao Liancheng、Daviglus Martha L、Stamler Jeremiah、Van?Horn Linda、Elliott Paul、Holmes Elaine、Chan Queenie
  • Journal Title: The American Journal of Clinical Nutrition Volume: 111 Issue: 2 Pages: 280-290
  • DOI: 10.1093/ajcn/nqz293
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Association between plant-based diets and blood pressure in the INTERMAP study (2020)
  • Author(s): Aljuraiban Ghadeer、Chan Queenie、Gibson Rachel、Stamler Jeremiah、Daviglus Martha L、Dyer Alan R、Miura Katsuyuki、Wu Yangfeng、Ueshima Hirotsugu、Zhao Liancheng、Van Horn Linda、Elliott Paul、Oude Griep Linda M
  • Journal Title: BMJ Nutrition, Prevention & Health Volume: 3 Issue: 2 Pages: 133-142
  • DOI: 10.1136/bmjnph-2020-000077
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Food Sources of Dietary Potassium in the Adult Japanese Population: The International Study of Macro-/Micronutrients and Blood Pressure (INTERMAP) (2020)
  • Author(s): Okuda Nagako、Okayama Akira、Miura Katsuyuki、Yoshita Katsushi、Miyagawa Naoko、Saitoh Shigeyuki、Nakagawa Hideaki、Sakata Kiyomi、Chan Queenie、Elliott Paul、Ueshima Hirotsugu、Stamler Jeremiah
  • Journal Title: Nutrients Volume: 12 Issue: 3 Pages: 787-787
  • DOI: 10.3390/nu12030787
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Salt intake and prevalence of overweight/obesity in Japan, China, the United Kingdom, and the United States: the INTERMAP Study (2019)
  • Author(s): Zhou Long、Stamler Jeremiah、Chan Queenie、Van Horn Linda、Daviglus Martha L、Dyer Alan R、Miura Katsuyuki、Okuda Nagako、Wu Yangfeng、Ueshima Hirotsugu、Elliott Paul、Zhao Liancheng、INTERMAP Research Group
  • Journal Title: The American Journal of Clinical Nutrition Volume: 110 Issue: 1 Pages: 34-40
  • DOI: 10.1093/ajcn/nqz067
  • Peer Reviewed / Open Access
• [Journal Article] Agreement between 24-h dietary recalls and 24-h urine collections for estimating sodium intake in China, Japan, UK, USA (2019)
  • Author(s): Wen Xiaoxiao、Zhou Long、Stamler Jeremiah、Chan Queenie、Van Horn Linda、Daviglus Martha L.、Dyer Alan R.、Elliott Paul、Ueshima Hirotsugu、Miura Katsuyuki、Okuda Nagako、Wu Yangfeng、Zhao Liancheng
  • Journal Title: Journal of Hypertension Volume: 37 Issue: 4 Pages: 814-819
  • DOI: 10.1097/hjh.0000000000001941
  • Peer Reviewed / Open Access
• [Journal Article] Metabolic phenotyping for discovery of urinary biomarkers of diet, xenobiotics and blood pressure in the INTERMAP Study: an overview. (2017)
  • Author(s): Chan Q, Loo RL, Ebbels TM, Van Horn L, Daviglus ML, Stamler J, Nicholson JK, Holmes E, Elliott P
  • Journal Title: Hypertens Res. Volume: 40(4) Issue: 4 Pages: 336-345
  • DOI: 10.1038/hr.2016.164
  • Peer Reviewed / Int'l Joint Research