| Budget Amount *help |
¥40,430,000 (Direct Cost: ¥31,100,000、Indirect Cost: ¥9,330,000)
Fiscal Year 2021: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2020: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2019: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2018: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Fiscal Year 2017: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Outline of Final Research Achievements |
In bipolar patients and controls, paraventricular thalamic nucleus (PVT) were identified by calretinin staining. Double staining with anti-cytochrome c oxidase (COX) and anti-succinate dehydrogenase (SDH) antibodies was used to identify cells with mitochondrial dysfunction and anti-8-OHdG antibody immunostaining was used to evaluate DNA oxidation. Cells with mitochondrial dysfunction were identified in the PVT. We attempted to create a reporter mouse that expresses Cre specifically in the PVT by dividing Cre into two parts and expressing them as a fusion protein via a 2A sequence downstream of Gbx2 and Maob, and then aggregating them by drug administration. However, Cre activity was not obtained, and adeno-associated viruses were used in subsequent experiments.
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